Reviewed, UniProtKB/Swiss-Prot P14644 (PDE4C_RAT)

Names and origin

Protein names

Recommended name:
    cAMP-specific 3',5'-cyclic phosphodiesterase 4C
    EC=3.1.4.17
Alternative name(s):
    DPDE1

Gene names

Name:	Pde4c
Synonyms:	Dpde1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	536 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Enzyme regulation	Inhibited by rolipram.
Pathway	Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family.

Ontologies

Keywords
Ligand	cAMP
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cAMP catabolic process Inferred from mutant phenotype. Source: RGD negative regulation of insulin secretion Inferred from mutant phenotype. Source: RGD signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from mutant phenotype. Source: RGD drug binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 536	›536	cAMP-specific 3',5'-cyclic phosphodiesterase 4C		PRO_0000198813
Regions
	Compositional bias	524 – 534	11	Poly-Glu
Amino acid modifications
	Modified residue	31	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	218	1	R → S in AAA41847. Ref.2
	Sequence conflict	507	1	S → N in AAA41847. Ref.2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P14644-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 87D12BE2C46642F3	FASTA	536	60,064
	10 20 30 40 50 60 NSSRTSSAAS DLHGEDMIVT PFAQVLASLR TVRSNVAALA HGAGSATRQA LLGTPPQSSQ 70 80 90 100 110 120 QAAPAEESGL QLAQETLEEL DWCLEQLETL QTRRSVGEMA SNKFKRMLNR ELTHLSETSR 130 140 150 160 170 180 SGNQVSEYIS QTFLDQQAEV ELPAPPTEDH PWPMAQITGL RKSCHTSLPT AAIPRFGVQT 190 200 210 220 230 240 DQEEQLAKEL EDTNKWGLDV FKVAELSGNR PLTAVIFRVL QERDLLKTFQ IPADTLLRYL 250 260 270 280 290 300 LTLEGHYHSN VAYHNSIHAA DVVQSAHVLL GTPALEAVFT DLEVLAAIFA CAIHDVDHPG 310 320 330 340 350 360 VSNQFLINTN SELALMYNDS SVLENHHLAV GFKLLQGENC DIFQNLSTKQ KLSLRRMVID 370 380 390 400 410 420 MVLATDMSKH MSLLADLKTM VETKKVTSLG VLLLDNYSDR IQVLQSLVHC ADLSNPAKPL 430 440 450 460 470 480 PLYRQWTERI MAEFFQQGDR ERESGLDISP MCDKHTASVE KSQVGFIDYI AHPLWETWAD 490 500 510 520 530 LVHPDAQELL DTLEDNREWY QSRVPCSPPH AIGPDRFKFE LTLEETEEEE EEDERH

References

[1]	"Differential CNS expression of alternative mRNA isoforms of the mammalian genes encoding cAMP-specific phosphodiesterases." Bolger G.B., Rodgers L., Riggs M. Gene 149:237-244(1994) [PubMed: 7958996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning of rat homologues of the Drosophila melanogaster dunce cAMP phosphodiesterase: evidence for a family of genes." Swinnen J.V., Joseph D.R., Conti M. Proc. Natl. Acad. Sci. U.S.A. 86:5325-5329(1989) [PubMed: 2546153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 153-511. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27061 mRNA. Translation: AAA56858.1. M25347 mRNA. Translation: AAA41847.1.
IPI	IPI00362634.
PIR	I67945.
UniGene	Rn.214181 Rn.92465
3D structure databases
HSSP	HSSP built from PDB template 1OYN based on UniProtKB Q08499.
SMR	P14644. Positions 173-505.
ModBase	Search...
Protein-protein interaction databases
STRING	P14644.
Genome annotation databases
Ensembl	ENSRNOT00000026457; ENSRNOP00000026457; ENSRNOG00000019518; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	727918. Pde4c.
Phylogenomic databases
HOVERGEN	P14644.
Enzyme and pathway databases
BRENDA	3.1.4.17. 248.
Gene expression databases
ArrayExpress	P14644.
Genevestigator	P14644.
GermOnline	ENSRNOG00000019518. Rattus norvegicus.
Family and domain databases
InterPro	IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE4C_RAT

Accession

Primary (citable) accession number: P14644

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)