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P14644 (PDE4C_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase 4C
EC=3.1.4.17
Alternative name(s):
DPDE1
Gene names
Name:Pde4c
Synonyms:Dpde1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length536 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes By similarity.

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulation

Inhibited by rolipram.

Pathway

Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.

Subunit structure

Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2 By similarity.

Subcellular location

Cell projection › cilium By similarity.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 536›536cAMP-specific 3',5'-cyclic phosphodiesterase 4C
PRO_0000198813

Regions

Nucleotide binding254 – 2585cAMP By similarity
Compositional bias524 – 53411Poly-Glu

Sites

Active site2541Proton donor By similarity
Metal binding2581Divalent metal cation 1 By similarity
Metal binding2941Divalent metal cation 1 By similarity
Metal binding2951Divalent metal cation 1 By similarity
Metal binding2951Divalent metal cation 2 By similarity
Metal binding4121Divalent metal cation 1 By similarity
Binding site2951cAMP By similarity
Binding site4121cAMP By similarity
Binding site4631cAMP By similarity
Site4151Binds AMP, but not cAMP By similarity

Experimental info

Sequence conflict2181R → S in AAA41847. Ref.2
Sequence conflict5071S → N in AAA41847. Ref.2
Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P14644 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 87D12BE2C46642F3

FASTA53660,064
        10         20         30         40         50         60 
NSSRTSSAAS DLHGEDMIVT PFAQVLASLR TVRSNVAALA HGAGSATRQA LLGTPPQSSQ 

        70         80         90        100        110        120 
QAAPAEESGL QLAQETLEEL DWCLEQLETL QTRRSVGEMA SNKFKRMLNR ELTHLSETSR 

       130        140        150        160        170        180 
SGNQVSEYIS QTFLDQQAEV ELPAPPTEDH PWPMAQITGL RKSCHTSLPT AAIPRFGVQT 

       190        200        210        220        230        240 
DQEEQLAKEL EDTNKWGLDV FKVAELSGNR PLTAVIFRVL QERDLLKTFQ IPADTLLRYL 

       250        260        270        280        290        300 
LTLEGHYHSN VAYHNSIHAA DVVQSAHVLL GTPALEAVFT DLEVLAAIFA CAIHDVDHPG 

       310        320        330        340        350        360 
VSNQFLINTN SELALMYNDS SVLENHHLAV GFKLLQGENC DIFQNLSTKQ KLSLRRMVID 

       370        380        390        400        410        420 
MVLATDMSKH MSLLADLKTM VETKKVTSLG VLLLDNYSDR IQVLQSLVHC ADLSNPAKPL 

       430        440        450        460        470        480 
PLYRQWTERI MAEFFQQGDR ERESGLDISP MCDKHTASVE KSQVGFIDYI AHPLWETWAD 

       490        500        510        520        530 
LVHPDAQELL DTLEDNREWY QSRVPCSPPH AIGPDRFKFE LTLEETEEEE EEDERH

« Hide

References

[1]"Differential CNS expression of alternative mRNA isoforms of the mammalian genes encoding cAMP-specific phosphodiesterases."
Bolger G.B., Rodgers L., Riggs M.
Gene 149:237-244(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Molecular cloning of rat homologues of the Drosophila melanogaster dunce cAMP phosphodiesterase: evidence for a family of genes."
Swinnen J.V., Joseph D.R., Conti M.
Proc. Natl. Acad. Sci. U.S.A. 86:5325-5329(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 153-511.
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L27061 mRNA. Translation: AAA56858.1.
M25347 mRNA. Translation: AAA41847.1.
IPIIPI00958858.
PIRI67945.
UniGeneRn.214181.
Rn.92465.

3D structure databases

ProteinModelPortalP14644.
SMRP14644. Positions 173-505.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000026457.

Proteomic databases

PRIDEP14644.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

RGD727918. Pde4c.

Phylogenomic databases

eggNOGNOG122287.
HOGENOMHOG000236297.
HOVERGENHBG108239.
InParanoidP14644.
OrthoDBEOG4ZGPBT.

Enzyme and pathway databases

UniPathwayUPA00762; UER00747.

Gene expression databases

GenevestigatorP14644.
GermOnlineENSRNOG00000019518. Rattus norvegicus.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP14644.
ChEMBLCHEMBL4562.

Entry information

Entry namePDE4C_RAT
AccessionPrimary (citable) accession number: P14644
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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