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P14677

- PBPX_STRPN

UniProt

P14677 - PBPX_STRPN

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Protein

Penicillin-binding protein 2x

Gene
pbpX, SP_0336
Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei337 – 3371Acyl-ester intermediate By similarity

GO - Molecular functioni

  1. penicillin binding Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. peptidoglycan biosynthetic process Source: UniProtKB-KW
  4. regulation of cell shape Source: UniProtKB-KW
  5. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-342-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 2x
Short name:
PBP-2x
Short name:
PBP2x
Gene namesi
Name:pbpX
Ordered Locus Names:SP_0336
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000585: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4921Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Penicillin-binding protein 2xPRO_0000195453Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi170187.SP_0336.

Structurei

Secondary structure

1
750
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi65 – 728
Beta strandi86 – 916
Beta strandi93 – 997
Beta strandi107 – 1093
Turni116 – 1183
Helixi119 – 13012
Helixi134 – 1418
Beta strandi147 – 1504
Helixi153 – 1553
Beta strandi156 – 1583
Helixi160 – 17011
Turni171 – 1744
Beta strandi177 – 1837
Beta strandi185 – 1873
Beta strandi191 – 1944
Helixi195 – 1984
Beta strandi201 – 2055
Turni207 – 2093
Beta strandi211 – 2155
Helixi218 – 2225
Helixi224 – 2285
Beta strandi232 – 2398
Beta strandi250 – 2534
Beta strandi260 – 2645
Helixi267 – 28418
Beta strandi287 – 2959
Turni296 – 2983
Beta strandi300 – 3089
Turni312 – 3143
Turni327 – 3293
Helixi336 – 3394
Helixi340 – 34910
Beta strandi358 – 3603
Beta strandi364 – 3663
Beta strandi369 – 3713
Helixi374 – 3774
Beta strandi378 – 3803
Beta strandi385 – 3873
Helixi388 – 3936
Helixi397 – 40711
Helixi409 – 41810
Turni419 – 4224
Beta strandi428 – 4314
Helixi442 – 4476
Helixi448 – 4503
Helixi458 – 46912
Turni470 – 4723
Beta strandi480 – 4856
Turni486 – 4894
Beta strandi490 – 4934
Beta strandi498 – 5003
Helixi505 – 52016
Turni522 – 5243
Turni530 – 5323
Beta strandi533 – 5375
Beta strandi545 – 5528
Helixi556 – 5583
Beta strandi559 – 5613
Beta strandi568 – 58013
Beta strandi582 – 59110
Helixi597 – 61721
Helixi625 – 6284
Helixi646 – 6549
Turni655 – 6573
Beta strandi659 – 6657
Beta strandi667 – 6748
Beta strandi685 – 6917
Helixi704 – 71411
Beta strandi717 – 73216
Turni738 – 7403
Beta strandi743 – 7497

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PMDX-ray3.50A76-750[»]
1QMEX-ray2.40A49-750[»]
1QMFX-ray2.80A49-750[»]
1RP5X-ray3.00A/B49-750[»]
2Z2LX-ray2.85A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2Z2MX-ray2.60A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2ZC3X-ray2.50A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2ZC4X-ray2.80A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
ProteinModelPortaliP14677.
SMRiP14677. Positions 64-750.

Miscellaneous databases

EvolutionaryTraceiP14677.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini632 – 69160PASTA 1Add
BLAST
Domaini692 – 75059PASTA 2Add
BLAST

Sequence similaritiesi

Belongs to the transpeptidase family.
Contains 2 PASTA domains.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0768.
HOGENOMiHOG000049551.
KOiK12556.
OMAiKMMKPYV.
OrthoDBiEOG6N0HHV.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR005543. PASTA_dom.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF03793. PASTA. 1 hit.
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 1 hit.
[Graphical view]
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
PROSITEiPS51178. PASTA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14677-1 [UniParc]FASTAAdd to Basket

« Hide

MKWTKRVIRY ATKNRKSPAE NRRRVGKSLS LLSVFVFAIF LVNFAVIIGT    50
GTRFGTDLAK EAKKVHQTTR TVPAKRGTIY DRNGVPIAED ATSYNVYAVI 100
DENYKSATGK ILYVEKTQFN KVAEVFHKYL DMEESYVREQ LSQPNLKQVS 150
FGAKGNGITY ANMMSIKKEL EAAEVKGIDF TTSPNRSYPN GQFASSFIGL 200
AQLHENEDGS KSLLGTSGME SSLNSILAGT DGIITYEKDR LGNIVPGTEQ 250
VSQRTMDGKD VYTTISSPLQ SFMETQMDAF QEKVKGKYMT ATLVSAKTGE 300
ILATTQRPTF DADTKEGITE DFVWRDILYQ SNYEPGSTMK VMMLAAAIDN 350
NTFPGGEVFN SSELKIADAT IRDWDVNEGL TGGRTMTFSQ GFAHSSNVGM 400
TLLEQKMGDA TWLDYLNRFK FGVPTRFGLT DEYAGQLPAD NIVNIAQSSF 450
GQGISVTQTQ MIRAFTAIAN DGVMLEPKFI SAIYDPNDQT ARKSQKEIVG 500
NPVSKDAASL TRTNMVLVGT DPVYGTMYNH STGKPTVTVP GQNVALKSGT 550
AQIADEKNGG YLVGLTDYIF SAVSMSPAEN PDFILYVTVQ QPEHYSGIQL 600
GEFANPILER ASAMKDSLNL QTTAKALEQV SQQSPYPMPS VKDISPGDLA 650
EELRRNLVQP IVVGTGTKIK NSSAEEGKNL APNQQVLILS DKAEEVPDMY 700
GWTKETAETL AKWLNIELEF QGSGSTVQKQ DVRANTAIKD IKKITLTLGD 750
Length:750
Mass (Da):82,313
Last modified:September 26, 2001 - v2
Checksum:i9B2BCADBE4E462E4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005672 Genomic DNA. Translation: AAK74511.1.
PIRiF95039.
H97909.
S06726.
RefSeqiNP_344871.1. NC_003028.3.

Genome annotation databases

EnsemblBacteriaiAAK74511; AAK74511; SP_0336.
GeneIDi930153.
KEGGispn:SP_0336.
PATRICi19705009. VBIStrPne105772_0350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005672 Genomic DNA. Translation: AAK74511.1 .
PIRi F95039.
H97909.
S06726.
RefSeqi NP_344871.1. NC_003028.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PMD X-ray 3.50 A 76-750 [» ]
1QME X-ray 2.40 A 49-750 [» ]
1QMF X-ray 2.80 A 49-750 [» ]
1RP5 X-ray 3.00 A/B 49-750 [» ]
2Z2L X-ray 2.85 A/D 71-238 [» ]
B/E 241-625 [» ]
C/F 626-750 [» ]
2Z2M X-ray 2.60 A/D 71-238 [» ]
B/E 241-625 [» ]
C/F 626-750 [» ]
2ZC3 X-ray 2.50 A/D 71-238 [» ]
B/E 241-625 [» ]
C/F 626-750 [» ]
2ZC4 X-ray 2.80 A/D 71-238 [» ]
B/E 241-625 [» ]
C/F 626-750 [» ]
ProteinModelPortali P14677.
SMRi P14677. Positions 64-750.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 170187.SP_0336.

Chemistry

BindingDBi P14677.
ChEMBLi CHEMBL6188.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK74511 ; AAK74511 ; SP_0336 .
GeneIDi 930153.
KEGGi spn:SP_0336.
PATRICi 19705009. VBIStrPne105772_0350.

Phylogenomic databases

eggNOGi COG0768.
HOGENOMi HOG000049551.
KOi K12556.
OMAi KMMKPYV.
OrthoDBi EOG6N0HHV.

Enzyme and pathway databases

BioCyci SPNE170187:GHGN-342-MONOMER.

Miscellaneous databases

EvolutionaryTracei P14677.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR005543. PASTA_dom.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF03793. PASTA. 1 hit.
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SMARTi SM00740. PASTA. 1 hit.
[Graphical view ]
SUPFAMi SSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
PROSITEi PS51178. PASTA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.
  2. "X-ray structure of Streptococcus pneumoniae PBP2x, a primary penicillin target enzyme."
    Pares S., Mouz N., Petillot Y., Hakenbeck R., Dideberg O.
    Nat. Struct. Biol. 3:284-289(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 76-750.

Entry informationi

Entry nameiPBPX_STRPN
AccessioniPrimary (citable) accession number: P14677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: September 26, 2001
Last modified: September 3, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The mature form of PBP2x contains an unprocessed signal sequence followed by a membrane-anchoring segment.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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