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Protein

Glucoamylase

Gene

gla-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155SubstrateBy similarity1
Active sitei211Proton acceptorPROSITE-ProRule annotation1
Active sitei214Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:gla-1
ORF Names:B5O22.70, NCU01517
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 6, Linkage Group II

Organism-specific databases

EuPathDBiFungiDB:NCU01517.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Protein family/group databases

Allergomei10750. Neu cr Glucoamylase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000000146920 – 35Sequence analysisAdd BLAST16
ChainiPRO_000000147036 – 626GlucoamylaseAdd BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...)Sequence analysis1
Glycosylationi206N-linked (GlcNAc...)Sequence analysis1
Glycosylationi217N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Proteomic databases

PRIDEiP14804.

Structurei

3D structure databases

ProteinModelPortaliP14804.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini520 – 626CBM20PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated
Contains 1 CBM20 (carbohydrate binding type-20) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000182646.
InParanoidiP14804.
KOiK01178.
OrthoDBiEOG092C1YYH.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR011613. Glyco_hydro_15/PHK.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PIRSFiPIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLVSSLLVV GAAFQAVLGL PDPLHEKRHS DIIKRSVDSY IQTETPIAQK
60 70 80 90 100
NLLCNIGASG CRASGAASGV VVASPSKSSP DYWYTWTRDA ALVTKLIVDE
110 120 130 140 150
FTNDYNTTLQ NTIQAYAAAQ AKLQGVSNPS GSLSNGAGLG EPKFMVDLQQ
160 170 180 190 200
FTGAWGRPQR DGPPLRAIAL IGYGKWLVSN GYADTAKSII WPIVKNDLAY
210 220 230 240 250
TAQYWNNTGF DLWEEVNSSS FFTIAASHRA LVEGSAFAKS VGSSCSACDA
260 270 280 290 300
IAPQILCFQQ SFWSNSGYII SNFVNYRSGK DINSVLTSIH NFDPAAGCDV
310 320 330 340 350
NTFQPCSDRA LANHKVVVDS MRFWGVNSGR TAGKAAAVGR YAEDVYYNGN
360 370 380 390 400
PWYLATLAAA EQLYDAVYVW KKQGSITVTS TSLAFFKDLV PSVSTGTYSS
410 420 430 440 450
SSSTYTAIIN AVTTYADGFV DIVAQYTPSD GSLAEQFDKD SGAPLSATHL
460 470 480 490 500
TWSYASFLSA AARRAGIVPP SWGAASANSL PGSCSASTVA GSYATATATS
510 520 530 540 550
FPANLTPAST TVTPPTQTGC AADHEVLVTF NEKVTTSYGQ TVKVVGSIAA
560 570 580 590 600
LGNWAPASGV TLSAKQYSSS NPLWSTTIAL PQGTSFKYKY VVVNSDGSVK
610 620
WENDPDRSYA VGTDCASTAT LDDTWR
Length:626
Mass (Da):66,475
Last modified:April 30, 2003 - v3
Checksum:i54E5BDDBA7A3E349
GO

Sequence cautioni

The sequence EAA27730 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82Missing in CAA47707 (PubMed:8221928).Curated1
Sequence conflicti550A → R in CAA47707 (PubMed:8221928).Curated1
Sequence conflicti560V → L in CAA47707 (PubMed:8221928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67291 Genomic DNA. Translation: CAA47707.1.
AL355932 Genomic DNA. Translation: CAB91426.1.
CM002237 Genomic DNA. Translation: EAA27730.2. Different initiation.
PIRiS36364.
RefSeqiXP_956966.2. XM_951873.3.

Genome annotation databases

EnsemblFungiiEAA27730; EAA27730; NCU01517.
GeneIDi3873129.
KEGGincr:NCU01517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67291 Genomic DNA. Translation: CAA47707.1.
AL355932 Genomic DNA. Translation: CAB91426.1.
CM002237 Genomic DNA. Translation: EAA27730.2. Different initiation.
PIRiS36364.
RefSeqiXP_956966.2. XM_951873.3.

3D structure databases

ProteinModelPortaliP14804.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei10750. Neu cr Glucoamylase.
CAZyiCBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.

Proteomic databases

PRIDEiP14804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAA27730; EAA27730; NCU01517.
GeneIDi3873129.
KEGGincr:NCU01517.

Organism-specific databases

EuPathDBiFungiDB:NCU01517.

Phylogenomic databases

HOGENOMiHOG000182646.
InParanoidiP14804.
KOiK01178.
OrthoDBiEOG092C1YYH.

Family and domain databases

Gene3Di1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR011613. Glyco_hydro_15/PHK.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PIRSFiPIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSiPR00736. GLHYDRLASE15.
SMARTiSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMiSSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEiPS51166. CBM20. 1 hit.
PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMYG_NEUCR
AccessioniPrimary (citable) accession number: P14804
Secondary accession number(s): Q7RV62, Q9P5U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 30, 2003
Last modified: November 2, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.