P14732 (LMNB2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lamin-B2

Gene names

Name:

LMNB2

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Subcellular location	Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
Post-translational modification	B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
Miscellaneous	The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.
Sequence similarities	Belongs to the intermediate filament family.

Ontologies

Keywords
Cellular component	Intermediate filament Membrane Nucleus
Domain	Coiled coil
PTM	Acetylation Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	intermediate filament Inferred from electronic annotation. Source: UniProtKB-KW nuclear inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 597	596	Lamin-B2		PRO_0000063822
	Propeptide	598 – 600	3	Removed in mature form By similarity		PRO_0000403472
Regions
	Region	2 – 27	26	Head
	Region	28 – 379	352	Rod
	Region	28 – 64	37	Coil 1A
	Region	75 – 212	138	Coil 1B
	Region	237 – 379	143	Coil 2
	Region	380 – 600	221	Tail
	Motif	414 – 419	6	Nuclear localization signal Potential
	Compositional bias	398 – 406	9	Poly-Ser
	Compositional bias	435 – 444	10	Poly-Ser
	Compositional bias	568 – 582	15	Asp/Glu-rich (highly acidic; could be involved in chromatin binding)
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	597	1	Cysteine methyl ester By similarity
	Lipidation	597	1	S-farnesyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14732 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 670E2985202211C0	FASTA	600	67,941
	10 20 30 40 50 60 MSGTPIRGTP GGTPLSPTRI SRLQEKEELR QLNDRLAVYI DRVRALELEN DRLLVKISEK 70 80 90 100 110 120 EEVTTREVSG IKNLYESELA DARRVLDETA KERARLQIEI GKLRAELEEF NKSYKKKDAD 130 140 150 160 170 180 LSVAQGRIKD LEVLFHRSEA ELNTVLNEKR SLEAEVADLR AQLAKAEDGH AVAKKQLEKE 190 200 210 220 230 240 TLMRVDLENR CQSLQEDLDF RKNVFEEEIR ETRKRHEHRL VEVDTSRQQE YENKMAQALE 250 260 270 280 290 300 DLRNQHDEQV KLYKMELEQT YQAKLENAIL ASDQNDKAAG AAREELKEAR MRIESLSHQL 310 320 330 340 350 360 SGLQKQASAT EDRIRELKET MAGERDKFRK MLDAKEREMT EMRDQMQLQL TEYQELLDVK 370 380 390 400 410 420 LALDMEISAY RKLLEGEEER LKLSPSPSSR VTVSRATSSS SSSSTSLVRS SRGKRRRIEA 430 440 450 460 470 480 EELSGSGTSG IGTGSISGSS SSSSFQMSQQ ASATGSISIE EIDLEGKYVQ LKNNSEKDQS 490 500 510 520 530 540 LGNWRLKRQI GDGEEIAYKF TPKYVLRAGQ TVTIWGADAG VSHSPPSVLV WKNQGSWGTG 550 560 570 580 590 600 GNIRTYLVNS DGEEVAVRTV TKSVVVRENE EEEDEADFGE EDLFNQQGDP RTTSRGCLVM

References

[1]	"A second higher vertebrate B-type lamin. cDNA sequence determination and in vitro processing of chicken lamin B2." Vorburger K., Lehner C.F., Kitten G.T., Eppenberger H.M., Nigg E.A. J. Mol. Biol. 208:405-415(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16880 mRNA. Translation: CAA34763.1.
IPI	IPI00601210.
PIR	S05519.
RefSeq	NP_990616.1. NM_205285.1.
UniGene	Gga.16090.
3D structure databases
ProteinModelPortal	P14732.
SMR	P14732. Positions 23-59, 308-380, 452-560.
ModBase	Search...
Protein-protein interaction databases
IntAct	P14732. 1 interaction.
STRING	9031.ENSGALP00000034565.
Proteomic databases
PaxDb	P14732.
PRIDE	P14732.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396222.
KEGG	gga:396222.
Organism-specific databases
CTD	84823.
Phylogenomic databases
eggNOG	NOG325506.
HOGENOM	HOG000007711.
HOVERGEN	HBG013015.
InParanoid	P14732.
KO	K07611.
OrthoDB	EOG4ZCT4D.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. IPR018039. Intermediate_filament_CS. IPR001322. Lamin_tail_dom. [Graphical view]
PANTHER	PTHR23239. PTHR23239. 1 hit.
Pfam	PF00038. Filament. 1 hit. PF00932. LTD. 1 hit. [Graphical view]
PROSITE	PS00226. IF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816274.

Entry information

Entry name

LMNB2_CHICK

Accession

Primary (citable) accession number: P14732

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program