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Protein

Glucoamylase

Gene

gla-1

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155SubstrateBy similarity1
Active sitei211Proton acceptorPROSITE-ProRule annotation1
Active sitei214Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiCBM20 Carbohydrate-Binding Module Family 20
GH15 Glycoside Hydrolase Family 15

Names & Taxonomyi

Protein namesi
Recommended name:
Glucoamylase (EC:3.2.1.3)
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene namesi
Name:gla-1
ORF Names:B5O22.70, NCU01517
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
Proteomesi
  • UP000001805 Componenti: Chromosome 6, Linkage Group II

Organism-specific databases

EuPathDBiFungiDB:NCU01517

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Protein family/group databases

Allergomei10750 Neu cr Glucoamylase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000000146920 – 35Sequence analysisAdd BLAST16
ChainiPRO_000000147036 – 626GlucoamylaseAdd BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi206N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi217N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Proteomic databases

PRIDEiP14804

Structurei

3D structure databases

ProteinModelPortaliP14804
SMRiP14804
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini520 – 626CBM20PROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the glycosyl hydrolase 15 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000182646
InParanoidiP14804
KOiK01178
OrthoDBiEOG092C1YYH

Family and domain databases

CDDicd05811 CBM20_glucoamylase, 1 hit
Gene3Di1.50.10.10, 1 hit
2.60.40.10, 1 hit
InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR012341 6hp_glycosidase-like_sf
IPR013784 Carb-bd-like_fold
IPR034836 CBM20_glucoamylase
IPR002044 CBM_fam20
IPR000165 Glucoamylase
IPR008291 Glucoamylase_SBD
IPR011613 Glyco_hydro_15/PHK
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00686 CBM_20, 1 hit
PF00723 Glyco_hydro_15, 1 hit
PIRSFiPIRSF001031 Glu-a-glcsd_SBD, 1 hit
PRINTSiPR00736 GLHYDRLASE15
SMARTiView protein in SMART
SM01065 CBM_2, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit
SSF49452 SSF49452, 1 hit
PROSITEiView protein in PROSITE
PS51166 CBM20, 1 hit
PS00820 GLUCOAMYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLVSSLLVV GAAFQAVLGL PDPLHEKRHS DIIKRSVDSY IQTETPIAQK
60 70 80 90 100
NLLCNIGASG CRASGAASGV VVASPSKSSP DYWYTWTRDA ALVTKLIVDE
110 120 130 140 150
FTNDYNTTLQ NTIQAYAAAQ AKLQGVSNPS GSLSNGAGLG EPKFMVDLQQ
160 170 180 190 200
FTGAWGRPQR DGPPLRAIAL IGYGKWLVSN GYADTAKSII WPIVKNDLAY
210 220 230 240 250
TAQYWNNTGF DLWEEVNSSS FFTIAASHRA LVEGSAFAKS VGSSCSACDA
260 270 280 290 300
IAPQILCFQQ SFWSNSGYII SNFVNYRSGK DINSVLTSIH NFDPAAGCDV
310 320 330 340 350
NTFQPCSDRA LANHKVVVDS MRFWGVNSGR TAGKAAAVGR YAEDVYYNGN
360 370 380 390 400
PWYLATLAAA EQLYDAVYVW KKQGSITVTS TSLAFFKDLV PSVSTGTYSS
410 420 430 440 450
SSSTYTAIIN AVTTYADGFV DIVAQYTPSD GSLAEQFDKD SGAPLSATHL
460 470 480 490 500
TWSYASFLSA AARRAGIVPP SWGAASANSL PGSCSASTVA GSYATATATS
510 520 530 540 550
FPANLTPAST TVTPPTQTGC AADHEVLVTF NEKVTTSYGQ TVKVVGSIAA
560 570 580 590 600
LGNWAPASGV TLSAKQYSSS NPLWSTTIAL PQGTSFKYKY VVVNSDGSVK
610 620
WENDPDRSYA VGTDCASTAT LDDTWR
Length:626
Mass (Da):66,475
Last modified:April 30, 2003 - v3
Checksum:i54E5BDDBA7A3E349
GO

Sequence cautioni

The sequence EAA27730 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82Missing in CAA47707 (PubMed:8221928).Curated1
Sequence conflicti550A → R in CAA47707 (PubMed:8221928).Curated1
Sequence conflicti560V → L in CAA47707 (PubMed:8221928).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67291 Genomic DNA Translation: CAA47707.1
AL355932 Genomic DNA Translation: CAB91426.1
CM002237 Genomic DNA Translation: EAA27730.2 Different initiation.
PIRiS36364
RefSeqiXP_956966.2, XM_951873.3

Genome annotation databases

EnsemblFungiiEAA27730; EAA27730; NCU01517
GeneIDi3873129
KEGGincr:NCU01517

Similar proteinsi

Entry informationi

Entry nameiAMYG_NEUCR
AccessioniPrimary (citable) accession number: P14804
Secondary accession number(s): Q7RV62, Q9P5U5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 30, 2003
Last modified: March 28, 2018
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health