SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P14655

- GLNA2_ORYSJ

UniProt

P14655 - GLNA2_ORYSJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glutamine synthetase, chloroplastic

Gene
GLN2, RGS31, Os04g0659100, LOC_Os04g56400, OSJNBa0011F23.15, OSJNBa0015K02.2
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. response to cadmium ion Source: EnsemblPlants/Gramene
  3. response to salt stress Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase, chloroplastic (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
OsGS2
Short name:
GS2
Gene namesi
Name:GLN2
Synonyms:RGS31
Ordered Locus Names:Os04g0659100, LOC_Os04g56400
ORF Names:OSJNBa0011F23.15, OSJNBa0015K02.2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 4

Organism-specific databases

GrameneiP14655.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: EnsemblPlants/Gramene
  2. chloroplast envelope Source: EnsemblPlants/Gramene
  3. chloroplast stroma Source: EnsemblPlants/Gramene
  4. chloroplast thylakoid membrane Source: EnsemblPlants/Gramene
  5. cytosolic ribosome Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848Chloroplast1 PublicationAdd
BLAST
Chaini49 – 428380Glutamine synthetase, chloroplasticPRO_0000011181Add
BLAST

Proteomic databases

PaxDbiP14655.
PRIDEiP14655.

Interactioni

Subunit structurei

Homooctamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP14655.
SMRiP14655. Positions 60-411.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
KOiK01915.
OMAiVVPAMQC.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P14655-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAQAVVPAMQ CQVGAVRARP AAAAAAAGGR VWGVRRTGRG TSGFRVMAVS    50
TETTGVVTRM EQLLNMDTTP FTDKIIAEYI WVGGTGIDLR SKSRTISKPV 100
EDPSELPKWN YDGSSTGQAP GEDSEVILYP QAIFKDPFRG GNNILVMCDT 150
YTPAGEPIPT NKRNRAAQVF SDPKVVSQVP WFGIEQEYTL LQRDVNWPLG 200
WPVGGYPGPQ GPYYCAVGSD KSFGRDISDA HYKACLYAGI NISGTNGEVM 250
PGQWEYQVGP SVGIEAGDHI WISRYILERI TEQAGVVLTL DPKPIQGDWN 300
GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHDLHISAY GEGNERRLTG 350
LHETASIDNF SWGVANRGCS IRVGRDTEAK GKGYLEDRRP ASNMDPYVVT 400
ALLAETTILW EPTLEAEVLA AKKLALKV 428
Length:428
Mass (Da):46,643
Last modified:April 1, 1990 - v1
Checksum:iDFF1B39BFC5921FE
GO
Isoform 2 (identifier: P14655-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     309-322: STKSMREDGGFEVI → RFHYLLLMLKYPAA
     323-428: Missing.

Note: No experimental confirmation available.

Show »
Length:322
Mass (Da):35,138
Checksum:i95B248B5835571F7
GO

Sequence cautioni

The sequence AAL87183.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei309 – 32214STKSM…GFEVI → RFHYLLLMLKYPAA in isoform 2. VSP_018231Add
BLAST
Alternative sequencei323 – 428106Missing in isoform 2. VSP_018232Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14246 mRNA. Translation: CAA32462.1.
AF480497 Genomic DNA. Translation: AAL87183.1. Sequence problems.
AL606608 Genomic DNA. Translation: CAE02885.2.
AL662953 Genomic DNA. Translation: CAE54574.1.
AK063706 mRNA. No translation available.
PIRiS07471. AJRZQD.
RefSeqiNP_001054133.1. NM_001060668.1. [P14655-1]
UniGeneiOs.19294.
Os.51574.

Genome annotation databases

EnsemblPlantsiOS04T0659100-01; OS04T0659100-01; OS04G0659100. [P14655-1]
OS04T0659100-03; OS04T0659100-03; OS04G0659100. [P14655-1]
GeneIDi4337272.
KEGGiosa:4337272.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14246 mRNA. Translation: CAA32462.1 .
AF480497 Genomic DNA. Translation: AAL87183.1 . Sequence problems.
AL606608 Genomic DNA. Translation: CAE02885.2 .
AL662953 Genomic DNA. Translation: CAE54574.1 .
AK063706 mRNA. No translation available.
PIRi S07471. AJRZQD.
RefSeqi NP_001054133.1. NM_001060668.1. [P14655-1 ]
UniGenei Os.19294.
Os.51574.

3D structure databases

ProteinModelPortali P14655.
SMRi P14655. Positions 60-411.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P14655.
PRIDEi P14655.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS04T0659100-01 ; OS04T0659100-01 ; OS04G0659100 . [P14655-1 ]
OS04T0659100-03 ; OS04T0659100-03 ; OS04G0659100 . [P14655-1 ]
GeneIDi 4337272.
KEGGi osa:4337272.

Organism-specific databases

Gramenei P14655.

Phylogenomic databases

eggNOGi COG0174.
HOGENOMi HOG000061500.
KOi K01915.
OMAi VVPAMQC.

Family and domain databases

Gene3Di 3.30.590.10. 1 hit.
InterProi IPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view ]
Pfami PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view ]
SUPFAMi SSF54368. SSF54368. 1 hit.
PROSITEi PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
    Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
    Plant Mol. Biol. 13:611-614(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Kinmaze.
    Tissue: Shoot.
  2. "Further evidence of microcolinearity between barley and rice genomes at two orthologous regions."
    Park Y.-J., Dixit A., Yoo J.-W., Bennetzen J.
    Mol. Cells 17:492-502(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Nipponbare.
  5. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
    Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
    Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 49-58.
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  6. "Enhanced tolerance to salt stress in transgenic rice that overexpresses chloroplast glutamine synthetase."
    Hoshida H., Tanaka Y., Hibino T., Hayashi Y., Tanaka A., Takabe T., Takabe T.
    Plant Mol. Biol. 43:103-111(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGLNA2_ORYSJ
AccessioniPrimary (citable) accession number: P14655
Secondary accession number(s): Q7XR09, Q8S3P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 14, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing GLN2 show enhanced photorespiration capacity.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi