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P14654

- GLN12_ORYSJ

UniProt

P14654 - GLN12_ORYSJ

Protein

Glutamine synthetase cytosolic isozyme 1-2

Gene

GLN1-2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Apr 1990)
      Previous versions | rss
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    Functioni

    High-affinity glutamine synthetase. May play a role in the primary assimilation of ammonium taken up by roots.

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    Kineticsi

    Measured at pH 7.8 and 30 degrees Celsius for all experiments.

    1. KM=2.1 mM for glutamate1 Publication
    2. KM=73 µM for ammonium1 Publication
    3. KM=530 µM for ATP1 Publication

    Vmax=94.7 nmol/sec/mg enzyme with glutamate as substrate1 Publication

    Vmax=98.1 nmol/sec/mg enzyme with ammonium as substrate1 Publication

    Vmax=109.1 nmol/sec/mg enzyme with ATP as substrate1 Publication

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.3.1.2. 4460.
    SABIO-RKP14654.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme 1-2 (EC:6.3.1.2)
    Alternative name(s):
    Glutamate--ammonia ligase GLN1;2
    Short name:
    OsGLN1;2
    Glutamine synthetase root isozyme
    OsGS1;2
    Gene namesi
    Name:GLN1-2
    Synonyms:GSR, RGS8
    Ordered Locus Names:Os03g0223400, LOC_Os03g12290
    ORF Names:OJ1743A09.19
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 3

    Organism-specific databases

    GrameneiP14654.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: EnsemblPlants/Gramene
    2. plasmodesma Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 357357Glutamine synthetase cytosolic isozyme 1-2PRO_0000153186Add
    BLAST

    Proteomic databases

    PaxDbiP14654.

    Expressioni

    Tissue specificityi

    Expressed in roots and at lower levels in leaf blades and spikelets (rice flower).2 Publications

    Inductioni

    By ammonium supply under nitrogen-limited condition.1 Publication

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP14654.
    SMRiP14654. Positions 4-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0174.
    HOGENOMiHOG000061500.
    KOiK01915.
    OMAiKSFGRDV.

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14654-1 [UniParc]FASTAAdd to Basket

    « Hide

    MANLTDLVNL NLSDCSDKII AEYIWVGGSG IDLRSKARTV KGPITDVSQL    50
    PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGDNILV MCDCYTPQGE 100
    PIPTNKRHSA AKIFSHPDVV AEVPWYGIEQ EYTLLQKDVN WPLGWPVGGF 150
    PGPQGPYYCA AGAEKAFGRD IVDAHYKACI YAGINISGIN GEVMPGQWEF 200
    QVGPSVGIAA ADQVWVARYI LERVTEVAGV VLSLDPKPIP GDWNGAGAHT 250
    NFSTKSMREP GGYEVIKKAI DKLALRHKEH IAAYGEGNER RLTGRHETAD 300
    INTFKWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTGMIAET 350
    TLLWKQN 357
    Length:357
    Mass (Da):39,258
    Last modified:April 1, 1990 - v1
    Checksum:i2FF5634EDEF2E77E
    GO

    Sequence cautioni

    The sequence AAN05339.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14244 mRNA. Translation: CAA32460.1.
    AB180688 mRNA. Translation: BAD77931.1.
    AC105364 Genomic DNA. Translation: AAN05339.1. Sequence problems.
    PIRiS07469. AJRZQB.
    RefSeqiNP_001049424.1. NM_001055959.2.
    UniGeneiOs.12728.

    Genome annotation databases

    EnsemblPlantsiOS03T0223400-01; OS03T0223400-01; OS03G0223400.
    GeneIDi4332108.
    KEGGiosa:4332108.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14244 mRNA. Translation: CAA32460.1 .
    AB180688 mRNA. Translation: BAD77931.1 .
    AC105364 Genomic DNA. Translation: AAN05339.1 . Sequence problems.
    PIRi S07469. AJRZQB.
    RefSeqi NP_001049424.1. NM_001055959.2.
    UniGenei Os.12728.

    3D structure databases

    ProteinModelPortali P14654.
    SMRi P14654. Positions 4-355.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P14654.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS03T0223400-01 ; OS03T0223400-01 ; OS03G0223400 .
    GeneIDi 4332108.
    KEGGi osa:4332108.

    Organism-specific databases

    Gramenei P14654.

    Phylogenomic databases

    eggNOGi COG0174.
    HOGENOMi HOG000061500.
    KOi K01915.
    OMAi KSFGRDV.

    Enzyme and pathway databases

    BRENDAi 6.3.1.2. 4460.
    SABIO-RK P14654.

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
      Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
      Plant Mol. Biol. 13:611-614(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Kinmaze.
      Tissue: Root.
    2. "Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots."
      Ishiyama K., Inoue E., Tabuchi M., Yamaya T., Takahashi H.
      Plant Cell Physiol. 45:1640-1647(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Sasanishiki.
      Tissue: Root.
    3. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
      The rice chromosome 3 sequencing consortium
      Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
      , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
      Genome Res. 15:1284-1291(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1."
      Tabuchi M., Sugiyama K., Ishiyama K., Inoue E., Sato T., Takahashi H., Yamaya T.
      Plant J. 42:641-651(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGLN12_ORYSJ
    AccessioniPrimary (citable) accession number: P14654
    Secondary accession number(s): Q5NU22, Q8GTZ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: April 1, 1990
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3