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P14741

- EI2BA_YEAST

UniProt

P14741 - EI2BA_YEAST

Protein

Translation initiation factor eIF-2B subunit alpha

Gene

GCN3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    Acts as a non-essential regulatory component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. Modulation of GCN3 regulatory function in response to amino acid availability occurs post-translationally.2 Publications

    GO - Molecular functioni

    1. enzyme regulator activity Source: SGD
    2. identical protein binding Source: IntAct
    3. protein binding Source: IntAct
    4. translation initiation factor activity Source: SGD

    GO - Biological processi

    1. positive regulation of GTPase activity Source: GOC
    2. regulation of catalytic activity Source: GOC
    3. regulation of translational initiation Source: SGD

    Keywords - Molecular functioni

    Activator, Initiation factor, Repressor

    Keywords - Biological processi

    Protein biosynthesis, Translation regulation

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32002-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Translation initiation factor eIF-2B subunit alpha
    Alternative name(s):
    GCD complex subunit GCN3
    Guanine nucleotide exchange factor subunit GCN3
    Transcriptional activator GCN3
    eIF-2B GDP-GTP exchange factor subunit alpha
    Gene namesi
    Name:GCN3
    Synonyms:AAS2, TIF221
    Ordered Locus Names:YKR026C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKR026c.
    SGDiS000001734. GCN3.

    Subcellular locationi

    GO - Cellular componenti

    1. eukaryotic translation initiation factor 2B complex Source: SGD
    2. guanyl-nucleotide exchange factor complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 305304Translation initiation factor eIF-2B subunit alphaPRO_0000156060Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei291 – 2911Phosphothreonine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP14741.
    PaxDbiP14741.
    PeptideAtlasiP14741.

    Expressioni

    Gene expression databases

    GenevestigatoriP14741.

    Interactioni

    Subunit structurei

    Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-6253,EBI-6253
    BIT2P383463EBI-6253,EBI-21144
    GCD2P127543EBI-6253,EBI-6265
    GCD7P325027EBI-6253,EBI-6260

    Protein-protein interaction databases

    BioGridi34158. 93 interactions.
    DIPiDIP-1328N.
    IntActiP14741. 27 interactions.
    MINTiMINT-385513.
    STRINGi4932.YKR026C.

    Structurei

    3D structure databases

    ProteinModelPortaliP14741.
    SMRiP14741. Positions 1-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1184.
    GeneTreeiENSGT00550000074853.
    HOGENOMiHOG000175731.
    KOiK03239.
    OMAiTESHKFV.
    OrthoDBiEOG73BVPK.

    Family and domain databases

    InterProiIPR000649. IF-2B-related.
    [Graphical view]
    PfamiPF01008. IF-2B. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P14741-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEFNITETY LRFLEEDTEM TMPIAAIEAL VTLLRIKTPE TAAEMINTIK    50
    SSTEELIKSI PNSVSLRAGC DIFMRFVLRN LHLYGDWENC KQHLIENGQL 100
    FVSRAKKSRN KIAEIGVDFI ADDDIILVHG YSRAVFSLLN HAANKFIRFR 150
    CVVTESRPSK QGNQLYTLLE QKGIPVTLIV DSAVGAVIDK VDKVFVGAEG 200
    VAESGGIINL VGTYSVGVLA HNARKPFYVV TESHKFVRMF PLSSDDLPMA 250
    GPPLDFTRRT DDLEDALRGP TIDYTAQEYI TALITDLGVL TPSAVSEELI 300
    KMWYD 305
    Length:305
    Mass (Da):34,025
    Last modified:April 1, 1990 - v1
    Checksum:i8E9A928D6D56E12B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23356 Genomic DNA. Translation: AAA34637.1.
    Z28251 Genomic DNA. Translation: CAA82098.1.
    BK006944 Genomic DNA. Translation: DAA09181.1.
    PIRiA31562.
    RefSeqiNP_012951.1. NM_001179816.1.

    Genome annotation databases

    EnsemblFungiiYKR026C; YKR026C; YKR026C.
    GeneIDi853896.
    KEGGisce:YKR026C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M23356 Genomic DNA. Translation: AAA34637.1 .
    Z28251 Genomic DNA. Translation: CAA82098.1 .
    BK006944 Genomic DNA. Translation: DAA09181.1 .
    PIRi A31562.
    RefSeqi NP_012951.1. NM_001179816.1.

    3D structure databases

    ProteinModelPortali P14741.
    SMRi P14741. Positions 1-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34158. 93 interactions.
    DIPi DIP-1328N.
    IntActi P14741. 27 interactions.
    MINTi MINT-385513.
    STRINGi 4932.YKR026C.

    Proteomic databases

    MaxQBi P14741.
    PaxDbi P14741.
    PeptideAtlasi P14741.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKR026C ; YKR026C ; YKR026C .
    GeneIDi 853896.
    KEGGi sce:YKR026C.

    Organism-specific databases

    CYGDi YKR026c.
    SGDi S000001734. GCN3.

    Phylogenomic databases

    eggNOGi COG1184.
    GeneTreei ENSGT00550000074853.
    HOGENOMi HOG000175731.
    KOi K03239.
    OMAi TESHKFV.
    OrthoDBi EOG73BVPK.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32002-MONOMER.

    Miscellaneous databases

    NextBioi 975213.
    PROi P14741.

    Gene expression databases

    Genevestigatori P14741.

    Family and domain databases

    InterProi IPR000649. IF-2B-related.
    [Graphical view ]
    Pfami PF01008. IF-2B. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of GCN3, a translational activator of GCN4: evidence for posttranslational control of GCN3 regulatory function."
      Hannig E.M., Hinnebusch A.G.
      Mol. Cell. Biol. 8:4808-4820(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast."
      Cigan A.M., Bushman J.L., Boal T.R., Hinnebusch A.G.
      Proc. Natl. Acad. Sci. U.S.A. 90:5350-5354(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE EIF2-B COMPLEX, FUNCTION OF THE EIF2-B COMPLEX.
    5. "eIF2 independently binds two distinct eIF2B subcomplexes that catalyze and regulate guanine-nucleotide exchange."
      Pavitt G.D., Ramaiah K.V., Kimball S.R., Hinnebusch A.G.
      Genes Dev. 12:514-526(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN A EIF2-B SUBCOMPLEX.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-291, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiEI2BA_YEAST
    AccessioniPrimary (citable) accession number: P14741
    Secondary accession number(s): D6VX91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8970 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3