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Protein

Serine/threonine-protein phosphatase 2B catalytic subunit A2

Gene

CMP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi144 – 1441IronBy similarity
Metal bindingi146 – 1461IronBy similarity
Metal bindingi172 – 1721IronBy similarity
Metal bindingi172 – 1721ZincBy similarity
Metal bindingi204 – 2041ZincBy similarity
Active sitei205 – 2051Proton donorBy similarity
Metal bindingi253 – 2531ZincBy similarity
Metal bindingi359 – 3591ZincBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
  2. cellular ion homeostasis Source: SGD
  3. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32652-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit A2 (EC:3.1.3.16)
Alternative name(s):
Calcineurin A2
Calmodulin-binding protein 2
Gene namesi
Name:CMP2
Synonyms:CNA2
Ordered Locus Names:YML057W
ORF Names:YM9958.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYML057w.
SGDiS000004521. CMP2.

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 604604Serine/threonine-protein phosphatase 2B catalytic subunit A2PRO_0000058837Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei31 – 311Phosphothreonine1 Publication
Modified residuei489 – 4891Phosphoserine1 Publication
Modified residuei520 – 5201PhosphoserineSequence Analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP14747.
PaxDbiP14747.
PeptideAtlasiP14747.

Expressioni

Gene expression databases

GenevestigatoriP14747.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
CNB1P252963EBI-12778,EBI-3968

Protein-protein interaction databases

BioGridi35110. 51 interactions.
DIPiDIP-833N.
IntActiP14747. 12 interactions.
MINTiMINT-519169.
STRINGi4932.YML057W.

Structurei

3D structure databases

ProteinModelPortaliP14747.
SMRiP14747. Positions 75-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni501 – 52323Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
InParanoidiP14747.
KOiK04348.
OMAiHHEQSER.
OrthoDBiEOG77M8X9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14747-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDAIRNTE QINAAIKIIE NKTERPQSST TPIDSKASTV AAANSTATET
60 70 80 90 100
SRDLTQYTLD DGRVVSTNRR IMNKVPAITS HVPTDEELFQ PNGIPRHEFL
110 120 130 140 150
RDHFKREGKL SAAQAARIVT LATELFSKEP NLISVPAPIT VCGDIHGQYF
160 170 180 190 200
DLLKLFEVGG DPATTSYLFL GDYVDRGSFS FECLIYLYSL KLNFNDHFWL
210 220 230 240 250
LRGNHECKHL TSYFTFKNEM LHKYNLDIYE KCCESFNNLP LAALMNGQYL
260 270 280 290 300
CVHGGISPEL NSLQDINNLN RFREIPSHGL MCDLLWADPI EEYDEVLDKD
310 320 330 340 350
LTEEDIVNSK TMVPHHGKMA PSRDMFVPNS VRGCSYAFTY RAACHFLQET
360 370 380 390 400
GLLSIIRAHE AQDAGYRMYK NTKTLGFPSL LTLFSAPNYL DTYNNKAAIL
410 420 430 440 450
KYENNVMNIR QFNMTPHPYW LPDFMDVFTW SLPFVGEKVT EMLVAILNIC
460 470 480 490 500
TEDELENDTP VIEELVGTDK KLPQAGKSEA TPQPATSASP KHASILDDEH
510 520 530 540 550
RRKALRNKIL AVAKVSRMYS VLREETNKVQ FLKDHNSGVL PRGALSNGVK
560 570 580 590 600
GLDEALSTFE RARKHDLINE KLPPSLDELK NENKKYYEKV WQKVHEHDAK

NDSK
Length:604
Mass (Da):68,528
Last modified:October 31, 1991 - v2
Checksum:i4A605598FD04207F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 882EL → DV in CAA38712 (PubMed:1646387).Curated
Sequence conflicti581 – 5811N → K in CAA34722 (PubMed:2557079).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64840 Genomic DNA. Translation: AAA34466.1.
X16804 mRNA. Translation: CAA34722.1.
Z46729 Genomic DNA. Translation: CAA86718.1.
X54964 Genomic DNA. Translation: CAA38712.1.
BK006946 Genomic DNA. Translation: DAA09841.1.
PIRiS49804.
RefSeqiNP_013655.1. NM_001182415.1.

Genome annotation databases

EnsemblFungiiYML057W; YML057W; YML057W.
GeneIDi854946.
KEGGisce:YML057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64840 Genomic DNA. Translation: AAA34466.1.
X16804 mRNA. Translation: CAA34722.1.
Z46729 Genomic DNA. Translation: CAA86718.1.
X54964 Genomic DNA. Translation: CAA38712.1.
BK006946 Genomic DNA. Translation: DAA09841.1.
PIRiS49804.
RefSeqiNP_013655.1. NM_001182415.1.

3D structure databases

ProteinModelPortaliP14747.
SMRiP14747. Positions 75-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35110. 51 interactions.
DIPiDIP-833N.
IntActiP14747. 12 interactions.
MINTiMINT-519169.
STRINGi4932.YML057W.

Proteomic databases

MaxQBiP14747.
PaxDbiP14747.
PeptideAtlasiP14747.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML057W; YML057W; YML057W.
GeneIDi854946.
KEGGisce:YML057W.

Organism-specific databases

CYGDiYML057w.
SGDiS000004521. CMP2.

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
InParanoidiP14747.
KOiK04348.
OMAiHHEQSER.
OrthoDBiEOG77M8X9.

Enzyme and pathway databases

BioCyciYEAST:G3O-32652-MONOMER.

Miscellaneous databases

NextBioi978007.

Gene expression databases

GenevestigatoriP14747.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase."
    Cyert M.S., Kunisawa R., Kaim D., Thorner J.
    Proc. Natl. Acad. Sci. U.S.A. 88:7376-7380(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "The Saccharomyces cerevisiae genes (CMP1 and CMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B."
    Liu Y., Ishii S., Tokai M., Tsutsumi H., Ohki O., Akada R., Tanaka K., Tsuchiya E., Fukui S., Miyakawa T.
    Mol. Gen. Genet. 227:52-59(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Isolation of a cDNA likely to encode a novel Ca2+-dependent/calmodulin-stimulated protein phosphatase."
    da Cruz e Silva E.F., Cohen P.T.W.
    Biochim. Biophys. Acta 1009:293-296(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 100-604.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-489, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-31, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP2B2_YEAST
AccessioniPrimary (citable) accession number: P14747
Secondary accession number(s): D6VZB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 31, 1990
Last sequence update: October 31, 1991
Last modified: January 6, 2015
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7110 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to originate from a rabbit cDNA library and was known as protein phosphatase 2Bw (PP2Bw).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.