P14746 (REC1_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA repair exonuclease REC1
EC=3.1.11.2

Gene names

Name:	REC1
ORF Names:	UM11016, UM03380

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a central role in regulating the genetic system of this fungus. Has a 3'-->5' exonuclease activity. Ref.2
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Subcellular location	Nucleus Potential.
Disruption phenotype	Cells are very sensitive to UV light. Ref.2
Sequence similarities	Belongs to the rad1 family.
Sequence caution	The sequence CAA35660.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EAK84248.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Exonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	damaged DNA binding Inferred from electronic annotation. Source: InterPro exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	DNA repair exonuclease REC1		PRO_0000097223
Regions
	Compositional bias	424 – 429	6	Poly-Ala
Experimental info
	Sequence conflict	334	1	N → K in CAA35660. Ref.1
	Sequence conflict	334	1	N → K in AAB60310. Ref.2
	Sequence conflict	354	1	E → Q in CAA35660. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P14746 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: B509C9090159F61A	FASTA	463	50,127
	10 20 30 40 50 60 MPAEGACDAA SLMTLTATLS DVTGLANLLK SVAIQTHAVV IASSSGLEII TELNRTLQAH 70 80 90 100 110 120 AYLYSHMFDS YRFENAQDDV RGSTSLQARS RPKKRSKLTS KHAETADSQS SAASSDHESG 130 140 150 160 170 180 QSQAHTKKRF RANSHSYAQE ADRVHDEPDS VSFEVNLQTW ISCLNIFGGV GPSRPHSSSS 190 200 210 220 230 240 GLPGFRPEQG SAEAPPGGRG YQRTRYGVAD AYGAERGTSV ERGFDRNPFS SSAKATRMKL 250 260 270 280 290 300 SYQGHGNPLV LELEQDANVL TRVSMSTYEP SFLTDMVFEP QNMVAQVIVA SELMQSAFTE 310 320 330 340 350 360 IDASCKKLSI LITSPHSLST YDGDQRTEAP APTNRNTSAS MLKFRAISDT GSSEMEFPAS 370 380 390 400 410 420 LTSSDPTGVI EKFVALPGSS EQWYDFTLLS RTMSVLRSSI KTSLRMDEAG LISFQFMMPK 430 440 450 460 YRRAAAAGAP LTNAAAGQAA HEDEQDAFCE FLCCPLDTST LIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the REC1 gene of Ustilago maydis." Holden D.W., Spanos A., Banks G.R. Nucleic Acids Res. 17:10489-10489(1989) [PubMed: 2602156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The REC1 gene of Ustilago maydis involved in the cellular response to DNA damage encodes an exonuclease." Thelen M.P., Onel K., Holloman W.K. J. Biol. Chem. 269:747-754(1994) [PubMed: 8276878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
[3]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.
[4]	"Mutation avoidance and DNA repair proficiency in Ustilago maydis are differentially lost with progressive truncation of the REC1 gene product." Onel K., Thelen M.P., Ferguson D.O., Bennett R.L., Holloman W.K. Mol. Cell. Biol. 15:5329-5338(1995) [PubMed: 7565682] [Abstract] Cited for: IDENTIFICATION OF INTRON.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17663 Genomic DNA. Translation: CAA35660.1. Sequence problems. U01836 Genomic DNA. Translation: AAB60310.1. AACP01000114 Genomic DNA. Translation: EAK84248.1. Sequence problems.
PIR	RGUS1M. S06912.
RefSeq	XP_759527.1. XM_754434.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3631467.
KEGG	uma:UM03380.1.
Phylogenomic databases
eggNOG	KOG3194.
OrthoDB	EOG405W98.
Family and domain databases
InterPro	IPR003021. Rad1_Rec1. [Graphical view]
KO	K02830.
Pfam	PF02144. Rad1. 1 hit. [Graphical view]
PRINTS	PR01245. RAD1REC1.
ProtoNet	Search...

Entry information

Entry name

REC1_USTMA

Accession

Primary (citable) accession number: P14746
Secondary accession number(s): Q4P933, Q99110

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	December 12, 2006
Last modified:	November 16, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program