P14605 (CYAA_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate cyclase
EC=4.6.1.1

Alternative name(s):
ATP pyrophosphate-lyase
Adenylyl cyclase

Gene names

Name:	cyr1
ORF Names:	SPBC19C7.03

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1692 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays essential roles in regulation of cellular metabolism by catalyzing the synthesis of a second messenger, cAMP.
Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Enzyme regulation	In contrast to yeast cyclase, S.pombe cyclase is not likely to be regulated by RAS proteins. Activated by git1.
Subunit structure	Interacts with git1. Ref.4
Subcellular location	Cytoplasm Ref.4.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain. Contains 21 LRR (leucine-rich) repeats. Contains 1 PP2C-like domain. Contains 1 Ras-associating domain.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Cellular component	Cytoplasm
Domain	Leucine-rich repeat Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	G-protein signaling, coupled to cAMP nucleotide second messenger Non-traceable author statement. Source: GeneDB_Spombe negative regulation of transcription by glucose Traceable author statement. Source: GeneDB_Spombe regulation of conjugation with cellular fusion Non-traceable author statement. Source: GeneDB_Spombe
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Non-traceable author statement. Source: GeneDB_Spombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from mutant phenotype. Source: GeneDB_Spombe magnesium ion binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.4. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
git1	Q9P7K5	3	EBI-1542835,EBI-1542810

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1692	1692	Adenylate cyclase		PRO_0000195733
Regions
	Domain	292 – 380	89	Ras-associating
	Repeat	430 – 450	21	LRR 1
	Repeat	454 – 474	21	LRR 2
	Repeat	477 – 498	22	LRR 3
	Repeat	503 – 524	22	LRR 4
	Repeat	526 – 547	22	LRR 5
	Repeat	549 – 570	22	LRR 6
	Repeat	572 – 594	23	LRR 7
	Repeat	596 – 617	22	LRR 8
	Repeat	618 – 639	22	LRR 9
	Repeat	660 – 681	22	LRR 10
	Repeat	684 – 705	22	LRR 11
	Repeat	707 – 729	23	LRR 12
	Repeat	730 – 751	22	LRR 13
	Repeat	753 – 774	22	LRR 14
	Repeat	783 – 805	23	LRR 15
	Repeat	807 – 827	21	LRR 16
	Repeat	831 – 852	22	LRR 17
	Repeat	855 – 876	22	LRR 18
	Repeat	878 – 899	22	LRR 19
	Repeat	901 – 922	22	LRR 20
	Repeat	930 – 951	22	LRR 21
	Domain	1008 – 1276	269	PP2C-like
	Domain	1332 – 1469	138	Guanylate cyclase
Sites
	Metal binding	1337	1	Magnesium By similarity
	Metal binding	1380	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14605 [UniParc]. Last modified April 1, 1990. Version 1. Checksum: D137CBE8770A8655	FASTA	1,692	190,334
	10 20 30 40 50 60 MDQSKRLLKS AVPNPPEHFK TGISWLDDLD EKDDDSATSV NYDIPEITEA NLCNDSHEAL 70 80 90 100 110 120 SPCTQPVGNS GRPVEAFKTY PSTPAVPSKS VLFHFYEPDE NFSLSDTGRT KSDTALAARE 130 140 150 160 170 180 SSEKSEVPRD TRSAGIKPYK ENNSSNCAIS KEAGLRRLID KDRESFDKNL NQSFTNLTFP 190 200 210 220 230 240 EPISDDSDSV EFQRDSLNNN WPASLEGSIH ELPRNSDDDG IPASAAHILD LDYHRDSYDS 250 260 270 280 290 300 PWKKFLPYPS ILSDDSWKAP ESWGTSLPTE AIPKQVFTTR FFARPSLGNR KKEFFLRVYR 310 320 330 340 350 360 DDRTSVSFIC PIGIQTHEVI KLLARLFFLP SSANFYLLLI QFNTERILLP HEQPCIIFER 370 380 390 400 410 420 LLSLFGCKVT SDEEINEEDN YSVARLVFTT MDIGADVLRK FSEKKITANL DISRSNLEVI 430 440 450 460 470 480 PVKIYPYAHE LISLNVSHNL SLDLPLDFME RCVKLKRLDI SNNLRSPRGK PITALRQLEV 490 500 510 520 530 540 LNMSRNDIYE LDPLIFSGLS RNSLKELNIA NNKLFFLPHS TRYLVNLTYL DLSYNNFVTF 550 560 570 580 590 600 PLIITELSQL ETLNFSHNLL SQISSKIGSL VKLKHLYLQF NDLSNRLPQE IGLLKNLETI 610 620 630 640 650 660 DLSYNAITNI ASLSECPKLN SINVACNLLS FYEYSNPSAT FIDFSFCPLT TIDPAFSYSN 670 680 690 700 710 720 LVYFDISHAK LIGLKDSVIE TLVNVETVKV NYNHFTSISD AISAMQNLKY LSCTNCEMSY 730 740 750 760 770 780 VSPNLGKLKH LVHLDLHANN IKIFPEEVWQ VSSLKVVNLS SNILEKIKLP VATSKKLTRT 790 800 810 820 830 840 ISQLKIMRTL SGNPVSSLSS QEFVMPTVEE LYLVDNRLGN DCFTALEYFK CLKVLNLSYN 850 860 870 880 890 900 YLTEIPSKFF QNFSDLKHLF VSGNELANLS ISSTAQVLLE TLYANGNRLS SFPKNEALSK 910 920 930 940 950 960 SLRFLDISTN NLQNLAVEKA EKKSLTKLPQ LEYLNLSGNT WFRFSEHEDT NFTKSYLKNL 970 980 990 1000 1010 1020 KFLSIMDLNT KFSNAPSDVL NHFIQRNSPQ PNILRYGVCG YLSRSIPVIS ACELVVNNFL 1030 1040 1050 1060 1070 1080 HPQSSLYCVL DSDISAGKNN RVLKFVYDNL ASCLAHEINA ADSSSEQICN ALRRGFLRLN 1090 1100 1110 1120 1130 1140 KKLGNVIHYD LRKSSEGDVD SNYVTTMNIS EKGYSMDSSC LDIGVSIILV YVRDTRAFVA 1150 1160 1170 1180 1190 1200 NVGTSMAIMS TRNDSEPTTL SVMHDVYNRD EIRRIVDSCG FISGEIKSTT TRAIGRLSQF 1210 1220 1230 1240 1250 1260 PGVQAVPYVN VQYLSELNEF IILANQEFWS VLSKRTVIDV VRANRHSPLL ASTKLRDYAI 1270 1280 1290 1300 1310 1320 AYGAEKNVLV VIVELNGLFE ENSLNFNQLR GDEKTLAISE KNDNMSFVQD LPDDSSLARM 1330 1340 1350 1360 1370 1380 NREVSPPKGC IAMVFTDIKN STLLWERHPI AMRSAIKTHN TIMRRQLRAT GGYEVKTEGD 1390 1400 1410 1420 1430 1440 AFMVCFQTVP AALLWCFSVQ LQLLSADWPN EIVESVQGRL VLGSKNEVLY RGLSVRIGVN 1450 1460 1470 1480 1490 1500 YGVTVSELDP ITRRMDYYGP VVNRTSRVVS VADGGQIAVS AEVVSVLNQL DSETMSSEKT 1510 1520 1530 1540 1550 1560 NVNEMEVRAL KQIGYIIHNL GEFKLKGLDT TEMISLVYPV QLQGRLERLI KSRSLGTPTA 1570 1580 1590 1600 1610 1620 LPETQTYTPV RSRSNSLRPM LARLSDSKSV HGEEGGSGKR SVSSLRNVSP SESTGGYEGC 1630 1640 1650 1660 1670 1680 IFDDQQYQLL YELCERLEDH AAILHGFPEP PPCDTGLAAP VNQAEEYSLF YRLTLRIENT 1690 IYCVSQMLGH TG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The adenylyl cyclase gene from Schizosaccharomyces pombe." Young D., Riggs M., Field J., Vojtek A., Broek D., Wigler M. Proc. Natl. Acad. Sci. U.S.A. 86:7989-7993(1989) [PubMed: 2682634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Adenylate cyclases in yeast: a comparison of the genes from Schizosaccharomyces pombe and Saccharomyces cerevisiae." Yamawaki-Kataoka Y., Tamaoki T., Choe H.-R., Tanaka H., Kataoka T. Proc. Natl. Acad. Sci. U.S.A. 86:5693-5697(1989) [PubMed: 2668944] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[4]	"Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose activation of adenylate cyclase." Kao R.S., Morreale E., Wang L., Ivey F.D., Hoffman C.S. Genetics 173:49-61(2006) [PubMed: 16489217] [Abstract] Cited for: ACTIVATION BY GIT1, INTERACTION WITH GIT1, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M26699 Genomic DNA. Translation: AAA35284.1. M24942 Genomic DNA. Translation: AAA35301.1. CU329671 Genomic DNA. Translation: CAA19571.1.
PIR	A33988.
RefSeq	NP_596159.1. NM_001022079.1.
3D structure databases
ProteinModelPortal	P14605.
ModBase	Search...
Protein-protein interaction databases
IntAct	P14605. 1 interaction.
STRING	P14605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC19C7.03.1; SPBC19C7.03.1:pep; SPBC19C7.03.
GeneID	2540810.
GenomeReviews	Gene locus cyr1 in contig CU329671_GR.
KEGG	spo:SPBC19C7.03.
NMPDR	fig|4896.1.peg.2025.
Organism-specific databases
GeneDB_Spombe	SPBC19C7.03.
Phylogenomic databases
eggNOG	fuNOG06104.
GeneTree	EFGT00050000002537.
OMA	NSTHLWE.
OrthoDB	EOG41CB4C.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004193-MONOMER.
Gene expression databases
ArrayExpress	P14605.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR013716. Adenylate_cyclase_G-a-bd. IPR001611. Leu-rich_rpt. IPR003591. Leu-rich_rpt_typical-subtyp. IPR001932. PP2C-like. IPR000159. Ras-assoc. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 1 hit. G3DSA:3.60.40.10. PP2C-related. 1 hit.
KO	K01768.
Pfam	PF08509. Ad_cyc_g-alpha. 1 hit. PF00211. Guanylate_cyc. 1 hit. PF00560. LRR_1. 2 hits. PF00481. PP2C. 1 hit. PF00788. RA. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. SM00369. LRR_TYP. 1 hit. SM00332. PP2Cc. 1 hit. SM00314. RA. 1 hit. [Graphical view]
SUPFAM	SSF55073. A/G_cyclase. 1 hit. SSF81606. PP2C-related. 1 hit.
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. PS51450. LRR. 14 hits. PS50200. RA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYAA_SCHPO

Accession

Primary (citable) accession number: P14605

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	April 1, 1990
Last modified:	December 14, 2011
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program