P14697 (PHBB_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetoacetyl-CoA reductase
EC=1.1.1.36

Gene names

Name:	phbB
Ordered Locus Names:	H16_A1439

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-3-hydroxyacyl-CoA + NADP+ = 3-oxoacyl-CoA + NADPH.
Pathway	Biopolymer metabolism; poly-(R)-3-hydroxybutanoate biosynthesis.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	PHB biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	poly-hydroxybutyrate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetoacetyl-CoA reductase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Acetoacetyl-CoA reductase		PRO_0000054748
Regions
	Nucleotide binding	8 – 32	25	NADP By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14697 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: AD6739E0F5C93C06	FASTA	246	26,370
	10 20 30 40 50 60 MTQRIAYVTG GMGGIGTAIC QRLAKDGFRV VAGCGPNSPR REKWLEQQKA LGFDFIASEG 70 80 90 100 110 120 NVADWDSTKT AFDKVKSEVG EVDVLINNAG ITRDVVFRKM TRADWDAVID TNLTSLFNVT 130 140 150 160 170 180 KQVIDGMADR GWGRIVNISS VNGQKGQFGQ TNYSTAKAGL HGFTMALAQE VATKGVTVNT 190 200 210 220 230 240 VSPGYIATDM VKAIRQDVLD KIVATIPVKR LGLPEEIASI CAWLSSEESG FSTGADFSLN GGLHMG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase." Peoples O.P., Sinskey A.J. J. Biol. Chem. 264:15293-15297(1989) [PubMed: 2670935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04987 Genomic DNA. Translation: AAA21973.1. AM260479 Genomic DNA. Translation: CAJ92574.1.
PIR	RDALAE. B34340.
RefSeq	YP_725942.1. NC_008313.1.
3D structure databases
ProteinModelPortal	P14697.
ModBase	Search...
Protein-protein interaction databases
STRING	P14697.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4249784.
GenomeReviews	Gene locus H16_A1439 in contig AM260479_GR.
KEGG	reh:H16_A1439.
PATRIC	35232343. VBIRalEut6770_1828.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1028.
HOGENOM	HBG750976.
OMA	KMTRADW.
ProtClustDB	PRK12938.
Enzyme and pathway databases
BioCyc	REUT381666:H16_A1439-MONOMER.
Family and domain databases
InterPro	IPR011283. Acetoacetyl-CoA_reductase. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00023.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01829. AcAcCoA_reduct. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHBB_CUPNH

Accession

Primary (citable) accession number: P14697
Secondary accession number(s): Q0KBP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	January 25, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program