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Protein

Glutamine synthetase nodule isozyme

Gene
N/A
Organism
Lupinus angustifolius (Narrow-leaved blue lupine)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-ammonia ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine biosynthetic process Source: InterPro
  2. nitrogen fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase nodule isozyme (EC:6.3.1.2)
Alternative name(s):
Glutamate--ammonia ligase
OrganismiLupinus angustifolius (Narrow-leaved blue lupine)
Taxonomic identifieri3871 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeGenisteaeLupinus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›315›315Glutamine synthetase nodule isozymePRO_0000153176Add
BLAST

Interactioni

Subunit structurei

Homooctamer.

Structurei

3D structure databases

ProteinModelPortaliP14636.
SMRiP14636. Positions 4-315.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

P14636-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLSDLINL NLSDTTEKII AEYIWVGGSG VDLRSKARTL SGPVNDPSKL
60 70 80 90 100
PKWNYDGSST GQAPGKDSEV ILWPQAIFKD PFRRGNNILV ICDTYTPSGK
110 120 130 140 150
PIPTNKRHAA AKIFSHPDVA AEEPWFGIEQ EYTLLQKDIH WPIGMALGGF
160 170 180 190 200
PGPQGPYYCG TGAEKAFGRD IVDSHYKACL YAGINISGIN AEVMPGQWEF
210 220 230 240 250
QVGPSIGISA GDELWVARYI LERITEIAGV VLSLDPKPIP GDWNGAGAHT
260 270 280 290 300
NYSTKSMRND GGYEVIKQAI EKLEKRHNEH IAAYGEGNER RLTGRHETAD
310
ISTFSWGVAN RGASI
Length:315
Mass (Da):34,441
Last modified:April 1, 1990 - v1
Checksum:iDC3B61A903F442F3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei315 – 3151

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15578 mRNA. Translation: CAA33605.1.
PIRiS07462. AJYLNB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15578 mRNA. Translation: CAA33605.1.
PIRiS07462. AJYLNB.

3D structure databases

ProteinModelPortaliP14636.
SMRiP14636. Positions 4-315.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The isolation and characterization of a cDNA clone encoding Lupinus angustifolius root nodule glutamine synthetase."
    Grant M.R., Carne A., Hill D.F., Farnden K.J.F.
    Plant Mol. Biol. 13:481-490(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Uniharvest.
    Tissue: Root nodule.

Entry informationi

Entry nameiGLNA3_LUPAN
AccessioniPrimary (citable) accession number: P14636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: April 1, 1990
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.