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Reviewed, UniProtKB/Swiss-Prot P14602 (HSPB1_MOUSE)

Last modified July 13, 2010. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
Heat shock protein beta-1

Short name=HspB1
Alternative name(s):
Heat shock 27 kDa protein
Short name=HSP 27
Growth-related 25 kDa protein
Heat shock 25 kDa protein
Short name=HSP 25
p25
Gene names
Name:Hspb1
Synonyms:Hsp25, Hsp27
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus
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Protein attributesHide

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Function

Involved in stress resistance and actin organization.

Subunit structure

Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 By similarity. Interacts with HSPBAP1 and TGFB1I1. Ref.7 Ref.8

Sequence similarities

Belongs to the small heat shock protein (HSP20) family.

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OntologiesHide

Keywords
   Biological processStress response
   Coding sequence diversityAlternative splicing
   PTMAcetylation
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processresponse to heat

Inferred from electronic annotation. Source: InterPro

   Cellular componentZ disc

Inferred from direct assay. Source: MGI

insoluble fraction

Inferred from direct assay. Source: MGI

nucleus

Inferred from direct assay. Source: MGI

plasma membrane

Inferred from direct assay. Source: MGI

soluble fraction

Inferred from direct assay. Source: MGI

Complete GO annotation...
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Alternative productsHide

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform A (identifier: P14602-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: P14602-2)

The sequence of this isoform differs from the canonical sequence as follows:
     61-72: Missing.
Isoform C (identifier: P14602-3)

The sequence of this isoform differs from the canonical sequence as follows:
     37-70: Missing.
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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Heat shock protein beta-1
PRO_0000125928

Regions

Region74 – 209136Interaction with TGFB1I1 By similarity

Amino acid modifications

Modified residue131Phosphoserine By similarity
Modified residue151Phosphoserine; by PKA and PKC Ref.6 Ref.9
Modified residue271Phosphoserine By similarity
Modified residue861Phosphoserine; by PKA and PKC Ref.6
Modified residue871Phosphoserine By similarity
Modified residue1271N6-acetyllysine By similarity
Modified residue2031Phosphoserine By similarity

Natural variations

Alternative sequence37 – 7034Missing in isoform C.
VSP_002423
Alternative sequence61 – 7212Missing in isoform B.
VSP_002422

Experimental info

Sequence conflict131S → T in AAA18335. Ref.4
Sequence conflict131S → T in AAA18336. Ref.4
Sequence conflict131S → T in AAA18337. Ref.4
Sequence conflict171E → G in AAA18335. Ref.4
Sequence conflict67 – 682PA → L in AAA18335. Ref.4
Sequence conflict991Missing in CAA32818. Ref.3
Sequence conflict991Missing in CAB37341. Ref.3
Sequence conflict108 – 1092FA → VI in CAA32818. Ref.3
Sequence conflict108 – 1092FA → VI in CAB37341. Ref.3
Sequence conflict1411C → Q AA sequence Ref.3
Sequence conflict1811A → T in CAA32818. Ref.3
Sequence conflict1811A → T in CAB37341. Ref.3
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SequencesHide

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified June 1, 1994. Version 3.
Checksum: 31BD9FAF4E107C50

FASTA20923,014
        10         20         30         40         50         60 
MTERRVPFSL LRSPSWEPFR DWYPAHSRLF DQAFGVPRLP DEWSQWFSAA GWPGYVRPLP 

        70         80         90        100        110        120 
AATAEGPAAV TLAAPAFSRA LNRQLSSGVS EIRQTADRWR VSLDVNHFAP EELTVKTKEG 

       130        140        150        160        170        180 
VVEITGKHEE RQDEHGYISR CFTRKYTLPP GVDPTLVSSS LSPEGTLTVE APLPKAVTQS 

       190        200 
AEITIPVTFE ARAQIGGPEA GKSEQSGAK 

« Hide

Isoform B.

Checksum: 071D3C112509C01A
Show »

FASTA19721,961
Isoform C.

Checksum: AA7E60872B1C485B
Show »

FASTA17519,380
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ReferencesHide

« Hide 'large scale' references
[1]"Structure and organisation of a murine gene encoding small heat-shock protein Hsp25."
Gaestel M., Gotthardt R., Mueller T.
Gene 128:279-283(1993) [PubMed: 8514194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
[2]"Cloning of the mouse hsp25 gene and an extremely conserved hsp25 pseudogene."
Froehli E., Aoyama X., Klemenz R.
Gene 128:273-277(1993) [PubMed: 8514193] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
[3]"Molecular cloning, sequencing and expression in Escherichia coli of the 25-kDa growth-related protein of Ehrlich ascites tumor and its homology to mammalian stress proteins."
Gaestel M., Gross B., Benndorf R., Strauss M., Schunk W.-H., Kraft R., Otto A., Boehm H., Stahl J., Drabsch H., Bielka H.
Eur. J. Biochem. 179:209-213(1989) [PubMed: 2645135] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), PARTIAL PROTEIN SEQUENCE.
[4]"Differential estrogenic regulation of small M(r) heat shock protein expression in osteoblasts."
Cooper L.F., Uoshima K.
J. Biol. Chem. 269:7869-7873(1994) [PubMed: 8132504] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C).
Tissue: Bone.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
Strain: FVB/N.
Tissue: Mammary gland.
[6]"Identification of the phosphorylation sites of the murine small heat shock protein hsp25."
Gaestel M., Schroeder W., Benndorf R., Lippmann C., Buchner K., Hucho F., Erdmann V.A., Bielka H.
J. Biol. Chem. 266:14721-14724(1991) [PubMed: 1860870] [Abstract]
Cited for: PHOSPHORYLATION AT SER-15 AND SER-86.
[7]"Identification and characterization of a novel protein from Sertoli cells, PASS1, that associates with mammalian small stress protein hsp27."
Liu C., Gilmont R.R., Benndorf R., Welsh M.J.
J. Biol. Chem. 275:18724-18731(2000) [PubMed: 10751411] [Abstract]
Cited for: INTERACTION WITH HSPBAP1.
[8]"Identification and characterization of hic-5/ARA55 as an hsp27 binding protein."
Jia Y., Ransom R.F., Shibanuma M., Liu C., Welsh M.J., Smoyer W.E.
J. Biol. Chem. 276:39911-39918(2001) [PubMed: 11546764] [Abstract]
Cited for: INTERACTION WITH TGFB1I1.
[9]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ
X14687 mRNA. Translation: CAA32818.1.
X14686 mRNA. Translation: CAB37341.1.
L11609, L11608 Genomic DNA. Translation: AAA37862.1.
L07577 Genomic DNA. Translation: AAA37861.1.
U03560 mRNA. Translation: AAA18335.1.
U03561 mRNA. Translation: AAA18336.1.
U03562 mRNA. Translation: AAA18337.1.
BC018257 mRNA. Translation: AAH18257.1.
IPIIPI00128522.
IPI00468068.
IPI00623819.
PIRA53423.
JN0679. I49763.
S02143.
RefSeqNP_038588.2.
UniGeneMm.13849

3D structure databases

SMRP14602. Positions 97-176.
DisProtDP00142.
ModBaseSearch...

Protein-protein interaction databases

IntActP14602. 3 interactions.
STRINGP14602.

PTM databases

PhosphoSiteP14602.

2-D gel databases

SWISS-2DPAGEP14602.
REPRODUCTION-2DPAGEIPI00128522.
IPI00468068.
P14602.
UCD-2DPAGEP14602.

Proteomic databases

PRIDEP14602.

Genome annotation databases

EnsemblENSMUST00000005077; ENSMUSP00000005077; ENSMUSG00000004951; Mus musculus. [Genome view]
ENSMUST00000111156; ENSMUSP00000106786; ENSMUSG00000004951; Mus musculus. [Genome view]
GeneID15507.
KEGGmmu:15507.

Organism-specific databases

CTD15507.
MGIMGI:96240. Hspb1.

Phylogenomic databases

eggNOGroNOG11002.
HOGENOMHBG748242.
HOVERGENHBG054766.
InParanoidP14602.
OMAAEITIPV.
PhylomeDBP14602.

Gene expression databases

ArrayExpressP14602.
BgeeP14602.
CleanExMM_HSPB1.
GenevestigatorP14602.
GermOnlineENSMUSG00000004951. Mus musculus.

Family and domain databases

InterProIPR001436. Alpha-crystallin/HSP.
IPR002068. Hsp20.
IPR008978. HSP20-like_chaperone.
[Graphical view]
PfamPF00011. HSP20. 1 hit.
[Graphical view]
PRINTSPR00299. ACRYSTALLIN.
SUPFAMSSF49764. HSP20_chap. 1 hit.
PROSITEPS01031. HSP20. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio288412.
SOURCESearch...
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Entry informationHide

Entry nameHSPB1_MOUSE
AccessionPrimary (citable) accession number: P14602
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: June 1, 1994
Last modified: July 13, 2010
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
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Relevant documentsHide

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents