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P14747 (PP2B2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit A2

EC=3.1.3.16
Alternative name(s):
Calcineurin A2
Calmodulin-binding protein 2
Gene names
Name:CMP2
Synonyms:CNA2
Ordered Locus Names:YML057W
ORF Names:YM9958.05
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length604 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activity

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactor

Binds 1 Fe3+ ion per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Miscellaneous

Present with 7110 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the PPP phosphatase family. PP-2B subfamily.

Caution

Was originally (Ref.5) thought to originate from a rabbit cDNA library and was known as protein phosphatase 2Bw (PP2Bw).

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CNB1P252963EBI-12778,EBI-3968

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 604604Serine/threonine-protein phosphatase 2B catalytic subunit A2
PRO_0000058837

Regions

Region501 – 52323Calmodulin-binding Potential

Sites

Active site2051Proton donor By similarity
Metal binding1441Iron By similarity
Metal binding1461Iron By similarity
Metal binding1721Iron By similarity
Metal binding1721Zinc By similarity
Metal binding2041Zinc By similarity
Metal binding2531Zinc By similarity
Metal binding3591Zinc By similarity

Amino acid modifications

Modified residue311Phosphothreonine Ref.8
Modified residue4891Phosphoserine Ref.7
Modified residue5201Phosphoserine Potential

Experimental info

Sequence conflict87 – 882EL → DV in CAA38712. Ref.2
Sequence conflict5811N → K in CAA34722. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P14747 [UniParc].

Last modified November 1, 1991. Version 2.
Checksum: 4A605598FD04207F

FASTA60468,528
        10         20         30         40         50         60 
MSSDAIRNTE QINAAIKIIE NKTERPQSST TPIDSKASTV AAANSTATET SRDLTQYTLD 

        70         80         90        100        110        120 
DGRVVSTNRR IMNKVPAITS HVPTDEELFQ PNGIPRHEFL RDHFKREGKL SAAQAARIVT 

       130        140        150        160        170        180 
LATELFSKEP NLISVPAPIT VCGDIHGQYF DLLKLFEVGG DPATTSYLFL GDYVDRGSFS 

       190        200        210        220        230        240 
FECLIYLYSL KLNFNDHFWL LRGNHECKHL TSYFTFKNEM LHKYNLDIYE KCCESFNNLP 

       250        260        270        280        290        300 
LAALMNGQYL CVHGGISPEL NSLQDINNLN RFREIPSHGL MCDLLWADPI EEYDEVLDKD 

       310        320        330        340        350        360 
LTEEDIVNSK TMVPHHGKMA PSRDMFVPNS VRGCSYAFTY RAACHFLQET GLLSIIRAHE 

       370        380        390        400        410        420 
AQDAGYRMYK NTKTLGFPSL LTLFSAPNYL DTYNNKAAIL KYENNVMNIR QFNMTPHPYW 

       430        440        450        460        470        480 
LPDFMDVFTW SLPFVGEKVT EMLVAILNIC TEDELENDTP VIEELVGTDK KLPQAGKSEA 

       490        500        510        520        530        540 
TPQPATSASP KHASILDDEH RRKALRNKIL AVAKVSRMYS VLREETNKVQ FLKDHNSGVL 

       550        560        570        580        590        600 
PRGALSNGVK GLDEALSTFE RARKHDLINE KLPPSLDELK NENKKYYEKV WQKVHEHDAK 


NDSK 

« Hide

References

« Hide 'large scale' references
[1]"Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase."
Cyert M.S., Kunisawa R., Kaim D., Thorner J.
Proc. Natl. Acad. Sci. U.S.A. 88:7376-7380(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: S288c / GRF88.
[2]"The Saccharomyces cerevisiae genes (CMP1 and CMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B."
Liu Y., Ishii S., Tokai M., Tsutsumi H., Ohki O., Akada R., Tanaka K., Tsuchiya E., Fukui S., Miyakawa T.
Mol. Gen. Genet. 227:52-59(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Isolation of a cDNA likely to encode a novel Ca2+-dependent/calmodulin-stimulated protein phosphatase."
da Cruz e Silva E.F., Cohen P.T.W.
Biochim. Biophys. Acta 1009:293-296(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-604.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-489, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64840 Genomic DNA. Translation: AAA34466.1.
X16804 mRNA. Translation: CAA34722.1.
Z46729 Genomic DNA. Translation: CAA86718.1.
X54964 Genomic DNA. Translation: CAA38712.1.
BK006946 Genomic DNA. Translation: DAA09841.1.
PIRS49804.
RefSeqNP_013655.1. NM_001182415.1.

3D structure databases

ProteinModelPortalP14747.
SMRP14747. Positions 75-450.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35110. 50 interactions.
DIPDIP-833N.
IntActP14747. 12 interactions.
MINTMINT-519169.
STRING4932.YML057W.

Proteomic databases

MaxQBP14747.
PaxDbP14747.
PeptideAtlasP14747.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML057W; YML057W; YML057W.
GeneID854946.
KEGGsce:YML057W.

Organism-specific databases

CYGDYML057w.
SGDS000004521. CMP2.

Phylogenomic databases

eggNOGCOG0639.
GeneTreeENSGT00530000063087.
HOGENOMHOG000172699.
KOK04348.
OMATSDYFLH.
OrthoDBEOG77M8X9.

Enzyme and pathway databases

BioCycYEAST:G3O-32652-MONOMER.

Gene expression databases

GenevestigatorP14747.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 2 hits.
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978007.

Entry information

Entry namePP2B2_YEAST
AccessionPrimary (citable) accession number: P14747
Secondary accession number(s): D6VZB7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1991
Last modified: June 11, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families