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P14677

- PBPX_STRPN

UniProt

P14677 - PBPX_STRPN

Protein

Penicillin-binding protein 2x

Gene

pbpX

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei337 – 3371Acyl-ester intermediateBy similarity

    GO - Molecular functioni

    1. penicillin binding Source: InterPro

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. peptidoglycan biosynthetic process Source: UniProtKB-KW
    4. regulation of cell shape Source: UniProtKB-KW
    5. response to antibiotic Source: UniProtKB-KW

    Keywords - Biological processi

    Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Enzyme and pathway databases

    BioCyciSPNE170187:GHGN-342-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Penicillin-binding protein 2x
    Short name:
    PBP-2x
    Short name:
    PBP2x
    Gene namesi
    Name:pbpX
    Ordered Locus Names:SP_0336
    OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
    Taxonomic identifieri170187 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000585: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 750750Penicillin-binding protein 2xPRO_0000195453Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi170187.SP_0336.

    Structurei

    Secondary structure

    1
    750
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi65 – 728
    Beta strandi86 – 916
    Beta strandi93 – 997
    Beta strandi107 – 1093
    Turni116 – 1183
    Helixi119 – 13012
    Helixi134 – 1418
    Beta strandi147 – 1504
    Helixi153 – 1553
    Beta strandi156 – 1583
    Helixi160 – 17011
    Turni171 – 1744
    Beta strandi177 – 1837
    Beta strandi185 – 1873
    Beta strandi191 – 1944
    Helixi195 – 1984
    Beta strandi201 – 2055
    Turni207 – 2093
    Beta strandi211 – 2155
    Helixi218 – 2225
    Helixi224 – 2285
    Beta strandi232 – 2398
    Beta strandi250 – 2534
    Beta strandi260 – 2645
    Helixi267 – 28418
    Beta strandi287 – 2959
    Turni296 – 2983
    Beta strandi300 – 3089
    Turni312 – 3143
    Turni327 – 3293
    Helixi336 – 3394
    Helixi340 – 34910
    Beta strandi358 – 3603
    Beta strandi364 – 3663
    Beta strandi369 – 3713
    Helixi374 – 3774
    Beta strandi378 – 3803
    Beta strandi385 – 3873
    Helixi388 – 3936
    Helixi397 – 40711
    Helixi409 – 41810
    Turni419 – 4224
    Beta strandi428 – 4314
    Helixi442 – 4476
    Helixi448 – 4503
    Helixi458 – 46912
    Turni470 – 4723
    Beta strandi480 – 4856
    Turni486 – 4894
    Beta strandi490 – 4934
    Beta strandi498 – 5003
    Helixi505 – 52016
    Turni522 – 5243
    Turni530 – 5323
    Beta strandi533 – 5375
    Beta strandi545 – 5528
    Helixi556 – 5583
    Beta strandi559 – 5613
    Beta strandi568 – 58013
    Beta strandi582 – 59110
    Helixi597 – 61721
    Helixi625 – 6284
    Helixi646 – 6549
    Turni655 – 6573
    Beta strandi659 – 6657
    Beta strandi667 – 6748
    Beta strandi685 – 6917
    Helixi704 – 71411
    Beta strandi717 – 73216
    Turni738 – 7403
    Beta strandi743 – 7497

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PMDX-ray3.50A76-750[»]
    1QMEX-ray2.40A49-750[»]
    1QMFX-ray2.80A49-750[»]
    1RP5X-ray3.00A/B49-750[»]
    2Z2LX-ray2.85A/D71-238[»]
    B/E241-625[»]
    C/F626-750[»]
    2Z2MX-ray2.60A/D71-238[»]
    B/E241-625[»]
    C/F626-750[»]
    2ZC3X-ray2.50A/D71-238[»]
    B/E241-625[»]
    C/F626-750[»]
    2ZC4X-ray2.80A/D71-238[»]
    B/E241-625[»]
    C/F626-750[»]
    ProteinModelPortaliP14677.
    SMRiP14677. Positions 64-750.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14677.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei29 – 4921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini632 – 69160PASTA 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini692 – 75059PASTA 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the transpeptidase family.Curated
    Contains 2 PASTA domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0768.
    HOGENOMiHOG000049551.
    KOiK12556.
    OMAiKMMKPYV.
    OrthoDBiEOG6N0HHV.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR005543. PASTA_dom.
    IPR005311. PBP_dimer.
    IPR001460. PCN-bd_Tpept.
    [Graphical view]
    PfamiPF03793. PASTA. 1 hit.
    PF03717. PBP_dimer. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SMARTiSM00740. PASTA. 1 hit.
    [Graphical view]
    SUPFAMiSSF56519. SSF56519. 1 hit.
    SSF56601. SSF56601. 1 hit.
    PROSITEiPS51178. PASTA. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14677-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKWTKRVIRY ATKNRKSPAE NRRRVGKSLS LLSVFVFAIF LVNFAVIIGT    50
    GTRFGTDLAK EAKKVHQTTR TVPAKRGTIY DRNGVPIAED ATSYNVYAVI 100
    DENYKSATGK ILYVEKTQFN KVAEVFHKYL DMEESYVREQ LSQPNLKQVS 150
    FGAKGNGITY ANMMSIKKEL EAAEVKGIDF TTSPNRSYPN GQFASSFIGL 200
    AQLHENEDGS KSLLGTSGME SSLNSILAGT DGIITYEKDR LGNIVPGTEQ 250
    VSQRTMDGKD VYTTISSPLQ SFMETQMDAF QEKVKGKYMT ATLVSAKTGE 300
    ILATTQRPTF DADTKEGITE DFVWRDILYQ SNYEPGSTMK VMMLAAAIDN 350
    NTFPGGEVFN SSELKIADAT IRDWDVNEGL TGGRTMTFSQ GFAHSSNVGM 400
    TLLEQKMGDA TWLDYLNRFK FGVPTRFGLT DEYAGQLPAD NIVNIAQSSF 450
    GQGISVTQTQ MIRAFTAIAN DGVMLEPKFI SAIYDPNDQT ARKSQKEIVG 500
    NPVSKDAASL TRTNMVLVGT DPVYGTMYNH STGKPTVTVP GQNVALKSGT 550
    AQIADEKNGG YLVGLTDYIF SAVSMSPAEN PDFILYVTVQ QPEHYSGIQL 600
    GEFANPILER ASAMKDSLNL QTTAKALEQV SQQSPYPMPS VKDISPGDLA 650
    EELRRNLVQP IVVGTGTKIK NSSAEEGKNL APNQQVLILS DKAEEVPDMY 700
    GWTKETAETL AKWLNIELEF QGSGSTVQKQ DVRANTAIKD IKKITLTLGD 750
    Length:750
    Mass (Da):82,313
    Last modified:September 26, 2001 - v2
    Checksum:i9B2BCADBE4E462E4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005672 Genomic DNA. Translation: AAK74511.1.
    PIRiF95039.
    H97909.
    S06726.
    RefSeqiNP_344871.1. NC_003028.3.

    Genome annotation databases

    EnsemblBacteriaiAAK74511; AAK74511; SP_0336.
    GeneIDi930153.
    KEGGispn:SP_0336.
    PATRICi19705009. VBIStrPne105772_0350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005672 Genomic DNA. Translation: AAK74511.1 .
    PIRi F95039.
    H97909.
    S06726.
    RefSeqi NP_344871.1. NC_003028.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PMD X-ray 3.50 A 76-750 [» ]
    1QME X-ray 2.40 A 49-750 [» ]
    1QMF X-ray 2.80 A 49-750 [» ]
    1RP5 X-ray 3.00 A/B 49-750 [» ]
    2Z2L X-ray 2.85 A/D 71-238 [» ]
    B/E 241-625 [» ]
    C/F 626-750 [» ]
    2Z2M X-ray 2.60 A/D 71-238 [» ]
    B/E 241-625 [» ]
    C/F 626-750 [» ]
    2ZC3 X-ray 2.50 A/D 71-238 [» ]
    B/E 241-625 [» ]
    C/F 626-750 [» ]
    2ZC4 X-ray 2.80 A/D 71-238 [» ]
    B/E 241-625 [» ]
    C/F 626-750 [» ]
    ProteinModelPortali P14677.
    SMRi P14677. Positions 64-750.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 170187.SP_0336.

    Chemistry

    BindingDBi P14677.
    ChEMBLi CHEMBL6188.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK74511 ; AAK74511 ; SP_0336 .
    GeneIDi 930153.
    KEGGi spn:SP_0336.
    PATRICi 19705009. VBIStrPne105772_0350.

    Phylogenomic databases

    eggNOGi COG0768.
    HOGENOMi HOG000049551.
    KOi K12556.
    OMAi KMMKPYV.
    OrthoDBi EOG6N0HHV.

    Enzyme and pathway databases

    BioCyci SPNE170187:GHGN-342-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P14677.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR005543. PASTA_dom.
    IPR005311. PBP_dimer.
    IPR001460. PCN-bd_Tpept.
    [Graphical view ]
    Pfami PF03793. PASTA. 1 hit.
    PF03717. PBP_dimer. 1 hit.
    PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SMARTi SM00740. PASTA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56519. SSF56519. 1 hit.
    SSF56601. SSF56601. 1 hit.
    PROSITEi PS51178. PASTA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-334 / TIGR4.
    2. "X-ray structure of Streptococcus pneumoniae PBP2x, a primary penicillin target enzyme."
      Pares S., Mouz N., Petillot Y., Hakenbeck R., Dideberg O.
      Nat. Struct. Biol. 3:284-289(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 76-750.

    Entry informationi

    Entry nameiPBPX_STRPN
    AccessioniPrimary (citable) accession number: P14677
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The mature form of PBP2x contains an unprocessed signal sequence followed by a membrane-anchoring segment.

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3