P14698 (UCRI_NOSSP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b6-f complex iron-sulfur subunit
EC=1.10.9.1

Alternative name(s):
Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
Short name=ISP
Short name=RISP
Rieske iron-sulfur protein

Gene names

Name:

petC

Organism

Nostoc sp. (strain PCC 7906)

Taxonomic identifier

1181 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc › Nostoc muscorum

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity. HAMAP-Rule MF_01335
Catalytic activity	Plastoquinol + 2 oxidized plastocyanin + 2 H+(Side 1) = plastoquinone + 2 reduced plastocyanin + 2 H+(Side 2). HAMAP-Rule MF_01335
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. HAMAP-Rule MF_01335
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and PetN. The complex functions as a dimer By similarity.
Subcellular location	Cellular thylakoid membrane; Single-pass membrane protein By similarity. Note: The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity. HAMAP-Rule MF_01335
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein. HAMAP-Rule MF_01335
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Thylakoid
Domain	Transmembrane Transmembrane helix
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol--plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Cytochrome b6-f complex iron-sulfur subunit HAMAP-Rule MF_01335		PRO_0000127776
Regions
	Transmembrane	21 – 43	23	Helical; Potential
	Domain	61 – 162	102	Rieske
Sites
	Metal binding	108	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	110	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	126	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	129	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	113 ↔ 128		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14698 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: B5BCBFD8921BED85	FASTA	179	19,203
	10 20 30 40 50 60 MAQFSESADV PDMGRRQFMN LLTFGTVTGV ALGALYPVVK YFIPPASGGA GGGTTAKDEL 70 80 90 100 110 120 GNDVSLSKFL ENRNAGDRAL VQGLKGDPTY IVVENKQAIK DYGINAICTH LGCVVPWNVA 130 140 150 160 170 ENKFKCPCHG SQYDETGKVV RGPAPLSLAL AHANTVDDKI ILSPWTETDF RTGDAPWWA

References

[1]	"Primary structure of cotranscribed genes encoding the Rieske Fe-S and cytochrome f proteins of the cyanobacterium Nostoc PCC 7906." Kallas T., Spiller S., Malkin R. Proc. Natl. Acad. Sci. U.S.A. 85:5794-5798(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03855 Genomic DNA. Translation: AAA23332.1.
3D structure databases
ProteinModelPortal	P14698.
SMR	P14698. Positions 9-178.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.102.10.10. 1 hit.
HAMAP	MF_01335. Cytb6_f_Rieske.
InterPro	IPR014909. Cyt_b6-f_cplx_Fe-S_su. IPR023960. Cyt_b6_f_Rieske. IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. [Graphical view]
PANTHER	PTHR10134. PTHR10134. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
SUPFAM	SSF50022. Rieske_dom. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_NOSSP

Accession

Primary (citable) accession number: P14698

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program