P14821 (GLB2A_ANAIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Globin-2 A chain Alternative name(s): Globin II A chain HBII-A
Organism	Anadara inaequivalvis (Inequivalve ark) (Scapharca inaequivalvis)
Taxonomic identifier	6561 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Bivalvia › Pteriomorphia › Arcoida › Arcoidea › Arcidae › Scapharca

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Heterotetramer of two alpha chains and two beta chains. Ref.5
Sequence similarities	Belongs to the globin family.

Ontologies

Keywords
Biological process	Oxygen transport Transport
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro oxygen binding Inferred from electronic annotation. Source: InterPro oxygen transporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.3

Chain

2 – 150

149

Globin-2 A chain

PRO_0000052488

Sites

Metal binding

102

1

Iron (heme proximal ligand)

Amino acid modifications

Modified residue

2

1

Blocked amino end (Val) Ref.3

Secondary structure

1

.

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.

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.

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.

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.

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150

Helix Strand Turn

Details...

Helix

5 – 10

6

Helix

13 – 26

14

Helix

27 – 29

3

Helix

30 – 44

15

Helix

46 – 55

10

Helix

58 – 63

6

Helix

65 – 83

19

Turn

84 – 86

3

Helix

88 – 104

17

Helix

109 – 112

4

Helix

113 – 115

3

Helix

116 – 127

12

Helix

128 – 130

3

Helix

133 – 149

17

Sequences

	Sequence		Length	Mass (Da)
	P14821 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 39B996380CBC0315	FASTA	150	16,238
	10 20 30 40 50 60 MVADAVAKVC GSEAIKANLR RSWGVLSADI EATGLMLMSN LFTLRPDTKT YFTRLGDVQK 70 80 90 100 110 120 GKANSKLRGH AITLTYALNN FVDSLDDPSR LKCVVEKFAV NHINRKISGD AFGAIVEPMK 130 140 150 ETLKARMGNY YSDDVAGAWA ALVGVVQAAL

References

[1]	"Cooperative homodimeric hemoglobin from Scapharca inaequivalvis. cDNA cloning and expression of the fully functional protein in E. coli." Gambacurta A., Piro M.C., Ascoli F. FEBS Lett. 330:90-94(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Scapharca inaequivalvis tetrameric hemoglobin A and B chains: cDNA sequencing and genomic organization." Piro M.C., Gambacurta A., Ascoli F. J. Mol. Evol. 43:594-601(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Scapharca hemoglobins, type cases of a novel mode of chain assembly and heme-heme communication. Amino acid sequence and subunit interactions of the tetrameric component." Petruzzelli R., Boffi A., Barra D., Bossa F., Ascoli F., Chiancone E. FEBS Lett. 259:133-136(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-150.
[4]	Gambacurta A. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 41-113.
[5]	"The 2.0 A crystal structure of Scapharca tetrameric hemoglobin: cooperative dimers within an allosteric tetramer." Royer W.E. Jr., Heard K.S., Harrington D.J., Chiancone E. J. Mol. Biol. 253:168-186(1995) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH HEME, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X71386 mRNA. Translation: CAA50509.1.
S83524 Genomic DNA. Translation: AAL96376.1.
X98566 Genomic DNA. Translation: CAA67176.1.

PIR

S39980.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HRR	X-ray	2.00	A/C/E/G	5-150	[»]
4HRT	X-ray	1.46	A/C/E/G	5-150	[»]

ProteinModelPortal

P14821.

SMR

P14821. Positions 2-150.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

1.10.490.10. 1 hit.

InterPro

IPR000971. Globin.
IPR009050. Globin-like.
IPR012292. Globin_dom.
[Graphical view]

Pfam

PF00042. Globin. 1 hit.
[Graphical view]

SUPFAM

SSF46458. Globin_like. 1 hit.

PROSITE

PS01033. GLOBIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14821.

Entry information

Entry name

GLB2A_ANAIN

Accession

Primary (citable) accession number: P14821

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)