P14693 (SAM35_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sorting assembly machinery 35 kDa subunit

Alternative name(s):
Mitochondrial 38 kDa outer membrane protein
TOB complex 38 kDa subunit

Gene names

Name:	SAM35
Synonyms:	OMP85, TOB38, TOM38
Ordered Locus Names:	YHR083W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex. Ref.6 Ref.8
Subunit structure	Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37 and SAM50.
Subcellular location	Mitochondrion outer membrane Ref.6 Ref.7.
Miscellaneous	Present with 1470 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mitochondrial outer membrane translocase complex assembly Inferred from mutant phenotype Ref.8Ref.5. Source: SGD protein import into mitochondrial outer membrane Inferred from mutant phenotype Ref.8Ref.7. Source: SGD
Cellular component	mitochondrial sorting and assembly machinery complex Inferred from direct assay Ref.8Ref.5. Source: SGD
Molecular function	protein binding Inferred from physical interaction Ref.5. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SAM50	P53969	3	EBI-24602,EBI-28646

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Sorting assembly machinery 35 kDa subunit		PRO_0000202903

Sequences

	Sequence		Length	Mass (Da)
	P14693 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 4DCA565B56F35E4D	FASTA	329	37,404
	10 20 30 40 50 60 MVSSFSVPMP VKRIFDTFPL QTYAAQTDKD EAVALEIQRR SYTFTERGGG SSELTVEGTY 70 80 90 100 110 120 KLGVYNVFLE ANTGAALATD PWCLFVQLAL CQKNGLVLPT HSQEQTPSHT CNHEMLVLSR 130 140 150 160 170 180 LSNPDEALPI LVEGYKKRII RSTVAISEIM RSRILDDAEQ LMYYTLLDTV LYDCWITQII 190 200 210 220 230 240 FCASDAQFME LYSCQKLSGS IVTPLDVENS LLQKLSAKSL KISLTKRNKF QFRHREIVKS 250 260 270 280 290 300 MQGVYHNHHN SVNQEQVLNV LFENSKQVLL GLKDMLKSDG QPTYLHLKIA SYILCITNVK 310 320 EPIKLKTFVE NECKELVQFA QDTLKNFVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"STE12, a protein involved in cell-type-specific transcription and signal transduction in yeast, is part of protein-DNA complexes." Errede B., Ammerer G. Genes Dev. 3:1349-1361(1989) [PubMed: 2558054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 142-329.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Tob38, a novel essential component in the biogenesis of beta-barrel proteins of mitochondria." Waizenegger T., Habib S.J., Lech M., Mokranjac D., Paschen S.A., Hell K., Neupert W., Rapaport D. EMBO Rep. 5:704-709(2004) [PubMed: 15205677] [Abstract] Cited for: IDENTIFICATION IN THE SAM COMPLEX.
[6]	"The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria." Gentle I., Gabriel K., Beech P., Waller R., Lithgow T. J. Cell Biol. 164:19-24(2004) [PubMed: 14699090] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Two novel proteins in the mitochondrial outer membrane mediate beta-barrel protein assembly." Ishikawa D., Yamamoto H., Tamura Y., Moritoh K., Endo T. J. Cell Biol. 166:621-627(2004) [PubMed: 15326197] [Abstract] Cited for: IDENTIFICATION IN THE SAM COMPLEX, SUBCELLULAR LOCATION.
[8]	"Sam35 of the mitochondrial protein sorting and assembly machinery is a peripheral outer membrane protein essential for cell viability." Milenkovic D., Kozjak V., Wiedemann N., Lohaus C., Meyer H.E., Guiard B., Pfanner N., Meisinger C. J. Biol. Chem. 279:22781-22785(2004) [PubMed: 15067005] [Abstract] Cited for: FUNCTION, IDENTIFICATION IN THE SAM COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10556 Genomic DNA. Translation: AAB68885.1. X16112 Genomic DNA. Translation: CAA34245.1. BK006934 Genomic DNA. Translation: DAA06778.1.
PIR	S46807.
RefSeq	NP_011951.1. NM_001179213.1.
3D structure databases
ProteinModelPortal	P14693.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1879N.
IntAct	P14693. 97 interactions.
MINT	MINT-391230.
STRING	P14693.
Protein family/group databases
TCDB	1.B.33.3.1. outer membrane protein insertion porin (Bam Complex) (OmpIP) family.
Proteomic databases
PeptideAtlas	P14693.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YHR083W; YHR083W; YHR083W.
GeneID	856483.
KEGG	sce:YHR083W.
NMPDR	fig|4932.3.peg.3111.
Organism-specific databases
CYGD	YHR083w.
SGD	S000001125. SAM35.
Phylogenomic databases
eggNOG	fuNOG11884.
OMA	HINERET.
OrthoDB	EOG45XC5Q.
Gene expression databases
ArrayExpress	P14693.
Genevestigator	P14693.
GermOnline	YHR083W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021211. DUF2731. [Graphical view]
Pfam	PF10806. DUF2731. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	982173.

Entry information

Entry name

SAM35_YEAST

Accession

Primary (citable) accession number: P14693
Secondary accession number(s): D3DL34

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program