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P14656

- GLN11_ORYSJ

UniProt

P14656 - GLN11_ORYSJ

Protein

Glutamine synthetase cytosolic isozyme 1-1

Gene

GLN1-1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    High-affinity glutamine synthetase. May be a major component of the cytosolic glutamine synthetic pathway in leaf blades.1 Publication

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Kineticsi

    Measured at pH 7.8 and 30 degrees Celsius for all experiments.

    1. KM=1.9 mM for glutamate1 Publication
    2. KM=27 µM for ammonium1 Publication
    3. KM=450 µM for ATP1 Publication

    Vmax=190 nmol/sec/mg enzyme with glutamate as substrate1 Publication

    Vmax=186.3 nmol/sec/mg enzyme with ammonium as substrate1 Publication

    Vmax=170.2 nmol/sec/mg enzyme with ATP as substrate1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. copper ion binding Source: EnsemblPlants/Gramene
    3. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. leaf senescence Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.3.1.2. 4460.
    SABIO-RKP14656.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme 1-1 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase GLN1;1
    Short name:
    OsGLN1;1
    Glutamine synthetase shoot isozyme
    OsGS1;1
    Gene namesi
    Name:GLN1-1
    Synonyms:RGS28
    Ordered Locus Names:Os02g0735200, LOC_Os02g50240
    ORF Names:P0487D09.8
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 2

    Organism-specific databases

    GrameneiP14656.

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall Source: EnsemblPlants/Gramene
    2. cytosolic ribosome Source: EnsemblPlants/Gramene
    3. plasma membrane Source: EnsemblPlants/Gramene
    4. plasmodesma Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    Plants show marked reduction in leaf blades elongation, plant height, panicle size and grain filling.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Glutamine synthetase cytosolic isozyme 1-1PRO_0000153187Add
    BLAST

    Proteomic databases

    PaxDbiP14656.
    PRIDEiP14656.

    Expressioni

    Tissue specificityi

    Highly expressed in leaf blades, at intermediate levels in spikelets (rice flower) and at lower levels in roots.2 Publications

    Developmental stagei

    Expressed in metaxylem and phloem of parenchyma cells and in sclerenchyma cells of developing leaf blades.1 Publication

    Inductioni

    By ammonium supply under nitrogen-limited condition.1 Publication

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Protein-protein interaction databases

    IntActiP14656. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP14656.
    SMRiP14656. Positions 4-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    KOiK01915.
    OMAiHETSNIF.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14656-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASLTDLVNL NLSDTTEKII AEYIWIGGSG MDLRSKARTL SGPVTDPSKL    50
    PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRKGNNILV MCDCYTPAGE 100
    PIPTNKRHNA AKIFSSPEVA SEEPWYGIEQ EYTLLQKDIN WPLGWPVGGF 150
    PGPQGPYYCG IGADKSFGRD IVDSHYKACL YAGINISGIN GEVMPGQWEF 200
    QVGPSVGISA GDQVWVARYI LERITEIAGV VVSFDPKPIP GDWNGAGAHT 250
    NYSTKSMRND GGYEIIKSAI EKLKLRHKEH ISAYGEGNER RLTGRHETAD 300
    INTFSWGVAN RGASVRVGRE TEQNGKGYFE DRRPASNMDP YIVTSMIAET 350
    TIIWKP 356
    Length:356
    Mass (Da):39,201
    Last modified:April 1, 1990 - v1
    Checksum:i7D21F7C31B7CB4DD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14245 mRNA. Translation: CAA32461.1.
    AB037595 mRNA. Translation: BAA95679.1.
    AB037664 Genomic DNA. Translation: BAA95678.1.
    AP004880 Genomic DNA. Translation: BAD15892.1.
    PIRiS07470. AJRZQG.
    RefSeqiNP_001048045.1. NM_001054580.1.
    UniGeneiOs.7909.

    Genome annotation databases

    EnsemblPlantsiOS02T0735200-01; OS02T0735200-01; OS02G0735200.
    OS02T0735200-02; OS02T0735200-02; OS02G0735200.
    OS02T0735200-03; OS02T0735200-03; OS02G0735200.
    GeneIDi4330649.
    KEGGiosa:4330649.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14245 mRNA. Translation: CAA32461.1 .
    AB037595 mRNA. Translation: BAA95679.1 .
    AB037664 Genomic DNA. Translation: BAA95678.1 .
    AP004880 Genomic DNA. Translation: BAD15892.1 .
    PIRi S07470. AJRZQG.
    RefSeqi NP_001048045.1. NM_001054580.1.
    UniGenei Os.7909.

    3D structure databases

    ProteinModelPortali P14656.
    SMRi P14656. Positions 4-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P14656. 1 interaction.

    Proteomic databases

    PaxDbi P14656.
    PRIDEi P14656.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS02T0735200-01 ; OS02T0735200-01 ; OS02G0735200 .
    OS02T0735200-02 ; OS02T0735200-02 ; OS02G0735200 .
    OS02T0735200-03 ; OS02T0735200-03 ; OS02G0735200 .
    GeneIDi 4330649.
    KEGGi osa:4330649.

    Organism-specific databases

    Gramenei P14656.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    KOi K01915.
    OMAi HETSNIF.

    Enzyme and pathway databases

    BRENDAi 6.3.1.2. 4460.
    SABIO-RK P14656.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
      Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
      Plant Mol. Biol. 13:611-614(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Kinmaze.
      Tissue: Shoot.
    2. "Nucleotide sequence of a genomic DNA and a cDNA encoding cytosolic glutamine synthetase in Sasanishiki, a seading cultivar of rice (Oryza sativa L) in northern Japan."
      Kojima S., Hanzawa S., Hayakawa T., Hayashi M., Yamaya T.
      Plant Gene Register PGR00-048
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: cv. Sasanishiki.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
      Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
      Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 204-213.
      Strain: cv. Nipponbare.
      Tissue: Callus.
    5. "Overlapping expression of cytosolic glutamine synthetase and phenylalanine ammonia-lyase in immature leaf blades of rice."
      Sakurai N., Katayama Y., Yamaya T.
      Physiol. Plantarum 113:400-408(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    6. "Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots."
      Ishiyama K., Inoue E., Tabuchi M., Yamaya T., Takahashi H.
      Plant Cell Physiol. 45:1640-1647(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    7. "Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1."
      Tabuchi M., Sugiyama K., Ishiyama K., Inoue E., Sato T., Takahashi H., Yamaya T.
      Plant J. 42:641-651(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, INDUCTION.

    Entry informationi

    Entry nameiGLN11_ORYSJ
    AccessioniPrimary (citable) accession number: P14656
    Secondary accession number(s): Q6Z753
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3