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P14681

- KSS1_YEAST

UniProt

P14681 - KSS1_YEAST

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Protein

Mitogen-activated protein kinase KSS1

Gene

KSS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Together with closely related FUS3, KSS1 is the final kinase in the signal transduction cascade regulating activation/repression of the mating and filamentation pathways, induced by pheromone and nitrogen/carbon limitation, respectively. Phosphorylated KSS1 activates both pathways, whereas activated FUS3 activates the mating but suppresses the filamentation pathway. KSS1 activity is down-regulated by FUS3 during pheromone induction to prevent inappropriate activation of the filamentation pathway. During induction of filamentation, KSS1 activates the transcription factor STE12 resulting in its binding to and activation of filamentation specific genes. Non-activated KSS1 has a kinase-independent repressive effect on STE12 transcriptional activity, that is mediated by direct binding to STE12 and depends on the presence of DIG1 and DIG2, and that is required for the suppression of filamentation under normal growth conditions. SSN3/SRB10 contributes further to the suppression of filamentation under these conditions by reducing STE12 stability independent of KSS1. FUS3 can partially compensate for the lack of KSS1 but filamentation becomes constitutively induced at a low level in the absence of any signal. KSS1 phosphorylates STE7, STE5, FAR1, DIG1, DIG2, STE12, and SST2.8 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation after pheromone treatment or carbon/nitrogen limitation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATPPROSITE-ProRule annotation
Active sitei143 – 1431Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: SGD
  3. transcription factor binding Source: SGD

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. invasive growth in response to glucose limitation Source: SGD
  3. MAPK cascade Source: GOC
  4. negative regulation of sequence-specific DNA binding transcription factor activity Source: SGD
  5. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  6. protein phosphorylation Source: SGD
  7. signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30760-MONOMER.
BRENDAi2.7.11.24. 984.
ReactomeiREACT_191508. Signaling by FGFR.
REACT_205607. Regulation of HSF1-mediated heat shock response.
REACT_207653. Signal transduction by L1.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase KSS1 (EC:2.7.11.24)
Short name:
MAP kinase KSS1
Alternative name(s):
Kinase suppressor of SST2
Gene namesi
Name:KSS1
Ordered Locus Names:YGR040W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGR040w.
SGDiS000003272. KSS1.

Subcellular locationi

Nucleus 1 Publication. Cytoplasm 1 Publication. Periplasm 1 Publication
Note: KSS1 shuttles rapidly between the cytoplasm and the nucleus independent of its activation state.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: SGD
  3. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Mitogen-activated protein kinase KSS1PRO_0000186333Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831Phosphothreonine1 Publication
Modified residuei185 – 1851Phosphotyrosine1 Publication

Post-translational modificationi

Dually phosphorylated on Thr-183 and Tyr-185 by STE7 in response to pheromone or carbon/nitrogen limitation, which activates the enzyme. Activated FUS3 down-regulates KSS1 phosphorylation.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP14681.
PaxDbiP14681.
PeptideAtlasiP14681.

Expressioni

Gene expression databases

GenevestigatoriP14681.

Interactioni

Subunit structurei

In the nucleus, KSS1 forms a complex with DIG1, DIG2 and STE12; in contrast to FUS3 the interaction of KSS1 with STE12 does not depend on DIG1 and DIG2. Phosphorylated KSS1 shows reduced interaction with STE12. During pheromone activation and phosphorylation, KSS1 forms a membrane-associated complex with the scaffold protein STE5, the MAPKK STE7, the MAPKKK STE11, and the G-protein beta subunit GBB/STE4; interacting directly with POF1, STE7 and STE5 proteins.

Binary interactionsi

WithEntry#Exp.IntActNotes
BCK2P333064EBI-9945,EBI-3480
DIG1Q030633EBI-9945,EBI-29752
DIG2Q033733EBI-9945,EBI-34019
STE11P235614EBI-9945,EBI-18259
STE12P135746EBI-9945,EBI-18264
STE7P0678414EBI-9945,EBI-18389

Protein-protein interaction databases

BioGridi33287. 177 interactions.
DIPiDIP-60N.
IntActiP14681. 18 interactions.
MINTiMINT-411417.
STRINGi4932.YGR040W.

Structurei

3D structure databases

ProteinModelPortaliP14681.
SMRiP14681. Positions 1-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 313301Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi183 – 1853TXY

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi345 – 3495Poly-Glu

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily. HOG1 sub-subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074298.
HOGENOMiHOG000233024.
InParanoidiP14681.
KOiK04371.
OMAiGPRYTDL.
OrthoDBiEOG7K3TWD.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14681-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MARTITFDIP SQYKLVDLIG EGAYGTVCSA IHKPSGIKVA IKKIQPFSKK
60 70 80 90 100
LFVTRTIREI KLLRYFHEHE NIISILDKVR PVSIDKLNAV YLVEELMETD
110 120 130 140 150
LQKVINNQNS GFSTLSDDHV QYFTYQILRA LKSIHSAQVI HRDIKPSNLL
160 170 180 190 200
LNSNCDLKVC DFGLARCLAS SSDSRETLVG FMTEYVATRW YRAPEIMLTF
210 220 230 240 250
QEYTTAMDIW SCGCILAEMV SGKPLFPGRD YHHQLWLILE VLGTPSFEDF
260 270 280 290 300
NQIKSKRAKE YIANLPMRPP LPWETVWSKT DLNPDMIDLL DKMLQFNPDK
310 320 330 340 350
RISAAEALRH PYLAMYHDPS DEPEYPPLNL DDEFWKLDNK IMRPEEEEEV
360
PIEMLKDMLY DELMKTME
Length:368
Mass (Da):42,692
Last modified:April 1, 1990 - v1
Checksum:i5FA42161B5FD51B5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26398 Genomic DNA. Translation: AAA34882.1.
DQ115391 Genomic DNA. Translation: AAZ22456.1.
Z72825 Genomic DNA. Translation: CAA97038.1.
AY557773 Genomic DNA. Translation: AAS56099.1.
BK006941 Genomic DNA. Translation: DAA08139.1.
PIRiA33297.
RefSeqiNP_011554.3. NM_001181169.3.

Genome annotation databases

EnsemblFungiiYGR040W; YGR040W; YGR040W.
GeneIDi852931.
KEGGisce:YGR040W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M26398 Genomic DNA. Translation: AAA34882.1 .
DQ115391 Genomic DNA. Translation: AAZ22456.1 .
Z72825 Genomic DNA. Translation: CAA97038.1 .
AY557773 Genomic DNA. Translation: AAS56099.1 .
BK006941 Genomic DNA. Translation: DAA08139.1 .
PIRi A33297.
RefSeqi NP_011554.3. NM_001181169.3.

3D structure databases

ProteinModelPortali P14681.
SMRi P14681. Positions 1-364.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33287. 177 interactions.
DIPi DIP-60N.
IntActi P14681. 18 interactions.
MINTi MINT-411417.
STRINGi 4932.YGR040W.

Proteomic databases

MaxQBi P14681.
PaxDbi P14681.
PeptideAtlasi P14681.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGR040W ; YGR040W ; YGR040W .
GeneIDi 852931.
KEGGi sce:YGR040W.

Organism-specific databases

CYGDi YGR040w.
SGDi S000003272. KSS1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074298.
HOGENOMi HOG000233024.
InParanoidi P14681.
KOi K04371.
OMAi GPRYTDL.
OrthoDBi EOG7K3TWD.

Enzyme and pathway databases

BioCyci YEAST:G3O-30760-MONOMER.
BRENDAi 2.7.11.24. 984.
Reactomei REACT_191508. Signaling by FGFR.
REACT_205607. Regulation of HSF1-mediated heat shock response.
REACT_207653. Signal transduction by L1.

Miscellaneous databases

NextBioi 972657.
PROi P14681.

Gene expression databases

Genevestigatori P14681.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae."
    Courchesne W.E., Kunisawa R., Thorner J.
    Cell 58:1107-1119(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Quantitative trait loci mapped to single-nucleotide resolution in yeast."
    Deutschbauer A.M., Davis R.W.
    Nat. Genet. 37:1333-1340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SK1.
  3. "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII."
    Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.
    Yeast 13:1077-1090(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1."
    Gartner A., Nasmyth K., Ammerer G.
    Genes Dev. 6:1280-1292(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  8. "Two novel targets of the MAP kinase Kss1 are negative regulators of invasive growth in the yeast Saccharomyces cerevisiae."
    Cook J.G., Bardwell L., Kron S.J., Thorner J.
    Genes Dev. 10:2831-2848(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COMPLEX WITH DIG1; DIG2 AND STE12.
  9. "MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation."
    Madhani H.D., Styles C.A., Fink G.R.
    Cell 91:673-684(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INHIBITION OF FILAMENTATION.
  10. "Repression of yeast Ste12 transcription factor by direct binding of unphosphorylated Kss1 MAPK and its regulation by the Ste7 MEK."
    Bardwell L., Cook J.G., Voora D., Baggott D.M., Martinez A.R., Thorner J.
    Genes Dev. 12:2887-2898(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH STE12.
  11. "Differential regulation of transcription: repression by unactivated mitogen-activated protein kinase Kss1 requires the Dig1 and Dig2 proteins."
    Bardwell L., Cook J.G., Zhu-Shimoni J.X., Voora D., Thorner J.
    Proc. Natl. Acad. Sci. U.S.A. 95:15400-15405(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, FUNCTIONAL DEPENDENCE ON DIG1/DIG2.
  12. "Specificity of MAP kinase signaling in yeast differentiation involves transient versus sustained MAPK activation."
    Sabbagh W. Jr., Flatauer L.J., Bardwell A.J., Bardwell L.
    Mol. Cell 8:683-691(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULATION OF ACTIVITY BY FUS3.
  13. "MAP kinase dynamics in response to pheromones in budding yeast."
    van Drogen F., Stucke V.M., Jorritsma G., Peter M.
    Nat. Cell Biol. 3:1051-1059(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  14. "Program-specific distribution of a transcription factor dependent on partner transcription factor and MAPK signaling."
    Zeitlinger J., Simon I., Harbison C.T., Hannett N.M., Volkert T.L., Fink G.R., Young R.A.
    Cell 113:395-404(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGULATION OF STE12 PROMOTER SELECTIVITY.
  15. "Srb10/Cdk8 regulates yeast filamentous growth by phosphorylating the transcription factor Ste12."
    Nelson C., Goto S., Lund K., Hung W., Sadowski I.
    Nature 421:187-190(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, STE12 STABILITY.
  16. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  17. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-183 AND TYR-185, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  18. "Diverse protein kinase interactions identified by protein microarrays reveal novel connections between cellular processes."
    Fasolo J., Sboner A., Sun M.G., Yu H., Chen R., Sharon D., Kim P.M., Gerstein M., Snyder M.
    Genes Dev. 25:767-778(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH POF1.

Entry informationi

Entry nameiKSS1_YEAST
AccessioniPrimary (citable) accession number: P14681
Secondary accession number(s): D6VUH8, Q45U43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: October 29, 2014
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5480 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3