P14649 (MYL6B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin light chain 6B

Alternative name(s):
Myosin light chain 1 slow-twitch muscle A isoform
Short name=MLC1sa
Smooth muscle and nonmuscle myosin light chain alkali 6B

Gene names

Name:	MYL6B
Synonyms:	MLC1SA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory light chain of myosin. Does not bind calcium.
Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains.
Sequence similarities	Contains 3 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Molecular function	Motor protein Muscle protein Myosin
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	muscle filament sliding Traceable author statement Ref.1. Source: HGNC skeletal muscle tissue development Traceable author statement Ref.1. Source: HGNC
Cellular_component	cytosol Traceable author statement. Source: Reactome muscle myosin complex Traceable author statement PubMed 2458299. Source: ProtInc unconventional myosin complex Traceable author statement Ref.1. Source: BHF-UCL
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro motor activity Traceable author statement Ref.1. Source: HGNC structural constituent of muscle Traceable author statement Ref.1. Source: HGNC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
MYO5A	Q02440	2	EBI-358570,EBI-1040586	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 208

208

Myosin light chain 6B

PRO_0000198695

Regions

Domain

64 – 99

36

EF-hand 1

Domain

141 – 176

36

EF-hand 2

Domain

176 – 208

33

EF-hand 3

Secondary structure

1

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208

Helix Strand Turn

Details...

Helix

69 – 76

8

Beta strand

79 – 86

8

Helix

89 – 95

7

Helix

102 – 108

7

Helix

114 – 117

4

Beta strand

121 – 123

3

Helix

124 – 134

11

Helix

147 – 151

5

Beta strand

157 – 162

6

Helix

163 – 172

10

Beta strand

173 – 175

3

Helix

179 – 186

8

Turn

187 – 189

3

Beta strand

194 – 197

4

Helix

198 – 204

7

Sequences

	Sequence		Length	Mass (Da)
	P14649 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: 6F3DFAC6CCA9877E	FASTA	208	22,764
	10 20 30 40 50 60 MPPKKDVPVK KPAGPSISKP AAKPAAAGAP PAKTKAEPAV PQAPQKTQEP PVDLSKVVIE 70 80 90 100 110 120 FNKDQLEEFK EAFELFDRVG DGKILYSQCG DVMRALGQNP TNAEVLKVLG NPKSDELKSR 130 140 150 160 170 180 RVDFETFLPM LQAVAKNRGQ GTYEDYLEGF RVFDKEGNGK VMGAELRHVL TTLGEKMTEE 190 200 EVETVLAGHE DSNGCINYEA FLKHILSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of human myosin light chains 1sa and 3nm: implications for isoform evolution and function." Hailstones D.L., Gunning P.W. Mol. Cell. Biol. 10:1095-1104(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"A novel isoform of myosin alkali light chain isolated from human muscle cells." Zimmermann K., Starzinski-Powitz A. Nucleic Acids Res. 17:10496-10496(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain and Ovary.
[4]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X16434 mRNA. Translation: CAA34457.1.
M31211 mRNA. Translation: AAA36320.1.
BC012425 mRNA. Translation: AAH12425.1.
BC014400 mRNA. Translation: AAH14400.1.

IPI

IPI00027255.

PIR

MOHUSA. A34757.

RefSeq

NP_001186558.1. NM_001199629.1.
NP_002466.1. NM_002475.4.

UniGene

Hs.632731.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OE9	X-ray	2.05	B	59-208	[»]
1W7I	X-ray	3.00	B	59-208	[»]
1W7J	X-ray	2.00	B	59-208	[»]

ProteinModelPortal

P14649.

ModBase

Search...

Protein-protein interaction databases

IntAct

P14649. 4 interactions.

MINT

MINT-365219.

STRING

9606.ENSP00000207437.

PTM databases

PhosphoSite

P14649.

Polymorphism databases

DMDM

127153.

Proteomic databases

PaxDb

P14649.

PRIDE

P14649.

Protocols and materials databases

DNASU

140465.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000207437; ENSP00000207437; ENSG00000196465.
ENST00000550443; ENSP00000446643; ENSG00000196465.
ENST00000553066; ENSP00000450385; ENSG00000196465.

GeneID

140465.

KEGG

hsa:140465.

UCSC

uc001sjs.3. human.

Organism-specific databases

CTD

140465.

GeneCards

GC12P056546.

HGNC

HGNC:29823. MYL6B.

HPA

HPA038887.
HPA046859.

MIM

609930. gene.

neXtProt

NX_P14649.

PharmGKB

PA144596407.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG5126.

HOGENOM

HOG000233018.

HOVERGEN

HBG012180.

InParanoid

P14649.

KO

K12751.

OMA

FVRMVMS.

OrthoDB

EOG4JM7R0.

Enzyme and pathway databases

Reactome

REACT_17044. Muscle contraction.

Gene expression databases

ArrayExpress

P14649.

Bgee

P14649.

CleanEx

HS_MYL6B.

Genevestigator

P14649.

GermOnline

ENSG00000196465. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 2 hits.

InterPro

IPR011992. EF-hand-like_dom.
IPR002048. EF_hand_dom.
[Graphical view]

Pfam

PF13405. EF_hand_4. 2 hits.
[Graphical view]

SMART

SM00054. EFh. 2 hits.
[Graphical view]

PROSITE

PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14649.

GenomeRNAi

140465.

NextBio

84133.

SOURCE

Search...

Entry information

Entry name

MYL6B_HUMAN

Accession

Primary (citable) accession number: P14649

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	May 1, 2013
This is version 120 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.