Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P14827

- MTEC_ENTCL

UniProt

P14827 - MTEC_ENTCL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Modification methylase EcaI

Gene

ecaIM

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3365. M.EcaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcaI (EC:2.1.1.72)
Short name:
M.EcaI
Alternative name(s):
Adenine-specific methyltransferase EcaI
Gene namesi
Name:ecaIM
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 452452Modification methylase EcaIPRO_0000087958Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP14827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00506. D21N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14827-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVGLNKKEI DGPASQRAVA CDLEPALPPI GARVTLNYPG KMDESIILQK
60 70 80 90 100
KDTKYLRVGS DFAKESSLIL PNSFIWSDNS LALNRLMVEG KKAKLIYLDP
110 120 130 140 150
PYATGMGFSS RSNEHAYDDC LTEAAYLEFM RRRLILMREI LDDDGTIYVH
160 170 180 190 200
IGHQMLGELK CLLDEIFGRE RFINLITRRK CSSKNSTKNN FANLNDYILC
210 220 230 240 250
YSKGKKYIWN RPLKKPDAEW LAKEYPKTDS KGQFKLVPIH APGVRHGATG
260 270 280 290 300
GEWKGMLPPP GKHWQYTPEK LDILDASGDI HWSKTGNPRR KVYLTDDKSI
310 320 330 340 350
GYTDYWEEFR DAHHQSILVT GYPTEKNFNM MKLIVGASSN PGDLVIDPFC
360 370 380 390 400
GSGSTLHAAS LLQRKWIGID ESLFAAKTVM KRFAIGRAPM GDYVNTSLNK
410 420 430 440 450
QTELPLSLNE TARHEYVSND FNIYVDELTA SVSKNELAEI QKAYRDLKAN

QQ
Length:452
Mass (Da):51,112
Last modified:April 1, 1990 - v1
Checksum:iC19D9582E4AE9535
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17111 Genomic DNA. Translation: CAA34968.1.
PIRiS07886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17111 Genomic DNA. Translation: CAA34968.1 .
PIRi S07886.

3D structure databases

ProteinModelPortali P14827.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3365. M.EcaI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002295. D21N6_MeTrfase.
IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00506. D21N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and nucleotide sequence of the gene encoding the EcaI DNA methyltransferase."
    Brenner V., Venetianer P., Kiss A.
    Nucleic Acids Res. 18:355-359(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 30056.

Entry informationi

Entry nameiMTEC_ENTCL
AccessioniPrimary (citable) accession number: P14827
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 1, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3