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P14650

- PERT_RAT

UniProt

P14650 - PERT_RAT

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Protein
Thyroid peroxidase
Gene
Tpo
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Catalytic activityi

2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.
[Thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O.
[Thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O.
2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O.
[Thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O.

Cofactori

Binds 1 calcium ion per heterodimer By similarity.
Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei232 – 2321Heme (covalent; via 2 links) By similarity
Active sitei233 – 2331Proton acceptor By similarity
Metal bindingi234 – 2341Calcium By similarity
Metal bindingi313 – 3131Calcium By similarity
Metal bindingi315 – 3151Calcium; via carbonyl oxygen By similarity
Metal bindingi317 – 3171Calcium By similarity
Metal bindingi319 – 3191Calcium By similarity
Sitei384 – 3841Transition state stabilizer By similarity
Binding sitei387 – 3871Heme (covalent; via 2 links) By similarity
Metal bindingi482 – 4821Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. heme binding Source: InterPro
  3. iodide peroxidase activity Source: RGD
Complete GO annotation...

GO - Biological processi

  1. cellular response to nitric oxide Source: RGD
  2. hormone biosynthetic process Source: UniProtKB-KW
  3. hydrogen peroxide catabolic process Source: UniProtKB-KW
  4. response to lipid Source: RGD
  5. thyroid hormone generation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide, Thyroid hormones biosynthesis

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00194.

Protein family/group databases

PeroxiBasei3973. RnoTPO.

Names & Taxonomyi

Protein namesi
Recommended name:
Thyroid peroxidase (EC:1.11.1.8)
Short name:
TPO
Gene namesi
Name:Tpo
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3900. Tpo.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 834803Extracellular Reviewed prediction
Add
BLAST
Transmembranei835 – 85925Helical; Reviewed prediction
Add
BLAST
Topological domaini860 – 91455Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. cell surface Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131 Reviewed prediction
Add
BLAST
Chaini32 – 914883Thyroid peroxidase
PRO_0000023665Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi123 – 1231N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi136 ↔ 152 By similarity
Disulfide bondi253 ↔ 263 By similarity
Disulfide bondi257 ↔ 278 By similarity
Glycosylationi271 – 2711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi299 – 2991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi334 – 3341N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi586 ↔ 643 By similarity
Glycosylationi603 – 6031N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi684 ↔ 709 By similarity
Disulfide bondi730 ↔ 770 By similarity
Disulfide bondi756 ↔ 782 By similarity
Disulfide bondi788 ↔ 802 By similarity
Disulfide bondi796 ↔ 811 By similarity
Disulfide bondi813 ↔ 826 By similarity

Post-translational modificationi

Heme is covalently bound through a H2O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface By similarity.
Cleaved in its N-terminal part By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP14650.
PRIDEiP14650.

Expressioni

Gene expression databases

GenevestigatoriP14650.

Interactioni

Subunit structurei

Interacts with DUOX1, DUOX2 and CYBA By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006526.

Structurei

3D structure databases

ProteinModelPortaliP14650.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini728 – 78356Sushi
Add
BLAST
Domaini784 – 82744EGF-like; calcium-binding Reviewed prediction
Add
BLAST

Sequence similaritiesi

Contains 1 EGF-like domain.

Keywords - Domaini

EGF-like domain, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG262194.
HOGENOMiHOG000016084.
HOVERGENiHBG000071.
InParanoidiP14650.
PhylomeDBiP14650.

Family and domain databases

Gene3Di1.10.640.10. 2 hits.
InterProiIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR000436. Sushi_SCR_CCP.
IPR029589. TPO.
[Graphical view]
PANTHERiPTHR11475:SF60. PTHR11475:SF60. 1 hit.
PfamiPF03098. An_peroxidase. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view]
PRINTSiPR00457. ANPEROXIDASE.
SMARTiSM00032. CCP. 1 hit.
SM00179. EGF_CA. 1 hit.
[Graphical view]
SUPFAMiSSF48113. SSF48113. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14650-1 [UniParc]FASTAAdd to Basket

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MRTLGAMAVM LVVMGTAIFL PFLLRSRDIL GGKTMTSHVI SVVETSQLLV    50
DNAVYNTMKR NLKKRGVLSP AQLLSFSKLP ESTSGAISRA AEIMETSIQV 100
MKREQSQFST DALSADILAT IANLSGCLPF MLPPRCPDTC LANKYRPITG 150
VCNNRDHPRW GASNTALARW LPPVYEDGFS QPRGWNPNFL YHGFPLPPVR 200
EVTRHLIQVS NEAVTEDDQY SDFLPVWGQY IDHDIALTPQ STSTAAFWGG 250
VDCQLTCENQ NPCFPIQLPS NSSRTTACLP FYRSSAACGT GDQGALFGNL 300
SAANPRQQMN GLTSFLDAST VYGSSPGVEK QLRNWSSSAG LLRVNTLHLD 350
SGRAYLPFAS AACAPEPGAP HANRTPCFLA GDGRASEVPA LAAVHTLWLR 400
EHNRLATAFK AINTHWSANT AYQEARKVVG ALHQIITMRD YIPKILGPDA 450
FRQYVGPYEG YNPTVNPTVS NVFSTAAFRF GHATVHPLVR RLNTDFQDHT 500
ELPRLQLHDV FFRPWRLIQE GGLDPIVRGL LARPAKLQVQ EQLMNEELTE 550
RLFVLSNVGT LDLASLNLQR GRDHGLPGYN EWREFCGLSR LDTGAELNKA 600
IANRSMVNKI MELYKHADNI DVWLGGLAEK FLPGARTGPL FACIIGKQMK 650
ALRDGDRFWW ENSHVFTDAQ RQELEKHSLP RVICDNTGLT RVPVDAFRIG 700
KFPQDFESCE EIPSMDLRLW RETFPQDDKC VFPEKVDNGN FVHCEESGKL 750
VLVYSCFHGY KLQGQEQVTC TQNGWDSEPP VCKDVNECAD LTHPPCHSSA 800
KCKNTKGSFQ CVCTDPYMLG EDEKTCIDSG RLPRASWVSI ALGALLIGGL 850
ASLSWTVICR WTHADKKSTL LITERVTMES GFRKSQESGI SPQKAEVQDA 900
EQEPAYGSRV LLCE 914
Length:914
Mass (Da):101,460
Last modified:April 1, 1990 - v1
Checksum:iB700B89439E85191
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1952FP → LG in AAA42265. 1 Publication
Sequence conflicti198 – 1981P → S in AAA42265. 1 Publication
Sequence conflicti228 – 2281G → A in AAA42265. 1 Publication
Sequence conflicti592 – 5943DTG → ETP in AAA42265. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17396 mRNA. Translation: CAA35257.1.
M31655 mRNA. Translation: AAA42265.1.
PIRiS07047.
UniGeneiRn.233736.

Genome annotation databases

UCSCiRGD:3900. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X17396 mRNA. Translation: CAA35257.1 .
M31655 mRNA. Translation: AAA42265.1 .
PIRi S07047.
UniGenei Rn.233736.

3D structure databases

ProteinModelPortali P14650.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000006526.

Protein family/group databases

PeroxiBasei 3973. RnoTPO.

Proteomic databases

PaxDbi P14650.
PRIDEi P14650.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:3900. rat.

Organism-specific databases

RGDi 3900. Tpo.

Phylogenomic databases

eggNOGi NOG262194.
HOGENOMi HOG000016084.
HOVERGENi HBG000071.
InParanoidi P14650.
PhylomeDBi P14650.

Enzyme and pathway databases

UniPathwayi UPA00194 .

Miscellaneous databases

NextBioi 610988.
PROi P14650.

Gene expression databases

Genevestigatori P14650.

Family and domain databases

Gene3Di 1.10.640.10. 2 hits.
InterProi IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR010255. Haem_peroxidase.
IPR019791. Haem_peroxidase_animal.
IPR000436. Sushi_SCR_CCP.
IPR029589. TPO.
[Graphical view ]
PANTHERi PTHR11475:SF60. PTHR11475:SF60. 1 hit.
Pfami PF03098. An_peroxidase. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view ]
PRINTSi PR00457. ANPEROXIDASE.
SMARTi SM00032. CCP. 1 hit.
SM00179. EGF_CA. 1 hit.
[Graphical view ]
SUPFAMi SSF48113. SSF48113. 1 hit.
SSF57535. SSF57535. 1 hit.
PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00435. PEROXIDASE_1. 1 hit.
PS50292. PEROXIDASE_3. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of the cDNA for thyroid peroxidase in FRTL5 rat thyroid cells."
    Derwahl M., Seto P., Rapoport B.
    Nucleic Acids Res. 17:8380-8380(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Thyroid peroxidase: rat cDNA sequence, chromosomal localization in mouse, and regulation of gene expression by comparison to thyroglobulin in rat FRTL-5 cells."
    Isozaki O., Kohn L.D., Kozak C.A., Kimura S.
    Mol. Endocrinol. 3:1681-1692(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 145-914.

Entry informationi

Entry nameiPERT_RAT
AccessioniPrimary (citable) accession number: P14650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: September 3, 2014
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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