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Protein

Sorting assembly machinery 35 kDa subunit

Gene

SAM35

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex.2 Publications

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • cristae formation Source: Reactome
  • mitochondrial outer membrane translocase complex assembly Source: SGD
  • protein import into mitochondrial outer membrane Source: SGD
  • protein targeting to mitochondrion Source: Reactome

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31130-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.
R-SCE-8949613. Cristae formation.

Protein family/group databases

TCDBi1.B.33.3.1. the outer membrane protein insertion porin (bam complex) (ompip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting assembly machinery 35 kDa subunit
Alternative name(s):
Mitochondrial 38 kDa outer membrane protein
TOB complex 38 kDa subunit
Gene namesi
Name:SAM35
Synonyms:OMP85, TOB38, TOM38
Ordered Locus Names:YHR083W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR083W.
SGDiS000001125. SAM35.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002029031 – 329Sorting assembly machinery 35 kDa subunitAdd BLAST329

Proteomic databases

MaxQBiP14693.
PRIDEiP14693.

PTM databases

iPTMnetiP14693.

Interactioni

Subunit structurei

Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37 and SAM50.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36518. 86 interactors.
DIPiDIP-1879N.
IntActiP14693. 100 interactors.
MINTiMINT-391230.
STRINGi4932.YHR083W.

Structurei

3D structure databases

ProteinModelPortaliP14693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000000881.
InParanoidiP14693.
KOiK17715.
OMAiYDCWITQ.
OrthoDBiEOG092C4X01.

Family and domain databases

InterProiView protein in InterPro
IPR021211. SAM35.
PfamiView protein in Pfam
PF10806. SAM35. 1 hit.

Sequencei

Sequence statusi: Complete.

P14693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSSFSVPMP VKRIFDTFPL QTYAAQTDKD EAVALEIQRR SYTFTERGGG
60 70 80 90 100
SSELTVEGTY KLGVYNVFLE ANTGAALATD PWCLFVQLAL CQKNGLVLPT
110 120 130 140 150
HSQEQTPSHT CNHEMLVLSR LSNPDEALPI LVEGYKKRII RSTVAISEIM
160 170 180 190 200
RSRILDDAEQ LMYYTLLDTV LYDCWITQII FCASDAQFME LYSCQKLSGS
210 220 230 240 250
IVTPLDVENS LLQKLSAKSL KISLTKRNKF QFRHREIVKS MQGVYHNHHN
260 270 280 290 300
SVNQEQVLNV LFENSKQVLL GLKDMLKSDG QPTYLHLKIA SYILCITNVK
310 320
EPIKLKTFVE NECKELVQFA QDTLKNFVQ
Length:329
Mass (Da):37,404
Last modified:February 1, 1995 - v2
Checksum:i4DCA565B56F35E4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68885.1.
X16112 Genomic DNA. Translation: CAA34245.1.
BK006934 Genomic DNA. Translation: DAA06778.1.
PIRiS46807.
RefSeqiNP_011951.1. NM_001179213.1.

Genome annotation databases

EnsemblFungiiYHR083W; YHR083W; YHR083W.
GeneIDi856483.
KEGGisce:YHR083W.

Similar proteinsi

Entry informationi

Entry nameiSAM35_YEAST
AccessioniPrimary (citable) accession number: P14693
Secondary accession number(s): D3DL34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 1, 1995
Last modified: September 27, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names