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Protein

Dihydroflavonol-4-reductase

Gene

DFRA

Organism
Petunia hybrida (Petunia)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Cis-3,4-leucopelargonidin + NADP+ = (+)-dihydrokaempferol + NADPH.

Pathway: anthocyanin biosynthesis

This protein is involved in the pathway anthocyanin biosynthesis, which is part of Pigment biosynthesis.
View all proteins of this organism that are known to be involved in the pathway anthocyanin biosynthesis and in Pigment biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Flavonoid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00009.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroflavonol-4-reductase (EC:1.1.1.219)
Short name:
DFR
Alternative name(s):
Dihydrokaempferol 4-reductase
Gene namesi
Name:DFRA
Synonyms:AN6
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 380380Dihydroflavonol-4-reductasePRO_0000215571Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP14720.
SMRiP14720. Positions 17-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001509. Epimerase_deHydtase_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASEAVHAPS PPVAVPTVCV TGAAGFIGSW LVMRLLERGY NVHATVRDPE
60 70 80 90 100
NKKKVKHLLE LPKADTNLTL WKADLTVEGS FDEAIQGCQG VFHVATPMDF
110 120 130 140 150
ESKDPENEVI KPTVRGMLSI IESCAKANTV KRLVFTSSAG TLDVQEQQKL
160 170 180 190 200
FYDQTSWSDL DFIYAKKMTG WMYFASKILA EKAAMEEAKK KNIDFISIIP
210 220 230 240 250
PLVVGPFITP TFPPSLITAL SLITGNEAHY CIIKQGQYVH LDDLCEAHIF
260 270 280 290 300
LYEHPKADGR FICSSHHAII YDVAKMVREK WPEYYVPTEF KGIDKDLPVV
310 320 330 340 350
SFSSKKLTDM GFQFKYTLED MYKGAIDTCR QKQLLPFSTR SAEDNGHNRE
360 370 380
AIAISAQNYA SGKENAPVAN HTEMLSNVEV
Length:380
Mass (Da):42,435
Last modified:October 1, 1996 - v2
Checksum:i6D7138AD5126868A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1616MASEA…PVAVP → MPLHLRCSA (PubMed:2491667).CuratedAdd
BLAST
Sequence conflicti71 – 711W → L in CAA33544 (PubMed:2491667).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79723 Genomic DNA. Translation: CAA56160.1.
X15537 mRNA. Translation: CAA33544.1.
PIRiS07463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X79723 Genomic DNA. Translation: CAA56160.1.
X15537 mRNA. Translation: CAA33544.1.
PIRiS07463.

3D structure databases

ProteinModelPortaliP14720.
SMRiP14720. Positions 17-338.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00009.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001509. Epimerase_deHydtase_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genetic control of dihydroflavonol 4-reductase gene expression in Petunia hybrida."
    Huits H., Gerats A.G.M., Kreike M.M., Mol J.N., Koes R.E.
    Plant J. 6:295-310(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Violet 30.
    Tissue: Leaf.
  2. "Flavonoid synthesis in Petunia hybrida: partial characterization of dihydroflavonol-4-reductase genes."
    Beld M., Martin C., Huits H., Stuitje A.R., Gerats A.G.M.
    Plant Mol. Biol. 13:491-502(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. R27.

Entry informationi

Entry nameiDFRA_PETHY
AccessioniPrimary (citable) accession number: P14720
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.