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P14656 (GLN11_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase cytosolic isozyme 1-1

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase GLN1;1
Short name=OsGLN1;1
Glutamine synthetase shoot isozyme
OsGS1;1
Gene names
Name:GLN1-1
Synonyms:RGS28
Ordered Locus Names:Os02g0735200, LOC_Os02g50240
ORF Names:P0487D09.8
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High-affinity glutamine synthetase. May be a major component of the cytosolic glutamine synthetic pathway in leaf blades. Ref.7

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Cytoplasm.

Tissue specificity

Highly expressed in leaf blades, at intermediate levels in spikelets (rice flower) and at lower levels in roots. Ref.6 Ref.7

Developmental stage

Expressed in metaxylem and phloem of parenchyma cells and in sclerenchyma cells of developing leaf blades. Ref.5

Induction

By ammonium supply under nitrogen-limited condition. Ref.7

Disruption phenotype

Plants show marked reduction in leaf blades elongation, plant height, panicle size and grain filling. Ref.7

Sequence similarities

Belongs to the glutamine synthetase family.

Biophysicochemical properties

Kinetic parameters:

Measured at pH 7.8 and 30 degrees Celsius for all experiments.

KM=1.9 mM for glutamate Ref.6

KM=27 µM for ammonium

KM=450 µM for ATP

Vmax=190 nmol/sec/mg enzyme with glutamate as substrate

Vmax=186.3 nmol/sec/mg enzyme with ammonium as substrate

Vmax=170.2 nmol/sec/mg enzyme with ATP as substrate

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

leaf senescence

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentcell wall

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytosolic ribosome

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

plasma membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

plasmodesma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

copper ion binding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Glutamine synthetase cytosolic isozyme 1-1
PRO_0000153187

Sequences

Sequence LengthMass (Da)Tools
P14656 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: 7D21F7C31B7CB4DD

FASTA35639,201
        10         20         30         40         50         60 
MASLTDLVNL NLSDTTEKII AEYIWIGGSG MDLRSKARTL SGPVTDPSKL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGEDSEV ILYPQAIFKD PFRKGNNILV MCDCYTPAGE PIPTNKRHNA AKIFSSPEVA 

       130        140        150        160        170        180 
SEEPWYGIEQ EYTLLQKDIN WPLGWPVGGF PGPQGPYYCG IGADKSFGRD IVDSHYKACL 

       190        200        210        220        230        240 
YAGINISGIN GEVMPGQWEF QVGPSVGISA GDQVWVARYI LERITEIAGV VVSFDPKPIP 

       250        260        270        280        290        300 
GDWNGAGAHT NYSTKSMRND GGYEIIKSAI EKLKLRHKEH ISAYGEGNER RLTGRHETAD 

       310        320        330        340        350 
INTFSWGVAN RGASVRVGRE TEQNGKGYFE DRRPASNMDP YIVTSMIAET TIIWKP 

« Hide

References

« Hide 'large scale' references
[1]"Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
Plant Mol. Biol. 13:611-614(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Kinmaze.
Tissue: Shoot.
[2]"Nucleotide sequence of a genomic DNA and a cDNA encoding cytosolic glutamine synthetase in Sasanishiki, a seading cultivar of rice (Oryza sativa L) in northern Japan."
Kojima S., Hanzawa S., Hayakawa T., Hayashi M., Yamaya T.
Plant Gene Register PGR00-048
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Sasanishiki.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 204-213.
Strain: cv. Nipponbare.
Tissue: Callus.
[5]"Overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice."
Sakurai N., Katayama Y., Yamaya T.
Physiol. Plantarum 113:400-408(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[6]"Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots."
Ishiyama K., Inoue E., Tabuchi M., Yamaya T., Takahashi H.
Plant Cell Physiol. 45:1640-1647(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
[7]"Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1."
Tabuchi M., Sugiyama K., Ishiyama K., Inoue E., Sato T., Takahashi H., Yamaya T.
Plant J. 42:641-651(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14245 mRNA. Translation: CAA32461.1.
AB037595 mRNA. Translation: BAA95679.1.
AB037664 Genomic DNA. Translation: BAA95678.1.
AP004880 Genomic DNA. Translation: BAD15892.1.
PIRAJRZQG. S07470.
RefSeqNP_001048045.1. NM_001054580.1.
UniGeneOs.7909.

3D structure databases

ProteinModelPortalP14656.
SMRP14656. Positions 4-355.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP14656. 1 interaction.

Proteomic databases

PaxDbP14656.
PRIDEP14656.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS02T0735200-01; OS02T0735200-01; OS02G0735200.
OS02T0735200-02; OS02T0735200-02; OS02G0735200.
OS02T0735200-03; OS02T0735200-03; OS02G0735200.
GeneID4330649.
KEGGosa:4330649.

Organism-specific databases

GrameneP14656.

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000061500.
KOK01915.
OMAHETSNIF.

Enzyme and pathway databases

BRENDA6.3.1.2. 4460.
SABIO-RKP14656.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLN11_ORYSJ
AccessionPrimary (citable) accession number: P14656
Secondary accession number(s): Q6Z753
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 14, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations