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Protein

Glutamine synthetase cytosolic isozyme 1-2

Gene

GLN1-2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High-affinity glutamine synthetase. May play a role in the primary assimilation of ammonium taken up by roots.

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Kineticsi

Measured at pH 7.8 and 30 degrees Celsius for all experiments.

  1. KM=2.1 mM for glutamate1 Publication
  2. KM=73 µM for ammonium1 Publication
  3. KM=530 µM for ATP1 Publication

Vmax=94.7 nmol/sec/mg enzyme with glutamate as substrate1 Publication

Vmax=98.1 nmol/sec/mg enzyme with ammonium as substrate1 Publication

Vmax=109.1 nmol/sec/mg enzyme with ATP as substrate1 Publication

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.1.2. 4460.
ReactomeiREACT_283821. Amino acid synthesis and interconversion (transamination).
REACT_297751. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
SABIO-RKP14654.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1-2 (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase GLN1;2
Short name:
OsGLN1;2
Glutamine synthetase root isozyme
OsGS1;2
Gene namesi
Name:GLN1-2
Synonyms:GSR, RGS8
Ordered Locus Names:Os03g0223400, LOC_Os03g12290
ORF Names:OJ1743A09.19
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 3

Organism-specific databases

GrameneiP14654.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 357357Glutamine synthetase cytosolic isozyme 1-2PRO_0000153186Add
BLAST

Proteomic databases

PaxDbiP14654.

Expressioni

Tissue specificityi

Expressed in roots and at lower levels in leaf blades and spikelets (rice flower).2 Publications

Inductioni

By ammonium supply under nitrogen-limited condition.1 Publication

Gene expression databases

ExpressionAtlasiP14654. baseline.

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP14654.
SMRiP14654. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiP14654.
KOiK01915.
OMAiKKHNEHI.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANLTDLVNL NLSDCSDKII AEYIWVGGSG IDLRSKARTV KGPITDVSQL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEV ILYPQAIFKD PFRRGDNILV MCDCYTPQGE
110 120 130 140 150
PIPTNKRHSA AKIFSHPDVV AEVPWYGIEQ EYTLLQKDVN WPLGWPVGGF
160 170 180 190 200
PGPQGPYYCA AGAEKAFGRD IVDAHYKACI YAGINISGIN GEVMPGQWEF
210 220 230 240 250
QVGPSVGIAA ADQVWVARYI LERVTEVAGV VLSLDPKPIP GDWNGAGAHT
260 270 280 290 300
NFSTKSMREP GGYEVIKKAI DKLALRHKEH IAAYGEGNER RLTGRHETAD
310 320 330 340 350
INTFKWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTGMIAET

TLLWKQN
Length:357
Mass (Da):39,258
Last modified:March 31, 1990 - v1
Checksum:i2FF5634EDEF2E77E
GO

Sequence cautioni

The sequence AAN05339.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14244 mRNA. Translation: CAA32460.1.
AB180688 mRNA. Translation: BAD77931.1.
AC105364 Genomic DNA. Translation: AAN05339.1. Sequence problems.
PIRiS07469. AJRZQB.
RefSeqiNP_001049424.1. NM_001055959.2.
UniGeneiOs.12728.

Genome annotation databases

EnsemblPlantsiOS03T0223400-01; OS03T0223400-01; OS03G0223400.
GeneIDi4332108.
KEGGiosa:4332108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14244 mRNA. Translation: CAA32460.1.
AB180688 mRNA. Translation: BAD77931.1.
AC105364 Genomic DNA. Translation: AAN05339.1. Sequence problems.
PIRiS07469. AJRZQB.
RefSeqiNP_001049424.1. NM_001055959.2.
UniGeneiOs.12728.

3D structure databases

ProteinModelPortaliP14654.
SMRiP14654. Positions 4-355.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PaxDbiP14654.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS03T0223400-01; OS03T0223400-01; OS03G0223400.
GeneIDi4332108.
KEGGiosa:4332108.

Organism-specific databases

GrameneiP14654.

Phylogenomic databases

eggNOGiCOG0174.
HOGENOMiHOG000061500.
InParanoidiP14654.
KOiK01915.
OMAiKKHNEHI.

Enzyme and pathway databases

BRENDAi6.3.1.2. 4460.
ReactomeiREACT_283821. Amino acid synthesis and interconversion (transamination).
REACT_297751. Astrocytic Glutamate-Glutamine Uptake And Metabolism.
SABIO-RKP14654.

Gene expression databases

ExpressionAtlasiP14654. baseline.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Three cDNA sequences coding for glutamine synthetase polypeptides in Oryza sativa L."
    Sakamoto A., Ogawa M., Masumura T., Shibata D., Takeba G., Tanaka K., Fujii S.
    Plant Mol. Biol. 13:611-614(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Kinmaze.
    Tissue: Root.
  2. "Biochemical background and compartmentalized functions of cytosolic glutamine synthetase for active ammonium assimilation in rice roots."
    Ishiyama K., Inoue E., Tabuchi M., Yamaya T., Takahashi H.
    Plant Cell Physiol. 45:1640-1647(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Sasanishiki.
    Tissue: Root.
  3. "Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species."
    The rice chromosome 3 sequencing consortium
    Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S.
    , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S.
    Genome Res. 15:1284-1291(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Severe reduction in growth rate and grain filling of rice mutants lacking OsGS1;1, a cytosolic glutamine synthetase1;1."
    Tabuchi M., Sugiyama K., Ishiyama K., Inoue E., Sato T., Takahashi H., Yamaya T.
    Plant J. 42:641-651(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiGLN12_ORYSJ
AccessioniPrimary (citable) accession number: P14654
Secondary accession number(s): Q5NU22, Q8GTZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 31, 1990
Last sequence update: March 31, 1990
Last modified: March 31, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.