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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

GFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).1 Publication

Miscellaneous

Present with 14300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.1 Publication

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative glutamine--fructose-6-phosphate aminotransferase [isomerizing] (YMR084W), Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (GFA1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activity1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YKL104C-MONOMER
ReactomeiR-SCE-446210 Synthesis of UDP-N-acetyl-glucosamine
UniPathwayiUPA00113; UER00528

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
Short name:
GFAT
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene namesi
Name:GFA1
Ordered Locus Names:YKL104C
ORF Names:YKL457
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL104C
SGDiS000001587 GFA1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001352892 – 717Glutamine--fructose-6-phosphate aminotransferase [isomerizing]Add BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253PhosphoserineCombined sources1
Modified residuei334PhosphothreonineCombined sources1
Modified residuei336PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP14742
PaxDbiP14742
PRIDEiP14742

PTM databases

iPTMnetiP14742

Expressioni

Inductioni

The activity of this enzyme increases in presence of mating pheromone (transcriptional regulation).

Interactioni

Protein-protein interaction databases

BioGridi34030, 197 interactors
DIPiDIP-5224N
IntActiP14742, 144 interactors
MINTiP14742
STRINGi4932.YKL104C

Structurei

3D structure databases

ProteinModelPortaliP14742
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 318Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST317
Domaini390 – 529SIS 1PROSITE-ProRule annotationAdd BLAST140
Domaini562 – 707SIS 2PROSITE-ProRule annotationAdd BLAST146

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

GeneTreeiENSGT00390000010049
HOGENOMiHOG000258898
InParanoidiP14742
KOiK00820
OMAiASEYRYA
OrthoDBiEOG092C0ST1

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 2 hits
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14742-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFGYCNY LVERSRGEII DTLVDGLQRL EYRGYDSTGI AIDGDEADST
60 70 80 90 100
FIYKQIGKVS ALKEEITKQN PNRDVTFVSH CGIAHTRWAT HGRPEQVNCH
110 120 130 140 150
PQRSDPEDQF VVVHNGIITN FRELKTLLIN KGYKFESDTD TECIAKLYLH
160 170 180 190 200
LYNTNLQNGH DLDFHELTKL VLLELEGSYG LLCKSCHYPN EVIATRKGSP
210 220 230 240 250
LLIGVKSEKK LKVDFVDVEF PEENAGQPEI PLKSNNKSFG LGPKKAREFE
260 270 280 290 300
AGSQNANLLP IAANEFNLRH SQSRAFLSED GSPTPVEFFV SSDAASVVKH
310 320 330 340 350
TKKVLFLEDD DLAHIYDGEL HIHRSRREVG ASMTRSIQTL EMELAQIMKG
360 370 380 390 400
PYDHFMQKEI YEQPESTFNT MRGRIDYENN KVILGGLKAW LPVVRRARRL
410 420 430 440 450
IMIACGTSYH SCLATRAIFE ELSDIPVSVE LASDFLDRKC PVFRDDVCVF
460 470 480 490 500
VSQSGETADT MLALNYCLER GALTVGIVNS VGSSISRVTH CGVHINAGPE
510 520 530 540 550
IGVASTKAYT SQYIALVMFA LSLSDDRVSK IDRRIEIIQG LKLIPGQIKQ
560 570 580 590 600
VLKLEPRIKK LCATELKDQK SLLLLGRGYQ FAAALEGALK IKEISYMHSE
610 620 630 640 650
GVLAGELKHG VLALVDENLP IIAFGTRDSL FPKVVSSIEQ VTARKGHPII
660 670 680 690 700
ICNENDEVWA QKSKSIDLQT LEVPQTVDCL QGLINIIPLQ LMSYWLAVNK
710
GIDVDFPRNL AKSVTVE
Length:717
Mass (Da):80,047
Last modified:January 23, 2007 - v4
Checksum:i27E5F0D24BDC451A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti453 – 454QS → HC in AAA34643 (PubMed:2656689).Curated2
Sequence conflicti534Missing in AAA34643 (PubMed:2656689).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04719 Genomic DNA Translation: AAA34643.1
X71133 Genomic DNA Translation: CAA50453.1
Z28104 Genomic DNA Translation: CAA81944.1
BK006944 Genomic DNA Translation: DAA09054.1
PIRiS37931 XNBYGM
RefSeqiNP_012818.1, NM_001179670.1

Genome annotation databases

EnsemblFungiiYKL104C; YKL104C; YKL104C
GeneIDi853757
KEGGisce:YKL104C

Similar proteinsi

Entry informationi

Entry nameiGFA1_YEAST
AccessioniPrimary (citable) accession number: P14742
Secondary accession number(s): D6VXI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 174 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

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