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P14804 (AMYG_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucoamylase

EC=3.2.1.3
Alternative name(s):
1,4-alpha-D-glucan glucohydrolase
Glucan 1,4-alpha-glucosidase
Gene names
Name:gla-1
ORF Names:B5O22.70, NCU01517
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length626 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 15 family.

Contains 1 CBM20 (carbohydrate binding type-20) domain.

Sequence caution

The sequence EAA27730.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Propeptide20 – 3516 Potential
PRO_0000001469
Chain36 – 626591Glucoamylase
PRO_0000001470

Regions

Domain520 – 626107CBM20

Sites

Active site2111Proton acceptor By similarity
Active site2141Proton donor By similarity
Binding site1551Substrate By similarity

Amino acid modifications

Glycosylation1061N-linked (GlcNAc...) Potential
Glycosylation2061N-linked (GlcNAc...) Potential
Glycosylation2171N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict821Missing in CAA47707. Ref.1
Sequence conflict5501A → R in CAA47707. Ref.1
Sequence conflict5601V → L in CAA47707. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P14804 [UniParc].

Last modified April 30, 2003. Version 3.
Checksum: 54E5BDDBA7A3E349

FASTA62666,475
        10         20         30         40         50         60 
MHLVSSLLVV GAAFQAVLGL PDPLHEKRHS DIIKRSVDSY IQTETPIAQK NLLCNIGASG 

        70         80         90        100        110        120 
CRASGAASGV VVASPSKSSP DYWYTWTRDA ALVTKLIVDE FTNDYNTTLQ NTIQAYAAAQ 

       130        140        150        160        170        180 
AKLQGVSNPS GSLSNGAGLG EPKFMVDLQQ FTGAWGRPQR DGPPLRAIAL IGYGKWLVSN 

       190        200        210        220        230        240 
GYADTAKSII WPIVKNDLAY TAQYWNNTGF DLWEEVNSSS FFTIAASHRA LVEGSAFAKS 

       250        260        270        280        290        300 
VGSSCSACDA IAPQILCFQQ SFWSNSGYII SNFVNYRSGK DINSVLTSIH NFDPAAGCDV 

       310        320        330        340        350        360 
NTFQPCSDRA LANHKVVVDS MRFWGVNSGR TAGKAAAVGR YAEDVYYNGN PWYLATLAAA 

       370        380        390        400        410        420 
EQLYDAVYVW KKQGSITVTS TSLAFFKDLV PSVSTGTYSS SSSTYTAIIN AVTTYADGFV 

       430        440        450        460        470        480 
DIVAQYTPSD GSLAEQFDKD SGAPLSATHL TWSYASFLSA AARRAGIVPP SWGAASANSL 

       490        500        510        520        530        540 
PGSCSASTVA GSYATATATS FPANLTPAST TVTPPTQTGC AADHEVLVTF NEKVTTSYGQ 

       550        560        570        580        590        600 
TVKVVGSIAA LGNWAPASGV TLSAKQYSSS NPLWSTTIAL PQGTSFKYKY VVVNSDGSVK 

       610        620 
WENDPDRSYA VGTDCASTAT LDDTWR 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequence analysis of the glucoamylase gene of Neurospora crassa."
Stone P.J., Makoff A.J., Parish J.H., Radford A.
Curr. Genet. 24:205-211(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]"Exported proteins of Neurospora crassa: 1-glucoamylase."
Koh-Luar S.I., Parish J.H., Bleasby A.J., Pappin D.J.C., Ainley K., Johansen F.E., Radford A.
Enzyme Microb. Technol. 11:692-695(1989)
Cited for: PROTEIN SEQUENCE OF 36-65.
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X67291 Genomic DNA. Translation: CAA47707.1.
AL355932 Genomic DNA. Translation: CAB91426.1.
CM002237 Genomic DNA. Translation: EAA27730.2. Different initiation.
PIRS36364.
RefSeqXP_956966.1. XM_951873.2.
UniGeneNcr.420.

3D structure databases

ProteinModelPortalP14804.
SMRP14804. Positions 36-626.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5141.NCU01517.1.

Protein family/group databases

Allergome10750. Neu cr Glucoamylase.
CAZyCBM20. Carbohydrate-Binding Module Family 20.
GH15. Glycoside Hydrolase Family 15.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000001634; EFNCRP00000001634; EFNCRG00000001632.
GeneID3873129.
KEGGncr:NCU01517.

Phylogenomic databases

eggNOGCOG3387.
HOGENOMHOG000182646.
KOK01178.
OrthoDBEOG7NPG4B.

Family and domain databases

Gene3D1.50.10.10. 1 hit.
2.60.40.10. 1 hit.
InterProIPR008928. 6-hairpin_glycosidase-like.
IPR012341. 6hp_glycosidase.
IPR013784. Carb-bd-like_fold.
IPR002044. CBM_fam20.
IPR000165. Glucoamylase.
IPR008291. Glucoamylase_SBD.
IPR015902. Glyco_hydro_13.
IPR011613. Glyco_hydro_15.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00686. CBM_20. 1 hit.
PF00723. Glyco_hydro_15. 1 hit.
[Graphical view]
PIRSFPIRSF001031. Glu-a-glcsd_SBD. 1 hit.
PRINTSPR00736. GLHYDRLASE15.
SMARTSM01065. CBM_2. 1 hit.
[Graphical view]
SUPFAMSSF48208. SSF48208. 1 hit.
SSF49452. SSF49452. 1 hit.
PROSITEPS51166. CBM20. 1 hit.
PS00820. GLUCOAMYLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMYG_NEUCR
AccessionPrimary (citable) accession number: P14804
Secondary accession number(s): Q7RV62, Q9P5U5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 30, 2003
Last modified: March 19, 2014
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries