P14762 (CPXI_BACME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450(BM-1)
EC=1.14.14.-

Gene names

Name:

cyp106

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Cofactor	Heme group By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Cytochrome P450(BM-1)		PRO_0000052218
Sites
	Metal binding	356	1	Iron (heme axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	P14762 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: C9AE293E76745387	FASTA	410	47,461
	10 20 30 40 50 60 MNKEVIPVTE IPKFQSRAEE FFPIQWYKEM LNNSPVYFHE ETNTWNVFQY EHVKQVLSNY 70 80 90 100 110 120 DFFSSDGQRT TIFVGDNSKK KSTSPITNLT NLDPPDHRKA RSLLAAAFTP RSLKNWEPRI 130 140 150 160 170 180 KQIAADLVEA IQKNSTINIV DDLSSPFPSL VIADLFGVPV KDRYQFKKWV DILFQPYDQE 190 200 210 220 230 240 RLEEIEQEKQ RAGAEYFQYL YPIVIEKRSN LSDDIISDLI QAEVDGETFT DEEIVHATML 250 260 270 280 290 300 LLGAGVETTS HAIANMFYSF LYDDKSLYSE LRNNRELAPK AVEEMLRYRF HISRRDRTVK 310 320 330 340 350 360 QDNELLGVKL KKGDVVIAWM SACNMDETMF ENPFSVDIHR PTNKKHLTFG NGPHFCLGAP 370 380 390 400 410 LARLEMKIIL EAFLEAFSHI EPFEDFELEP HLTASATGQS LTYLPMTVYR

References

[1]

"Molecular cloning, coding nucleotides and the deduced amino acid sequence of P-450BM-1 from Bacillus megaterium." He J.S., Ruettinger R.T., Liu H.-M., Fulco A.J. Biochim. Biophys. Acta 1009:301-303(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-25. Strain: ATCC 14581 / DSM 32 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / VKM B-512.

[2]

"The molecular cloning and characterization of BM1P1 and BM1P2 proteins, putative positive transcription factors involved in barbiturate-mediated induction of the genes encoding cytochrome P450BM-1 of Bacillus megaterium." He J.S., Liang Q., Fulco A.J. J. Biol. Chem. 270:18615-18625(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16610 Genomic DNA. Translation: CAA34612.1. S79230 mRNA. Translation: AAC60495.1.
PIR	O4BS6M. S07764.
3D structure databases
ProteinModelPortal	P14762.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR002397. Cyt_P450_B. IPR017972. Cyt_P450_CS. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00359. BP450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CPXI_BACME

Accession

Primary (citable) accession number: P14762

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program