P14626 (RNASL_RANCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribonuclease, liver EC=3.1.27.5
Organism	Rana catesbeiana (American bullfrog) (Lithobates catesbeiana)
Taxonomic identifier	8400 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Raninae › Rana › Aquarana

Protein attributes

Sequence length	111 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subcellular location	Secreted.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 111	111	Ribonuclease, liver		PRO_0000057172
Regions
	Region	35 – 39	5	Substrate binding By similarity
Sites
	Active site	10	1	Proton acceptor By similarity
	Active site	104	1	Proton donor By similarity
	Binding site	5	1	Substrate By similarity
	Binding site	60	1	Substrate By similarity
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid Ref.1
	Disulfide bond	19 ↔ 72		By similarity
	Disulfide bond	34 ↔ 82		By similarity
	Disulfide bond	52 ↔ 97		By similarity
	Disulfide bond	94 ↔ 111		Probable

Sequences

	Sequence		Length	Mass (Da)
	P14626 [UniParc]. Last modified February 1, 1994. Version 2. Checksum: D64BA72456C10788	FASTA	111	12,461
	10 20 30 40 50 60 QNWAKFKEKH IRSTSSIDCN TIMDKAIYIV GGKCKERNTF IISSEDNVKA ICSGVSPDRK 70 80 90 100 110 ELSTTSFKLN TCIRDSITPR PCPYHPSPDN NKICVKCEKQ LPVHFVGIGK C

References

[1]	"Primary structure of a ribonuclease from bullfrog (Rana catesbeiana) liver." Nitta R., Katayama N., Okabe Y., Iwama M., Watanabe H., Abe Y., Okazaki T., Ohgi K., Irie M. J. Biochem. 106:729-735(1989) [PubMed: 2613682] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
PIR	JX0085.
3D structure databases
ProteinModelPortal	P14626.
SMR	P14626. Positions 1-111.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Enzyme and pathway databases
BRENDA	3.1.27.5. 5278.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNASL_RANCA

Accession

Primary (citable) accession number: P14626

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	February 1, 1994
Last modified:	May 31, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program