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P14742 (GFA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Short name=GFAT
EC=2.6.1.16
Alternative name(s):
D-fructose-6-phosphate amidotransferase
Hexosephosphate aminotransferase
Gene names
Name:GFA1
Ordered Locus Names:YKL104C
ORF Names:YKL457
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length717 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins). Ref.6

Catalytic activity

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate. Ref.6

Pathway

Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate: step 1/1.

Induction

The activity of this enzyme increases in presence of mating pheromone (transcriptional regulation).

Miscellaneous

Present with 14300 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 glutamine amidotransferase type-2 domain.

Contains 2 SIS domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRR1P287081EBI-7557,EBI-9782

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.6
Chain2 – 717716Glutamine--fructose-6-phosphate aminotransferase [isomerizing]
PRO_0000135289

Regions

Domain2 – 318317Glutamine amidotransferase type-2
Domain390 – 529140SIS 1
Domain562 – 707146SIS 2

Sites

Active site21For GATase activity Ref.6

Amino acid modifications

Modified residue2531Phosphoserine Ref.7 Ref.8
Modified residue3341Phosphothreonine Ref.7
Modified residue3361Phosphoserine Ref.8

Experimental info

Sequence conflict453 – 4542QS → HC in AAA34643. Ref.1
Sequence conflict5341Missing in AAA34643. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P14742 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 27E5F0D24BDC451A

FASTA71780,047
        10         20         30         40         50         60 
MCGIFGYCNY LVERSRGEII DTLVDGLQRL EYRGYDSTGI AIDGDEADST FIYKQIGKVS 

        70         80         90        100        110        120 
ALKEEITKQN PNRDVTFVSH CGIAHTRWAT HGRPEQVNCH PQRSDPEDQF VVVHNGIITN 

       130        140        150        160        170        180 
FRELKTLLIN KGYKFESDTD TECIAKLYLH LYNTNLQNGH DLDFHELTKL VLLELEGSYG 

       190        200        210        220        230        240 
LLCKSCHYPN EVIATRKGSP LLIGVKSEKK LKVDFVDVEF PEENAGQPEI PLKSNNKSFG 

       250        260        270        280        290        300 
LGPKKAREFE AGSQNANLLP IAANEFNLRH SQSRAFLSED GSPTPVEFFV SSDAASVVKH 

       310        320        330        340        350        360 
TKKVLFLEDD DLAHIYDGEL HIHRSRREVG ASMTRSIQTL EMELAQIMKG PYDHFMQKEI 

       370        380        390        400        410        420 
YEQPESTFNT MRGRIDYENN KVILGGLKAW LPVVRRARRL IMIACGTSYH SCLATRAIFE 

       430        440        450        460        470        480 
ELSDIPVSVE LASDFLDRKC PVFRDDVCVF VSQSGETADT MLALNYCLER GALTVGIVNS 

       490        500        510        520        530        540 
VGSSISRVTH CGVHINAGPE IGVASTKAYT SQYIALVMFA LSLSDDRVSK IDRRIEIIQG 

       550        560        570        580        590        600 
LKLIPGQIKQ VLKLEPRIKK LCATELKDQK SLLLLGRGYQ FAAALEGALK IKEISYMHSE 

       610        620        630        640        650        660 
GVLAGELKHG VLALVDENLP IIAFGTRDSL FPKVVSSIEQ VTARKGHPII ICNENDEVWA 

       670        680        690        700        710 
QKSKSIDLQT LEVPQTVDCL QGLINIIPLQ LMSYWLAVNK GIDVDFPRNL AKSVTVE 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of the glutamine:fructose-6-phosphate amidotransferase gene from yeast. Pheromonal regulation of its transcription."
Watzele G., Tanner W.
J. Biol. Chem. 264:8753-8758(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 26786 / X2180-1A.
[2]"The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast."
Cheret G., Pallier C., Valens M., Daignan-Fornier B., Fukuhara H., Bolotin-Fukuhara M., Sor F.
Yeast 9:1259-1265(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Yeast glutamine-fructose-6-phosphate aminotransferase (Gfa1) requires methionine aminopeptidase activity for proper function."
Dummitt B., Micka W.S., Chang Y.H.
J. Biol. Chem. 280:14356-14360(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACTIVE SITE, FUNCTION, CATALYTIC ACTIVITY.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-253 AND THR-334, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-253 AND SER-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04719 Genomic DNA. Translation: AAA34643.1.
X71133 Genomic DNA. Translation: CAA50453.1.
Z28104 Genomic DNA. Translation: CAA81944.1.
BK006944 Genomic DNA. Translation: DAA09054.1.
PIRXNBYGM. S37931.
RefSeqNP_012818.1. NM_001179670.1.

3D structure databases

ProteinModelPortalP14742.
SMRP14742. Positions 2-717.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34030. 94 interactions.
DIPDIP-5224N.
IntActP14742. 119 interactions.
MINTMINT-566761.
STRING4932.YKL104C.

Chemistry

BindingDBP14742.

Proteomic databases

PaxDbP14742.
PeptideAtlasP14742.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL104C; YKL104C; YKL104C.
GeneID853757.
KEGGsce:YKL104C.

Organism-specific databases

SGDS000001587. GFA1.

Phylogenomic databases

eggNOGCOG0449.
GeneTreeENSGT00390000010049.
HOGENOMHOG000258898.
KOK00820.
OMADGNNHEV.
OrthoDBEOG7034RG.

Enzyme and pathway databases

BioCycYEAST:YKL104C-MONOMER.
UniPathwayUPA00113; UER00528.

Gene expression databases

GenevestigatorP14742.

Family and domain databases

InterProIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR001347. SIS.
[Graphical view]
PANTHERPTHR10937:SF0. PTHR10937:SF0. 1 hit.
PfamPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio974834.
PROP14742.

Entry information

Entry nameGFA1_YEAST
AccessionPrimary (citable) accession number: P14742
Secondary accession number(s): D6VXI4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 136 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways