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P14742

- GFA1_YEAST

UniProt

P14742 - GFA1_YEAST

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing]

Gene

GFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in amino sugar synthesis (formation of chitin, supplies the amino sugars of asparagine-linked oligosaccharides of glycoproteins).1 Publication

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activity1 Publication

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: SGD

    GO - Biological processi

    1. fungal-type cell wall chitin biosynthetic process Source: SGD
    2. glutamine metabolic process Source: UniProtKB-KW
    3. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Enzyme and pathway databases

    BioCyciYEAST:YKL104C-MONOMER.
    ReactomeiREACT_188969. XBP1(S) activates chaperone genes.
    REACT_189213. Synthesis of UDP-N-acetyl-glucosamine.
    UniPathwayiUPA00113; UER00528.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (EC:2.6.1.16)
    Short name:
    GFAT
    Alternative name(s):
    D-fructose-6-phosphate amidotransferase
    Hexosephosphate aminotransferase
    Gene namesi
    Name:GFA1
    Ordered Locus Names:YKL104C
    ORF Names:YKL457
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    SGDiS000001587. GFA1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 717716Glutamine--fructose-6-phosphate aminotransferase [isomerizing]PRO_0000135289Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei253 – 2531Phosphoserine2 Publications
    Modified residuei334 – 3341Phosphothreonine1 Publication
    Modified residuei336 – 3361Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP14742.
    PaxDbiP14742.
    PeptideAtlasiP14742.

    Expressioni

    Inductioni

    The activity of this enzyme increases in presence of mating pheromone (transcriptional regulation).

    Gene expression databases

    GenevestigatoriP14742.

    Interactioni

    Protein-protein interaction databases

    BioGridi34030. 94 interactions.
    DIPiDIP-5224N.
    IntActiP14742. 119 interactions.
    MINTiMINT-566761.
    STRINGi4932.YKL104C.

    Structurei

    3D structure databases

    ProteinModelPortaliP14742.
    SMRiP14742. Positions 2-205, 278-717.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 318317Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini390 – 529140SIS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini562 – 707146SIS 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
    Contains 2 SIS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    GeneTreeiENSGT00390000010049.
    HOGENOMiHOG000258898.
    KOiK00820.
    OMAiFYTHAGP.
    OrthoDBiEOG7034RG.

    Family and domain databases

    Gene3Di3.60.20.10. 2 hits.
    InterProiIPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PANTHERiPTHR10937:SF0. PTHR10937:SF0. 1 hit.
    PfamiPF00310. GATase_2. 2 hits.
    PF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 2 hits.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P14742-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIFGYCNY LVERSRGEII DTLVDGLQRL EYRGYDSTGI AIDGDEADST    50
    FIYKQIGKVS ALKEEITKQN PNRDVTFVSH CGIAHTRWAT HGRPEQVNCH 100
    PQRSDPEDQF VVVHNGIITN FRELKTLLIN KGYKFESDTD TECIAKLYLH 150
    LYNTNLQNGH DLDFHELTKL VLLELEGSYG LLCKSCHYPN EVIATRKGSP 200
    LLIGVKSEKK LKVDFVDVEF PEENAGQPEI PLKSNNKSFG LGPKKAREFE 250
    AGSQNANLLP IAANEFNLRH SQSRAFLSED GSPTPVEFFV SSDAASVVKH 300
    TKKVLFLEDD DLAHIYDGEL HIHRSRREVG ASMTRSIQTL EMELAQIMKG 350
    PYDHFMQKEI YEQPESTFNT MRGRIDYENN KVILGGLKAW LPVVRRARRL 400
    IMIACGTSYH SCLATRAIFE ELSDIPVSVE LASDFLDRKC PVFRDDVCVF 450
    VSQSGETADT MLALNYCLER GALTVGIVNS VGSSISRVTH CGVHINAGPE 500
    IGVASTKAYT SQYIALVMFA LSLSDDRVSK IDRRIEIIQG LKLIPGQIKQ 550
    VLKLEPRIKK LCATELKDQK SLLLLGRGYQ FAAALEGALK IKEISYMHSE 600
    GVLAGELKHG VLALVDENLP IIAFGTRDSL FPKVVSSIEQ VTARKGHPII 650
    ICNENDEVWA QKSKSIDLQT LEVPQTVDCL QGLINIIPLQ LMSYWLAVNK 700
    GIDVDFPRNL AKSVTVE 717
    Length:717
    Mass (Da):80,047
    Last modified:January 23, 2007 - v4
    Checksum:i27E5F0D24BDC451A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti453 – 4542QS → HC in AAA34643. (PubMed:2656689)Curated
    Sequence conflicti534 – 5341Missing in AAA34643. (PubMed:2656689)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04719 Genomic DNA. Translation: AAA34643.1.
    X71133 Genomic DNA. Translation: CAA50453.1.
    Z28104 Genomic DNA. Translation: CAA81944.1.
    BK006944 Genomic DNA. Translation: DAA09054.1.
    PIRiS37931. XNBYGM.
    RefSeqiNP_012818.1. NM_001179670.1.

    Genome annotation databases

    EnsemblFungiiYKL104C; YKL104C; YKL104C.
    GeneIDi853757.
    KEGGisce:YKL104C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04719 Genomic DNA. Translation: AAA34643.1 .
    X71133 Genomic DNA. Translation: CAA50453.1 .
    Z28104 Genomic DNA. Translation: CAA81944.1 .
    BK006944 Genomic DNA. Translation: DAA09054.1 .
    PIRi S37931. XNBYGM.
    RefSeqi NP_012818.1. NM_001179670.1.

    3D structure databases

    ProteinModelPortali P14742.
    SMRi P14742. Positions 2-205, 278-717.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34030. 94 interactions.
    DIPi DIP-5224N.
    IntActi P14742. 119 interactions.
    MINTi MINT-566761.
    STRINGi 4932.YKL104C.

    Chemistry

    BindingDBi P14742.

    Proteomic databases

    MaxQBi P14742.
    PaxDbi P14742.
    PeptideAtlasi P14742.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL104C ; YKL104C ; YKL104C .
    GeneIDi 853757.
    KEGGi sce:YKL104C.

    Organism-specific databases

    SGDi S000001587. GFA1.

    Phylogenomic databases

    eggNOGi COG0449.
    GeneTreei ENSGT00390000010049.
    HOGENOMi HOG000258898.
    KOi K00820.
    OMAi FYTHAGP.
    OrthoDBi EOG7034RG.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00528 .
    BioCyci YEAST:YKL104C-MONOMER.
    Reactomei REACT_188969. XBP1(S) activates chaperone genes.
    REACT_189213. Synthesis of UDP-N-acetyl-glucosamine.

    Miscellaneous databases

    NextBioi 974834.
    PROi P14742.

    Gene expression databases

    Genevestigatori P14742.

    Family and domain databases

    Gene3Di 3.60.20.10. 2 hits.
    InterProi IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    PANTHERi PTHR10937:SF0. PTHR10937:SF0. 1 hit.
    Pfami PF00310. GATase_2. 2 hits.
    PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 2 hits.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the glutamine:fructose-6-phosphate amidotransferase gene from yeast. Pheromonal regulation of its transcription."
      Watzele G., Tanner W.
      J. Biol. Chem. 264:8753-8758(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 26786 / X2180-1A.
    2. "The DNA sequence analysis of the HAP4-LAP4 region on chromosome XI of Saccharomyces cerevisiae suggests the presence of a second aspartate aminotransferase gene in yeast."
      Cheret G., Pallier C., Valens M., Daignan-Fornier B., Fukuhara H., Bolotin-Fukuhara M., Sor F.
      Yeast 9:1259-1265(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Yeast glutamine-fructose-6-phosphate aminotransferase (Gfa1) requires methionine aminopeptidase activity for proper function."
      Dummitt B., Micka W.S., Chang Y.H.
      J. Biol. Chem. 280:14356-14360(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CLEAVAGE OF INITIATOR METHIONINE, ACTIVE SITE, FUNCTION, CATALYTIC ACTIVITY.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-253 AND THR-334, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-253 AND SER-336, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGFA1_YEAST
    AccessioniPrimary (citable) accession number: P14742
    Secondary accession number(s): D6VXI4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 141 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 14300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3