Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P14747

- PP2B2_YEAST

UniProt

P14747 - PP2B2_YEAST

Protein

Serine/threonine-protein phosphatase 2B catalytic subunit A2

Gene

CMP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 2 (01 Nov 1991)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 1 Fe3+ ion per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi144 – 1441IronBy similarity
    Metal bindingi146 – 1461IronBy similarity
    Metal bindingi172 – 1721IronBy similarity
    Metal bindingi172 – 1721ZincBy similarity
    Metal bindingi204 – 2041ZincBy similarity
    Active sitei205 – 2051Proton donorBy similarity
    Metal bindingi253 – 2531ZincBy similarity
    Metal bindingi359 – 3591ZincBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. phosphoprotein phosphatase activity Source: UniProtKB-KW
    3. protein binding Source: IntAct

    GO - Biological processi

    1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
    2. cellular ion homeostasis Source: SGD
    3. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Calmodulin-binding, Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32652-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 2B catalytic subunit A2 (EC:3.1.3.16)
    Alternative name(s):
    Calcineurin A2
    Calmodulin-binding protein 2
    Gene namesi
    Name:CMP2
    Synonyms:CNA2
    Ordered Locus Names:YML057W
    ORF Names:YM9958.05
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYML057w.
    SGDiS000004521. CMP2.

    Subcellular locationi

    GO - Cellular componenti

    1. calcineurin complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 604604Serine/threonine-protein phosphatase 2B catalytic subunit A2PRO_0000058837Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei31 – 311Phosphothreonine1 Publication
    Modified residuei489 – 4891Phosphoserine1 Publication
    Modified residuei520 – 5201PhosphoserineSequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP14747.
    PaxDbiP14747.
    PeptideAtlasiP14747.

    Expressioni

    Gene expression databases

    GenevestigatoriP14747.

    Interactioni

    Subunit structurei

    Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CNB1P252963EBI-12778,EBI-3968

    Protein-protein interaction databases

    BioGridi35110. 50 interactions.
    DIPiDIP-833N.
    IntActiP14747. 12 interactions.
    MINTiMINT-519169.
    STRINGi4932.YML057W.

    Structurei

    3D structure databases

    ProteinModelPortaliP14747.
    SMRiP14747. Positions 75-450.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni501 – 52323Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000063087.
    HOGENOMiHOG000172699.
    KOiK04348.
    OMAiTSDYFLH.
    OrthoDBiEOG77M8X9.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 2 hits.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14747-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSDAIRNTE QINAAIKIIE NKTERPQSST TPIDSKASTV AAANSTATET    50
    SRDLTQYTLD DGRVVSTNRR IMNKVPAITS HVPTDEELFQ PNGIPRHEFL 100
    RDHFKREGKL SAAQAARIVT LATELFSKEP NLISVPAPIT VCGDIHGQYF 150
    DLLKLFEVGG DPATTSYLFL GDYVDRGSFS FECLIYLYSL KLNFNDHFWL 200
    LRGNHECKHL TSYFTFKNEM LHKYNLDIYE KCCESFNNLP LAALMNGQYL 250
    CVHGGISPEL NSLQDINNLN RFREIPSHGL MCDLLWADPI EEYDEVLDKD 300
    LTEEDIVNSK TMVPHHGKMA PSRDMFVPNS VRGCSYAFTY RAACHFLQET 350
    GLLSIIRAHE AQDAGYRMYK NTKTLGFPSL LTLFSAPNYL DTYNNKAAIL 400
    KYENNVMNIR QFNMTPHPYW LPDFMDVFTW SLPFVGEKVT EMLVAILNIC 450
    TEDELENDTP VIEELVGTDK KLPQAGKSEA TPQPATSASP KHASILDDEH 500
    RRKALRNKIL AVAKVSRMYS VLREETNKVQ FLKDHNSGVL PRGALSNGVK 550
    GLDEALSTFE RARKHDLINE KLPPSLDELK NENKKYYEKV WQKVHEHDAK 600
    NDSK 604
    Length:604
    Mass (Da):68,528
    Last modified:November 1, 1991 - v2
    Checksum:i4A605598FD04207F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti87 – 882EL → DV in CAA38712. (PubMed:1646387)Curated
    Sequence conflicti581 – 5811N → K in CAA34722. (PubMed:2557079)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64840 Genomic DNA. Translation: AAA34466.1.
    X16804 mRNA. Translation: CAA34722.1.
    Z46729 Genomic DNA. Translation: CAA86718.1.
    X54964 Genomic DNA. Translation: CAA38712.1.
    BK006946 Genomic DNA. Translation: DAA09841.1.
    PIRiS49804.
    RefSeqiNP_013655.1. NM_001182415.1.

    Genome annotation databases

    EnsemblFungiiYML057W; YML057W; YML057W.
    GeneIDi854946.
    KEGGisce:YML057W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64840 Genomic DNA. Translation: AAA34466.1 .
    X16804 mRNA. Translation: CAA34722.1 .
    Z46729 Genomic DNA. Translation: CAA86718.1 .
    X54964 Genomic DNA. Translation: CAA38712.1 .
    BK006946 Genomic DNA. Translation: DAA09841.1 .
    PIRi S49804.
    RefSeqi NP_013655.1. NM_001182415.1.

    3D structure databases

    ProteinModelPortali P14747.
    SMRi P14747. Positions 75-450.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35110. 50 interactions.
    DIPi DIP-833N.
    IntActi P14747. 12 interactions.
    MINTi MINT-519169.
    STRINGi 4932.YML057W.

    Proteomic databases

    MaxQBi P14747.
    PaxDbi P14747.
    PeptideAtlasi P14747.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML057W ; YML057W ; YML057W .
    GeneIDi 854946.
    KEGGi sce:YML057W.

    Organism-specific databases

    CYGDi YML057w.
    SGDi S000004521. CMP2.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000063087.
    HOGENOMi HOG000172699.
    KOi K04348.
    OMAi TSDYFLH.
    OrthoDBi EOG77M8X9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32652-MONOMER.

    Miscellaneous databases

    NextBioi 978007.

    Gene expression databases

    Genevestigatori P14747.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 2 hits.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase."
      Cyert M.S., Kunisawa R., Kaim D., Thorner J.
      Proc. Natl. Acad. Sci. U.S.A. 88:7376-7380(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / GRF88.
    2. "The Saccharomyces cerevisiae genes (CMP1 and CMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B."
      Liu Y., Ishii S., Tokai M., Tsutsumi H., Ohki O., Akada R., Tanaka K., Tsuchiya E., Fukui S., Miyakawa T.
      Mol. Gen. Genet. 227:52-59(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Isolation of a cDNA likely to encode a novel Ca2+-dependent/calmodulin-stimulated protein phosphatase."
      da Cruz e Silva E.F., Cohen P.T.W.
      Biochim. Biophys. Acta 1009:293-296(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-604.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-489, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPP2B2_YEAST
    AccessioniPrimary (citable) accession number: P14747
    Secondary accession number(s): D6VZB7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 144 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7110 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was originally thought to originate from a rabbit cDNA library and was known as protein phosphatase 2Bw (PP2Bw).1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3