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P14677

- PBPX_STRPN

UniProt

P14677 - PBPX_STRPN

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Protein

Penicillin-binding protein 2x

Gene

pbpX

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei337 – 3371Acyl-ester intermediateBy similarity

GO - Molecular functioni

  1. penicillin binding Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cell division Source: UniProtKB-KW
  3. cell wall organization Source: UniProtKB-KW
  4. peptidoglycan biosynthetic process Source: UniProtKB-KW
  5. regulation of cell shape Source: UniProtKB-KW
  6. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciSPNE170187:GHGN-342-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 2x
Short name:
PBP-2x
Short name:
PBP2x
Gene namesi
Name:pbpX
Ordered Locus Names:SP_0336
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000585: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei29 – 4921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 750750Penicillin-binding protein 2xPRO_0000195453Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi170187.SP_0336.

Structurei

Secondary structure

1
750
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi65 – 728Combined sources
Beta strandi86 – 916Combined sources
Beta strandi93 – 997Combined sources
Beta strandi107 – 1093Combined sources
Turni116 – 1183Combined sources
Helixi119 – 13012Combined sources
Helixi134 – 1418Combined sources
Beta strandi147 – 1504Combined sources
Helixi153 – 1553Combined sources
Beta strandi156 – 1583Combined sources
Helixi160 – 17011Combined sources
Turni171 – 1744Combined sources
Beta strandi177 – 1837Combined sources
Beta strandi185 – 1873Combined sources
Beta strandi191 – 1944Combined sources
Helixi195 – 1984Combined sources
Beta strandi201 – 2055Combined sources
Turni207 – 2093Combined sources
Beta strandi211 – 2155Combined sources
Helixi218 – 2225Combined sources
Helixi224 – 2285Combined sources
Beta strandi232 – 2398Combined sources
Beta strandi250 – 2534Combined sources
Beta strandi260 – 2645Combined sources
Helixi267 – 28418Combined sources
Beta strandi287 – 2959Combined sources
Turni296 – 2983Combined sources
Beta strandi300 – 3089Combined sources
Turni312 – 3143Combined sources
Turni327 – 3293Combined sources
Helixi336 – 3394Combined sources
Helixi340 – 34910Combined sources
Beta strandi358 – 3603Combined sources
Beta strandi364 – 3663Combined sources
Beta strandi369 – 3713Combined sources
Helixi374 – 3774Combined sources
Beta strandi378 – 3803Combined sources
Beta strandi385 – 3873Combined sources
Helixi388 – 3936Combined sources
Helixi397 – 40711Combined sources
Helixi409 – 41810Combined sources
Turni419 – 4224Combined sources
Beta strandi428 – 4314Combined sources
Helixi442 – 4476Combined sources
Helixi448 – 4503Combined sources
Helixi458 – 46912Combined sources
Turni470 – 4723Combined sources
Beta strandi480 – 4856Combined sources
Turni486 – 4894Combined sources
Beta strandi490 – 4934Combined sources
Beta strandi498 – 5003Combined sources
Helixi505 – 52016Combined sources
Turni522 – 5243Combined sources
Turni530 – 5323Combined sources
Beta strandi533 – 5375Combined sources
Beta strandi545 – 5528Combined sources
Helixi556 – 5583Combined sources
Beta strandi559 – 5613Combined sources
Beta strandi568 – 58013Combined sources
Beta strandi582 – 59110Combined sources
Helixi597 – 61721Combined sources
Helixi625 – 6284Combined sources
Helixi646 – 6549Combined sources
Turni655 – 6573Combined sources
Beta strandi659 – 6657Combined sources
Beta strandi667 – 6748Combined sources
Beta strandi685 – 6917Combined sources
Helixi704 – 71411Combined sources
Beta strandi717 – 73216Combined sources
Turni738 – 7403Combined sources
Beta strandi743 – 7497Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PMDX-ray3.50A76-750[»]
1QMEX-ray2.40A49-750[»]
1QMFX-ray2.80A49-750[»]
1RP5X-ray3.00A/B49-750[»]
2Z2LX-ray2.85A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2Z2MX-ray2.60A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2ZC3X-ray2.50A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2ZC4X-ray2.80A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
ProteinModelPortaliP14677.
SMRiP14677. Positions 64-750.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14677.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini632 – 69160PASTA 1PROSITE-ProRule annotationAdd
BLAST
Domaini692 – 75059PASTA 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the transpeptidase family.Curated
Contains 2 PASTA domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0768.
HOGENOMiHOG000049551.
KOiK12556.
OMAiKMMKPYV.
OrthoDBiEOG6N0HHV.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR005543. PASTA_dom.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF03793. PASTA. 1 hit.
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 1 hit.
[Graphical view]
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
PROSITEiPS51178. PASTA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14677-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKWTKRVIRY ATKNRKSPAE NRRRVGKSLS LLSVFVFAIF LVNFAVIIGT
60 70 80 90 100
GTRFGTDLAK EAKKVHQTTR TVPAKRGTIY DRNGVPIAED ATSYNVYAVI
110 120 130 140 150
DENYKSATGK ILYVEKTQFN KVAEVFHKYL DMEESYVREQ LSQPNLKQVS
160 170 180 190 200
FGAKGNGITY ANMMSIKKEL EAAEVKGIDF TTSPNRSYPN GQFASSFIGL
210 220 230 240 250
AQLHENEDGS KSLLGTSGME SSLNSILAGT DGIITYEKDR LGNIVPGTEQ
260 270 280 290 300
VSQRTMDGKD VYTTISSPLQ SFMETQMDAF QEKVKGKYMT ATLVSAKTGE
310 320 330 340 350
ILATTQRPTF DADTKEGITE DFVWRDILYQ SNYEPGSTMK VMMLAAAIDN
360 370 380 390 400
NTFPGGEVFN SSELKIADAT IRDWDVNEGL TGGRTMTFSQ GFAHSSNVGM
410 420 430 440 450
TLLEQKMGDA TWLDYLNRFK FGVPTRFGLT DEYAGQLPAD NIVNIAQSSF
460 470 480 490 500
GQGISVTQTQ MIRAFTAIAN DGVMLEPKFI SAIYDPNDQT ARKSQKEIVG
510 520 530 540 550
NPVSKDAASL TRTNMVLVGT DPVYGTMYNH STGKPTVTVP GQNVALKSGT
560 570 580 590 600
AQIADEKNGG YLVGLTDYIF SAVSMSPAEN PDFILYVTVQ QPEHYSGIQL
610 620 630 640 650
GEFANPILER ASAMKDSLNL QTTAKALEQV SQQSPYPMPS VKDISPGDLA
660 670 680 690 700
EELRRNLVQP IVVGTGTKIK NSSAEEGKNL APNQQVLILS DKAEEVPDMY
710 720 730 740 750
GWTKETAETL AKWLNIELEF QGSGSTVQKQ DVRANTAIKD IKKITLTLGD
Length:750
Mass (Da):82,313
Last modified:September 26, 2001 - v2
Checksum:i9B2BCADBE4E462E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74511.1.
PIRiF95039.
H97909.
S06726.
RefSeqiNP_344871.1. NC_003028.3.

Genome annotation databases

EnsemblBacteriaiAAK74511; AAK74511; SP_0336.
GeneIDi930153.
KEGGispn:SP_0336.
PATRICi19705009. VBIStrPne105772_0350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74511.1 .
PIRi F95039.
H97909.
S06726.
RefSeqi NP_344871.1. NC_003028.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PMD X-ray 3.50 A 76-750 [» ]
1QME X-ray 2.40 A 49-750 [» ]
1QMF X-ray 2.80 A 49-750 [» ]
1RP5 X-ray 3.00 A/B 49-750 [» ]
2Z2L X-ray 2.85 A/D 71-238 [» ]
B/E 241-625 [» ]
C/F 626-750 [» ]
2Z2M X-ray 2.60 A/D 71-238 [» ]
B/E 241-625 [» ]
C/F 626-750 [» ]
2ZC3 X-ray 2.50 A/D 71-238 [» ]
B/E 241-625 [» ]
C/F 626-750 [» ]
2ZC4 X-ray 2.80 A/D 71-238 [» ]
B/E 241-625 [» ]
C/F 626-750 [» ]
ProteinModelPortali P14677.
SMRi P14677. Positions 64-750.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 170187.SP_0336.

Chemistry

BindingDBi P14677.
ChEMBLi CHEMBL6188.
DrugBanki DB01150. Cefprozil.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK74511 ; AAK74511 ; SP_0336 .
GeneIDi 930153.
KEGGi spn:SP_0336.
PATRICi 19705009. VBIStrPne105772_0350.

Phylogenomic databases

eggNOGi COG0768.
HOGENOMi HOG000049551.
KOi K12556.
OMAi KMMKPYV.
OrthoDBi EOG6N0HHV.

Enzyme and pathway databases

BioCyci SPNE170187:GHGN-342-MONOMER.

Miscellaneous databases

EvolutionaryTracei P14677.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR005543. PASTA_dom.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF03793. PASTA. 1 hit.
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SMARTi SM00740. PASTA. 1 hit.
[Graphical view ]
SUPFAMi SSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
PROSITEi PS51178. PASTA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-334 / TIGR4.
  2. "X-ray structure of Streptococcus pneumoniae PBP2x, a primary penicillin target enzyme."
    Pares S., Mouz N., Petillot Y., Hakenbeck R., Dideberg O.
    Nat. Struct. Biol. 3:284-289(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 76-750.

Entry informationi

Entry nameiPBPX_STRPN
AccessioniPrimary (citable) accession number: P14677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: September 26, 2001
Last modified: November 26, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The mature form of PBP2x contains an unprocessed signal sequence followed by a membrane-anchoring segment.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3