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Protein

26S proteasome non-ATPase regulatory subunit 3

Gene

Psmd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTumor antigen

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-446652. Interleukin-1 family signaling.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-5689603. UCH proteinases.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.
R-MMU-8941858. Regulation of RUNX3 expression and activity.
R-MMU-8948751. Regulation of PTEN stability and activity.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 3
Alternative name(s):
26S proteasome regulatory subunit RPN3
26S proteasome regulatory subunit S3
Proteasome subunit p58
Transplantation antigen P91A
Tum-P91A antigen
Gene namesi
Name:Psmd3
Synonyms:P91a, Tstap91a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:98858. Psmd3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738171 – 53026S proteasome non-ATPase regulatory subunit 3Add BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki36Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki36Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei414PhosphoserineBy similarity1
Modified residuei426PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP14685.
MaxQBiP14685.
PaxDbiP14685.
PeptideAtlasiP14685.
PRIDEiP14685.

PTM databases

iPTMnetiP14685.
PhosphoSitePlusiP14685.
SwissPalmiP14685.

Expressioni

Gene expression databases

BgeeiENSMUSG00000017221.
ExpressionAtlasiP14685. baseline and differential.
GenevisibleiP14685. MM.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD3, a base containing 6 ATPases and few additional components. Interacts with UBQLN1 (via ubiquitin-like domain).By similarity1 Publication

Protein-protein interaction databases

BioGridi204358. 4 interactors.
IntActiP14685. 6 interactors.
STRINGi10090.ENSMUSP00000017365.

Structurei

3D structure databases

ProteinModelPortaliP14685.
SMRiP14685.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini354 – 458PCIAdd BLAST105

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi25 – 30Poly-Pro6

Sequence similaritiesi

Belongs to the proteasome subunit S3 family.Curated

Phylogenomic databases

eggNOGiKOG2581. Eukaryota.
ENOG410XS40. LUCA.
GeneTreeiENSGT00490000043406.
HOGENOMiHOG000193909.
HOVERGENiHBG000703.
InParanoidiP14685.
KOiK03033.
OMAiAMRYPPK.
OrthoDBiEOG091G05QN.
TreeFamiTF106110.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.25.40.10. 1 hit.
InterProiView protein in InterPro
IPR013586. 26S_Psome_reg_C.
IPR000717. PCI_dom.
IPR035267. PSMD3.
IPR011990. TPR-like_helical_dom.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR10758:SF6. PTHR10758:SF6. 1 hit.
PfamiView protein in Pfam
PF01399. PCI. 1 hit.
PF08375. Rpn3_C. 1 hit.
SMARTiView protein in SMART
SM00088. PINT. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequencei

Sequence statusi: Complete.

P14685-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQEGSARRR GADKAKPPPG GEQEPPPPAP QDVEMKEEAA AGSGSTGEGD
60 70 80 90 100
GKAAATEHSQ RELDTVTLED IKEHVRQLEK AVSGKEPRFV LRALRMLPST
110 120 130 140 150
SRRLNHYVLY KAVHGFFTSN NATRDFLLPF LEEPMDTEAD LQFRPRTGKA
160 170 180 190 200
ASAPLLPEVE AYLQLLMVIF LMNSKRYKEA QKISDDLMQK ISTQNRRALD
210 220 230 240 250
LVAAKCYYYH ARVYEFLDKL DVVRSFLHAR LRTATLRHDA DGQATLLNLL
260 270 280 290 300
LRNYLHYSLY DQAEKLVSKS VFPEQANNNE WARYLYYTGR IKAIQLEYSE
310 320 330 340 350
ARRTMTNALR KAPQHTAVGF KQTVHKLLIV VELLLGEIPD RLQFRQPSLK
360 370 380 390 400
RSLMPYFLLT QAVRTGNLAK FNQVLDQFGE KFQTDGTYTL IIRLRHNVIK
410 420 430 440 450
TGVRMISLSY SRISLADIAQ KLQLDSPEDA EFIVAKAIRD GVIEASINHE
460 470 480 490 500
KGYVQSKEMI DIYSTREPQL AFHQRISFCL DIHNMSVKAM RFPPKSYNKD
510 520 530
LESAEERRER EQQDLEFAKE MAEDDDDSFP
Length:530
Mass (Da):60,718
Last modified:July 27, 2011 - v3
Checksum:iCAB9C9AA3A0EEC8D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12 – 13AD → H in AAA37241 (PubMed:2568889).Curated2
Sequence conflicti196R → H in AAA37241 (PubMed:2568889).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25149 Genomic DNA. Translation: AAA37241.1.
AK145488 mRNA. Translation: BAE26466.1.
AK164593 mRNA. Translation: BAE37842.1.
AK165520 mRNA. Translation: BAE38235.1.
AK166683 mRNA. Translation: BAE38942.1.
AK166714 mRNA. Translation: BAE38965.1.
AK167285 mRNA. Translation: BAE39391.1.
AK169690 mRNA. Translation: BAE41307.1.
AL590963 Genomic DNA. Translation: CAM46197.1.
CH466556 Genomic DNA. Translation: EDL16159.1.
BC003197 mRNA. Translation: AAH03197.1.
CCDSiCCDS25359.1.
PIRiI49504.
RefSeqiNP_033465.1. NM_009439.1.
UniGeneiMm.12194.

Genome annotation databases

EnsembliENSMUST00000017365; ENSMUSP00000017365; ENSMUSG00000017221.
GeneIDi22123.
KEGGimmu:22123.
UCSCiuc007lgw.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiPSMD3_MOUSE
AccessioniPrimary (citable) accession number: P14685
Secondary accession number(s): Q3TP95, Q99LL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: July 27, 2011
Last modified: September 27, 2017
This is version 147 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families