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P14827

- MTEC_ENTCL

UniProt

P14827 - MTEC_ENTCL

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Protein
Modification methylase EcaI
Gene
ecaIM
Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3365. M.EcaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcaI (EC:2.1.1.72)
Short name:
M.EcaI
Alternative name(s):
Adenine-specific methyltransferase EcaI
Gene namesi
Name:ecaIM
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Modification methylase EcaI
PRO_0000087958Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP14827.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14827-1 [UniParc]FASTAAdd to Basket

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MAVGLNKKEI DGPASQRAVA CDLEPALPPI GARVTLNYPG KMDESIILQK    50
KDTKYLRVGS DFAKESSLIL PNSFIWSDNS LALNRLMVEG KKAKLIYLDP 100
PYATGMGFSS RSNEHAYDDC LTEAAYLEFM RRRLILMREI LDDDGTIYVH 150
IGHQMLGELK CLLDEIFGRE RFINLITRRK CSSKNSTKNN FANLNDYILC 200
YSKGKKYIWN RPLKKPDAEW LAKEYPKTDS KGQFKLVPIH APGVRHGATG 250
GEWKGMLPPP GKHWQYTPEK LDILDASGDI HWSKTGNPRR KVYLTDDKSI 300
GYTDYWEEFR DAHHQSILVT GYPTEKNFNM MKLIVGASSN PGDLVIDPFC 350
GSGSTLHAAS LLQRKWIGID ESLFAAKTVM KRFAIGRAPM GDYVNTSLNK 400
QTELPLSLNE TARHEYVSND FNIYVDELTA SVSKNELAEI QKAYRDLKAN 450
QQ 452
Length:452
Mass (Da):51,112
Last modified:April 1, 1990 - v1
Checksum:iC19D9582E4AE9535
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17111 Genomic DNA. Translation: CAA34968.1.
PIRiS07886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17111 Genomic DNA. Translation: CAA34968.1 .
PIRi S07886.

3D structure databases

ProteinModelPortali P14827.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3365. M.EcaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00506. D21N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and nucleotide sequence of the gene encoding the EcaI DNA methyltransferase."
    Brenner V., Venetianer P., Kiss A.
    Nucleic Acids Res. 18:355-359(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 30056.

Entry informationi

Entry nameiMTEC_ENTCL
AccessioniPrimary (citable) accession number: P14827
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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