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Protein

Ubiquitin-conjugating enzyme E2-34 kDa

Gene

CDC34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Capable, in vitro, to ubiquitinate histone H2A.
Mediates the initiation of DNA replication (transition of G1 to S phase in cell cycle). Essential component of the E3 ubiquitin ligase complex SCF (SKP1-CUL1-F-box protein), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Involved in the regulation of methionine biosynthesis genes and in the degradation of CDC6 together with CDC4 and CDC53.

Miscellaneous

Present with 8170 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein homodimerization activity Source: SGD
  • ubiquitin protein ligase binding Source: GO_Central
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • DNA replication Source: UniProtKB-KW
  • G1/S transition of mitotic cell cycle Source: SGD
  • G2/M transition of mitotic cell cycle Source: SGD
  • protein autoubiquitination Source: SGD
  • protein polyubiquitination Source: SGD
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Cell division, DNA replication, Ubl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29663-MONOMER
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-34 kDa (EC:2.3.2.23)
Alternative name(s):
Cell division control protein 34
E2 ubiquitin-conjugating enzyme 3
E3 ubiquitin ligase complex SCF subunit CDC34
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:CDC34
Synonyms:DNA6, UBC3
Ordered Locus Names:YDR054C
ORF Names:D4211, YD9609.08C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR054C
SGDiS000002461 CDC34

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825411 – 295Ubiquitin-conjugating enzyme E2-34 kDaAdd BLAST295

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186PhosphoserineCombined sources1
Modified residuei282PhosphoserineCombined sources1
Modified residuei292PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP14682
PaxDbiP14682
PRIDEiP14682

PTM databases

iPTMnetiP14682

Interactioni

Subunit structurei

Interacts with CDC53. Component of the E3 ubiquitin ligase complexes SCF with CDC53, SKP1/CBF3D, HRT1 and some F-box proteins like MET30 and CDC4.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC53Q120183EBI-19730,EBI-4321

GO - Molecular functioni

  • protein homodimerization activity Source: SGD
  • ubiquitin protein ligase binding Source: GO_Central

Protein-protein interaction databases

BioGridi32107, 448 interactors
DIPiDIP-1618N
IntActiP14682, 6 interactors
MINTiP14682
STRINGi4932.YDR054C

Structurei

3D structure databases

ProteinModelPortaliP14682
SMRiP14682
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi191 – 289Asp/Glu-rich (acidic)Add BLAST99

Domaini

The acidic C-terminal extension is essential for the cell cycle function.

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00910000146625
HOGENOMiHOG000233454
InParanoidiP14682
KOiK02207
OMAiFKATIRF
OrthoDBiEOG092C50OL

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P14682-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRKSTASS LLLRQYRELT DPKKAIPSFH IELEDDSNIF TWNIGVMVLN
60 70 80 90 100
EDSIYHGGFF KAQMRFPEDF PFSPPQFRFT PAIYHPNVYR DGRLCISILH
110 120 130 140 150
QSGDPMTDEP DAETWSPVQT VESVLISIVS LLEDPNINSP ANVDAAVDYR
160 170 180 190 200
KNPEQYKQRV KMEVERSKQD IPKGFIMPTS ESAYISQSKL DEPESNKDMA
210 220 230 240 250
DNFWYDSDLD DDENGSVILQ DDDYDDGNNH IPFEDDDVYN YNDNDDDDER
260 270 280 290
IEFEDDDDDD DDSIDNDSVM DRKQPHKAED ESEDVEDVER VSKKI
Length:295
Mass (Da):34,065
Last modified:April 1, 1990 - v1
Checksum:i1CE3E0C3AB1436DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21877 Genomic DNA Translation: AAA35188.1
X84162 Genomic DNA Translation: CAA58970.1
Z74350 Genomic DNA Translation: CAA98872.1
Z49209 Genomic DNA Translation: CAA89083.1
BK006938 Genomic DNA Translation: DAA11900.1
PIRiA41241
RefSeqiNP_010339.1, NM_001180362.1

Genome annotation databases

EnsemblFungiiYDR054C; YDR054C; YDR054C
GeneIDi851624
KEGGisce:YDR054C

Similar proteinsi

Entry informationi

Entry nameiUBC3_YEAST
AccessioniPrimary (citable) accession number: P14682
Secondary accession number(s): D6VS40
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 23, 2018
This is version 189 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

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