P14741 (EI2BA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor eIF-2B subunit alpha

Alternative name(s):
GCD complex subunit GCN3
Guanine nucleotide exchange factor subunit GCN3
Transcriptional activator GCN3
eIF-2B GDP-GTP exchange factor subunit alpha

Gene names

Name:	GCN3
Synonyms:	AAS2, TIF221
Ordered Locus Names:	YKR026C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a non-essential regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. Modulation of GCN3 regulatory function in response to amino acid availability occurs post-translationally. Ref.4 Ref.5
Subunit structure	Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro. Ref.4 Ref.5
Miscellaneous	Present with 8970 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the eIF-2B alpha/beta/delta subunits family.

Ontologies

Keywords
Biological process	Protein biosynthesis Translation regulation
Molecular function	Activator Initiation factor Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of translational initiation Inferred from direct assay Ref.4. Source: SGD
Cellular_component	eukaryotic translation initiation factor 2B complex Inferred from direct assay PubMed 8336705Ref.4. Source: SGD guanyl-nucleotide exchange factor complex Inferred from direct assay Ref.4. Source: SGD
Molecular_function	enzyme regulator activity Inferred from mutant phenotype PubMed 8099443PubMed 8887689PubMed 9032257. Source: SGD translation initiation factor activity Inferred from direct assay PubMed 8336705. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		4	EBI-6253,EBI-6253
BIT2	P38346	3	EBI-6253,EBI-21144
GCD2	P12754	5	EBI-6253,EBI-6265
GCD7	P32502	9	EBI-6253,EBI-6260

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Translation initiation factor eIF-2B subunit alpha		PRO_0000156060
Amino acid modifications
	Modified residue	291	1	Phosphothreonine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P14741 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 8E9A928D6D56E12B	FASTA	305	34,025
	10 20 30 40 50 60 MSEFNITETY LRFLEEDTEM TMPIAAIEAL VTLLRIKTPE TAAEMINTIK SSTEELIKSI 70 80 90 100 110 120 PNSVSLRAGC DIFMRFVLRN LHLYGDWENC KQHLIENGQL FVSRAKKSRN KIAEIGVDFI 130 140 150 160 170 180 ADDDIILVHG YSRAVFSLLN HAANKFIRFR CVVTESRPSK QGNQLYTLLE QKGIPVTLIV 190 200 210 220 230 240 DSAVGAVIDK VDKVFVGAEG VAESGGIINL VGTYSVGVLA HNARKPFYVV TESHKFVRMF 250 260 270 280 290 300 PLSSDDLPMA GPPLDFTRRT DDLEDALRGP TIDYTAQEYI TALITDLGVL TPSAVSEELI KMWYD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of GCN3, a translational activator of GCN4: evidence for posttranslational control of GCN3 regulatory function." Hannig E.M., Hinnebusch A.G. Mol. Cell. Biol. 8:4808-4820(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast." Cigan A.M., Bushman J.L., Boal T.R., Hinnebusch A.G. Proc. Natl. Acad. Sci. U.S.A. 90:5350-5354(1993) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE EIF2-B COMPLEX, FUNCTION OF THE EIF2-B COMPLEX.
[5]	"eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange." Pavitt G.D., Ramaiah K.V., Kimball S.R., Hinnebusch A.G. Genes Dev. 12:514-526(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN A EIF2-B SUBCOMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-291, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23356 Genomic DNA. Translation: AAA34637.1. Z28251 Genomic DNA. Translation: CAA82098.1. BK006944 Genomic DNA. Translation: DAA09181.1.
PIR	A31562.
RefSeq	NP_012951.1. NM_001179816.1.
3D structure databases
ProteinModelPortal	P14741.
SMR	P14741. Positions 1-303.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1328N.
IntAct	P14741. 47 interactions.
MINT	MINT-385513.
STRING	4932.YKR026C.
Proteomic databases
PaxDb	P14741.
PeptideAtlas	P14741.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKR026C; YKR026C; YKR026C.
GeneID	853896.
KEGG	sce:YKR026C.
Organism-specific databases
CYGD	YKR026c.
SGD	S000001734. GCN3.
Phylogenomic databases
eggNOG	COG1184.
GeneTree	ENSGT00550000074853.
HOGENOM	HOG000175731.
KO	K03239.
OMA	HPLVDYT.
OrthoDB	EOG46MFTJ.
Gene expression databases
Genevestigator	P14741.
GermOnline	YKR026C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000649. IF-2B-related. [Graphical view]
PANTHER	PTHR10233. PTHR10233. 1 hit.
Pfam	PF01008. IF-2B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	975213.

Entry information

Entry name

EI2BA_YEAST

Accession

Primary (citable) accession number: P14741
Secondary accession number(s): D6VX91

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program