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Protein

Tryptophan synthase beta chain 1, chloroplastic

Gene

TSB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (AXX17_At5g53930), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (AXX17_At5g28490), Tryptophan synthase (AXX17_At4g31220), Tryptophan synthase, Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 2, chloroplastic (TSB2), Tryptophan synthase (AXX17_At4g31220)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • protein domain specific binding Source: CAFA
  • tryptophan synthase activity Source: TAIR

GO - Biological processi

  • indoleacetic acid biosynthetic process Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to salt stress Source: TAIR

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT5G54810-MONOMER
MetaCyc:AT5G54810-MONOMER
BRENDAi4.2.1.20 399
UniPathwayiUPA00035; UER00044

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain 1, chloroplastic (EC:4.2.1.20)
Gene namesi
Name:TSB1
Ordered Locus Names:At5g54810
ORF Names:MBG8.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G54810
TAIRilocus:2160190 AT5G54810

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000035783? – 470Tryptophan synthase beta chain 1, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei165N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiP14671
PRIDEiP14671

Expressioni

Gene expression databases

ExpressionAtlasiP14671 baseline and differential
GenevisibleiP14671 AT

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi20815, 2 interactors
IntActiP14671, 1 interactor
STRINGi3702.AT5G54810.1

Structurei

3D structure databases

ProteinModelPortaliP14671
SMRiP14671
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1395 Eukaryota
COG0133 LUCA
HOGENOMiHOG000161710
InParanoidiP14671
KOiK01696
OMAiHGMKSYF
OrthoDBiEOG093609DQ
PhylomeDBiP14671

Family and domain databases

CDDicd06446 Trp-synth_B, 1 hit
HAMAPiMF_00133 Trp_synth_beta, 1 hit
InterProiView protein in InterPro
IPR001926 PLP-dep
IPR006653 Trp_synth_b_CS
IPR006654 Trp_synth_beta
IPR023026 Trp_synth_beta/beta-like
IPR036052 Trypto_synt_PLP_dependent
PANTHERiPTHR42882 PTHR42882, 1 hit
PfamiView protein in Pfam
PF00291 PALP, 1 hit
PIRSFiPIRSF001413 Trp_syn_beta, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR00263 trpB, 1 hit
PROSITEiView protein in PROSITE
PS00168 TRP_SYNTHASE_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASGTSATF RASVSSAPSS SSQLTHLKSP FKAVKYTPLP SSRSKSSSFS
60 70 80 90 100
VSCTIAKDPP VLMAAGSDPA LWQRPDSFGR FGKFGGKYVP ETLMHALSEL
110 120 130 140 150
ESAFYALATD DDFQRELAGI LKDYVGRESP LYFAERLTEH YRRENGEGPL
160 170 180 190 200
IYLKREDLNH TGAHKINNAV AQALLAKRLG KKRIIAETGA GQHGVATATV
210 220 230 240 250
CARFGLECII YMGAQDMERQ ALNVFRMRLL GAEVRGVHSG TATLKDATSE
260 270 280 290 300
AIRDWVTNVE TTHYILGSVA GPHPYPMMVR DFHAVIGKET RKQALEKWGG
310 320 330 340 350
KPDVLVACVG GGSNAMGLFH EFVNDTEVRM IGVEAAGFGL DSGKHAATLT
360 370 380 390 400
KGDVGVLHGA MSYLLQDDDG QIIEPHSISA GLDYPGVGPE HSFFKDMGRA
410 420 430 440 450
EYYSITDEEA LEAFKRVSRL EGIIPALETS HALAYLEKLC PTLSDGTRVV
460 470
LNFSGRGDKD VQTVAKYLDV
Length:470
Mass (Da):50,926
Last modified:April 1, 1990 - v1
Checksum:i2EA63F685927FEE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23872 Genomic DNA Translation: AAA32878.1
AB005232 Genomic DNA Translation: BAB08760.1
CP002688 Genomic DNA Translation: AED96543.1
AF367264 mRNA Translation: AAK56253.1
AY133620 mRNA Translation: AAM91450.1
AY087382 mRNA Translation: AAM64932.1
PIRiA33929 A31393
RefSeqiNP_200292.1, NM_124862.5
UniGeneiAt.23506

Genome annotation databases

EnsemblPlantsiAT5G54810.1; AT5G54810.1; AT5G54810
GeneIDi835571
GrameneiAT5G54810.1; AT5G54810.1; AT5G54810
KEGGiath:AT5G54810

Similar proteinsi

Entry informationi

Entry nameiTRPB1_ARATH
AccessioniPrimary (citable) accession number: P14671
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 25, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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