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P14736 (RAD4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA repair protein RAD4
Gene names
Name:RAD4
Ordered Locus Names:YER162C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length754 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in nucleotide excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents.

Subcellular location

Cytoplasm. Nucleus Ref.5.

Miscellaneous

Present with 876 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the XPC family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RAD23P326283EBI-14766,EBI-14668

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 754754DNA repair protein RAD4
PRO_0000218298

Regions

DNA binding250 – 26920 Potential

Experimental info

Sequence conflict2231V → E in AAB64689. Ref.3
Sequence conflict2251L → I in AAA34944. Ref.1
Sequence conflict2251L → I in AAA34945. Ref.2

Secondary structure

........................................................................................... 754
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P14736 [UniParc].

Last modified September 21, 2011. Version 3.
Checksum: 788D3B6F580D2BF8

FASTA75487,174
        10         20         30         40         50         60 
MNEDLPKEYF ELIRKALNEK EAEKAPLSRR RRVRRKNQPL PDAKKKFKTG LNELPRESVV 

        70         80         90        100        110        120 
TVNLDSSDDG VVTVPTDDSV EEIQSSEEDY DSEEFEDVTD GNEVAGVEDI SVEIKPSSKR 

       130        140        150        160        170        180 
NSDARRTSRN VCSNEERKRR KYFHMLYLVC LMVHGFIRNE WINSKRLSRK LSNLVPEKVF 

       190        200        210        220        230        240 
ELLHPQKDEE LPLRSTRKLL DGLKKCMELW QKHWKITKKY DNVGLYMRTW KEIEMSANNK 

       250        260        270        280        290        300 
RKFKTLKRSD FLRAVSKGHG DPDISVQGFV AMLRACNVNA RLIMSCQPPD FTNMKIDTSL 

       310        320        330        340        350        360 
NGNNAYKDMV KYPIFWCEVW DKFSKKWITV DPVNLKTIEQ VRLHSKLAPK GVACCERNML 

       370        380        390        400        410        420 
RYVIAYDRKY GCRDVTRRYA QWMNSKVRKR RITKDDFGEK WFRKVITALH HRKRTKIDDY 

       430        440        450        460        470        480 
EDQYFFQRDE SEGIPDSVQD LKNHPYYVLE QDIKQTQIVK PGCKECGYLK VHGKVGKVLK 

       490        500        510        520        530        540 
VYAKRDIADL KSARQWYMNG RILKTGSRCK KVIKRTVGRP KGEAEEEDER LYSFEDTELY 

       550        560        570        580        590        600 
IPPLASASGE ITKNTFGNIE VFAPTMIPGN CCLVENPVAI KAARFLGVEF APAVTSFKFE 

       610        620        630        640        650        660 
RGSTVKPVLS GIVVAKWLRE AIETAIDGIE FIQEDDNRKE HLLGALESWN TLLLKLRIRS 

       670        680        690        700        710        720 
KLNSTYGKIA EEEPNVTKEQ NIADNHDNTE TFMGGGFLPG IANHEARPYS EPSEPEDSLD 

       730        740        750 
YVSVDKAEES ATDDDVGEDY SDFMKELEMS EESD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and nucleotide sequence analysis of the Saccharomyces cerevisiae RAD4 gene required for excision repair of UV-damaged DNA."
Gietz R.D., Prakash S.
Gene 74:535-541(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Nucleotide sequence of the wild-type RAD4 gene of Saccharomyces cerevisiae and characterization of mutant rad4 alleles."
Couto L.B., Friedberg E.C.
J. Bacteriol. 171:1862-1869(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 223.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26050 Genomic DNA. Translation: AAA34944.1.
M24928 Genomic DNA. Translation: AAA34945.1.
U18917 Genomic DNA. Translation: AAB64689.1.
BK006939 Genomic DNA. Translation: DAA07824.2.
PIRDDBYD4. S30814.
RefSeqNP_011089.4. NM_001179052.4.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2M14NMR-B76-115[»]
2QSFX-ray2.35A101-632[»]
2QSGX-ray3.10A101-632[»]
2QSHX-ray2.81A101-632[»]
ProteinModelPortalP14736.
SMRP14736. Positions 123-632.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36915. 132 interactions.
DIPDIP-1547N.
IntActP14736. 5 interactions.
MINTMINT-396392.
STRING4932.YER162C.

Proteomic databases

PaxDbP14736.
PeptideAtlasP14736.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER162C; YER162C; YER162C.
GeneID856909.
KEGGsce:YER162C.

Organism-specific databases

SGDS000000964. RAD4.

Phylogenomic databases

eggNOGCOG5535.
GeneTreeENSGT00390000005194.
HOGENOMHOG000074544.
KOK10838.
OMACEVWDKF.
OrthoDBEOG7H79C5.

Enzyme and pathway databases

BioCycYEAST:G3O-30323-MONOMER.

Gene expression databases

GenevestigatorP14736.

Family and domain databases

InterProIPR004583. DNA_repair_Rad4.
IPR018325. Rad4/PNGase_transGLS-fold.
IPR018326. Rad4_beta-hairpin_dom1.
IPR018327. Rad4_beta-hairpin_dom2.
IPR018328. Rad4_beta-hairpin_dom3.
[Graphical view]
PANTHERPTHR12135. PTHR12135. 1 hit.
PfamPF10403. BHD_1. 1 hit.
PF10404. BHD_2. 1 hit.
PF10405. BHD_3. 1 hit.
PF03835. Rad4. 1 hit.
[Graphical view]
SMARTSM01030. BHD_1. 1 hit.
SM01031. BHD_2. 1 hit.
SM01032. BHD_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP14736.
NextBio983347.
PROP14736.

Entry information

Entry nameRAD4_YEAST
AccessionPrimary (citable) accession number: P14736
Secondary accession number(s): D3DM70
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: September 21, 2011
Last modified: April 16, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references