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P14720 (DFRA_PETHY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydroflavonol-4-reductase

Short name=DFR
EC=1.1.1.219
Alternative name(s):
Dihydrokaempferol 4-reductase
Gene names
Name:DFRA
Synonyms:AN6
OrganismPetunia hybrida (Petunia)
Taxonomic identifier4102 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

Protein attributes

Sequence length380 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Cis-3,4-leucopelargonidin + NADP+ = (+)-dihydrokaempferol + NADPH.

Pathway

Pigment biosynthesis; anthocyanin biosynthesis.

Sequence similarities

Belongs to the NAD(P)-dependent epimerase/dehydratase family. Dihydroflavonol-4-reductase subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 380380Dihydroflavonol-4-reductase
PRO_0000215571

Experimental info

Sequence conflict1 – 1616MASEA…PVAVP → MPLHLRCSA Ref.2
Sequence conflict711W → L in CAA33544. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P14720 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 6D7138AD5126868A

FASTA38042,435
        10         20         30         40         50         60 
MASEAVHAPS PPVAVPTVCV TGAAGFIGSW LVMRLLERGY NVHATVRDPE NKKKVKHLLE 

        70         80         90        100        110        120 
LPKADTNLTL WKADLTVEGS FDEAIQGCQG VFHVATPMDF ESKDPENEVI KPTVRGMLSI 

       130        140        150        160        170        180 
IESCAKANTV KRLVFTSSAG TLDVQEQQKL FYDQTSWSDL DFIYAKKMTG WMYFASKILA 

       190        200        210        220        230        240 
EKAAMEEAKK KNIDFISIIP PLVVGPFITP TFPPSLITAL SLITGNEAHY CIIKQGQYVH 

       250        260        270        280        290        300 
LDDLCEAHIF LYEHPKADGR FICSSHHAII YDVAKMVREK WPEYYVPTEF KGIDKDLPVV 

       310        320        330        340        350        360 
SFSSKKLTDM GFQFKYTLED MYKGAIDTCR QKQLLPFSTR SAEDNGHNRE AIAISAQNYA 

       370        380 
SGKENAPVAN HTEMLSNVEV 

« Hide

References

[1]"Genetic control of dihydroflavonol 4-reductase gene expression in Petunia hybrida."
Huits H., Gerats A.G.M., Kreike M.M., Mol J.N., Koes R.E.
Plant J. 6:295-310(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Violet 30.
Tissue: Leaf.
[2]"Flavonoid synthesis in Petunia hybrida: partial characterization of dihydroflavonol-4-reductase genes."
Beld M., Martin C., Huits H., Stuitje A.R., Gerats A.G.M.
Plant Mol. Biol. 13:491-502(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. R27.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79723 Genomic DNA. Translation: CAA56160.1.
X15537 mRNA. Translation: CAA33544.1.
PIRS07463.

3D structure databases

ProteinModelPortalP14720.
SMRP14720. Positions 17-338.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00009.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDFRA_PETHY
AccessionPrimary (citable) accession number: P14720
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways