Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Translation initiation factor eIF-2B subunit alpha

Gene

GCN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a non-essential regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. Modulation of GCN3 regulatory function in response to amino acid availability occurs post-translationally.2 Publications

GO - Molecular functioni

  • enzyme regulator activity Source: SGD
  • identical protein binding Source: IntAct
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • positive regulation of cellular response to amino acid starvation Source: SGD
  • positive regulation of GTPase activity Source: GOC
  • regulation of catalytic activity Source: GOC
  • regulation of translational initiation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator, Initiation factor, Repressor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32002-MONOMER.
ReactomeiREACT_334024. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor eIF-2B subunit alpha
Alternative name(s):
GCD complex subunit GCN3
Guanine nucleotide exchange factor subunit GCN3
Transcriptional activator GCN3
eIF-2B GDP-GTP exchange factor subunit alpha
Gene namesi
Name:GCN3
Synonyms:AAS2, TIF221
Ordered Locus Names:YKR026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XI

Organism-specific databases

CYGDiYKR026c.
EuPathDBiFungiDB:YKR026C.
SGDiS000001734. GCN3.

Subcellular locationi

GO - Cellular componenti

  • eukaryotic translation initiation factor 2B complex Source: SGD
  • guanyl-nucleotide exchange factor complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 305304Translation initiation factor eIF-2B subunit alphaPRO_0000156060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei291 – 2911Phosphothreonine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP14741.
PaxDbiP14741.
PeptideAtlasiP14741.

Interactioni

Subunit structurei

Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-6253,EBI-6253
BIT2P383463EBI-6253,EBI-21144
GCD2P127543EBI-6253,EBI-6265
GCD7P325027EBI-6253,EBI-6260

Protein-protein interaction databases

BioGridi34158. 94 interactions.
DIPiDIP-1328N.
IntActiP14741. 27 interactions.
MINTiMINT-385513.

Structurei

3D structure databases

ProteinModelPortaliP14741.
SMRiP14741. Positions 1-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1184.
GeneTreeiENSGT00550000074853.
HOGENOMiHOG000175731.
InParanoidiP14741.
KOiK03239.
OMAiHAMARTS.
OrthoDBiEOG73BVPK.

Family and domain databases

InterProiIPR000649. IF-2B-related.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14741-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFNITETY LRFLEEDTEM TMPIAAIEAL VTLLRIKTPE TAAEMINTIK
60 70 80 90 100
SSTEELIKSI PNSVSLRAGC DIFMRFVLRN LHLYGDWENC KQHLIENGQL
110 120 130 140 150
FVSRAKKSRN KIAEIGVDFI ADDDIILVHG YSRAVFSLLN HAANKFIRFR
160 170 180 190 200
CVVTESRPSK QGNQLYTLLE QKGIPVTLIV DSAVGAVIDK VDKVFVGAEG
210 220 230 240 250
VAESGGIINL VGTYSVGVLA HNARKPFYVV TESHKFVRMF PLSSDDLPMA
260 270 280 290 300
GPPLDFTRRT DDLEDALRGP TIDYTAQEYI TALITDLGVL TPSAVSEELI

KMWYD
Length:305
Mass (Da):34,025
Last modified:April 1, 1990 - v1
Checksum:i8E9A928D6D56E12B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23356 Genomic DNA. Translation: AAA34637.1.
Z28251 Genomic DNA. Translation: CAA82098.1.
BK006944 Genomic DNA. Translation: DAA09181.1.
PIRiA31562.
RefSeqiNP_012951.1. NM_001179816.1.

Genome annotation databases

EnsemblFungiiYKR026C; YKR026C; YKR026C.
GeneIDi853896.
KEGGisce:YKR026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23356 Genomic DNA. Translation: AAA34637.1.
Z28251 Genomic DNA. Translation: CAA82098.1.
BK006944 Genomic DNA. Translation: DAA09181.1.
PIRiA31562.
RefSeqiNP_012951.1. NM_001179816.1.

3D structure databases

ProteinModelPortaliP14741.
SMRiP14741. Positions 1-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34158. 94 interactions.
DIPiDIP-1328N.
IntActiP14741. 27 interactions.
MINTiMINT-385513.

Proteomic databases

MaxQBiP14741.
PaxDbiP14741.
PeptideAtlasiP14741.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKR026C; YKR026C; YKR026C.
GeneIDi853896.
KEGGisce:YKR026C.

Organism-specific databases

CYGDiYKR026c.
EuPathDBiFungiDB:YKR026C.
SGDiS000001734. GCN3.

Phylogenomic databases

eggNOGiCOG1184.
GeneTreeiENSGT00550000074853.
HOGENOMiHOG000175731.
InParanoidiP14741.
KOiK03239.
OMAiHAMARTS.
OrthoDBiEOG73BVPK.

Enzyme and pathway databases

BioCyciYEAST:G3O-32002-MONOMER.
ReactomeiREACT_334024. Recycling of eIF2:GDP.

Miscellaneous databases

NextBioi975213.
PROiP14741.

Family and domain databases

InterProiIPR000649. IF-2B-related.
[Graphical view]
PfamiPF01008. IF-2B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of GCN3, a translational activator of GCN4: evidence for posttranslational control of GCN3 regulatory function."
    Hannig E.M., Hinnebusch A.G.
    Mol. Cell. Biol. 8:4808-4820(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast."
    Cigan A.M., Bushman J.L., Boal T.R., Hinnebusch A.G.
    Proc. Natl. Acad. Sci. U.S.A. 90:5350-5354(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE EIF2-B COMPLEX, FUNCTION OF THE EIF2-B COMPLEX.
  5. "eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange."
    Pavitt G.D., Ramaiah K.V., Kimball S.R., Hinnebusch A.G.
    Genes Dev. 12:514-526(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN A EIF2-B SUBCOMPLEX.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-291, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiEI2BA_YEAST
AccessioniPrimary (citable) accession number: P14741
Secondary accession number(s): D6VX91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 22, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.