Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase 2B catalytic subunit A2

Gene

CMP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144IronBy similarity1
Metal bindingi146IronBy similarity1
Metal bindingi172IronBy similarity1
Metal bindingi172ZincBy similarity1
Metal bindingi204ZincBy similarity1
Active sitei205Proton donorBy similarity1
Metal bindingi253ZincBy similarity1
Metal bindingi359ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
  • cellular ion homeostasis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32652-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit A2 (EC:3.1.3.16)
Alternative name(s):
Calcineurin A2
Calmodulin-binding protein 2
Gene namesi
Name:CMP2
Synonyms:CNA2
Ordered Locus Names:YML057W
ORF Names:YM9958.05
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML057W.
SGDiS000004521. CMP2.

Subcellular locationi

GO - Cellular componenti

  • calcineurin complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588371 – 604Serine/threonine-protein phosphatase 2B catalytic subunit A2Add BLAST604

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31PhosphothreonineCombined sources1
Modified residuei489PhosphoserineCombined sources1
Modified residuei520PhosphoserineSequence analysis1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP14747.
PRIDEiP14747.
TopDownProteomicsiP14747.

PTM databases

iPTMnetiP14747.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
CNB1P252963EBI-12778,EBI-3968

Protein-protein interaction databases

BioGridi35110. 51 interactors.
DIPiDIP-833N.
IntActiP14747. 12 interactors.
MINTiMINT-519169.

Structurei

3D structure databases

ProteinModelPortaliP14747.
SMRiP14747.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni501 – 523Calmodulin-bindingSequence analysisAdd BLAST23

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
InParanoidiP14747.
KOiK04348.
OMAiENSERVM.
OrthoDBiEOG092C1CSB.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14747-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDAIRNTE QINAAIKIIE NKTERPQSST TPIDSKASTV AAANSTATET
60 70 80 90 100
SRDLTQYTLD DGRVVSTNRR IMNKVPAITS HVPTDEELFQ PNGIPRHEFL
110 120 130 140 150
RDHFKREGKL SAAQAARIVT LATELFSKEP NLISVPAPIT VCGDIHGQYF
160 170 180 190 200
DLLKLFEVGG DPATTSYLFL GDYVDRGSFS FECLIYLYSL KLNFNDHFWL
210 220 230 240 250
LRGNHECKHL TSYFTFKNEM LHKYNLDIYE KCCESFNNLP LAALMNGQYL
260 270 280 290 300
CVHGGISPEL NSLQDINNLN RFREIPSHGL MCDLLWADPI EEYDEVLDKD
310 320 330 340 350
LTEEDIVNSK TMVPHHGKMA PSRDMFVPNS VRGCSYAFTY RAACHFLQET
360 370 380 390 400
GLLSIIRAHE AQDAGYRMYK NTKTLGFPSL LTLFSAPNYL DTYNNKAAIL
410 420 430 440 450
KYENNVMNIR QFNMTPHPYW LPDFMDVFTW SLPFVGEKVT EMLVAILNIC
460 470 480 490 500
TEDELENDTP VIEELVGTDK KLPQAGKSEA TPQPATSASP KHASILDDEH
510 520 530 540 550
RRKALRNKIL AVAKVSRMYS VLREETNKVQ FLKDHNSGVL PRGALSNGVK
560 570 580 590 600
GLDEALSTFE RARKHDLINE KLPPSLDELK NENKKYYEKV WQKVHEHDAK

NDSK
Length:604
Mass (Da):68,528
Last modified:November 1, 1991 - v2
Checksum:i4A605598FD04207F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87 – 88EL → DV in CAA38712 (PubMed:1646387).Curated2
Sequence conflicti581N → K in CAA34722 (PubMed:2557079).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64840 Genomic DNA. Translation: AAA34466.1.
X16804 mRNA. Translation: CAA34722.1.
Z46729 Genomic DNA. Translation: CAA86718.1.
X54964 Genomic DNA. Translation: CAA38712.1.
BK006946 Genomic DNA. Translation: DAA09841.1.
PIRiS49804.
RefSeqiNP_013655.1. NM_001182415.1.

Genome annotation databases

EnsemblFungiiYML057W; YML057W; YML057W.
GeneIDi854946.
KEGGisce:YML057W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64840 Genomic DNA. Translation: AAA34466.1.
X16804 mRNA. Translation: CAA34722.1.
Z46729 Genomic DNA. Translation: CAA86718.1.
X54964 Genomic DNA. Translation: CAA38712.1.
BK006946 Genomic DNA. Translation: DAA09841.1.
PIRiS49804.
RefSeqiNP_013655.1. NM_001182415.1.

3D structure databases

ProteinModelPortaliP14747.
SMRiP14747.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35110. 51 interactors.
DIPiDIP-833N.
IntActiP14747. 12 interactors.
MINTiMINT-519169.

PTM databases

iPTMnetiP14747.

Proteomic databases

MaxQBiP14747.
PRIDEiP14747.
TopDownProteomicsiP14747.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML057W; YML057W; YML057W.
GeneIDi854946.
KEGGisce:YML057W.

Organism-specific databases

EuPathDBiFungiDB:YML057W.
SGDiS000004521. CMP2.

Phylogenomic databases

GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
InParanoidiP14747.
KOiK04348.
OMAiENSERVM.
OrthoDBiEOG092C1CSB.

Enzyme and pathway databases

BioCyciYEAST:G3O-32652-MONOMER.

Miscellaneous databases

PROiP14747.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP2B2_YEAST
AccessioniPrimary (citable) accession number: P14747
Secondary accession number(s): D6VZB7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 1, 1991
Last modified: November 30, 2016
This is version 165 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7110 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to originate from a rabbit cDNA library and was known as protein phosphatase 2Bw (PP2Bw).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.