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P14736

- RAD4_YEAST

UniProt

P14736 - RAD4_YEAST

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Protein

DNA repair protein RAD4

Gene
RAD4, YER162C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi250 – 26920 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. nucleotide-excision repair Source: SGD
  2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30323-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD4
Gene namesi
Name:RAD4
Ordered Locus Names:YER162C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome V

Organism-specific databases

SGDiS000000964. RAD4.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
  2. nucleotide-excision repair factor 2 complex Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754DNA repair protein RAD4PRO_0000218298Add
BLAST

Proteomic databases

MaxQBiP14736.
PaxDbiP14736.
PeptideAtlasiP14736.

Expressioni

Gene expression databases

GenevestigatoriP14736.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD23P326283EBI-14766,EBI-14668

Protein-protein interaction databases

BioGridi36915. 134 interactions.
DIPiDIP-1547N.
IntActiP14736. 5 interactions.
MINTiMINT-396392.
STRINGi4932.YER162C.

Structurei

Secondary structure

1
754
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni91 – 944
Helixi124 – 1296
Helixi134 – 16229
Helixi165 – 1717
Helixi172 – 1743
Helixi177 – 1826
Helixi193 – 21321
Beta strandi221 – 2233
Helixi230 – 2356
Turni236 – 2394
Helixi248 – 25710
Helixi262 – 27514
Beta strandi280 – 2867
Helixi306 – 3094
Beta strandi314 – 3218
Turni322 – 3254
Beta strandi326 – 33510
Beta strandi337 – 3393
Turni352 – 3554
Beta strandi362 – 3665
Beta strandi372 – 3743
Helixi376 – 3794
Helixi383 – 3875
Helixi388 – 3903
Helixi392 – 3943
Helixi396 – 40914
Helixi416 – 43116
Beta strandi436 – 4383
Helixi439 – 4413
Beta strandi445 – 4495
Helixi450 – 4523
Beta strandi457 – 4593
Beta strandi466 – 4705
Beta strandi478 – 4836
Helixi484 – 4863
Beta strandi487 – 4893
Helixi493 – 4975
Turni498 – 5003
Beta strandi501 – 5033
Beta strandi510 – 5134
Beta strandi529 – 5324
Helixi534 – 5363
Beta strandi537 – 5393
Helixi564 – 5663
Beta strandi571 – 5755
Helixi579 – 5857
Beta strandi591 – 5977
Beta strandi601 – 6033
Beta strandi607 – 6159
Helixi616 – 6183
Helixi619 – 6279
Helixi629 – 6313

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2M14NMR-B76-115[»]
2QSFX-ray2.35A101-632[»]
2QSGX-ray3.10A101-632[»]
2QSHX-ray2.81A101-632[»]
ProteinModelPortaliP14736.
SMRiP14736. Positions 123-632.

Miscellaneous databases

EvolutionaryTraceiP14736.

Family & Domainsi

Sequence similaritiesi

Belongs to the XPC family.

Phylogenomic databases

eggNOGiCOG5535.
GeneTreeiENSGT00390000005194.
HOGENOMiHOG000074544.
KOiK10838.
OMAiCEVWDKF.
OrthoDBiEOG7H79C5.

Family and domain databases

InterProiIPR004583. DNA_repair_Rad4.
IPR018325. Rad4/PNGase_transGLS-fold.
IPR018326. Rad4_beta-hairpin_dom1.
IPR018327. Rad4_beta-hairpin_dom2.
IPR018328. Rad4_beta-hairpin_dom3.
[Graphical view]
PANTHERiPTHR12135. PTHR12135. 1 hit.
PfamiPF10403. BHD_1. 1 hit.
PF10404. BHD_2. 1 hit.
PF10405. BHD_3. 1 hit.
PF03835. Rad4. 1 hit.
[Graphical view]
SMARTiSM01030. BHD_1. 1 hit.
SM01031. BHD_2. 1 hit.
SM01032. BHD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14736-1 [UniParc]FASTAAdd to Basket

« Hide

MNEDLPKEYF ELIRKALNEK EAEKAPLSRR RRVRRKNQPL PDAKKKFKTG    50
LNELPRESVV TVNLDSSDDG VVTVPTDDSV EEIQSSEEDY DSEEFEDVTD 100
GNEVAGVEDI SVEIKPSSKR NSDARRTSRN VCSNEERKRR KYFHMLYLVC 150
LMVHGFIRNE WINSKRLSRK LSNLVPEKVF ELLHPQKDEE LPLRSTRKLL 200
DGLKKCMELW QKHWKITKKY DNVGLYMRTW KEIEMSANNK RKFKTLKRSD 250
FLRAVSKGHG DPDISVQGFV AMLRACNVNA RLIMSCQPPD FTNMKIDTSL 300
NGNNAYKDMV KYPIFWCEVW DKFSKKWITV DPVNLKTIEQ VRLHSKLAPK 350
GVACCERNML RYVIAYDRKY GCRDVTRRYA QWMNSKVRKR RITKDDFGEK 400
WFRKVITALH HRKRTKIDDY EDQYFFQRDE SEGIPDSVQD LKNHPYYVLE 450
QDIKQTQIVK PGCKECGYLK VHGKVGKVLK VYAKRDIADL KSARQWYMNG 500
RILKTGSRCK KVIKRTVGRP KGEAEEEDER LYSFEDTELY IPPLASASGE 550
ITKNTFGNIE VFAPTMIPGN CCLVENPVAI KAARFLGVEF APAVTSFKFE 600
RGSTVKPVLS GIVVAKWLRE AIETAIDGIE FIQEDDNRKE HLLGALESWN 650
TLLLKLRIRS KLNSTYGKIA EEEPNVTKEQ NIADNHDNTE TFMGGGFLPG 700
IANHEARPYS EPSEPEDSLD YVSVDKAEES ATDDDVGEDY SDFMKELEMS 750
EESD 754
Length:754
Mass (Da):87,174
Last modified:September 21, 2011 - v3
Checksum:i788D3B6F580D2BF8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti223 – 2231V → E in AAB64689. 1 Publication
Sequence conflicti225 – 2251L → I in AAA34944. 1 Publication
Sequence conflicti225 – 2251L → I in AAA34945. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26050 Genomic DNA. Translation: AAA34944.1.
M24928 Genomic DNA. Translation: AAA34945.1.
U18917 Genomic DNA. Translation: AAB64689.1.
BK006939 Genomic DNA. Translation: DAA07824.2.
PIRiS30814. DDBYD4.
RefSeqiNP_011089.4. NM_001179052.4.

Genome annotation databases

EnsemblFungiiYER162C; YER162C; YER162C.
GeneIDi856909.
KEGGisce:YER162C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26050 Genomic DNA. Translation: AAA34944.1 .
M24928 Genomic DNA. Translation: AAA34945.1 .
U18917 Genomic DNA. Translation: AAB64689.1 .
BK006939 Genomic DNA. Translation: DAA07824.2 .
PIRi S30814. DDBYD4.
RefSeqi NP_011089.4. NM_001179052.4.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2M14 NMR - B 76-115 [» ]
2QSF X-ray 2.35 A 101-632 [» ]
2QSG X-ray 3.10 A 101-632 [» ]
2QSH X-ray 2.81 A 101-632 [» ]
ProteinModelPortali P14736.
SMRi P14736. Positions 123-632.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36915. 134 interactions.
DIPi DIP-1547N.
IntActi P14736. 5 interactions.
MINTi MINT-396392.
STRINGi 4932.YER162C.

Proteomic databases

MaxQBi P14736.
PaxDbi P14736.
PeptideAtlasi P14736.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YER162C ; YER162C ; YER162C .
GeneIDi 856909.
KEGGi sce:YER162C.

Organism-specific databases

SGDi S000000964. RAD4.

Phylogenomic databases

eggNOGi COG5535.
GeneTreei ENSGT00390000005194.
HOGENOMi HOG000074544.
KOi K10838.
OMAi CEVWDKF.
OrthoDBi EOG7H79C5.

Enzyme and pathway databases

BioCyci YEAST:G3O-30323-MONOMER.

Miscellaneous databases

EvolutionaryTracei P14736.
NextBioi 983347.
PROi P14736.

Gene expression databases

Genevestigatori P14736.

Family and domain databases

InterProi IPR004583. DNA_repair_Rad4.
IPR018325. Rad4/PNGase_transGLS-fold.
IPR018326. Rad4_beta-hairpin_dom1.
IPR018327. Rad4_beta-hairpin_dom2.
IPR018328. Rad4_beta-hairpin_dom3.
[Graphical view ]
PANTHERi PTHR12135. PTHR12135. 1 hit.
Pfami PF10403. BHD_1. 1 hit.
PF10404. BHD_2. 1 hit.
PF10405. BHD_3. 1 hit.
PF03835. Rad4. 1 hit.
[Graphical view ]
SMARTi SM01030. BHD_1. 1 hit.
SM01031. BHD_2. 1 hit.
SM01032. BHD_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD4 gene required for excision repair of UV-damaged DNA."
    Gietz R.D., Prakash S.
    Gene 74:535-541(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Nucleotide sequence of the wild-type RAD4 gene of Saccharomyces cerevisiae and characterization of mutant rad4 alleles."
    Couto L.B., Friedberg E.C.
    J. Bacteriol. 171:1862-1869(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 223.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD4_YEAST
AccessioniPrimary (citable) accession number: P14736
Secondary accession number(s): D3DM70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: September 21, 2011
Last modified: July 9, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 876 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

External Data

Dasty 3

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