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P14655 (GLNA2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase, chloroplastic

EC=6.3.1.2
Alternative name(s):
Glutamate--ammonia ligase
OsGS2
Short name=GS2
Gene names
Name:GLN2
Synonyms:RGS31
Ordered Locus Names:Os04g0659100, LOC_Os04g56400
ORF Names:OSJNBa0011F23.15, OSJNBa0015K02.2
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration. Ref.6

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Plastidchloroplast.

Miscellaneous

Plants overexpressing GLN2 show enhanced photorespiration capacity.

Sequence similarities

Belongs to the glutamine synthetase family.

Sequence caution

The sequence AAL87183.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

response to cadmium ion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to salt stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentapoplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast envelope

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast stroma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast thylakoid membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytosolic ribosome

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P14655-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P14655-2)

The sequence of this isoform differs from the canonical sequence as follows:
     309-322: STKSMREDGGFEVI → RFHYLLLMLKYPAA
     323-428: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4848Chloroplast Ref.5
Chain49 – 428380Glutamine synthetase, chloroplastic
PRO_0000011181

Natural variations

Alternative sequence309 – 32214STKSM…GFEVI → RFHYLLLMLKYPAA in isoform 2.
VSP_018231
Alternative sequence323 – 428106Missing in isoform 2.
VSP_018232

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: DFF1B39BFC5921FE

FASTA42846,643
        10         20         30         40         50         60 
MAQAVVPAMQ CQVGAVRARP AAAAAAAGGR VWGVRRTGRG TSGFRVMAVS TETTGVVTRM 

        70         80         90        100        110        120 
EQLLNMDTTP FTDKIIAEYI WVGGTGIDLR SKSRTISKPV EDPSELPKWN YDGSSTGQAP 

       130        140        150        160        170        180 
GEDSEVILYP QAIFKDPFRG GNNILVMCDT YTPAGEPIPT NKRNRAAQVF SDPKVVSQVP 

       190        200        210        220        230        240 
WFGIEQEYTL LQRDVNWPLG WPVGGYPGPQ GPYYCAVGSD KSFGRDISDA HYKACLYAGI 

       250        260        270        280        290        300 
NISGTNGEVM PGQWEYQVGP SVGIEAGDHI WISRYILERI TEQAGVVLTL DPKPIQGDWN 

       310        320        330        340        350        360 
GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHDLHISAY GEGNERRLTG LHETASIDNF 

       370        380        390        400        410        420 
SWGVANRGCS IRVGRDTEAK GKGYLEDRRP ASNMDPYVVT ALLAETTILW EPTLEAEVLA 


AKKLALKV 

« Hide

Isoform 2 [UniParc].

Checksum: 95B248B5835571F7
Show »

FASTA32235,138

References

« Hide 'large scale' references
[1]"Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
Plant Mol. Biol. 13:611-614(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: cv. Kinmaze.
Tissue: Shoot.
[2]"Further evidence of microcolinearity between barley and rice genomes at two orthologous regions."
Park Y.-J., Dixit A., Yoo J.-W., Bennetzen J.
Mol. Cells 17:492-502(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Sequence and analysis of rice chromosome 4."
Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. expand/collapse author list , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B.
Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Nipponbare.
[5]"Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 49-58.
Strain: cv. Nipponbare.
Tissue: Leaf.
[6]"Enhanced tolerance to salt stress in transgenic rice that overexpresses chloroplast glutamine synthetase."
Hoshida H., Tanaka Y., Hibino T., Hayashi Y., Tanaka A., Takabe T., Takabe T.
Plant Mol. Biol. 43:103-111(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14246 mRNA. Translation: CAA32462.1.
AF480497 Genomic DNA. Translation: AAL87183.1. Sequence problems.
AL606608 Genomic DNA. Translation: CAE02885.2.
AL662953 Genomic DNA. Translation: CAE54574.1.
AK063706 mRNA. No translation available.
PIRAJRZQD. S07471.
RefSeqNP_001054133.1. NM_001060668.1.
UniGeneOs.19294.
Os.51574.

3D structure databases

ProteinModelPortalP14655.
SMRP14655. Positions 60-411.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbP14655.
PRIDEP14655.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS04T0659100-01; OS04T0659100-01; OS04G0659100. [P14655-1]
OS04T0659100-03; OS04T0659100-03; OS04G0659100. [P14655-1]
GeneID4337272.
KEGGosa:4337272.

Organism-specific databases

GrameneP14655.

Phylogenomic databases

eggNOGCOG0174.
HOGENOMHOG000061500.
KOK01915.
OMAIEEIPEW.
ProtClustDBPLN03036.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA2_ORYSJ
AccessionPrimary (citable) accession number: P14655
Secondary accession number(s): Q7XR09, Q8S3P5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations