Reviewed, UniProtKB/Swiss-Prot P14602 (HSPB1_MOUSE)

Names and originHide

Protein names

Recommended name:
Heat shock protein beta-1
Short name=HspB1

Alternative name(s):
Heat shock 27 kDa protein
Short name=HSP 27
Growth-related 25 kDa protein
Heat shock 25 kDa protein
Short name=HSP 25
p25

Gene names

Name:	Hspb1
Synonyms:	Hsp25, Hsp27

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributesHide

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)Hide

Function	Involved in stress resistance and actin organization.
Subunit structure	Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 By similarity. Interacts with HSPBAP1 and TGFB1I1. Ref.7 Ref.8
Sequence similarities	Belongs to the small heat shock protein (HSP20) family.

OntologiesHide

Keywords
Biological process	Stress response
Coding sequence diversity	Alternative splicing
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to heat Inferred from electronic annotation. Source: InterPro
Cellular component	Z disc Inferred from direct assay. Source: MGI insoluble fraction Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI plasma membrane Inferred from direct assay. Source: MGI soluble fraction Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative productsHide

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform A (identifier: P14602-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P14602-2)

The sequence of this isoform differs from the canonical sequence as follows:
     61-72: Missing.

Isoform C (identifier: P14602-3)

The sequence of this isoform differs from the canonical sequence as follows:
     37-70: Missing.

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 209	209	Heat shock protein beta-1		PRO_0000125928
Regions
	Region	74 – 209	136	Interaction with TGFB1I1 By similarity
Amino acid modifications
	Modified residue	13	1	Phosphoserine By similarity
	Modified residue	15	1	Phosphoserine; by PKA and PKC Ref.6 Ref.9
	Modified residue	27	1	Phosphoserine By similarity
	Modified residue	86	1	Phosphoserine; by PKA and PKC Ref.6
	Modified residue	87	1	Phosphoserine By similarity
	Modified residue	127	1	N6-acetyllysine By similarity
	Modified residue	203	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	37 – 70	34	Missing in isoform C.		VSP_002423
	Alternative sequence	61 – 72	12	Missing in isoform B.		VSP_002422
Experimental info
	Sequence conflict	13	1	S → T in AAA18335. Ref.4
	Sequence conflict	13	1	S → T in AAA18336. Ref.4
	Sequence conflict	13	1	S → T in AAA18337. Ref.4
	Sequence conflict	17	1	E → G in AAA18335. Ref.4
	Sequence conflict	67 – 68	2	PA → L in AAA18335. Ref.4
	Sequence conflict	99	1	Missing in CAA32818. Ref.3
	Sequence conflict	99	1	Missing in CAB37341. Ref.3
	Sequence conflict	108 – 109	2	FA → VI in CAA32818. Ref.3
	Sequence conflict	108 – 109	2	FA → VI in CAB37341. Ref.3
	Sequence conflict	141	1	C → Q AA sequence Ref.3
	Sequence conflict	181	1	A → T in CAA32818. Ref.3
	Sequence conflict	181	1	A → T in CAB37341. Ref.3

SequencesHide

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified June 1, 1994. Version 3. Checksum: 31BD9FAF4E107C50	FASTA	209	23,014
	10 20 30 40 50 60 MTERRVPFSL LRSPSWEPFR DWYPAHSRLF DQAFGVPRLP DEWSQWFSAA GWPGYVRPLP 70 80 90 100 110 120 AATAEGPAAV TLAAPAFSRA LNRQLSSGVS EIRQTADRWR VSLDVNHFAP EELTVKTKEG 130 140 150 160 170 180 VVEITGKHEE RQDEHGYISR CFTRKYTLPP GVDPTLVSSS LSPEGTLTVE APLPKAVTQS 190 200 AEITIPVTFE ARAQIGGPEA GKSEQSGAK
	Isoform B. Checksum: 071D3C112509C01A	FASTA	197	21,961
	10 20 30 40 50 60 MTERRVPFSL LRSPSWEPFR DWYPAHSRLF DQAFGVPRLP DEWSQWFSAA GWPGYVRPLP 70 80 90 100 110 120 AAPAFSRALN RQLSSGVSEI RQTADRWRVS LDVNHFAPEE LTVKTKEGVV EITGKHEERQ 130 140 150 160 170 180 DEHGYISRCF TRKYTLPPGV DPTLVSSSLS PEGTLTVEAP LPKAVTQSAE ITIPVTFEAR 190 AQIGGPEAGK SEQSGAK
	Isoform C. Checksum: AA7E60872B1C485B	FASTA	175	19,380
	10 20 30 40 50 60 MTERRVPFSL LRSPSWEPFR DWYPAHSRLF DQAFGVTLAA PAFSRALNRQ LSSGVSEIRQ 70 80 90 100 110 120 TADRWRVSLD VNHFAPEELT VKTKEGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP 130 140 150 160 170 TLVSSSLSPE GTLTVEAPLP KAVTQSAEIT IPVTFEARAQ IGGPEAGKSE QSGAK

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and organisation of a murine gene encoding small heat-shock protein Hsp25." Gaestel M., Gotthardt R., Mueller T. Gene 128:279-283(1993) [PubMed: 8514194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
[2]	"Cloning of the mouse hsp25 gene and an extremely conserved hsp25 pseudogene." Froehli E., Aoyama X., Klemenz R. Gene 128:273-277(1993) [PubMed: 8514193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
[3]	"Molecular cloning, sequencing and expression in Escherichia coli of the 25-kDa growth-related protein of Ehrlich ascites tumor and its homology to mammalian stress proteins." Gaestel M., Gross B., Benndorf R., Strauss M., Schunk W.-H., Kraft R., Otto A., Boehm H., Stahl J., Drabsch H., Bielka H. Eur. J. Biochem. 179:209-213(1989) [PubMed: 2645135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), PARTIAL PROTEIN SEQUENCE.
[4]	"Differential estrogenic regulation of small M(r) heat shock protein expression in osteoblasts." Cooper L.F., Uoshima K. J. Biol. Chem. 269:7869-7873(1994) [PubMed: 8132504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C). Tissue: Bone.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: FVB/N. Tissue: Mammary gland.
[6]	"Identification of the phosphorylation sites of the murine small heat shock protein hsp25." Gaestel M., Schroeder W., Benndorf R., Lippmann C., Buchner K., Hucho F., Erdmann V.A., Bielka H. J. Biol. Chem. 266:14721-14724(1991) [PubMed: 1860870] [Abstract] Cited for: PHOSPHORYLATION AT SER-15 AND SER-86.
[7]	"Identification and characterization of a novel protein from Sertoli cells, PASS1, that associates with mammalian small stress protein hsp27." Liu C., Gilmont R.R., Benndorf R., Welsh M.J. J. Biol. Chem. 275:18724-18731(2000) [PubMed: 10751411] [Abstract] Cited for: INTERACTION WITH HSPBAP1.
[8]	"Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." Jia Y., Ransom R.F., Shibanuma M., Liu C., Welsh M.J., Smoyer W.E. J. Biol. Chem. 276:39911-39918(2001) [PubMed: 11546764] [Abstract] Cited for: INTERACTION WITH TGFB1I1.
[9]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-15, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	X14687 mRNA. Translation: CAA32818.1. X14686 mRNA. Translation: CAB37341.1. L11609, L11608 Genomic DNA. Translation: AAA37862.1. L07577 Genomic DNA. Translation: AAA37861.1. U03560 mRNA. Translation: AAA18335.1. U03561 mRNA. Translation: AAA18336.1. U03562 mRNA. Translation: AAA18337.1. BC018257 mRNA. Translation: AAH18257.1.
IPI	IPI00128522. IPI00468068. IPI00623819.
PIR	A53423. JN0679. I49763. S02143.
RefSeq	NP_038588.2.
UniGene	Mm.13849
3D structure databases
SMR	P14602. Positions 97-176.
DisProt	DP00142.
ModBase	Search...
Protein-protein interaction databases
IntAct	P14602. 3 interactions.
STRING	P14602.
PTM databases
PhosphoSite	P14602.
2-D gel databases
SWISS-2DPAGE	P14602.
REPRODUCTION-2DPAGE	IPI00128522. IPI00468068. P14602.
UCD-2DPAGE	P14602.
Proteomic databases
PRIDE	P14602.
Genome annotation databases
Ensembl	ENSMUST00000005077; ENSMUSP00000005077; ENSMUSG00000004951; Mus musculus. [Genome view] ENSMUST00000111156; ENSMUSP00000106786; ENSMUSG00000004951; Mus musculus. [Genome view]
GeneID	15507.
KEGG	mmu:15507.
Organism-specific databases
CTD	15507.
MGI	MGI:96240. Hspb1.
Phylogenomic databases
eggNOG	roNOG11002.
HOGENOM	HBG748242.
HOVERGEN	HBG054766.
InParanoid	P14602.
OMA	AEITIPV.
PhylomeDB	P14602.
Gene expression databases
ArrayExpress	P14602.
Bgee	P14602.
CleanEx	MM_HSPB1.
Genevestigator	P14602.
GermOnline	ENSMUSG00000004951. Mus musculus.
Family and domain databases
InterPro	IPR001436. Alpha-crystallin/HSP. IPR002068. Hsp20. IPR008978. HSP20-like_chaperone. [Graphical view]
Pfam	PF00011. HSP20. 1 hit. [Graphical view]
PRINTS	PR00299. ACRYSTALLIN.
SUPFAM	SSF49764. HSP20_chap. 1 hit.
PROSITE	PS01031. HSP20. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	288412.
SOURCE	Search...

Entry informationHide

Entry name

HSPB1_MOUSE

Accession

Primary (citable) accession number: P14602

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1990
Last sequence update:	June 1, 1994
Last modified:	July 13, 2010
This is version 112 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)