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P14657 (DHE4_UNKP) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
Gene names
Name:gdhA
OrganismUnknown prokaryotic organism
Taxonomic identifier2725 [NCBI]
Taxonomic lineageBacteriaenvironmental samples

Protein attributes

Sequence length446 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidative deamination of glutamate to a-ketoglutarate and ammonia By similarity.

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Caution

This sequence originates from an organism contaminating a Chlorella sorokiniana culture, probably a bacterium.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processglutamate biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 446446NADP-specific glutamate dehydrogenase
PRO_0000182778

Sites

Active site1281Proton donor By similarity
Binding site921Substrate By similarity
Binding site1131Substrate By similarity
Binding site1161Substrate By similarity
Binding site1671Substrate; via carbonyl oxygen By similarity
Binding site2111NADP By similarity
Binding site2421NADP By similarity
Binding site3791Substrate By similarity
Site1681Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P14657 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: EDCD53EF87F1A090

FASTA44648,490
        10         20         30         40         50         60 
MEYAVSVESF LSSLQRHNPH QPEYLQAVRE VFTSLWPFIE RNPAYREQAL LERLVEPERI 

        70         80         90        100        110        120 
IQFRVSWVDD RGQVQVNRAF RVQFNSAIGP YKGGMRFHPS VNLSILKFLG FEQTFKNALT 

       130        140        150        160        170        180 
TLPMGGGKGG SDFDPKGKSQ GRIMRFCQAL MTELYRHLGP DTDVPAGDIG DGGREVGFMA 

       190        200        210        220        230        240 
GMMKKLSNNT ACVFTGKGLS FGGSLIRPEA TGYGLVYFTD AMLQRHGLGF EGMRVAVSGS 

       250        260        270        280        290        300 
GNVAQYTIEK ALELDARVIT VSDSGGTLVD EDGFTTEKLA HLAEIKNQRY GRVADYAAER 

       310        320        330        340        350        360 
GLTYLAGQQP WNVPVDIALP CATQNELDLE AARVIRNGVK AVAEGANMPT TIQATDAFLD 

       370        380        390        400        410        420 
AGVLFAPGKA ANAAGLATSG LEMAQNAARI GWRAEKVDVR LQHIMADIHH ACVEYGGEGK 

       430        440 
QTHYVHGANI AGFVKVAEAM LAQGVL 

« Hide

References

[1]"A glutamate dehydrogenase gene sequence."
Cock J.M., Schmidt R.R.
Nucleic Acids Res. 17:10500-10500(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16399 Genomic DNA. Translation: CAA34434.1.

3D structure databases

ProteinModelPortalP14657.
SMRP14657. Positions 6-446.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP14657.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_UNKP
AccessionPrimary (citable) accession number: P14657
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 29, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families