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Protein

Sorting assembly machinery 35 kDa subunit

Gene

SAM35

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which is required for the sorting of proteins with complicated topology, such as beta-barrel proteins, to the mitochondrial outer membrane after import by the TOM complex.2 Publications

GO - Biological processi

  • mitochondrial outer membrane translocase complex assembly Source: SGD
  • protein import into mitochondrial outer membrane Source: SGD
  • protein targeting to mitochondrion Source: Reactome

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31130-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Protein family/group databases

TCDBi1.B.33.3.1. the outer membrane protein insertion porin (bam complex) (ompip) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting assembly machinery 35 kDa subunit
Alternative name(s):
Mitochondrial 38 kDa outer membrane protein
TOB complex 38 kDa subunit
Gene namesi
Name:SAM35
Synonyms:OMP85, TOB38, TOM38
Ordered Locus Names:YHR083W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR083W.
SGDiS000001125. SAM35.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial outer membrane Source: SGD
  • mitochondrial sorting and assembly machinery complex Source: SGD

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002029031 – 329Sorting assembly machinery 35 kDa subunitAdd BLAST329

Proteomic databases

MaxQBiP14693.
PRIDEiP14693.

PTM databases

iPTMnetiP14693.

Interactioni

Subunit structurei

Component of the mitochondrial outer membrane sorting assembly machinery (SAM or TOB) complex, which at least consists of SAM35, SAM37 and SAM50.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36518. 86 interactors.
DIPiDIP-1879N.
IntActiP14693. 99 interactors.
MINTiMINT-391230.

Structurei

3D structure databases

ProteinModelPortaliP14693.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000000881.
InParanoidiP14693.
KOiK17715.
OMAiYDCWITQ.
OrthoDBiEOG092C4X01.

Family and domain databases

InterProiView protein in InterPro
IPR021211. SAM35.
PfamiView protein in Pfam
PF10806. SAM35. 1 hit.

Sequencei

Sequence statusi: Complete.

P14693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSSFSVPMP VKRIFDTFPL QTYAAQTDKD EAVALEIQRR SYTFTERGGG
60 70 80 90 100
SSELTVEGTY KLGVYNVFLE ANTGAALATD PWCLFVQLAL CQKNGLVLPT
110 120 130 140 150
HSQEQTPSHT CNHEMLVLSR LSNPDEALPI LVEGYKKRII RSTVAISEIM
160 170 180 190 200
RSRILDDAEQ LMYYTLLDTV LYDCWITQII FCASDAQFME LYSCQKLSGS
210 220 230 240 250
IVTPLDVENS LLQKLSAKSL KISLTKRNKF QFRHREIVKS MQGVYHNHHN
260 270 280 290 300
SVNQEQVLNV LFENSKQVLL GLKDMLKSDG QPTYLHLKIA SYILCITNVK
310 320
EPIKLKTFVE NECKELVQFA QDTLKNFVQ
Length:329
Mass (Da):37,404
Last modified:February 1, 1995 - v2
Checksum:i4DCA565B56F35E4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68885.1.
X16112 Genomic DNA. Translation: CAA34245.1.
BK006934 Genomic DNA. Translation: DAA06778.1.
PIRiS46807.
RefSeqiNP_011951.1. NM_001179213.1.

Genome annotation databases

EnsemblFungiiYHR083W; YHR083W; YHR083W.
GeneIDi856483.
KEGGisce:YHR083W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10556 Genomic DNA. Translation: AAB68885.1.
X16112 Genomic DNA. Translation: CAA34245.1.
BK006934 Genomic DNA. Translation: DAA06778.1.
PIRiS46807.
RefSeqiNP_011951.1. NM_001179213.1.

3D structure databases

ProteinModelPortaliP14693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36518. 86 interactors.
DIPiDIP-1879N.
IntActiP14693. 99 interactors.
MINTiMINT-391230.

Protein family/group databases

TCDBi1.B.33.3.1. the outer membrane protein insertion porin (bam complex) (ompip) family.

PTM databases

iPTMnetiP14693.

Proteomic databases

MaxQBiP14693.
PRIDEiP14693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR083W; YHR083W; YHR083W.
GeneIDi856483.
KEGGisce:YHR083W.

Organism-specific databases

EuPathDBiFungiDB:YHR083W.
SGDiS000001125. SAM35.

Phylogenomic databases

HOGENOMiHOG000000881.
InParanoidiP14693.
KOiK17715.
OMAiYDCWITQ.
OrthoDBiEOG092C4X01.

Enzyme and pathway databases

BioCyciYEAST:G3O-31130-MONOMER.
ReactomeiR-SCE-1268020. Mitochondrial protein import.

Miscellaneous databases

PROiP14693.

Family and domain databases

InterProiView protein in InterPro
IPR021211. SAM35.
PfamiView protein in Pfam
PF10806. SAM35. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSAM35_YEAST
AccessioniPrimary (citable) accession number: P14693
Secondary accession number(s): D3DL34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: February 1, 1995
Last modified: March 15, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.