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P14827

- MTEC_ENTCL

UniProt

P14827 - MTEC_ENTCL

Protein

Modification methylase EcaI

Gene

ecaIM

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3365. M.EcaI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase EcaI (EC:2.1.1.72)
    Short name:
    M.EcaI
    Alternative name(s):
    Adenine-specific methyltransferase EcaI
    Gene namesi
    Name:ecaIM
    OrganismiEnterobacter cloacae
    Taxonomic identifieri550 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 452452Modification methylase EcaIPRO_0000087958Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP14827.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR002295. D21N6_MeTrfase.
    IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00506. D21N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14827-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVGLNKKEI DGPASQRAVA CDLEPALPPI GARVTLNYPG KMDESIILQK    50
    KDTKYLRVGS DFAKESSLIL PNSFIWSDNS LALNRLMVEG KKAKLIYLDP 100
    PYATGMGFSS RSNEHAYDDC LTEAAYLEFM RRRLILMREI LDDDGTIYVH 150
    IGHQMLGELK CLLDEIFGRE RFINLITRRK CSSKNSTKNN FANLNDYILC 200
    YSKGKKYIWN RPLKKPDAEW LAKEYPKTDS KGQFKLVPIH APGVRHGATG 250
    GEWKGMLPPP GKHWQYTPEK LDILDASGDI HWSKTGNPRR KVYLTDDKSI 300
    GYTDYWEEFR DAHHQSILVT GYPTEKNFNM MKLIVGASSN PGDLVIDPFC 350
    GSGSTLHAAS LLQRKWIGID ESLFAAKTVM KRFAIGRAPM GDYVNTSLNK 400
    QTELPLSLNE TARHEYVSND FNIYVDELTA SVSKNELAEI QKAYRDLKAN 450
    QQ 452
    Length:452
    Mass (Da):51,112
    Last modified:April 1, 1990 - v1
    Checksum:iC19D9582E4AE9535
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17111 Genomic DNA. Translation: CAA34968.1.
    PIRiS07886.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X17111 Genomic DNA. Translation: CAA34968.1 .
    PIRi S07886.

    3D structure databases

    ProteinModelPortali P14827.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3365. M.EcaI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR002295. D21N6_MeTrfase.
    IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00506. D21N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and nucleotide sequence of the gene encoding the EcaI DNA methyltransferase."
      Brenner V., Venetianer P., Kiss A.
      Nucleic Acids Res. 18:355-359(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 30056.

    Entry informationi

    Entry nameiMTEC_ENTCL
    AccessioniPrimary (citable) accession number: P14827
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3