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P14655

- GLNA2_ORYSJ

UniProt

P14655 - GLNA2_ORYSJ

Protein

Glutamine synthetase, chloroplastic

Gene

GLN2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.1 Publication

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro
    2. response to cadmium ion Source: EnsemblPlants/Gramene
    3. response to salt stress Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase, chloroplastic (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase
    OsGS2
    Short name:
    GS2
    Gene namesi
    Name:GLN2
    Synonyms:RGS31
    Ordered Locus Names:Os04g0659100, LOC_Os04g56400
    ORF Names:OSJNBa0011F23.15, OSJNBa0015K02.2
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 4

    Organism-specific databases

    GrameneiP14655.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: EnsemblPlants/Gramene
    2. chloroplast envelope Source: EnsemblPlants/Gramene
    3. chloroplast stroma Source: EnsemblPlants/Gramene
    4. chloroplast thylakoid membrane Source: EnsemblPlants/Gramene
    5. cytosolic ribosome Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4848Chloroplast1 PublicationAdd
    BLAST
    Chaini49 – 428380Glutamine synthetase, chloroplasticPRO_0000011181Add
    BLAST

    Proteomic databases

    PaxDbiP14655.
    PRIDEiP14655.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP14655.
    SMRiP14655. Positions 60-411.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    KOiK01915.
    OMAiVVPAMQC.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P14655-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAQAVVPAMQ CQVGAVRARP AAAAAAAGGR VWGVRRTGRG TSGFRVMAVS    50
    TETTGVVTRM EQLLNMDTTP FTDKIIAEYI WVGGTGIDLR SKSRTISKPV 100
    EDPSELPKWN YDGSSTGQAP GEDSEVILYP QAIFKDPFRG GNNILVMCDT 150
    YTPAGEPIPT NKRNRAAQVF SDPKVVSQVP WFGIEQEYTL LQRDVNWPLG 200
    WPVGGYPGPQ GPYYCAVGSD KSFGRDISDA HYKACLYAGI NISGTNGEVM 250
    PGQWEYQVGP SVGIEAGDHI WISRYILERI TEQAGVVLTL DPKPIQGDWN 300
    GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHDLHISAY GEGNERRLTG 350
    LHETASIDNF SWGVANRGCS IRVGRDTEAK GKGYLEDRRP ASNMDPYVVT 400
    ALLAETTILW EPTLEAEVLA AKKLALKV 428
    Length:428
    Mass (Da):46,643
    Last modified:April 1, 1990 - v1
    Checksum:iDFF1B39BFC5921FE
    GO
    Isoform 2 (identifier: P14655-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         309-322: STKSMREDGGFEVI → RFHYLLLMLKYPAA
         323-428: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:322
    Mass (Da):35,138
    Checksum:i95B248B5835571F7
    GO

    Sequence cautioni

    The sequence AAL87183.1 differs from that shown. Reason: Erroneous gene model prediction.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei309 – 32214STKSM…GFEVI → RFHYLLLMLKYPAA in isoform 2. 1 PublicationVSP_018231Add
    BLAST
    Alternative sequencei323 – 428106Missing in isoform 2. 1 PublicationVSP_018232Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14246 mRNA. Translation: CAA32462.1.
    AF480497 Genomic DNA. Translation: AAL87183.1. Sequence problems.
    AL606608 Genomic DNA. Translation: CAE02885.2.
    AL662953 Genomic DNA. Translation: CAE54574.1.
    AK063706 mRNA. No translation available.
    PIRiS07471. AJRZQD.
    RefSeqiNP_001054133.1. NM_001060668.1. [P14655-1]
    UniGeneiOs.19294.
    Os.51574.

    Genome annotation databases

    EnsemblPlantsiOS04T0659100-01; OS04T0659100-01; OS04G0659100. [P14655-1]
    OS04T0659100-03; OS04T0659100-03; OS04G0659100. [P14655-1]
    GeneIDi4337272.
    KEGGiosa:4337272.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14246 mRNA. Translation: CAA32462.1 .
    AF480497 Genomic DNA. Translation: AAL87183.1 . Sequence problems.
    AL606608 Genomic DNA. Translation: CAE02885.2 .
    AL662953 Genomic DNA. Translation: CAE54574.1 .
    AK063706 mRNA. No translation available.
    PIRi S07471. AJRZQD.
    RefSeqi NP_001054133.1. NM_001060668.1. [P14655-1 ]
    UniGenei Os.19294.
    Os.51574.

    3D structure databases

    ProteinModelPortali P14655.
    SMRi P14655. Positions 60-411.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P14655.
    PRIDEi P14655.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS04T0659100-01 ; OS04T0659100-01 ; OS04G0659100 . [P14655-1 ]
    OS04T0659100-03 ; OS04T0659100-03 ; OS04G0659100 . [P14655-1 ]
    GeneIDi 4337272.
    KEGGi osa:4337272.

    Organism-specific databases

    Gramenei P14655.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    KOi K01915.
    OMAi VVPAMQC.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
      Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
      Plant Mol. Biol. 13:611-614(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: cv. Kinmaze.
      Tissue: Shoot.
    2. "Further evidence of microcolinearity between barley and rice genomes at two orthologous regions."
      Park Y.-J., Dixit A., Yoo J.-W., Bennetzen J.
      Mol. Cells 17:492-502(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: cv. Nipponbare.
    5. "Rice proteome database based on two-dimensional polyacrylamide gel electrophoresis: its status in 2003."
      Komatsu S., Kojima K., Suzuki K., Ozaki K., Higo K.
      Nucleic Acids Res. 32:D388-D392(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 49-58.
      Strain: cv. Nipponbare.
      Tissue: Leaf.
    6. "Enhanced tolerance to salt stress in transgenic rice that overexpresses chloroplast glutamine synthetase."
      Hoshida H., Tanaka Y., Hibino T., Hayashi Y., Tanaka A., Takabe T., Takabe T.
      Plant Mol. Biol. 43:103-111(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGLNA2_ORYSJ
    AccessioniPrimary (citable) accession number: P14655
    Secondary accession number(s): Q7XR09, Q8S3P5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plants overexpressing GLN2 show enhanced photorespiration capacity.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3