P14749 (AGAL_CYATE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-galactosidase EC=3.2.1.22 Alternative name(s): Alpha-D-galactoside galactohydrolase Melibiase
Organism	Cyamopsis tetragonoloba (Guar) (Cluster bean)
Taxonomic identifier	3832 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Indigofereae › Cyamopsis

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the hydrolysis of the galactomannan, it splits alpha-linked galactose moieties. It is particularly suitable for the hydrolysis of guar gum to a gum with improved gelling properties. Preferentially cleaves alpha-1,6 glycoside linkages.
Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Propeptide	25 – 47	23			PRO_0000001002
	Chain	48 – 411	364	Alpha-galactosidase		PRO_0000001003
Regions
	Region	210 – 214	5	Substrate binding By similarity
Sites
	Active site	177	1	Nucleophile By similarity
	Active site	232	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	32	1	N-linked (GlcNAc...) Potential
	Glycosylation	145	1	N-linked (GlcNAc...) Potential
	Glycosylation	352	1	N-linked (GlcNAc...) Potential
	Disulfide bond	68 ↔ 100		By similarity
	Disulfide bond	148 ↔ 179		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14749 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 5B1715858D1AB11E	FASTA	411	45,136
	10 20 30 40 50 60 MATHYSIIGG MIIVVLLMII GSEGGRLLEK KNRTSAEAEH YNVRRYLAEN GLGQTPPMGW 70 80 90 100 110 120 NSWNHFGCDI NENVVRETAD AMVSTGLAAL GYQYINLDDC WAELNRDSEG NMVPNAAAFP 130 140 150 160 170 180 SGIKALADYV HSKGLKLGVY SDAGNQTCSK RMPGSLGHEE QDAKTFASWG VDYLKYDNCE 190 200 210 220 230 240 NLGISVKERY PPMGKALLSS GRPIFFSMCE WGWEDPQIWA KSIGNSWRTT GDIEDNWNSM 250 260 270 280 290 300 TSIADSNDKW ASYAGPGGWN DPDMLEVGNG GMTTEEYRSH FSIWALAKAP LLVGCDIRAM 310 320 330 340 350 360 DDTTHELISN AEVIAVNQDK LGVQGKKVKS TNDLEVWAGP LSDNKVAVIL WNRSSSRATV 370 380 390 400 410 TASWSDIGLQ QGTTVDARDL WEHSTQSLVS GEISAEIDSH ACKMYVLTPR S

References

[1]	"Cloning and nucleotide sequence of the alpha-galactosidase cDNA from Cyamopsis tetragonoloba (guar)." Overbeeke N., Fellinger A.J., Toonen M.Y., van Wassenaar D., Verrips C.T. Plant Mol. Biol. 13:541-550(1989) [PubMed: 2577496] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Aleurone.
[2]	"Messenger RNA from isolated aleurone cells directs the synthesis of an alpha-galactosidase found in the endosperm during germination of guar (Cyamopsis tetragonaloba) seed." Hughes S.G., Overbeeke N., Robinson S., Pollock K., Smeets F.L.M. Plant Mol. Biol. 11:783-789(1988) [Agricola: IND91035194] Cited for: PROTEIN SEQUENCE OF 48-57 AND 172-178. Tissue: Seed.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14619 mRNA. Translation: CAA32772.1.
PIR	S07472.
3D structure databases
ProteinModelPortal	P14749.
SMR	P14749. Positions 49-409.
ModBase	Search...
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAL_CYATE

Accession

Primary (citable) accession number: P14749

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	July 27, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program