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Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Lactobacillus casei
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.UniRule annotation

Pathway: lactose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG)
This subpathway is part of the pathway lactose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate, the pathway lactose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei160 – 1601Proton donorUniRule annotation
Active sitei375 – 3751NucleophileUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKP14696.
UniPathwayiUPA00542; UER00605.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidaseUniRule annotation (EC:3.2.1.85UniRule annotation)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolaseUniRule annotation
Short name:
PGALaseUniRule annotation
P-beta-GalUniRule annotation
Short name:
PBGUniRule annotation
Gene namesi
Name:lacGUniRule annotation
OrganismiLactobacillus casei
Taxonomic identifieri1582 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4744746-phospho-beta-galactosidasePRO_0000063882Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi543734.LCABL_07280.

Structurei

3D structure databases

ProteinModelPortaliP14696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.UniRule annotation

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQLPQDFV MGGATAAYQV EGATKEDGKG RVLWDDFLDK QGRFKPDPAA
60 70 80 90 100
DFYHRYDEDL ALAEKYGHQV IRVSIAWSRI FPDGAGEVEP RGVAFYHKLF
110 120 130 140 150
ADCAAHHIEP FVTLHHFDTP ERLHEAGDWL SQEMLDDFVA YAKFCFEEFS
160 170 180 190 200
EVKYWITINE PTSMAVQQYT TGTFPPAESG RFDKTFQAEH NQMVAHARIV
210 220 230 240 250
NLYKSMQLGG QIGIVHALQT VYPYSDSAVD HHAAELQDAL ENRLYLDGTL
260 270 280 290 300
AGEYHQETLA LVKEILDANH QPMFQSTPQE MKAIDEAAHQ LDFVGVNNYF
310 320 330 340 350
SKWLRAYHGK SETIHNGDGT KGSSVARLQG VGEEKLPDGI ETTDWDWSIY
360 370 380 390 400
PRGMYDILMR IHNDYPLVPV TYVTENGIGL KESLPENATP DTVIEDPKRI
410 420 430 440 450
DYVKKYLSAM ADAIHDGANV KGYFIWSLQD QFSWTNGYSK RYGLFFVDFP
460 470
TQNRYIKQSA EWFKSVSETH IIPD
Length:474
Mass (Da):53,982
Last modified:April 1, 1990 - v1
Checksum:i1FF1B185E3549A9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20151 Genomic DNA. Translation: AAD15134.1.
Z80834 Genomic DNA. Translation: CAB02557.1.
M20150 Genomic DNA. Translation: AAA25238.1. Sequence problems.
PIRiA29897.
A29898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20151 Genomic DNA. Translation: AAD15134.1.
Z80834 Genomic DNA. Translation: CAB02557.1.
M20150 Genomic DNA. Translation: AAA25238.1. Sequence problems.
PIRiA29897.
A29898.

3D structure databases

ProteinModelPortaliP14696.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi543734.LCABL_07280.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00542; UER00605.
SABIO-RKP14696.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
HAMAPiMF_01574. LacG.
InterProiIPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the beta-D-phosphogalactoside galactohydrolase gene of Lactobacillus casei: comparison to analogous pbg genes of other Gram-positive organisms."
    Porter E.V., Chassy B.M.
    Gene 62:263-276(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Establishing a model to study the regulation of the lactose operon in Lactobacillus casei."
    Gosalbes M.J., Monedero V., Alpert C.-A., Perez-Martinez G.
    FEMS Microbiol. Lett. 148:83-89(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 393 / DSM 20011 / JCM 1134 / NBRC 15883 / NCIMB 11970 / NCDO 161.
  3. "Molecular cloning and nucleotide sequence of the factor IIIlac gene of Lactobacillus casei."
    Alpert C.-A., Chassy B.M.
    Gene 62:277-288(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 384-474.

Entry informationi

Entry nameiLACG_LACCA
AccessioniPrimary (citable) accession number: P14696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 24, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.