Reviewed, UniProtKB/Swiss-Prot P14619 (KGP1B_HUMAN)

Names and origin

Protein names

Recommended name:
    cGMP-dependent protein kinase 1, beta isozyme
      Short name=cGK 1 beta
      Short name=cGKI-beta
    EC=2.7.11.12

Gene names

Name:	PRKG1
Synonyms:	PRKG1B, PRKGR1B

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Binding of cGMP results in enzyme activation.
Subunit structure	Antiparallel homodimer. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1 By similarity. Interacts with MRVI1.
Miscellaneous	Exhibit a substrate specificity similar but not identical to that of CAK.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 2 cyclic nucleotide-binding domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	ATP-binding Nucleotide-binding cGMP cGMP-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Traceable author statement. Source: ProtInc protein amino acid phosphorylation Traceable author statement. Source: ProtInc signal transduction Ref.1 Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CGK1-beta (identifier: P14619-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CGK1-alpha (identifier: Q13976-1)

The sequence of this isoform can be found in the external entry Q13976-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 686	686	cGMP-dependent protein kinase 1, beta isozyme		PRO_0000086118
Regions
	Domain	375 – 634	260	Protein kinase
	Domain	635 – 686	52	AGC-kinase C-terminal
	Nucleotide binding	118 – 235	118	cGMP 1 By similarity
	Nucleotide binding	236 – 427	192	cGMP 2 By similarity
	Nucleotide binding	381 – 389	9	ATP By similarity
Sites
	Active site	499	1	Proton acceptor By similarity
	Binding site	405	1	ATP By similarity
Natural variations
	Natural variant	264	1	I → V		VAR_046773
	Natural variant	282	1	N → S: dbSNP rs34997494. Ref.7		VAR_046774

Sequences

	Sequence		Length	Mass (Da)
	Isoform CGK1-beta [UniParc]. Last modified April 1, 1990. Version 1. Checksum: A097041AB284F1E6	FASTA	686	77,804
	10 20 30 40 50 60 MGTLRDLQYA LQEKIEELRQ RDALIDELEL ELDQKDELIQ KLQNELDKYR SVIRPATQQA 70 80 90 100 110 120 QKQSASTLQG EPRTKRQAIS AEPTAFDIQD LSHVTLPFYP KSPQSKDLIK EAILDNDFMK 130 140 150 160 170 180 NLELSQIQEI VDCMYPVEYG KDSCIIKEGD VGSLVYVMED GKVEVTKEGV KLCTMGPGKV 190 200 210 220 230 240 FGELAILYNC TRTATVKTLV NVKLWAIDRQ CFQTIMMRTG LIKHTEYMEF LKSVPTFQSL 250 260 270 280 290 300 PEEILSKLAD VLEETHYENG EYIIRQGARG DTFFIISKGT VNVTREDSPS EDPVFLRTLG 310 320 330 340 350 360 KGDWFGEKAL QGEDVRTANV IAAEAVTCLV IDRDSFKHLI GGLDDVSNKA YEDAEAKAKY 370 380 390 400 410 420 EAEAAFFANL KLSDFNIIDT LGVGGFGRVE LVQLKSEESK TFAMKILKKR HIVDTRQQEH 430 440 450 460 470 480 IRSEKQIMQG AHSDFIVRLY RTFKDSKYLY MLMEACLGGE LWTILRDRGS FEDSTTRFYT 490 500 510 520 530 540 ACVVEAFAYL HSKGIIYRDL KPENLILDHR GYAKLVDFGF AKKIGFGKKT WTFCGTPEYV 550 560 570 580 590 600 APEIILNKGH DISADYWSLG ILMYELLTGS PPFSGPDPMK TYNIILRGID MIEFPKKIAK 610 620 630 640 650 660 NAANLIKKLC RDNPSERLGN LKNGVKDIQK HKWFEGFNWE GLRKGTLTPP IIPSVASPTD 670 680 TSNFDSFPED NDEPPPDDNS GWDIDF
	Isoform CGK1-alpha. See Q13976.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and predicted full-length amino acid sequence of the type I beta isozyme of cGMP-dependent protein kinase from human placenta. Tissue distribution and developmental changes in rat." Sandberg M., Natarajan V., Ronander I., Kalderon D., Walter U., Lohmann S.M., Jahnsen T. FEBS Lett. 255:321-329(1989) [PubMed: 2792381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	Sandberg M. Submitted (OCT-1989) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Characterization of the human gene encoding the type I alpha and type I beta cGMP-dependent protein kinase (PRKG1)." Orstavik S., Natarajan V., Tasken K., Jahnsen T., Sandberg M. Genomics 42:311-318(1997) [PubMed: 9192852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"IRAG mediates NO/cGMP-dependent inhibition of platelet aggregation and thrombus formation." Antl M., von Bruehl M.-L., Eiglsperger C., Werner M., Konrad I., Kocher T., Wilm M., Hofmann F., Massberg S., Schlossmann J. Blood 109:552-559(2007) [PubMed: 16990611] [Abstract] Cited for: INTERACTION WITH MRVI1.
[7]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] VAL-264 AND SER-282.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y07512 mRNA. Translation: CAA68810.1. Z92868 , Z92869, Z92870, Z92871, Z92872, Z92873, Z92874, Z92875, Z92876, Z92877, Z92878, Z92879, Z92880, Z92881, Z92882, Z92883, Z92884, Z92885 Genomic DNA. Translation: CAB07437.1. AL391378 , AC009986, AC022537, AC022025, AC027118, AL731537, AL928686, AL157399, AC026228 Genomic DNA. Translation: CAI39626.1. AL928686 , AC009986, AC022537, AL731537, AL391378, AL157399, AC027118, AC026228, AC022025 Genomic DNA. Translation: CAI40743.1. AL157399 , AC022025, AC009986, AL928686, AL731537, AL391378, AC027118, AC026228, AC022537 Genomic DNA. Translation: CAI41305.1. AL731537 , AL928686, AL391378, AL157399, AC027118, AC026228, AC022537, AC022025, AC009986 Genomic DNA. Translation: CAI17115.1. BC127090 mRNA. Translation: AAI27091.1.
IPI	IPI00436355.
PIR	S05702.
RefSeq	NP_001091982.1. NP_006249.1.
UniGene	Hs.654556
3D structure databases
HSSP	HSSP built from PDB template 1ATP based on UniProtKB P05132.
ModBase	Search...
Protein-protein interaction databases
STRING	P14619.
Proteomic databases
PRIDE	P14619.
Genome annotation databases
Ensembl	ENST00000332193; ENSP00000327642; ENSG00000185532; Homo sapiens. [Genome view] ENST00000373975; ENSP00000363086; ENSG00000185532; Homo sapiens. [Genome view] ENST00000373976; ENSP00000363087; ENSG00000185532; Homo sapiens. [Genome view] ENST00000373980; ENSP00000363092; ENSG00000185532; Homo sapiens. [Genome view] ENST00000373985; ENSP00000363097; ENSG00000185532; Homo sapiens. [Genome view] ENST00000401604; ENSP00000384200; ENSG00000185532; Homo sapiens. [Genome view] ENST00000444650; ENSP00000416244; ENSG00000185532; Homo sapiens. [Genome view]
GeneID	5592.
KEGG	hsa:5592.
UCSC	uc001jjo.2. human.
Organism-specific databases
CTD	5592.
GeneCards	GC10P052421.
HGNC	HGNC:9414. PRKG1.
HPA	CAB009629. HPA007699.
MIM	176894. gene.
PharmGKB	PA33777.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P14619.
HOVERGEN	P14619.
OMA	DPIFLRT.
Enzyme and pathway databases
BRENDA	2.7.11.12. 247.
Pathway_Interaction_DB	p38alphabetapathway. Regulation of p38-alpha and p38-beta. txa2pathway. Thromboxane A2 receptor signaling.
Gene expression databases
ArrayExpress	P14619.
Bgee	P14619.
CleanEx	HS_PRKG1.
Genevestigator	P14619.
GermOnline	ENSG00000185532. Homo sapiens.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR016232. cGMP-dependent_protein_kinase. IPR002374. cGMP_kinase_core. IPR000595. cNMP_bd. IPR018488. cNMP_bd_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR014710. RmlC-like_jellyroll. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits.
Pfam	PF00027. cNMP_binding. 2 hits. PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000559. cGMP-dep_kinase. 1 hit.
PRINTS	PR00104. CGMPKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00100. cNMP. 2 hits. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	21698.
SOURCE	Search...

Entry information

Entry name

KGP1B_HUMAN

Accession

Primary (citable) accession number: P14619
Secondary accession number(s): Q5JSJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	November 3, 2009
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.