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Protein

cAMP-specific 3',5'-cyclic phosphodiesterase 4C

Gene

Pde4c

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.By similarity

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by rolipram.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei254 – 2541Proton donorBy similarity
Metal bindingi258 – 2581Divalent metal cation 1By similarity
Metal bindingi294 – 2941Divalent metal cation 1By similarity
Metal bindingi295 – 2951Divalent metal cation 1By similarity
Metal bindingi295 – 2951Divalent metal cation 2By similarity
Binding sitei295 – 2951cAMPBy similarity
Metal bindingi412 – 4121Divalent metal cation 1By similarity
Binding sitei412 – 4121cAMPBy similarity
Sitei415 – 4151Binds AMP, but not cAMPBy similarity
Binding sitei463 – 4631cAMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi254 – 2585cAMPBy similarity

GO - Molecular functioni

  1. 3',5'-cyclic-AMP phosphodiesterase activity Source: RGD
  2. drug binding Source: RGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cAMP catabolic process Source: UniProtKB
  2. negative regulation of insulin secretion Source: RGD
  3. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cAMP, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-specific 3',5'-cyclic phosphodiesterase 4C (EC:3.1.4.53)
Alternative name(s):
DPDE1
Gene namesi
Name:Pde4c
Synonyms:Dpde1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi727918. Pde4c.

Subcellular locationi

  1. Cell projectioncilium By similarity

GO - Cellular componenti

  1. primary cilium Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 536›536cAMP-specific 3',5'-cyclic phosphodiesterase 4CPRO_0000198813Add
BLAST

Proteomic databases

PRIDEiP14644.

Expressioni

Gene expression databases

GenevestigatoriP14644.

Interactioni

Subunit structurei

Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026457.

Structurei

3D structure databases

ProteinModelPortaliP14644.
SMRiP14644. Positions 173-505.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi524 – 53411Poly-GluAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG122287.
HOGENOMiHOG000236297.
HOVERGENiHBG108239.
InParanoidiP14644.
PhylomeDBiP14644.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P14644-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
NSSRTSSAAS DLHGEDMIVT PFAQVLASLR TVRSNVAALA HGAGSATRQA
60 70 80 90 100
LLGTPPQSSQ QAAPAEESGL QLAQETLEEL DWCLEQLETL QTRRSVGEMA
110 120 130 140 150
SNKFKRMLNR ELTHLSETSR SGNQVSEYIS QTFLDQQAEV ELPAPPTEDH
160 170 180 190 200
PWPMAQITGL RKSCHTSLPT AAIPRFGVQT DQEEQLAKEL EDTNKWGLDV
210 220 230 240 250
FKVAELSGNR PLTAVIFRVL QERDLLKTFQ IPADTLLRYL LTLEGHYHSN
260 270 280 290 300
VAYHNSIHAA DVVQSAHVLL GTPALEAVFT DLEVLAAIFA CAIHDVDHPG
310 320 330 340 350
VSNQFLINTN SELALMYNDS SVLENHHLAV GFKLLQGENC DIFQNLSTKQ
360 370 380 390 400
KLSLRRMVID MVLATDMSKH MSLLADLKTM VETKKVTSLG VLLLDNYSDR
410 420 430 440 450
IQVLQSLVHC ADLSNPAKPL PLYRQWTERI MAEFFQQGDR ERESGLDISP
460 470 480 490 500
MCDKHTASVE KSQVGFIDYI AHPLWETWAD LVHPDAQELL DTLEDNREWY
510 520 530
QSRVPCSPPH AIGPDRFKFE LTLEETEEEE EEDERH
Length:536
Mass (Da):60,064
Last modified:October 1, 1996 - v2
Checksum:i87D12BE2C46642F3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti218 – 2181R → S in AAA41847 (PubMed:2546153).Curated
Sequence conflicti507 – 5071S → N in AAA41847 (PubMed:2546153).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27061 mRNA. Translation: AAA56858.1.
M25347 mRNA. Translation: AAA41847.1.
PIRiI67945.
UniGeneiRn.214181.
Rn.92465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27061 mRNA. Translation: AAA56858.1.
M25347 mRNA. Translation: AAA41847.1.
PIRiI67945.
UniGeneiRn.214181.
Rn.92465.

3D structure databases

ProteinModelPortaliP14644.
SMRiP14644. Positions 173-505.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026457.

Chemistry

ChEMBLiCHEMBL2094267.

Proteomic databases

PRIDEiP14644.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

RGDi727918. Pde4c.

Phylogenomic databases

eggNOGiNOG122287.
HOGENOMiHOG000236297.
HOVERGENiHBG108239.
InParanoidiP14644.
PhylomeDBiP14644.

Enzyme and pathway databases

UniPathwayiUPA00762; UER00747.

Gene expression databases

GenevestigatoriP14644.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Differential CNS expression of alternative mRNA isoforms of the mammalian genes encoding cAMP-specific phosphodiesterases."
    Bolger G.B., Rodgers L., Riggs M.
    Gene 149:237-244(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular cloning of rat homologues of the Drosophila melanogaster dunce cAMP phosphodiesterase: evidence for a family of genes."
    Swinnen J.V., Joseph D.R., Conti M.
    Proc. Natl. Acad. Sci. U.S.A. 86:5325-5329(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 153-511.
    Tissue: Testis.

Entry informationi

Entry nameiPDE4C_RAT
AccessioniPrimary (citable) accession number: P14644
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.