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P14602

- HSPB1_MOUSE

UniProt

P14602 - HSPB1_MOUSE

Protein

Heat shock protein beta-1

Gene

Hspb1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 151 (01 Oct 2014)
      Sequence version 3 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Involved in stress resistance and actin organization.

    GO - Molecular functioni

    1. protein kinase C binding Source: BHF-UCL
    2. protein kinase C inhibitor activity Source: BHF-UCL
    3. ubiquitin binding Source: BHF-UCL

    GO - Biological processi

    1. intracellular signal transduction Source: Ensembl
    2. negative regulation of apoptotic signaling pathway Source: MGI
    3. negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: BHF-UCL
    4. negative regulation of protein kinase activity Source: BHF-UCL
    5. negative regulation of protein serine/threonine kinase activity Source: GOC
    6. positive regulation of angiogenesis Source: Ensembl
    7. positive regulation of blood vessel endothelial cell migration Source: Ensembl
    8. positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway Source: Ensembl
    9. positive regulation of interleukin-1 beta production Source: BHF-UCL
    10. positive regulation of tumor necrosis factor biosynthetic process Source: BHF-UCL
    11. regulation of I-kappaB kinase/NF-kappaB signaling Source: BHF-UCL
    12. response to unfolded protein Source: BHF-UCL
    13. response to virus Source: BHF-UCL

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    ReactomeiREACT_198693. AUF1 (hnRNP D0) destabilizes mRNA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat shock protein beta-1
    Short name:
    HspB1
    Alternative name(s):
    Growth-related 25 kDa protein
    Heat shock 25 kDa protein
    Short name:
    HSP 25
    Heat shock 27 kDa protein
    Short name:
    HSP 27
    p25
    Gene namesi
    Name:Hspb1
    Synonyms:Hsp25, Hsp27
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:96240. Hspb1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Cytoplasmcytoskeletonspindle By similarity
    Note: Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles By similarity.By similarity

    GO - Cellular componenti

    1. contractile fiber Source: MGI
    2. cytoplasm Source: MGI
    3. intracellular Source: MGI
    4. nucleus Source: MGI
    5. plasma membrane Source: MGI
    6. proteasome complex Source: BHF-UCL
    7. spindle Source: UniProtKB-SubCell
    8. Z disc Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    No visible phoenotype. Mice are viable and fertile with no apparent morphological abnormalities in tissues under physiological conditions.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 209209Heat shock protein beta-1PRO_0000125928Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei15 – 151Phosphoserine; by MAPKAPK2, MAPKAPK3, PKA and PKC2 Publications
    Modified residuei27 – 271PhosphoserineBy similarity
    Modified residuei86 – 861Phosphoserine; by MAPKAPK2, MAPKAPK3, MAPKAPK5, PKA and PKC2 Publications
    Modified residuei87 – 871PhosphoserineBy similarity
    Modified residuei90 – 901PhosphoserineBy similarity
    Modified residuei127 – 1271N6-acetyllysineBy similarity
    Modified residuei203 – 2031PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation by MAPKAPK2 and MAPKAPK3 in response to stress leads to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair its chaperone activity and ability to protect against oxidative stress effectively. Phosphorylation by MAPKAPK5 in response to PKA stimulation induces F-actin rearrangement.4 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP14602.
    PaxDbiP14602.
    PRIDEiP14602.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00128522.
    IPI00468068.
    P14602.
    SWISS-2DPAGEP14602.
    UCD-2DPAGEP14602.

    PTM databases

    PhosphoSiteiP14602.

    Expressioni

    Gene expression databases

    BgeeiP14602.
    CleanExiMM_HSPB1.
    GenevestigatoriP14602.

    Interactioni

    Subunit structurei

    Associates with alpha- and beta-tubulin, microtubules and CRYAB. Interacts with HSPB8 By similarity. Interacts with HSPBAP1 and TGFB1I1.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi200449. 13 interactions.
    IntActiP14602. 7 interactions.
    MINTiMINT-4098074.
    STRINGi10090.ENSMUSP00000005077.

    Structurei

    3D structure databases

    DisProtiDP00142.
    ProteinModelPortaliP14602.
    SMRiP14602. Positions 20-195.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni74 – 209136Interaction with TGFB1I1By similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the small heat shock protein (HSP20) family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG307785.
    HOGENOMiHOG000233955.
    HOVERGENiHBG054766.
    InParanoidiP14602.
    KOiK04455.
    OMAiDQSFGMP.
    OrthoDBiEOG7WHHBK.
    PhylomeDBiP14602.
    TreeFamiTF105049.

    Family and domain databases

    Gene3Di2.60.40.790. 2 hits.
    InterProiIPR002068. a-crystallin/Hsp20_dom.
    IPR001436. Alpha-crystallin/HSP.
    IPR008978. HSP20-like_chaperone.
    [Graphical view]
    PfamiPF00011. HSP20. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036514. Sm_HSP_B1. 1 hit.
    PRINTSiPR00299. ACRYSTALLIN.
    SUPFAMiSSF49764. SSF49764. 1 hit.
    PROSITEiPS01031. HSP20. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform A (identifier: P14602-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTERRVPFSL LRSPSWEPFR DWYPAHSRLF DQAFGVPRLP DEWSQWFSAA    50
    GWPGYVRPLP AATAEGPAAV TLAAPAFSRA LNRQLSSGVS EIRQTADRWR 100
    VSLDVNHFAP EELTVKTKEG VVEITGKHEE RQDEHGYISR CFTRKYTLPP 150
    GVDPTLVSSS LSPEGTLTVE APLPKAVTQS AEITIPVTFE ARAQIGGPEA 200
    GKSEQSGAK 209
    Length:209
    Mass (Da):23,014
    Last modified:June 1, 1994 - v3
    Checksum:i31BD9FAF4E107C50
    GO
    Isoform B (identifier: P14602-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         61-72: Missing.

    Show »
    Length:197
    Mass (Da):21,961
    Checksum:i071D3C112509C01A
    GO
    Isoform C (identifier: P14602-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         37-70: Missing.

    Show »
    Length:175
    Mass (Da):19,380
    Checksum:iAA7E60872B1C485B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131S → T in AAA18335. (PubMed:8132504)Curated
    Sequence conflicti13 – 131S → T in AAA18336. (PubMed:8132504)Curated
    Sequence conflicti13 – 131S → T in AAA18337. (PubMed:8132504)Curated
    Sequence conflicti17 – 171E → G in AAA18335. (PubMed:8132504)Curated
    Sequence conflicti67 – 682PA → L in AAA18335. (PubMed:8132504)Curated
    Sequence conflicti99 – 991Missing in CAA32818. (PubMed:2645135)Curated
    Sequence conflicti99 – 991Missing in CAB37341. (PubMed:2645135)Curated
    Sequence conflicti108 – 1092FA → VI in CAA32818. (PubMed:2645135)Curated
    Sequence conflicti108 – 1092FA → VI in CAB37341. (PubMed:2645135)Curated
    Sequence conflicti141 – 1411C → Q AA sequence (PubMed:2645135)Curated
    Sequence conflicti181 – 1811A → T in CAA32818. (PubMed:2645135)Curated
    Sequence conflicti181 – 1811A → T in CAB37341. (PubMed:2645135)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei37 – 7034Missing in isoform C. 1 PublicationVSP_002423Add
    BLAST
    Alternative sequencei61 – 7212Missing in isoform B. 1 PublicationVSP_002422Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14687 mRNA. Translation: CAA32818.1.
    X14686 mRNA. Translation: CAB37341.1.
    L11609, L11608 Genomic DNA. Translation: AAA37862.1.
    L07577 Genomic DNA. Translation: AAA37861.1.
    U03560 mRNA. Translation: AAA18335.1.
    U03561 mRNA. Translation: AAA18336.1.
    U03562 mRNA. Translation: AAA18337.1.
    BC018257 mRNA. Translation: AAH18257.1.
    CCDSiCCDS39320.1. [P14602-1]
    PIRiA53423.
    I49763. JN0679.
    S02143.
    RefSeqiNP_038588.2. NM_013560.2. [P14602-1]
    UniGeneiMm.13849.

    Genome annotation databases

    EnsembliENSMUST00000005077; ENSMUSP00000005077; ENSMUSG00000004951. [P14602-1]
    GeneIDi15507.
    KEGGimmu:15507.
    UCSCiuc008zze.2. mouse. [P14602-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14687 mRNA. Translation: CAA32818.1 .
    X14686 mRNA. Translation: CAB37341.1 .
    L11609 , L11608 Genomic DNA. Translation: AAA37862.1 .
    L07577 Genomic DNA. Translation: AAA37861.1 .
    U03560 mRNA. Translation: AAA18335.1 .
    U03561 mRNA. Translation: AAA18336.1 .
    U03562 mRNA. Translation: AAA18337.1 .
    BC018257 mRNA. Translation: AAH18257.1 .
    CCDSi CCDS39320.1. [P14602-1 ]
    PIRi A53423.
    I49763. JN0679.
    S02143.
    RefSeqi NP_038588.2. NM_013560.2. [P14602-1 ]
    UniGenei Mm.13849.

    3D structure databases

    DisProti DP00142.
    ProteinModelPortali P14602.
    SMRi P14602. Positions 20-195.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200449. 13 interactions.
    IntActi P14602. 7 interactions.
    MINTi MINT-4098074.
    STRINGi 10090.ENSMUSP00000005077.

    PTM databases

    PhosphoSitei P14602.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00128522.
    IPI00468068.
    P14602.
    SWISS-2DPAGE P14602.
    UCD-2DPAGE P14602.

    Proteomic databases

    MaxQBi P14602.
    PaxDbi P14602.
    PRIDEi P14602.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005077 ; ENSMUSP00000005077 ; ENSMUSG00000004951 . [P14602-1 ]
    GeneIDi 15507.
    KEGGi mmu:15507.
    UCSCi uc008zze.2. mouse. [P14602-1 ]

    Organism-specific databases

    CTDi 3315.
    MGIi MGI:96240. Hspb1.

    Phylogenomic databases

    eggNOGi NOG307785.
    HOGENOMi HOG000233955.
    HOVERGENi HBG054766.
    InParanoidi P14602.
    KOi K04455.
    OMAi DQSFGMP.
    OrthoDBi EOG7WHHBK.
    PhylomeDBi P14602.
    TreeFami TF105049.

    Enzyme and pathway databases

    Reactomei REACT_198693. AUF1 (hnRNP D0) destabilizes mRNA.

    Miscellaneous databases

    NextBioi 288412.
    PROi P14602.
    SOURCEi Search...

    Gene expression databases

    Bgeei P14602.
    CleanExi MM_HSPB1.
    Genevestigatori P14602.

    Family and domain databases

    Gene3Di 2.60.40.790. 2 hits.
    InterProi IPR002068. a-crystallin/Hsp20_dom.
    IPR001436. Alpha-crystallin/HSP.
    IPR008978. HSP20-like_chaperone.
    [Graphical view ]
    Pfami PF00011. HSP20. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036514. Sm_HSP_B1. 1 hit.
    PRINTSi PR00299. ACRYSTALLIN.
    SUPFAMi SSF49764. SSF49764. 1 hit.
    PROSITEi PS01031. HSP20. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and organisation of a murine gene encoding small heat-shock protein Hsp25."
      Gaestel M., Gotthardt R., Mueller T.
      Gene 128:279-283(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
    2. "Cloning of the mouse hsp25 gene and an extremely conserved hsp25 pseudogene."
      Froehli E., Aoyama X., Klemenz R.
      Gene 128:273-277(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A).
    3. "Molecular cloning, sequencing and expression in Escherichia coli of the 25-kDa growth-related protein of Ehrlich ascites tumor and its homology to mammalian stress proteins."
      Gaestel M., Gross B., Benndorf R., Strauss M., Schunk W.-H., Kraft R., Otto A., Boehm H., Stahl J., Drabsch H., Bielka H.
      Eur. J. Biochem. 179:209-213(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM A), PARTIAL PROTEIN SEQUENCE.
    4. "Differential estrogenic regulation of small M(r) heat shock protein expression in osteoblasts."
      Cooper L.F., Uoshima K.
      J. Biol. Chem. 269:7869-7873(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B AND C).
      Tissue: Bone.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
      Strain: FVB/N.
      Tissue: Mammary gland.
    6. "Identification of the phosphorylation sites of the murine small heat shock protein hsp25."
      Gaestel M., Schroeder W., Benndorf R., Lippmann C., Buchner K., Hucho F., Erdmann V.A., Bielka H.
      J. Biol. Chem. 266:14721-14724(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-15 AND SER-86.
    7. "Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small mammalian heat shock proteins."
      Stokoe D., Engel K., Campbell D.G., Cohen P., Gaestel M.
      FEBS Lett. 313:307-313(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-15 AND SER-86 BY MAPKAPK2.
    8. "Small heat shock proteins are molecular chaperones."
      Jakob U., Gaestel M., Engel K., Buchner J.
      J. Biol. Chem. 268:1517-1520(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY MAPKAPK2.
    9. "Identification and characterization of a novel protein from Sertoli cells, PASS1, that associates with mammalian small stress protein hsp27."
      Liu C., Gilmont R.R., Benndorf R., Welsh M.J.
      J. Biol. Chem. 275:18724-18731(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HSPBAP1.
    10. "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein."
      Jia Y., Ransom R.F., Shibanuma M., Liu C., Welsh M.J., Smoyer W.E.
      J. Biol. Chem. 276:39911-39918(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TGFB1I1.
    11. "Insights into function and regulation of small heat shock protein 25 (HSPB1) in a mouse model with targeted gene disruption."
      Huang L., Min J.N., Masters S., Mivechi N.F., Moskophidis D.
      Genesis 45:487-501(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    13. "Distinct roles of MK2 and MK5 in cAMP/PKA- and stress/p38MAPK-induced heat shock protein 27 phosphorylation."
      Shiryaev A., Dumitriu G., Moens U.
      J. Mol. Signal. 6:4-4(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION BY MAPKAPK5.

    Entry informationi

    Entry nameiHSPB1_MOUSE
    AccessioniPrimary (citable) accession number: P14602
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1990
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 151 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3