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Protein

Translation initiation factor eIF-2B subunit alpha

Gene

GCN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a non-essential regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. Modulation of GCN3 regulatory function in response to amino acid availability occurs post-translationally.2 Publications

Miscellaneous

Present with 8970 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • enzyme regulator activity Source: SGD
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • positive regulation of cellular response to amino acid starvation Source: SGD
  • regulation of translational initiation Source: SGD

Keywordsi

Molecular functionActivator, Initiation factor, Repressor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-32002-MONOMER
ReactomeiR-SCE-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor eIF-2B subunit alpha
Alternative name(s):
GCD complex subunit GCN3
Guanine nucleotide exchange factor subunit GCN3
Transcriptional activator GCN3
eIF-2B GDP-GTP exchange factor subunit alpha
Gene namesi
Name:GCN3
Synonyms:AAS2, TIF221
Ordered Locus Names:YKR026C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKR026C
SGDiS000001734 GCN3

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001560602 – 305Translation initiation factor eIF-2B subunit alphaAdd BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei291PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP14741
PaxDbiP14741
PRIDEiP14741

PTM databases

iPTMnetiP14741

Interactioni

Subunit structurei

Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34158, 148 interactors
DIPiDIP-1328N
IntActiP14741, 82 interactors
MINTiP14741
STRINGi4932.YKR026C

Structurei

3D structure databases

ProteinModelPortaliP14741
SMRiP14741
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074853
HOGENOMiHOG000175731
InParanoidiP14741
KOiK03239
OMAiQYLVGMF
OrthoDBiEOG092C2Z6R

Family and domain databases

InterProiView protein in InterPro
IPR000649 IF-2B-related
IPR037171 NagB/RpiA_transferase-like
PfamiView protein in Pfam
PF01008 IF-2B, 1 hit
SUPFAMiSSF100950 SSF100950, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14741-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFNITETY LRFLEEDTEM TMPIAAIEAL VTLLRIKTPE TAAEMINTIK
60 70 80 90 100
SSTEELIKSI PNSVSLRAGC DIFMRFVLRN LHLYGDWENC KQHLIENGQL
110 120 130 140 150
FVSRAKKSRN KIAEIGVDFI ADDDIILVHG YSRAVFSLLN HAANKFIRFR
160 170 180 190 200
CVVTESRPSK QGNQLYTLLE QKGIPVTLIV DSAVGAVIDK VDKVFVGAEG
210 220 230 240 250
VAESGGIINL VGTYSVGVLA HNARKPFYVV TESHKFVRMF PLSSDDLPMA
260 270 280 290 300
GPPLDFTRRT DDLEDALRGP TIDYTAQEYI TALITDLGVL TPSAVSEELI

KMWYD
Length:305
Mass (Da):34,025
Last modified:April 1, 1990 - v1
Checksum:i8E9A928D6D56E12B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23356 Genomic DNA Translation: AAA34637.1
Z28251 Genomic DNA Translation: CAA82098.1
BK006944 Genomic DNA Translation: DAA09181.1
PIRiA31562
RefSeqiNP_012951.1, NM_001179816.1

Genome annotation databases

EnsemblFungiiYKR026C; YKR026C; YKR026C
GeneIDi853896
KEGGisce:YKR026C

Similar proteinsi

Entry informationi

Entry nameiEI2BA_YEAST
AccessioniPrimary (citable) accession number: P14741
Secondary accession number(s): D6VX91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 23, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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