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Protein

Lamin-B1

Gene

Lmnb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Miscellaneous

The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.

GO - Molecular functioni

  • JUN kinase binding Source: BHF-UCL
  • phospholipase binding Source: BHF-UCL
  • structural molecule activity Source: InterPro

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1221632. Meiotic synapsis.
R-MMU-1221633. Meiotic Synapsis.
R-MMU-2559584. Formation of Senescence-Associated Heterochromatin Foci (SAHF).
R-MMU-352238. Breakdown of the nuclear lamina.
R-MMU-8862803. Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models.

Names & Taxonomyi

Protein namesi
Recommended name:
Lamin-B1
Gene namesi
Name:Lmnb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:96795. Lmnb1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Intermediate filament, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000638172 – 585Lamin-B1Add BLAST584
PropeptideiPRO_0000403467586 – 588Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei3PhosphothreonineBy similarity1
Modified residuei5PhosphothreonineBy similarity1
Modified residuei15Omega-N-methylarginineBy similarity1
Modified residuei17PhosphoserineCombined sources1
Modified residuei21PhosphothreonineCombined sources1
Modified residuei24PhosphoserineCombined sources1
Modified residuei26PhosphothreonineBy similarity1
Modified residuei29PhosphoserineBy similarity1
Cross-linki103Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei112N6-acetyllysineCombined sources1
Cross-linki124Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei127PhosphoserineBy similarity1
Cross-linki146Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei158N6-acetyllysine; alternateBy similarity1
Cross-linki158Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei159PhosphoserineBy similarity1
Cross-linki182Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei201PhosphoserineBy similarity1
Modified residuei233PhosphoserineBy similarity1
Cross-linki242Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki262Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei272N6-acetyllysine; alternateCombined sources1
Cross-linki272Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei279PhosphoserineBy similarity1
Modified residuei303PhosphoserineBy similarity1
Cross-linki313Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Disulfide bondi318InterchainBy similarity
Modified residuei331N6-acetyllysine; alternateCombined sources1
Cross-linki331Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei376PhosphoserineBy similarity1
Modified residuei414Omega-N-methylarginineBy similarity1
Modified residuei484N6-acetyllysineBy similarity1
Cross-linki533Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei535PhosphoserineBy similarity1
Cross-linki548Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei585Cysteine methyl esterBy similarity1
Lipidationi585S-farnesyl cysteineBy similarity1

Post-translational modificationi

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Keywords - PTMi

Acetylation, Disulfide bond, Isopeptide bond, Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugation

Proteomic databases

EPDiP14733.
MaxQBiP14733.
PaxDbiP14733.
PeptideAtlasiP14733.
PRIDEiP14733.

2D gel databases

REPRODUCTION-2DPAGEiIPI00230394.
SWISS-2DPAGEiP14733.

PTM databases

iPTMnetiP14733.
PhosphoSitePlusiP14733.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024590.
CleanExiMM_LMNB1.
GenevisibleiP14733. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with lamin-associated polypeptides IA, IB and 2. Interacts with SPAG4 and SEPT12 (By similarity).By similarity

GO - Molecular functioni

  • JUN kinase binding Source: BHF-UCL
  • phospholipase binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi201177. 7 interactors.
IntActiP14733. 8 interactors.
MINTiMINT-1726964.
STRINGi10090.ENSMUSP00000025486.

Structurei

3D structure databases

ProteinModelPortaliP14733.
SMRiP14733.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 389IF rodPROSITE-ProRule annotationAdd BLAST357
Domaini431 – 547LTDPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 35HeadAdd BLAST34
Regioni36 – 70Coil 1AAdd BLAST35
Regioni71 – 82Linker 1Add BLAST12
Regioni83 – 216Coil 1BAdd BLAST134
Regioni217 – 244Linker 2Add BLAST28
Regioni245 – 387Coil 2Add BLAST143
Regioni388 – 588TailAdd BLAST201

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi416 – 421Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi553 – 561Glu-rich (highly acidic; could be involved in chromatin binding)9

Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0977. Eukaryota.
ENOG410Y2H6. LUCA.
GeneTreeiENSGT00830000128282.
HOGENOMiHOG000007711.
HOVERGENiHBG013015.
InParanoidiP14733.
KOiK07611.
OMAiFHQQGAP.
OrthoDBiEOG091G063B.
PhylomeDBiP14733.
TreeFamiTF101181.

Family and domain databases

Gene3Di2.60.40.1260. 1 hit.
InterProiView protein in InterPro
IPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR001322. Lamin_tail_dom.
IPR036415. Lamin_tail_dom_sf.
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiView protein in Pfam
PF00038. Filament. 1 hit.
PF00932. LTD. 1 hit.
SMARTiView protein in SMART
SM01391. Filament. 1 hit.
SUPFAMiSSF74853. SSF74853. 1 hit.
PROSITEiView protein in PROSITE
PS00226. IF_ROD_1. 1 hit.
PS51842. IF_ROD_2. 1 hit.
PS51841. LTD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14733-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATATPVQQQ RAGSRASAPA TPLSPTRLSR LQEKEELREL NDRLAVYIDK
60 70 80 90 100
VRSLETENSA LQLQVTEREE VRGRELTGLK ALYETELADA RRALDDTARE
110 120 130 140 150
RAKLQIELGK FKAEHDQLLL NYAKKESDLS GAQIKLREYE AALNSKDAAL
160 170 180 190 200
ATALGDKKSL EGDLEDLKDQ IAQLEASLSA AKKQLADETL LKVDLENRCQ
210 220 230 240 250
SLTEDLEFRK NMYEEEINET RRKHETRLVE VDSGRQIEYE YKLAQALHEM
260 270 280 290 300
REQHDAQVRL YKEELEQTYH AKLENARLSS EMNTSTVNSA REELMESRMR
310 320 330 340 350
IESLSSQLSN LQKESRACLE RIQELEDMLA KERDNSRRML SDREREMAEI
360 370 380 390 400
RDQMQQQLSD YEQLLDVKLA LDMEISAYRK LLEGEEERLK LSPSPSSRVT
410 420 430 440 450
VSRASSSRSV RTTRGKRKRV DVEESEASSS VSISHSASAT GNVCIEEIDV
460 470 480 490 500
DGKFIRLKNT SEQDQPMGGW EMIRKIGDTS VSYKYTSRYV LKAGQTVTVW
510 520 530 540 550
AANAGVTASP PTDLIWKNQN SWGTGEDVKV ILKNSQGEEV AQRSTVFKTT
560 570 580
IPEEEEEEEE EPIGVAVEEE RFHQQGAPRA SNKSCAIM
Length:588
Mass (Da):66,786
Last modified:January 23, 2007 - v3
Checksum:i3602BCE63588A32D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti581S → W in CAA34677 (PubMed:3243285).Curated1
Sequence conflicti581S → W in AAC96023 (PubMed:3243285).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16705 mRNA. Translation: CAA34677.1.
M35153 mRNA. Translation: AAC96023.1. Sequence problems.
D50080 Genomic DNA. Translation: BAA08784.1.
BC052729 mRNA. Translation: AAH52729.1.
BC058392 mRNA. Translation: AAH58392.1.
CCDSiCCDS29261.1.
PIRiS07720.
RefSeqiNP_034851.2. NM_010721.2.
UniGeneiMm.4105.

Genome annotation databases

EnsembliENSMUST00000025486; ENSMUSP00000025486; ENSMUSG00000024590.
GeneIDi16906.
KEGGimmu:16906.
UCSCiuc012bdd.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiLMNB1_MOUSE
AccessioniPrimary (citable) accession number: P14733
Secondary accession number(s): Q61791
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 176 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families