P14724 (CDC26_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anaphase-promoting complex subunit CDC26

Alternative name(s):
Cell division control protein 26

Gene names

Name:	CDC26
Synonyms:	HIT3, SCD26
Ordered Locus Names:	YFR036W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	124 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin-protein ligase complex that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is thought to confer substrate specificity and, in the presence of ubiquitin-conjugating E2 enzymes, it catalyzes the formation of protein-ubiquitin conjugates that are subsequently degraded by the 26S proteasome. In early mitosis, the APC/C is activated by CDC20 and targets securin PDS1, the B-type cyclin CLB5, and other anaphase inhibitory proteins for proteolysis, thereby triggering the separation of sister chromatids at the metaphase-to-anaphase transition. In late mitosis and in G1, degradation of CLB5 allows activation of the APC/C by CDH1, which is needed to destroy CDC20 and the B-type cyclin CLB2 to allow exit from mitosis and creating the low CDK state necessary for cytokinesis and for reforming prereplicative complexes in G1 prior to another round of replication.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	The APC/C is composed of at least 13 subunits that stay tightly associated throughout the cell cycle: APC1, APC2, APC4, APC5, APC9, APC11, CDC16, CDC23, CDC26, CDC27, DOC1, MND2 and SWM1. CDC26 is required to tether the essential subunits APC9, CDC27 and CDC16 to the complex. Ref.7 Ref.8
Subcellular location	Nucleus Ref.9.
Sequence similarities	Belongs to the CDC26 family.
Caution	Was originally (Ref.1) thought to be a suppressor of CDC26.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from direct assay Ref.7. Source: SGD cell division Inferred from electronic annotation. Source: UniProtKB-KW chromatin assembly Inferred from genetic interaction. Source: SGD cyclin catabolic process Traceable author statement. Source: SGD mitotic metaphase/anaphase transition Traceable author statement. Source: SGD mitotic sister chromatid segregation Traceable author statement. Source: SGD mitotic spindle elongation Traceable author statement. Source: SGD regulation of mitotic metaphase/anaphase transition Inferred from electronic annotation. Source: InterPro
Cellular component	anaphase-promoting complex Inferred from direct assay. Source: SGD
Molecular function	ubiquitin-protein ligase activity Traceable author statement. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 124	124	Anaphase-promoting complex subunit CDC26		PRO_0000089446
Regions
	Compositional bias	53 – 77	25	Asp-rich
Experimental info
	Sequence conflict	50	1	S → F in CAA34978. Ref.1
	Sequence conflict	121 – 124	4	IREE → TARNNSISAYAHAHAHAHAS TFTFTYKISSL in CAA34978. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P14724 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: F41EECD2B56C4FB5	FASTA	124	14,081
	10 20 30 40 50 60 MIRRAPTTLQ LSHDDVTSLI DDLNEQKLKQ QLNIEKTKYF QGKNGGSLHS NTDFQDTSQN 70 80 90 100 110 120 IEDNNNDNDN DIDEDDDMSS YNDKAASVAH TRVLNSLHLS TDSNTAHETS NANDNHNPFY IREE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of yeast SCD26, a dosage-dependent suppressor of the cell cycle mutation cdc26." Dascher C., Kuentzel H. Nucleic Acids Res. 17:10491-10491(1989) [PubMed: 2690018] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Fifteen open reading frames in a 30.8 kb region of the right arm of chromosome VI from Saccharomyces cerevisiae." Eki T., Naitou M., Hagiwara H., Abe M., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Shibata T., Watanabe K., Ono A., Yamazaki M., Tashiro H., Hanaoka F., Murakami Y. Yeast 12:177-190(1996) [PubMed: 8686381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The CDC26 gene of Saccharomyces cerevisiae is required for cell growth only at high temperature." Araki H., Awane K., Ogawa N., Oshima Y. Mol. Gen. Genet. 231:329-331(1992) [PubMed: 1736102] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 47-54. Strain: P-28-24C.
[7]	"Identification of subunits of the anaphase-promoting complex of Saccharomyces cerevisiae." Zachariae W., Shin T.H., Galova M., Obermaier B., Nasmyth K. Science 274:1201-1204(1996) [PubMed: 8895471] [Abstract] Cited for: SUBUNIT.
[8]	"Swm1/Apc13 is an evolutionarily conserved subunit of the anaphase-promoting complex stabilizing the association of Cdc16 and Cdc27." Schwickart M., Havlis J., Habermann B., Bogdanova A., Camasses A., Oelschlaegel T., Shevchenko A., Zachariae W. Mol. Cell. Biol. 24:3562-3576(2004) [PubMed: 15060174] [Abstract] Cited for: SUBUNIT.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17118 Genomic DNA. Translation: CAA34978.1. D50617 Genomic DNA. Translation: BAA09275.1. AY558473 Genomic DNA. Translation: AAS56799.1. S79900 Genomic DNA. Translation: AAB21293.1. BK006940 Genomic DNA. Translation: DAA12479.1.
PIR	COBY26. S56291.
RefSeq	NP_116694.1. NM_001180001.1.
3D structure databases
ProteinModelPortal	P14724.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-352N.
IntAct	P14724. 15 interactions.
MINT	MINT-514276.
STRING	P14724.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFR036W; YFR036W; YFR036W.
GeneID	850597.
KEGG	sce:YFR036W.
NMPDR	fig|4932.3.peg.2329.
Organism-specific databases
CYGD	YFR036w.
SGD	S000001932. CDC26.
Phylogenomic databases
eggNOG	fuNOG12908.
OrthoDB	EOG42RHJ8.
Gene expression databases
ArrayExpress	P14724.
Genevestigator	P14724.
GermOnline	YFR036W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR018860. APC_suCDC26. [Graphical view]
KO	K03359.
Pfam	PF10471. APC_CDC26. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	966457.

Entry information

Entry name

CDC26_YEAST

Accession

Primary (citable) accession number: P14724
Secondary accession number(s): D6VTR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	February 1, 1994
Last modified:	December 14, 2011
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program