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Protein

Penicillin-binding protein 2x

Gene

pbpX

Organism
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. Beta-lactams inactivate the PBPs by acylating an essential serine residue in the active site of these proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei337Acyl-ester intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BRENDAi2.4.1.129. 1960.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 2x
Short name:
PBP-2x
Short name:
PBP2x
Gene namesi
Name:pbpX
Ordered Locus Names:SP_0336
OrganismiStreptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Taxonomic identifieri170187 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000000585 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL6188.
DrugBankiDB01150. Cefprozil.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954531 – 750Penicillin-binding protein 2xAdd BLAST750

Interactioni

Protein-protein interaction databases

STRINGi170187.SpneT_02001589.

Chemistry databases

BindingDBiP14677.

Structurei

Secondary structure

1750
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi65 – 72Combined sources8
Beta strandi86 – 91Combined sources6
Beta strandi93 – 99Combined sources7
Beta strandi107 – 109Combined sources3
Turni116 – 118Combined sources3
Helixi119 – 130Combined sources12
Helixi134 – 141Combined sources8
Beta strandi147 – 150Combined sources4
Helixi153 – 155Combined sources3
Beta strandi156 – 158Combined sources3
Helixi160 – 170Combined sources11
Turni171 – 174Combined sources4
Beta strandi177 – 183Combined sources7
Beta strandi185 – 187Combined sources3
Beta strandi191 – 194Combined sources4
Helixi195 – 198Combined sources4
Beta strandi201 – 205Combined sources5
Turni207 – 209Combined sources3
Beta strandi211 – 215Combined sources5
Helixi218 – 222Combined sources5
Helixi224 – 228Combined sources5
Beta strandi232 – 239Combined sources8
Beta strandi250 – 253Combined sources4
Beta strandi260 – 264Combined sources5
Helixi267 – 284Combined sources18
Beta strandi287 – 295Combined sources9
Turni296 – 298Combined sources3
Beta strandi300 – 308Combined sources9
Turni312 – 314Combined sources3
Turni327 – 329Combined sources3
Helixi336 – 339Combined sources4
Helixi340 – 349Combined sources10
Beta strandi358 – 360Combined sources3
Beta strandi364 – 366Combined sources3
Beta strandi369 – 371Combined sources3
Helixi374 – 377Combined sources4
Beta strandi378 – 380Combined sources3
Beta strandi385 – 387Combined sources3
Helixi388 – 393Combined sources6
Helixi397 – 407Combined sources11
Helixi409 – 418Combined sources10
Turni419 – 422Combined sources4
Beta strandi428 – 431Combined sources4
Helixi442 – 447Combined sources6
Helixi448 – 450Combined sources3
Helixi458 – 469Combined sources12
Turni470 – 472Combined sources3
Beta strandi480 – 485Combined sources6
Turni486 – 489Combined sources4
Beta strandi490 – 493Combined sources4
Beta strandi498 – 500Combined sources3
Helixi505 – 520Combined sources16
Turni522 – 524Combined sources3
Turni530 – 532Combined sources3
Beta strandi533 – 537Combined sources5
Beta strandi545 – 552Combined sources8
Helixi556 – 558Combined sources3
Beta strandi559 – 561Combined sources3
Beta strandi568 – 580Combined sources13
Beta strandi582 – 591Combined sources10
Helixi597 – 617Combined sources21
Helixi625 – 628Combined sources4
Helixi646 – 654Combined sources9
Turni655 – 657Combined sources3
Beta strandi659 – 665Combined sources7
Beta strandi667 – 674Combined sources8
Beta strandi685 – 691Combined sources7
Helixi704 – 714Combined sources11
Beta strandi717 – 732Combined sources16
Turni738 – 740Combined sources3
Beta strandi743 – 749Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PMDX-ray3.50A76-750[»]
1QMEX-ray2.40A49-750[»]
1QMFX-ray2.80A49-750[»]
1RP5X-ray3.00A/B49-750[»]
2Z2LX-ray2.85A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2Z2MX-ray2.60A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2ZC3X-ray2.50A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2ZC4X-ray2.80A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
ProteinModelPortaliP14677.
SMRiP14677.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14677.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini632 – 691PASTA 1PROSITE-ProRule annotationAdd BLAST60
Domaini692 – 750PASTA 2PROSITE-ProRule annotationAdd BLAST59

Sequence similaritiesi

Belongs to the transpeptidase family.Curated
Contains 2 PASTA domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJN. Bacteria.
COG0768. LUCA.
HOGENOMiHOG000049551.
KOiK12556.
OMAiGTVHNQD.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR005543. PASTA_dom.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF03793. PASTA. 1 hit.
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 1 hit.
[Graphical view]
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
PROSITEiPS51178. PASTA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWTKRVIRY ATKNRKSPAE NRRRVGKSLS LLSVFVFAIF LVNFAVIIGT
60 70 80 90 100
GTRFGTDLAK EAKKVHQTTR TVPAKRGTIY DRNGVPIAED ATSYNVYAVI
110 120 130 140 150
DENYKSATGK ILYVEKTQFN KVAEVFHKYL DMEESYVREQ LSQPNLKQVS
160 170 180 190 200
FGAKGNGITY ANMMSIKKEL EAAEVKGIDF TTSPNRSYPN GQFASSFIGL
210 220 230 240 250
AQLHENEDGS KSLLGTSGME SSLNSILAGT DGIITYEKDR LGNIVPGTEQ
260 270 280 290 300
VSQRTMDGKD VYTTISSPLQ SFMETQMDAF QEKVKGKYMT ATLVSAKTGE
310 320 330 340 350
ILATTQRPTF DADTKEGITE DFVWRDILYQ SNYEPGSTMK VMMLAAAIDN
360 370 380 390 400
NTFPGGEVFN SSELKIADAT IRDWDVNEGL TGGRTMTFSQ GFAHSSNVGM
410 420 430 440 450
TLLEQKMGDA TWLDYLNRFK FGVPTRFGLT DEYAGQLPAD NIVNIAQSSF
460 470 480 490 500
GQGISVTQTQ MIRAFTAIAN DGVMLEPKFI SAIYDPNDQT ARKSQKEIVG
510 520 530 540 550
NPVSKDAASL TRTNMVLVGT DPVYGTMYNH STGKPTVTVP GQNVALKSGT
560 570 580 590 600
AQIADEKNGG YLVGLTDYIF SAVSMSPAEN PDFILYVTVQ QPEHYSGIQL
610 620 630 640 650
GEFANPILER ASAMKDSLNL QTTAKALEQV SQQSPYPMPS VKDISPGDLA
660 670 680 690 700
EELRRNLVQP IVVGTGTKIK NSSAEEGKNL APNQQVLILS DKAEEVPDMY
710 720 730 740 750
GWTKETAETL AKWLNIELEF QGSGSTVQKQ DVRANTAIKD IKKITLTLGD
Length:750
Mass (Da):82,313
Last modified:September 26, 2001 - v2
Checksum:i9B2BCADBE4E462E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74511.1.
PIRiF95039.
H97909.
S06726.
RefSeqiWP_000872275.1. NZ_AKVY01000001.1.

Genome annotation databases

EnsemblBacteriaiAAK74511; AAK74511; SP_0336.
KEGGispn:SP_0336.
PATRICi19705009. VBIStrPne105772_0350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005672 Genomic DNA. Translation: AAK74511.1.
PIRiF95039.
H97909.
S06726.
RefSeqiWP_000872275.1. NZ_AKVY01000001.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PMDX-ray3.50A76-750[»]
1QMEX-ray2.40A49-750[»]
1QMFX-ray2.80A49-750[»]
1RP5X-ray3.00A/B49-750[»]
2Z2LX-ray2.85A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2Z2MX-ray2.60A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2ZC3X-ray2.50A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
2ZC4X-ray2.80A/D71-238[»]
B/E241-625[»]
C/F626-750[»]
ProteinModelPortaliP14677.
SMRiP14677.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi170187.SpneT_02001589.

Chemistry databases

BindingDBiP14677.
ChEMBLiCHEMBL6188.
DrugBankiDB01150. Cefprozil.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK74511; AAK74511; SP_0336.
KEGGispn:SP_0336.
PATRICi19705009. VBIStrPne105772_0350.

Phylogenomic databases

eggNOGiENOG4105CJN. Bacteria.
COG0768. LUCA.
HOGENOMiHOG000049551.
KOiK12556.
OMAiGTVHNQD.

Enzyme and pathway databases

BRENDAi2.4.1.129. 1960.

Miscellaneous databases

EvolutionaryTraceiP14677.
PROiP14677.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR005543. PASTA_dom.
IPR005311. PBP_dimer.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF03793. PASTA. 1 hit.
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 1 hit.
[Graphical view]
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
PROSITEiPS51178. PASTA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPBPX_STRPN
AccessioniPrimary (citable) accession number: P14677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: September 26, 2001
Last modified: November 2, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The mature form of PBP2x contains an unprocessed signal sequence followed by a membrane-anchoring segment.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.