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P14650 (PERT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thyroid peroxidase
Short name=TPO
EC=1.11.1.8
Gene names
Name:Tpo
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length914 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Catalytic activity

2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.

[Thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O.

[Thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O.

2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O.

[Thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O.

Cofactor

Binds 1 calcium ion per heterodimer By similarity.

Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer By similarity.

Pathway

Hormone biosynthesis; thyroid hormone biosynthesis.

Subunit structure

Interacts with DUOX1, DUOX2 and CYBA By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein By similarity.

Post-translational modification

Heme is covalently bound through a H2O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface By similarity.

Cleaved in its N-terminal part By similarity.

Sequence similarities

Belongs to the peroxidase family. XPO subfamily.

Contains 1 EGF-like domain.

Contains 1 Sushi (CCP/SCR) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 914883Thyroid peroxidase
PRO_0000023665

Regions

Topological domain32 – 834803Extracellular Potential
Transmembrane835 – 85925Helical; Potential
Topological domain860 – 91455Cytoplasmic Potential
Domain728 – 78356Sushi
Domain784 – 82744EGF-like; calcium-binding Potential

Sites

Active site2331Proton acceptor By similarity
Metal binding2341Calcium By similarity
Metal binding3131Calcium By similarity
Metal binding3151Calcium; via carbonyl oxygen By similarity
Metal binding3171Calcium By similarity
Metal binding3191Calcium By similarity
Metal binding4821Iron (heme axial ligand) By similarity
Binding site2321Heme (covalent; via 2 links) By similarity
Binding site3871Heme (covalent; via 2 links) By similarity
Site3841Transition state stabilizer By similarity

Amino acid modifications

Glycosylation1231N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation2991N-linked (GlcNAc...) Potential
Glycosylation3341N-linked (GlcNAc...) Potential
Glycosylation6031N-linked (GlcNAc...) Potential
Disulfide bond136 ↔ 152 By similarity
Disulfide bond253 ↔ 263 By similarity
Disulfide bond257 ↔ 278 By similarity
Disulfide bond586 ↔ 643 By similarity
Disulfide bond684 ↔ 709 By similarity
Disulfide bond730 ↔ 770 By similarity
Disulfide bond756 ↔ 782 By similarity
Disulfide bond788 ↔ 802 By similarity
Disulfide bond796 ↔ 811 By similarity
Disulfide bond813 ↔ 826 By similarity

Experimental info

Sequence conflict194 – 1952FP → LG in AAA42265. Ref.2
Sequence conflict1981P → S in AAA42265. Ref.2
Sequence conflict2281G → A in AAA42265. Ref.2
Sequence conflict592 – 5943DTG → ETP in AAA42265. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P14650 [UniParc].

Last modified April 1, 1990. Version 1.
Checksum: B700B89439E85191

FASTA914101,460
        10         20         30         40         50         60 
MRTLGAMAVM LVVMGTAIFL PFLLRSRDIL GGKTMTSHVI SVVETSQLLV DNAVYNTMKR 

        70         80         90        100        110        120 
NLKKRGVLSP AQLLSFSKLP ESTSGAISRA AEIMETSIQV MKREQSQFST DALSADILAT 

       130        140        150        160        170        180 
IANLSGCLPF MLPPRCPDTC LANKYRPITG VCNNRDHPRW GASNTALARW LPPVYEDGFS 

       190        200        210        220        230        240 
QPRGWNPNFL YHGFPLPPVR EVTRHLIQVS NEAVTEDDQY SDFLPVWGQY IDHDIALTPQ 

       250        260        270        280        290        300 
STSTAAFWGG VDCQLTCENQ NPCFPIQLPS NSSRTTACLP FYRSSAACGT GDQGALFGNL 

       310        320        330        340        350        360 
SAANPRQQMN GLTSFLDAST VYGSSPGVEK QLRNWSSSAG LLRVNTLHLD SGRAYLPFAS 

       370        380        390        400        410        420 
AACAPEPGAP HANRTPCFLA GDGRASEVPA LAAVHTLWLR EHNRLATAFK AINTHWSANT 

       430        440        450        460        470        480 
AYQEARKVVG ALHQIITMRD YIPKILGPDA FRQYVGPYEG YNPTVNPTVS NVFSTAAFRF 

       490        500        510        520        530        540 
GHATVHPLVR RLNTDFQDHT ELPRLQLHDV FFRPWRLIQE GGLDPIVRGL LARPAKLQVQ 

       550        560        570        580        590        600 
EQLMNEELTE RLFVLSNVGT LDLASLNLQR GRDHGLPGYN EWREFCGLSR LDTGAELNKA 

       610        620        630        640        650        660 
IANRSMVNKI MELYKHADNI DVWLGGLAEK FLPGARTGPL FACIIGKQMK ALRDGDRFWW 

       670        680        690        700        710        720 
ENSHVFTDAQ RQELEKHSLP RVICDNTGLT RVPVDAFRIG KFPQDFESCE EIPSMDLRLW 

       730        740        750        760        770        780 
RETFPQDDKC VFPEKVDNGN FVHCEESGKL VLVYSCFHGY KLQGQEQVTC TQNGWDSEPP 

       790        800        810        820        830        840 
VCKDVNECAD LTHPPCHSSA KCKNTKGSFQ CVCTDPYMLG EDEKTCIDSG RLPRASWVSI 

       850        860        870        880        890        900 
ALGALLIGGL ASLSWTVICR WTHADKKSTL LITERVTMES GFRKSQESGI SPQKAEVQDA 

       910 
EQEPAYGSRV LLCE

« Hide

References

[1]"Complete nucleotide sequence of the cDNA for thyroid peroxidase in FRTL5 rat thyroid cells."
Derwahl M., Seto P., Rapoport B.
Nucleic Acids Res. 17:8380-8380(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Thyroid peroxidase: rat cDNA sequence, chromosomal localization in mouse, and regulation of gene expression by comparison to thyroglobulin in rat FRTL-5 cells."
Isozaki O., Kohn L.D., Kozak C.A., Kimura S.
Mol. Endocrinol. 3:1681-1692(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 145-914.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X17396 mRNA. Translation: CAA35257.1.
M31655 mRNA. Translation: AAA42265.1.
IPIIPI00207331.
PIRS07047.
UniGeneRn.233736.

3D structure databases

ProteinModelPortalP14650.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000006526.

Protein family/group databases

PeroxiBase3973. RnoTPO.

Proteomic databases

PaxDbP14650.
PRIDEP14650.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:3900. rat.

Organism-specific databases

RGD3900. Tpo.

Phylogenomic databases

eggNOGNOG262194.
HOGENOMHOG000016084.
HOVERGENHBG000071.
InParanoidP14650.
OrthoDBEOG415GD3.

Enzyme and pathway databases

UniPathwayUPA00194.

Gene expression databases

ArrayExpressP14650.
GenevestigatorP14650.
GermOnlineENSRNOG00000004646. Rattus norvegicus.

Family and domain databases

Gene3D1.10.640.10. 2 hits.
InterProIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR010255. Haem_peroxidase.
IPR002007. Haem_peroxidase_animal.
IPR019791. Haem_peroxidase_animal_subgr.
IPR000436. Sushi_SCR_CCP.
[Graphical view]
PfamPF03098. An_peroxidase. 1 hit.
PF07645. EGF_CA. 1 hit.
PF00084. Sushi. 1 hit.
[Graphical view]
PRINTSPR00457. ANPEROXIDASE.
SMARTSM00032. CCP. 1 hit.
SM00179. EGF_CA. 1 hit.
[Graphical view]
SUPFAMSSF57535. Complement_control_module. 1 hit.
SSF48113. Peroxidase_super. 1 hit.
PROSITEPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. False negative.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. False negative.
PS50292. PEROXIDASE_3. 1 hit.
PS50923. SUSHI. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio610988.

Entry information

Entry namePERT_RAT
AccessionPrimary (citable) accession number: P14650
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: May 29, 2013
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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