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Protein

Glutamine synthetase, chloroplastic

Gene

GLN2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.1 Publication

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 4460.
ReactomeiREACT_283821. Amino acid synthesis and interconversion (transamination).
REACT_297751. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase, chloroplastic (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
OsGS2
Short name:
GS2
Gene namesi
Name:GLN2
Synonyms:RGS31
Ordered Locus Names:Os04g0659100, LOC_Os04g56400
ORF Names:OSJNBa0011F23.15, OSJNBa0015K02.2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
ProteomesiUP000000763 Componenti: Chromosome 4

Organism-specific databases

GrameneiP14655.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4848Chloroplast1 PublicationAdd
BLAST
Chaini49 – 428380Glutamine synthetase, chloroplasticPRO_0000011181Add
BLAST

Proteomic databases

PaxDbiP14655.
PRIDEiP14655.

Expressioni

Gene expression databases

ExpressionAtlasiP14655. baseline and differential.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os04g56400.1.

Structurei

3D structure databases

ProteinModelPortaliP14655.
SMRiP14655. Positions 60-411.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiP14655.
KOiK01915.
OMAiSKRHDEH.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P14655-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQAVVPAMQ CQVGAVRARP AAAAAAAGGR VWGVRRTGRG TSGFRVMAVS
60 70 80 90 100
TETTGVVTRM EQLLNMDTTP FTDKIIAEYI WVGGTGIDLR SKSRTISKPV
110 120 130 140 150
EDPSELPKWN YDGSSTGQAP GEDSEVILYP QAIFKDPFRG GNNILVMCDT
160 170 180 190 200
YTPAGEPIPT NKRNRAAQVF SDPKVVSQVP WFGIEQEYTL LQRDVNWPLG
210 220 230 240 250
WPVGGYPGPQ GPYYCAVGSD KSFGRDISDA HYKACLYAGI NISGTNGEVM
260 270 280 290 300
PGQWEYQVGP SVGIEAGDHI WISRYILERI TEQAGVVLTL DPKPIQGDWN
310 320 330 340 350
GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHDLHISAY GEGNERRLTG
360 370 380 390 400
LHETASIDNF SWGVANRGCS IRVGRDTEAK GKGYLEDRRP ASNMDPYVVT
410 420
ALLAETTILW EPTLEAEVLA AKKLALKV
Length:428
Mass (Da):46,643
Last modified:April 1, 1990 - v1
Checksum:iDFF1B39BFC5921FE
GO
Isoform 2 (identifier: P14655-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     309-322: STKSMREDGGFEVI → RFHYLLLMLKYPAA
     323-428: Missing.

Note: No experimental confirmation available.
Show »
Length:322
Mass (Da):35,138
Checksum:i95B248B5835571F7
GO

Sequence cautioni

The sequence AAL87183.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei309 – 32214STKSM…GFEVI → RFHYLLLMLKYPAA in isoform 2. 1 PublicationVSP_018231Add
BLAST
Alternative sequencei323 – 428106Missing in isoform 2. 1 PublicationVSP_018232Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14246 mRNA. Translation: CAA32462.1.
AF480497 Genomic DNA. Translation: AAL87183.1. Sequence problems.
AL606608 Genomic DNA. Translation: CAE02885.2.
AL662953 Genomic DNA. Translation: CAE54574.1.
AK063706 mRNA. No translation available.
PIRiS07471. AJRZQD.
RefSeqiNP_001054133.1. NM_001060668.1. [P14655-1]
UniGeneiOs.19294.
Os.51574.

Genome annotation databases

EnsemblPlantsiOS04T0659100-01; OS04T0659100-01; OS04G0659100. [P14655-1]
OS04T0659100-03; OS04T0659100-03; OS04G0659100. [P14655-1]
GeneIDi4337272.
KEGGiosa:4337272.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14246 mRNA. Translation: CAA32462.1.
AF480497 Genomic DNA. Translation: AAL87183.1. Sequence problems.
AL606608 Genomic DNA. Translation: CAE02885.2.
AL662953 Genomic DNA. Translation: CAE54574.1.
AK063706 mRNA. No translation available.
PIRiS07471. AJRZQD.
RefSeqiNP_001054133.1. NM_001060668.1. [P14655-1]
UniGeneiOs.19294.
Os.51574.

3D structure databases

ProteinModelPortaliP14655.
SMRiP14655. Positions 60-411.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os04g56400.1.

Proteomic databases

PaxDbiP14655.
PRIDEiP14655.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS04T0659100-01; OS04T0659100-01; OS04G0659100. [P14655-1]
OS04T0659100-03; OS04T0659100-03; OS04G0659100. [P14655-1]
GeneIDi4337272.
KEGGiosa:4337272.

Organism-specific databases

GrameneiP14655.

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiP14655.
KOiK01915.
OMAiSKRHDEH.

Enzyme and pathway databases

BRENDAi6.3.1.2. 4460.
ReactomeiREACT_283821. Amino acid synthesis and interconversion (transamination).
REACT_297751. Astrocytic Glutamate-Glutamine Uptake And Metabolism.

Gene expression databases

ExpressionAtlasiP14655. baseline and differential.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
    Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
    Plant Mol. Biol. 13:611-614(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Kinmaze.
    Tissue: Shoot.
  2. "Further evidence of microcolinearity between barley and rice genomes at two orthologous regions."
    Park Y.-J., Dixit A., Yoo J.-W., Bennetzen J.
    Mol. Cells 17:492-502(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Nipponbare.
  5. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
    Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
    Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 49-58.
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  6. "Enhanced tolerance to salt stress in transgenic rice that overexpresses chloroplast glutamine synthetase."
    Hoshida H., Tanaka Y., Hibino T., Hayashi Y., Tanaka A., Takabe T., Takabe T.
    Plant Mol. Biol. 43:103-111(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiGLNA2_ORYSJ
AccessioniPrimary (citable) accession number: P14655
Secondary accession number(s): Q7XR09, Q8S3P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: June 24, 2015
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants overexpressing GLN2 show enhanced photorespiration capacity.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.