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P14827 (MTEC_ENTCL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase EcaI

Short name=M.EcaI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase EcaI
Gene names
Name:ecaIM
OrganismEnterobacter cloacae
Taxonomic identifier550 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Protein attributes

Sequence length452 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 452452Modification methylase EcaI
PRO_0000087958

Sequences

Sequence LengthMass (Da)Tools
P14827 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: C19D9582E4AE9535

FASTA45251,112
        10         20         30         40         50         60 
MAVGLNKKEI DGPASQRAVA CDLEPALPPI GARVTLNYPG KMDESIILQK KDTKYLRVGS 

        70         80         90        100        110        120 
DFAKESSLIL PNSFIWSDNS LALNRLMVEG KKAKLIYLDP PYATGMGFSS RSNEHAYDDC 

       130        140        150        160        170        180 
LTEAAYLEFM RRRLILMREI LDDDGTIYVH IGHQMLGELK CLLDEIFGRE RFINLITRRK 

       190        200        210        220        230        240 
CSSKNSTKNN FANLNDYILC YSKGKKYIWN RPLKKPDAEW LAKEYPKTDS KGQFKLVPIH 

       250        260        270        280        290        300 
APGVRHGATG GEWKGMLPPP GKHWQYTPEK LDILDASGDI HWSKTGNPRR KVYLTDDKSI 

       310        320        330        340        350        360 
GYTDYWEEFR DAHHQSILVT GYPTEKNFNM MKLIVGASSN PGDLVIDPFC GSGSTLHAAS 

       370        380        390        400        410        420 
LLQRKWIGID ESLFAAKTVM KRFAIGRAPM GDYVNTSLNK QTELPLSLNE TARHEYVSND 

       430        440        450 
FNIYVDELTA SVSKNELAEI QKAYRDLKAN QQ 

« Hide

References

[1]"Cloning and nucleotide sequence of the gene encoding the EcaI DNA methyltransferase."
Brenner V., Venetianer P., Kiss A.
Nucleic Acids Res. 18:355-359(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 30056.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X17111 Genomic DNA. Translation: CAA34968.1.
PIRS07886.

3D structure databases

ProteinModelPortalP14827.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3365. M.EcaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 2 hits.
InterProIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00506. D21N6MTFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTEC_ENTCL
AccessionPrimary (citable) accession number: P14827
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries