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Protein

6-phospho-beta-galactosidase

Gene

lacG

Organism
Lactobacillus casei
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol.UniRule annotation

Pathwayi: lactose degradation

This protein is involved in step 1 of the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 6-phospho-beta-galactosidase (AAW28_13990), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG), 6-phospho-beta-galactosidase (lacG)
This subpathway is part of the pathway lactose degradation, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate, the pathway lactose degradation and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei160Proton donorUniRule annotation1
Active sitei375NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

SABIO-RKiP14696.
UniPathwayiUPA00542; UER00605.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-galactosidaseUniRule annotation (EC:3.2.1.85UniRule annotation)
Alternative name(s):
Beta-D-phosphogalactoside galactohydrolaseUniRule annotation
Short name:
PGALaseUniRule annotation
P-beta-GalUniRule annotation
Short name:
PBGUniRule annotation
Gene namesi
Name:lacGUniRule annotation
OrganismiLactobacillus casei
Taxonomic identifieri1582 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638821 – 4746-phospho-beta-galactosidaseAdd BLAST474

Structurei

3D structure databases

ProteinModelPortaliP14696.
SMRiP14696.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CS2. Bacteria.
COG2723. LUCA.

Family and domain databases

HAMAPiMF_01574. LacG. 1 hit.
InterProiView protein in InterPro
IPR005928. 6P-beta-galactosidase.
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR017853. Glycoside_hydrolase_SF.
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiView protein in Pfam
PF00232. Glyco_hydro_1. 1 hit.
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01233. lacG. 1 hit.
PROSITEiView protein in PROSITE
PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P14696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQLPQDFV MGGATAAYQV EGATKEDGKG RVLWDDFLDK QGRFKPDPAA
60 70 80 90 100
DFYHRYDEDL ALAEKYGHQV IRVSIAWSRI FPDGAGEVEP RGVAFYHKLF
110 120 130 140 150
ADCAAHHIEP FVTLHHFDTP ERLHEAGDWL SQEMLDDFVA YAKFCFEEFS
160 170 180 190 200
EVKYWITINE PTSMAVQQYT TGTFPPAESG RFDKTFQAEH NQMVAHARIV
210 220 230 240 250
NLYKSMQLGG QIGIVHALQT VYPYSDSAVD HHAAELQDAL ENRLYLDGTL
260 270 280 290 300
AGEYHQETLA LVKEILDANH QPMFQSTPQE MKAIDEAAHQ LDFVGVNNYF
310 320 330 340 350
SKWLRAYHGK SETIHNGDGT KGSSVARLQG VGEEKLPDGI ETTDWDWSIY
360 370 380 390 400
PRGMYDILMR IHNDYPLVPV TYVTENGIGL KESLPENATP DTVIEDPKRI
410 420 430 440 450
DYVKKYLSAM ADAIHDGANV KGYFIWSLQD QFSWTNGYSK RYGLFFVDFP
460 470
TQNRYIKQSA EWFKSVSETH IIPD
Length:474
Mass (Da):53,982
Last modified:April 1, 1990 - v1
Checksum:i1FF1B185E3549A9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20151 Genomic DNA. Translation: AAD15134.1.
Z80834 Genomic DNA. Translation: CAB02557.1.
M20150 Genomic DNA. Translation: AAA25238.1. Sequence problems.
PIRiA29897.
A29898.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiLACG_LACCA
AccessioniPrimary (citable) accession number: P14696
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: July 5, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families