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Protein

Beta-amyloid-like protein

Gene

Appl

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

During development, plays a role in the regulation of the neddylation pathway. Appl and APP-BP1 interact antagonistically during development.1 Publication

GO - Molecular functioni

GO - Biological processi

  • learning or memory Source: FlyBase
  • long-term memory Source: FlyBase
  • mushroom body development Source: FlyBase
  • negative regulation of neuron death Source: FlyBase
  • nervous system development Source: FlyBase
  • neuron projection morphogenesis Source: FlyBase
  • peripheral nervous system development Source: FlyBase
  • response to axon injury Source: FlyBase
  • response to electrical stimulus Source: FlyBase
  • short-term memory Source: FlyBase
  • synapse organization Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Enzyme and pathway databases

ReactomeiR-DME-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-amyloid-like protein
Gene namesi
Name:Appl
ORF Names:CG7727
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0000108. Appl.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 813786ExtracellularSequence analysisAdd
BLAST
Transmembranei814 – 83421HelicalSequence analysisAdd
BLAST
Topological domaini835 – 88753CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • extracellular region Source: FlyBase
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Membrane

Pathology & Biotechi

Disruption phenotypei

Overexpression inhibits the Nedd8 conjugation pathway, disrupts the normal bristle pattern in the fly thorax, and induces apoptosis in wing imaginal disks.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Chaini28 – 887860Beta-amyloid-like proteinPRO_0000000202Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi40 ↔ 70By similarity
Disulfide bondi81 ↔ 128By similarity
Disulfide bondi106 ↔ 116By similarity
Disulfide bondi143 ↔ 197By similarity
Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence analysis
Disulfide bondi154 ↔ 184By similarity
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence analysis
Disulfide bondi168 ↔ 196By similarity
Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence analysis
Glycosylationi240 – 2401N-linked (GlcNAc...)Sequence analysis
Glycosylationi574 – 5741N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP14599.

Expressioni

Tissue specificityi

Expressed in postmitotic neurons in the central and peripheral nervous systems. Within the nervous system, transcripts are not observed in neuroblasts, newly generated neurons and at least one class of presumed glial cells.2 Publications

Developmental stagei

Expressed in all developmental stages.2 Publications

Gene expression databases

BgeeiP14599.
ExpressionAtlasiP14599. differential.
GenevisibleiP14599. DM.

Interactioni

Subunit structurei

Interacts (via the intracellular domain, ICD) with APP-BP1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Aplip1Q9W0K06EBI-74135,EBI-74120
Fas2P340823EBI-74135,EBI-868243
Mapk8ip2Q9ERE92EBI-74135,EBI-74576From a different organism.
X11LQ9GQQ63EBI-74135,EBI-2027617
X11LQ9VX413EBI-74135,EBI-74153

Protein-protein interaction databases

BioGridi57571. 8 interactions.
IntActiP14599. 8 interactions.
STRINGi7227.FBpp0298292.

Structurei

3D structure databases

ProteinModelPortaliP14599.
SMRiP14599. Positions 33-198, 395-597, 851-886.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi877 – 8804Clathrin-bindingSequence analysis

Domaini

The clathrin-binding site is essential for its association with X11-alpha, -beta, and -gamma. The sequence specific recognition extends to peptide residues that are C-terminal to the NPXY motif. This interaction appears to be independent of phosphorylation (By similarity).By similarity

Sequence similaritiesi

Belongs to the APP family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3540. Eukaryota.
ENOG410ZW2A. LUCA.
GeneTreeiENSGT00530000063252.
InParanoidiP14599.
OrthoDBiEOG7T7GSF.
PhylomeDBiP14599.

Family and domain databases

Gene3Di3.30.1490.140. 1 hit.
3.90.570.10. 1 hit.
InterProiIPR011178. Amyloid_glyco_Cu-bd.
IPR024329. Amyloid_glyco_E2_domain.
IPR008154. Amyloid_glyco_extra.
IPR019744. Amyloid_glyco_extracell_CS.
IPR015849. Amyloid_glyco_heparin-bd.
IPR019745. Amyloid_glyco_intracell_CS.
IPR019543. APP_amyloid_C.
[Graphical view]
PfamiPF10515. APP_amyloid. 1 hit.
PF12924. APP_Cu_bd. 1 hit.
PF12925. APP_E2. 1 hit.
PF02177. APP_N. 1 hit.
[Graphical view]
SMARTiSM00006. A4_EXTRA. 1 hit.
[Graphical view]
SUPFAMiSSF109843. SSF109843. 1 hit.
SSF56491. SSF56491. 1 hit.
SSF89811. SSF89811. 1 hit.
PROSITEiPS00319. A4_EXTRA. 1 hit.
PS00320. A4_INTRA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCAALRRNLL LRSLWVVLAI GTAQVQAASP RWEPQIAVLC EAGQIYQPQY
60 70 80 90 100
LSEEGRWVTD LSKKTTGPTC LRDKMDLLDY CKKAYPNRDI TNIVESSHYQ
110 120 130 140 150
KIGGWCRQGA LNAAKCKGSH RWIKPFRCLG PFQSDALLVP EGCLFDHIHN
160 170 180 190 200
ASRCWPFVRW NQTGAAACQE RGMQMRSFAM LLPCGISVFS GVEFVCCPKH
210 220 230 240 250
FKTDEIHVKK TDLPVMPAAQ INSANDELVM NDEDDSNDSN YSKDANEDDL
260 270 280 290 300
DDEDDLMGDD EEDDMVADEA ATAGGSPNTG SSGDSNSGSL DDINAEYDSG
310 320 330 340 350
EEGDNYEEDG AGSESEAEVE ASWDQSGGAK VVSLKSDSSS PSSAPVAPAP
360 370 380 390 400
EKAPVKSESV TSTPQLSASA AAFVAANSGN SGTGAGAPPS TAQPTSDPYF
410 420 430 440 450
THFDPHYEHQ SYKVSQKRLE ESHREKVTRV MKDWSDLEEK YQDMRLADPK
460 470 480 490 500
AAQSFKQRMT ARFQTSVQAL EEEGNAEKHQ LAAMHQQRVL AHINQRKREA
510 520 530 540 550
MTCYTQALTE QPPNAHHVEK CLQKLLRALH KDRAHALAHY RHLLNSGGPG
560 570 580 590 600
GLEAAASERP RTLERLIDID RAVNQSMTML KRYPELSAKI AQLMNDYILA
610 620 630 640 650
LRSKDDIPGS SLGMSEEAEA GILDKYRVEI ERKVAEKERL RLAEKQRKEQ
660 670 680 690 700
RAAEREKLRE EKLRLEAKKV DDMLKSQVAE QQSQPTQSST QSQAQQQQQE
710 720 730 740 750
KSLPGKELGP DAALVTAANP NLETTKSEKD LSDTEYGEAT VSSTKVQTVL
760 770 780 790 800
PTVDDDAVQR AVEDVAAAVA HQEAEPQVQH FMTHDLGHRE SSFSLRREFA
810 820 830 840 850
QHAHAAKEGR NVYFTLSFAG IALMAAVFVG VAVAKWRTSR SPHAQGFIEV
860 870 880
DQNVTTHHPI VREEKIVPNM QINGYENPTY KYFEVKE
Length:887
Mass (Da):98,333
Last modified:November 28, 2002 - v2
Checksum:iF0F0855AD65A5275
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771S → T in AAA28874 (PubMed:2494667).Curated
Sequence conflicti229 – 2291Missing in AAA28874 (PubMed:2494667).Curated
Sequence conflicti332 – 3321V → M in CAA21409 (PubMed:10731137).Curated
Sequence conflicti332 – 3321V → M in CAA18093 (PubMed:10731137).Curated
Sequence conflicti743 – 7431S → T in AAA28874 (PubMed:2494667).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04516 Genomic DNA. Translation: AAA28874.1.
AE014298 Genomic DNA. Translation: AAF45520.2.
AL031883, AL022139 Genomic DNA. Translation: CAA21409.1.
AL022139, AL031883 Genomic DNA. Translation: CAA18093.1.
AF181628 mRNA. Translation: AAD55414.1.
X55774, X55775 Genomic DNA. Translation: CAA39294.1.
PIRiA32758.
RefSeqiNP_476626.2. NM_057278.5.
UniGeneiDm.4862.

Genome annotation databases

EnsemblMetazoaiFBtr0070109; FBpp0070104; FBgn0000108.
GeneIDi31002.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04516 Genomic DNA. Translation: AAA28874.1.
AE014298 Genomic DNA. Translation: AAF45520.2.
AL031883, AL022139 Genomic DNA. Translation: CAA21409.1.
AL022139, AL031883 Genomic DNA. Translation: CAA18093.1.
AF181628 mRNA. Translation: AAD55414.1.
X55774, X55775 Genomic DNA. Translation: CAA39294.1.
PIRiA32758.
RefSeqiNP_476626.2. NM_057278.5.
UniGeneiDm.4862.

3D structure databases

ProteinModelPortaliP14599.
SMRiP14599. Positions 33-198, 395-597, 851-886.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi57571. 8 interactions.
IntActiP14599. 8 interactions.
STRINGi7227.FBpp0298292.

Proteomic databases

PaxDbiP14599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0070109; FBpp0070104; FBgn0000108.
GeneIDi31002.

Organism-specific databases

CTDi31002.
FlyBaseiFBgn0000108. Appl.

Phylogenomic databases

eggNOGiKOG3540. Eukaryota.
ENOG410ZW2A. LUCA.
GeneTreeiENSGT00530000063252.
InParanoidiP14599.
OrthoDBiEOG7T7GSF.
PhylomeDBiP14599.

Enzyme and pathway databases

ReactomeiR-DME-114608. Platelet degranulation.

Miscellaneous databases

ChiTaRSiAppl. fly.
GenomeRNAii31002.
NextBioi771415.
PROiP14599.

Gene expression databases

BgeeiP14599.
ExpressionAtlasiP14599. differential.
GenevisibleiP14599. DM.

Family and domain databases

Gene3Di3.30.1490.140. 1 hit.
3.90.570.10. 1 hit.
InterProiIPR011178. Amyloid_glyco_Cu-bd.
IPR024329. Amyloid_glyco_E2_domain.
IPR008154. Amyloid_glyco_extra.
IPR019744. Amyloid_glyco_extracell_CS.
IPR015849. Amyloid_glyco_heparin-bd.
IPR019745. Amyloid_glyco_intracell_CS.
IPR019543. APP_amyloid_C.
[Graphical view]
PfamiPF10515. APP_amyloid. 1 hit.
PF12924. APP_Cu_bd. 1 hit.
PF12925. APP_E2. 1 hit.
PF02177. APP_N. 1 hit.
[Graphical view]
SMARTiSM00006. A4_EXTRA. 1 hit.
[Graphical view]
SUPFAMiSSF109843. SSF109843. 1 hit.
SSF56491. SSF56491. 1 hit.
SSF89811. SSF89811. 1 hit.
PROSITEiPS00319. A4_EXTRA. 1 hit.
PS00320. A4_INTRA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A Drosophila gene encoding a protein resembling the human beta-amyloid protein precursor."
    Rosen D.R., Martin-Morris L., Luo L., White K.
    Proc. Natl. Acad. Sci. U.S.A. 86:2478-2482(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Oregon-R.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Ovary.
  6. "The Drosophila transcript encoded by the beta-amyloid protein precursor-like gene is restricted to the nervous system."
    Martin-Morris L.E., White K.
    Development 110:185-195(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-83, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "Drosophila homolog of APP-BP1 (dAPP-BP1) interacts antagonistically with APPL during Drosophila development."
    Kim H.J., Kim S.H., Shim S.O., Park E., Kim C., Kim K., Tanouye M.A., Yim J.
    Cell Death Differ. 14:103-115(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH APP-BP1, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiA4_DROME
AccessioniPrimary (citable) accession number: P14599
Secondary accession number(s): Q9TVV0, Q9U4H3, Q9W5F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1990
Last sequence update: November 28, 2002
Last modified: May 11, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

Was originally (PubMed:2494667) thought to be vnd but further analysis (PubMed:2127912) has clearly shown that it corresponds to Appl.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.