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Protein

Tryptophan synthase beta chain 1, chloroplastic

Gene

TSB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase beta chain 2, chloroplastic (TSB2)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • pyridoxal phosphate binding Source: GO_Central
  • tryptophan synthase activity Source: TAIR

GO - Biological processi

  • indoleacetic acid biosynthetic process Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT5G54810-MONOMER.
MetaCyc:AT5G54810-MONOMER.
BRENDAi4.2.1.20. 399.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain 1, chloroplastic (EC:4.2.1.20)
Gene namesi
Name:TSB1
Ordered Locus Names:At5g54810
ORF Names:MBG8.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G54810.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 470Tryptophan synthase beta chain 1, chloroplasticPRO_0000035783
Transit peptidei1 – ?ChloroplastSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei165 – 1651N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiP14671.
PRIDEiP14671.

Expressioni

Gene expression databases

ExpressionAtlasiP14671. baseline and differential.
GenevisibleiP14671. AT.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.

Protein-protein interaction databases

BioGridi20815. 2 interactions.
IntActiP14671. 1 interaction.
STRINGi3702.AT5G54810.1.

Structurei

3D structure databases

ProteinModelPortaliP14671.
SMRiP14671. Positions 81-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1395. Eukaryota.
COG0133. LUCA.
HOGENOMiHOG000161710.
InParanoidiP14671.
KOiK01696.
OMAiTTVERTH.
OrthoDBiEOG093609DQ.
PhylomeDBiP14671.

Family and domain databases

HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASGTSATF RASVSSAPSS SSQLTHLKSP FKAVKYTPLP SSRSKSSSFS
60 70 80 90 100
VSCTIAKDPP VLMAAGSDPA LWQRPDSFGR FGKFGGKYVP ETLMHALSEL
110 120 130 140 150
ESAFYALATD DDFQRELAGI LKDYVGRESP LYFAERLTEH YRRENGEGPL
160 170 180 190 200
IYLKREDLNH TGAHKINNAV AQALLAKRLG KKRIIAETGA GQHGVATATV
210 220 230 240 250
CARFGLECII YMGAQDMERQ ALNVFRMRLL GAEVRGVHSG TATLKDATSE
260 270 280 290 300
AIRDWVTNVE TTHYILGSVA GPHPYPMMVR DFHAVIGKET RKQALEKWGG
310 320 330 340 350
KPDVLVACVG GGSNAMGLFH EFVNDTEVRM IGVEAAGFGL DSGKHAATLT
360 370 380 390 400
KGDVGVLHGA MSYLLQDDDG QIIEPHSISA GLDYPGVGPE HSFFKDMGRA
410 420 430 440 450
EYYSITDEEA LEAFKRVSRL EGIIPALETS HALAYLEKLC PTLSDGTRVV
460 470
LNFSGRGDKD VQTVAKYLDV
Length:470
Mass (Da):50,926
Last modified:April 1, 1990 - v1
Checksum:i2EA63F685927FEE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23872 Genomic DNA. Translation: AAA32878.1.
AB005232 Genomic DNA. Translation: BAB08760.1.
CP002688 Genomic DNA. Translation: AED96543.1.
AF367264 mRNA. Translation: AAK56253.1.
AY133620 mRNA. Translation: AAM91450.1.
AY087382 mRNA. Translation: AAM64932.1.
PIRiA33929. A31393.
RefSeqiNP_200292.1. NM_124862.4.
UniGeneiAt.23506.

Genome annotation databases

EnsemblPlantsiAT5G54810.1; AT5G54810.1; AT5G54810.
GeneIDi835571.
GrameneiAT5G54810.1; AT5G54810.1; AT5G54810.
KEGGiath:AT5G54810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23872 Genomic DNA. Translation: AAA32878.1.
AB005232 Genomic DNA. Translation: BAB08760.1.
CP002688 Genomic DNA. Translation: AED96543.1.
AF367264 mRNA. Translation: AAK56253.1.
AY133620 mRNA. Translation: AAM91450.1.
AY087382 mRNA. Translation: AAM64932.1.
PIRiA33929. A31393.
RefSeqiNP_200292.1. NM_124862.4.
UniGeneiAt.23506.

3D structure databases

ProteinModelPortaliP14671.
SMRiP14671. Positions 81-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20815. 2 interactions.
IntActiP14671. 1 interaction.
STRINGi3702.AT5G54810.1.

Proteomic databases

PaxDbiP14671.
PRIDEiP14671.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G54810.1; AT5G54810.1; AT5G54810.
GeneIDi835571.
GrameneiAT5G54810.1; AT5G54810.1; AT5G54810.
KEGGiath:AT5G54810.

Organism-specific databases

TAIRiAT5G54810.

Phylogenomic databases

eggNOGiKOG1395. Eukaryota.
COG0133. LUCA.
HOGENOMiHOG000161710.
InParanoidiP14671.
KOiK01696.
OMAiTTVERTH.
OrthoDBiEOG093609DQ.
PhylomeDBiP14671.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciARA:AT5G54810-MONOMER.
MetaCyc:AT5G54810-MONOMER.
BRENDAi4.2.1.20. 399.

Miscellaneous databases

PROiP14671.

Gene expression databases

ExpressionAtlasiP14671. baseline and differential.
GenevisibleiP14671. AT.

Family and domain databases

HAMAPiMF_00133. Trp_synth_beta. 1 hit.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPB1_ARATH
AccessioniPrimary (citable) accession number: P14671
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.