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P14656

- GLN11_ORYSJ

UniProt

P14656 - GLN11_ORYSJ

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Protein

Glutamine synthetase cytosolic isozyme 1-1

Gene

GLN1-1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

High-affinity glutamine synthetase. May be a major component of the cytosolic glutamine synthetic pathway in leaf blades.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=1.9 mM for glutamate1 Publication
  2. KM=27 µM for ammonium1 Publication
  3. KM=450 µM for ATP1 Publication

Vmax=190 nmol/sec/mg enzyme with glutamate as substrate1 Publication

Vmax=186.3 nmol/sec/mg enzyme with ammonium as substrate1 Publication

Vmax=170.2 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 4460.
ReactomeiREACT_243724. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
REACT_250903. Amino acid synthesis and interconversion (transamination).
SABIO-RKP14656.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-1 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;1
Short name:
OsGLN1;1
Glutamine synthetase shoot isozyme
OsGS1;1
Gene namesi
Name:GLN1-1
Synonyms:RGS28
Ordered Locus Names:Os02g0735200, LOC_Os02g50240
ORF Names:P0487D09.8
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 2

Organism-specific databases

GrameneiP14656.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Plants show marked reduction in leaf blades elongation, plant height, panicle size and grain filling.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Glutamine synthetase cytosolic isozyme 1-1PRO_0000153187Add
BLAST

Proteomic databases

PaxDbiP14656.
PRIDEiP14656.

Expressioni

Tissue specificityi

Highly expressed in leaf blades, at intermediate levels in spikelets (rice flower) and at lower levels in roots.2 Publications

Developmental stagei

Expressed in metaxylem and phloem of parenchyma cells and in sclerenchyma cells of developing leaf blades.1 Publication

Inductioni

By ammonium supply under nitrogen-limited condition.1 Publication

Gene expression databases

ExpressionAtlasiP14656. baseline and differential.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

IntActiP14656. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP14656.
SMRiP14656. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiP14656.
KOiK01915.
OMAiHETSNIF.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14656-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASLTDLVNL NLSDTTEKII AEYIWIGGSG MDLRSKARTL SGPVTDPSKL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRKGNNILV MCDCYTPAGE
110 120 130 140 150
PIPTNKRHNA AKIFSSPEVA SEEPWYGIEQ EYTLLQKDIN WPLGWPVGGF
160 170 180 190 200
PGPQGPYYCG IGADKSFGRD IVDSHYKACL YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGISA GDQVWVARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHT
260 270 280 290 300
NYSTKSMRND GGYEIIKSAI EKLKLRHKEH ISAYGEGNER RLTGRHETAD
310 320 330 340 350
INTFSWGVAN RGASVRVGRE TEQNGKGYFE DRRPASNMDP YIVTSMIAET

TIIWKP
Length:356
Mass (Da):39,201
Last modified:April 1, 1990 - v1
Checksum:i7D21F7C31B7CB4DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14245 mRNA. Translation: CAA32461.1.
AB037595 mRNA. Translation: BAA95679.1.
AB037664 Genomic DNA. Translation: BAA95678.1.
AP004880 Genomic DNA. Translation: BAD15892.1.
PIRiS07470. AJRZQG.
RefSeqiNP_001048045.1. NM_001054580.1.
UniGeneiOs.7909.

Genome annotation databases

EnsemblPlantsiOS02T0735200-01; OS02T0735200-01; OS02G0735200.
OS02T0735200-02; OS02T0735200-02; OS02G0735200.
OS02T0735200-03; OS02T0735200-03; OS02G0735200.
GeneIDi4330649.
KEGGiosa:4330649.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14245 mRNA. Translation: CAA32461.1 .
AB037595 mRNA. Translation: BAA95679.1 .
AB037664 Genomic DNA. Translation: BAA95678.1 .
AP004880 Genomic DNA. Translation: BAD15892.1 .
PIRi S07470. AJRZQG.
RefSeqi NP_001048045.1. NM_001054580.1.
UniGenei Os.7909.

3D structure databases

ProteinModelPortali P14656.
SMRi P14656. Positions 4-355.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P14656. 1 interaction.

Proteomic databases

PaxDbi P14656.
PRIDEi P14656.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS02T0735200-01 ; OS02T0735200-01 ; OS02G0735200 .
OS02T0735200-02 ; OS02T0735200-02 ; OS02G0735200 .
OS02T0735200-03 ; OS02T0735200-03 ; OS02G0735200 .
GeneIDi 4330649.
KEGGi osa:4330649.

Organism-specific databases

Gramenei P14656.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000061500.
InParanoidi P14656.
KOi K01915.
OMAi HETSNIF.

Enzyme and pathway databases

BRENDAi 6.3.1.2. 4460.
Reactomei REACT_243724. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
REACT_250903. Amino acid synthesis and interconversion (transamination).
SABIO-RK P14656.

Gene expression databases

ExpressionAtlasi P14656. baseline and differential.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
    Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
    Plant Mol. Biol. 13:611-614(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Kinmaze.
    Tissue: Shoot.
  2. "Nucleotide sequence of a genomic DNA and a cDNA encoding cytosolic glutamine synthetase in Sasanishiki, a seading cultivar of rice (Oryza sativa L) in northern Japan."
    Kojima S., Hanzawa S., Hayakawa T., Hayashi M., Yamaya T.
    Plant Gene Register PGR00-048
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Sasanishiki.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
    Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
    Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 204-213.
    Strain: cv. Nipponbare.
    Tissue: Callus.
  5. "Overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice."
    Sakurai N., Katayama Y., Yamaya T.
    Physiol. Plantarum 113:400-408(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots."
    Ishiyama K., Inoue E., Tabuchi M., Yamaya T., Takahashi H.
    Plant Cell Physiol. 45:1640-1647(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
  7. "Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1."
    Tabuchi M., Sugiyama K., Ishiyama K., Inoue E., Sato T., Takahashi H., Yamaya T.
    Plant J. 42:641-651(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiGLN11_ORYSJ
AccessioniPrimary (citable) accession number: P14656
Secondary accession number(s): Q6Z753
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3