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Protein

Lamin-B1

Gene

Lmnb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Miscellaneous

The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.

GO - Molecular functioni

  • JUN kinase binding Source: BHF-UCL
  • phospholipase binding Source: BHF-UCL
  • structural molecule activity Source: InterPro

GO - Biological processi

  • positive regulation of G2/M transition of mitotic cell cycle Source: BHF-UCL
  • positive regulation of JNK cascade Source: BHF-UCL

Enzyme and pathway databases

ReactomeiR-MMU-2559584 Formation of Senescence-Associated Heterochromatin Foci (SAHF)
R-MMU-352238 Breakdown of the nuclear lamina
R-MMU-4419969 Depolymerisation of the Nuclear Lamina

Names & Taxonomyi

Protein namesi
Recommended name:
Lamin-B1
Gene namesi
Name:Lmnb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:96795 Lmnb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000638172 – 585Lamin-B1Add BLAST584
PropeptideiPRO_0000403467586 – 588Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei3PhosphothreonineBy similarity1
Modified residuei5PhosphothreonineBy similarity1
Modified residuei15Omega-N-methylarginineBy similarity1
Modified residuei17PhosphoserineCombined sources1
Modified residuei21PhosphothreonineCombined sources1
Modified residuei24PhosphoserineCombined sources1
Modified residuei26PhosphothreonineBy similarity1
Modified residuei29PhosphoserineBy similarity1
Cross-linki103Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei112N6-acetyllysineCombined sources1
Cross-linki124Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei127PhosphoserineBy similarity1
Cross-linki146Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei158N6-acetyllysine; alternateBy similarity1
Cross-linki158Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei159PhosphoserineBy similarity1
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei201PhosphoserineBy similarity1
Modified residuei233PhosphoserineBy similarity1
Cross-linki242Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki262Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei272N6-acetyllysine; alternateCombined sources1
Cross-linki272Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei279PhosphoserineBy similarity1
Modified residuei303PhosphoserineBy similarity1
Cross-linki313Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Disulfide bondi318InterchainBy similarity
Modified residuei331N6-acetyllysine; alternateCombined sources1
Cross-linki331Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei376PhosphoserineBy similarity1
Modified residuei414Omega-N-methylarginineBy similarity1
Modified residuei484N6-acetyllysineBy similarity1
Cross-linki533Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei535PhosphoserineBy similarity1
Cross-linki548Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei585Cysteine methyl esterBy similarity1
Lipidationi585S-farnesyl cysteineBy similarity1

Post-translational modificationi

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Keywords - PTMi

Acetylation, Disulfide bond, Isopeptide bond, Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugation

Proteomic databases

EPDiP14733
MaxQBiP14733
PaxDbiP14733
PeptideAtlasiP14733
PRIDEiP14733

2D gel databases

REPRODUCTION-2DPAGEiIPI00230394
SWISS-2DPAGEiP14733

PTM databases

iPTMnetiP14733
PhosphoSitePlusiP14733
SwissPalmiP14733

Expressioni

Gene expression databases

BgeeiENSMUSG00000024590
CleanExiMM_LMNB1
GenevisibleiP14733 MM

Interactioni

Subunit structurei

Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12 (By similarity).By similarity

GO - Molecular functioni

  • JUN kinase binding Source: BHF-UCL
  • phospholipase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi201177, 12 interactors
IntActiP14733, 11 interactors
MINTiP14733
STRINGi10090.ENSMUSP00000025486

Structurei

3D structure databases

ProteinModelPortaliP14733
SMRiP14733
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 389IF rodPROSITE-ProRule annotationAdd BLAST357
Domaini431 – 547LTDPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 35HeadAdd BLAST34
Regioni36 – 70Coil 1AAdd BLAST35
Regioni71 – 82Linker 1Add BLAST12
Regioni83 – 216Coil 1BAdd BLAST134
Regioni217 – 244Linker 2Add BLAST28
Regioni245 – 387Coil 2Add BLAST143
Regioni388 – 588TailAdd BLAST201

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi416 – 421Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi553 – 561Glu-rich (highly acidic; could be involved in chromatin binding)9

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0977 Eukaryota
ENOG410Y2H6 LUCA
GeneTreeiENSGT00910000143989
HOGENOMiHOG000007711
HOVERGENiHBG013015
InParanoidiP14733
KOiK07611
OMAiFHQQGAP
OrthoDBiEOG091G063B
PhylomeDBiP14733
TreeFamiTF101181

Family and domain databases

Gene3Di2.60.40.1260, 1 hit
InterProiView protein in InterPro
IPR001664 IF
IPR018039 IF_conserved
IPR039008 IF_rod_dom
IPR001322 Lamin_tail_dom
IPR036415 Lamin_tail_dom_sf
PANTHERiPTHR23239 PTHR23239, 1 hit
PfamiView protein in Pfam
PF00038 Filament, 1 hit
PF00932 LTD, 1 hit
SMARTiView protein in SMART
SM01391 Filament, 1 hit
SUPFAMiSSF74853 SSF74853, 1 hit
PROSITEiView protein in PROSITE
PS00226 IF_ROD_1, 1 hit
PS51842 IF_ROD_2, 1 hit
PS51841 LTD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14733-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATATPVQQQ RAGSRASAPA TPLSPTRLSR LQEKEELREL NDRLAVYIDK
60 70 80 90 100
VRSLETENSA LQLQVTEREE VRGRELTGLK ALYETELADA RRALDDTARE
110 120 130 140 150
RAKLQIELGK FKAEHDQLLL NYAKKESDLS GAQIKLREYE AALNSKDAAL
160 170 180 190 200
ATALGDKKSL EGDLEDLKDQ IAQLEASLSA AKKQLADETL LKVDLENRCQ
210 220 230 240 250
SLTEDLEFRK NMYEEEINET RRKHETRLVE VDSGRQIEYE YKLAQALHEM
260 270 280 290 300
REQHDAQVRL YKEELEQTYH AKLENARLSS EMNTSTVNSA REELMESRMR
310 320 330 340 350
IESLSSQLSN LQKESRACLE RIQELEDMLA KERDNSRRML SDREREMAEI
360 370 380 390 400
RDQMQQQLSD YEQLLDVKLA LDMEISAYRK LLEGEEERLK LSPSPSSRVT
410 420 430 440 450
VSRASSSRSV RTTRGKRKRV DVEESEASSS VSISHSASAT GNVCIEEIDV
460 470 480 490 500
DGKFIRLKNT SEQDQPMGGW EMIRKIGDTS VSYKYTSRYV LKAGQTVTVW
510 520 530 540 550
AANAGVTASP PTDLIWKNQN SWGTGEDVKV ILKNSQGEEV AQRSTVFKTT
560 570 580
IPEEEEEEEE EPIGVAVEEE RFHQQGAPRA SNKSCAIM
Length:588
Mass (Da):66,786
Last modified:January 23, 2007 - v3
Checksum:i3602BCE63588A32D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti581S → W in CAA34677 (PubMed:3243285).Curated1
Sequence conflicti581S → W in AAC96023 (PubMed:3243285).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16705 mRNA Translation: CAA34677.1
M35153 mRNA Translation: AAC96023.1 Sequence problems.
D50080 Genomic DNA Translation: BAA08784.1
BC052729 mRNA Translation: AAH52729.1
BC058392 mRNA Translation: AAH58392.1
CCDSiCCDS29261.1
PIRiS07720
RefSeqiNP_034851.2, NM_010721.2
UniGeneiMm.4105

Genome annotation databases

EnsembliENSMUST00000025486; ENSMUSP00000025486; ENSMUSG00000024590
GeneIDi16906
KEGGimmu:16906
UCSCiuc012bdd.2 mouse

Similar proteinsi

Entry informationi

Entry nameiLMNB1_MOUSE
AccessioniPrimary (citable) accession number: P14733
Secondary accession number(s): Q61791
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 182 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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