Reviewed, UniProtKB/Swiss-Prot P14827 (MTEC_ENTCL)

Names and origin

Protein names

Recommended name:
    Modification methylase EcaI
      Short name=M.EcaI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase EcaI

Gene names

Name:

ecaIM

Organism

Enterobacter cloacae

Taxonomic identifier

550 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter › Enterobacter cloacae complex

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 452	452	Modification methylase EcaI		PRO_0000087958

Sequences

	Sequence		Length	Mass (Da)
	P14827-1 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: C19D9582E4AE9535	FASTA	452	51,112
	10 20 30 40 50 60 MAVGLNKKEI DGPASQRAVA CDLEPALPPI GARVTLNYPG KMDESIILQK KDTKYLRVGS 70 80 90 100 110 120 DFAKESSLIL PNSFIWSDNS LALNRLMVEG KKAKLIYLDP PYATGMGFSS RSNEHAYDDC 130 140 150 160 170 180 LTEAAYLEFM RRRLILMREI LDDDGTIYVH IGHQMLGELK CLLDEIFGRE RFINLITRRK 190 200 210 220 230 240 CSSKNSTKNN FANLNDYILC YSKGKKYIWN RPLKKPDAEW LAKEYPKTDS KGQFKLVPIH 250 260 270 280 290 300 APGVRHGATG GEWKGMLPPP GKHWQYTPEK LDILDASGDI HWSKTGNPRR KVYLTDDKSI 310 320 330 340 350 360 GYTDYWEEFR DAHHQSILVT GYPTEKNFNM MKLIVGASSN PGDLVIDPFC GSGSTLHAAS 370 380 390 400 410 420 LLQRKWIGID ESLFAAKTVM KRFAIGRAPM GDYVNTSLNK QTELPLSLNE TARHEYVSND 430 440 450 FNIYVDELTA SVSKNELAEI QKAYRDLKAN QQ

References

[1]	"Cloning and nucleotide sequence of the gene encoding the EcaI DNA methyltransferase." Brenner V., Venetianer P., Kiss A. Nucleic Acids Res. 18:355-359(1990) [PubMed: 2183182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 30056.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17111 Genomic DNA. Translation: CAA34968.1.
PIR	S07886.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3365. M.EcaI.
Enzyme and pathway databases
BRENDA	2.1.1.72. 1834.
Family and domain databases
InterPro	IPR002295. D21N6_MeTrfase. IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00506. D21N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTEC_ENTCL

Accession

Primary (citable) accession number: P14827

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	September 22, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)