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P14585

- LIN12_CAEEL

UniProt

P14585 - LIN12_CAEEL

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Protein

Protein lin-12

Gene

lin-12

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in several cell fate decisions that require cell-cell interactions. It is possible that lin-12 encodes a membrane-bound receptor for a signal that enables expression of the ventral uterine precursor cell fate. Activity in cell fate decisions and tumorigenesis is negatively regulated by sel-10.2 Publications

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. transmembrane signaling receptor activity Source: WormBase

GO - Biological processi

  1. cell-cell signaling involved in cell fate commitment Source: WormBase
  2. cell fate specification Source: WormBase
  3. dauer exit Source: WormBase
  4. nematode larval development Source: WormBase
  5. Notch signaling pathway Source: WormBase
  6. oviposition Source: WormBase
  7. vulval development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation

Enzyme and pathway databases

SignaLinkiP14585.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lin-12
Alternative name(s):
Abnormal cell lineage protein 12
Gene namesi
Name:lin-12
ORF Names:R107.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome III

Organism-specific databases

WormBaseiR107.8; CE00274; WBGene00003001; lin-12.

Subcellular locationi

GO - Cellular componenti

  1. apical plasma membrane Source: WormBase
  2. integral component of membrane Source: UniProtKB-KW
  3. nucleus Source: WormBase
  4. plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence AnalysisAdd
BLAST
Chaini16 – 14291414Protein lin-12PRO_0000007634Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ 35By similarity
Disulfide bondi29 ↔ 49By similarity
Glycosylationi41 – 411N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi51 ↔ 60By similarity
Disulfide bondi118 ↔ 129By similarity
Disulfide bondi123 ↔ 138By similarity
Disulfide bondi140 ↔ 149By similarity
Disulfide bondi156 ↔ 169By similarity
Disulfide bondi163 ↔ 178By similarity
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi180 ↔ 189By similarity
Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi205 ↔ 227By similarity
Disulfide bondi221 ↔ 234By similarity
Disulfide bondi236 ↔ 245By similarity
Disulfide bondi254 ↔ 264By similarity
Disulfide bondi259 ↔ 273By similarity
Disulfide bondi275 ↔ 284By similarity
Disulfide bondi291 ↔ 302By similarity
Disulfide bondi296 ↔ 311By similarity
Disulfide bondi313 ↔ 322By similarity
Disulfide bondi327 ↔ 339By similarity
Disulfide bondi334 ↔ 351By similarity
Disulfide bondi353 ↔ 362By similarity
Disulfide bondi369 ↔ 381By similarity
Disulfide bondi375 ↔ 390By similarity
Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi392 ↔ 401By similarity
Disulfide bondi408 ↔ 419By similarity
Disulfide bondi413 ↔ 429By similarity
Disulfide bondi431 ↔ 440By similarity
Disulfide bondi462 ↔ 475By similarity
Disulfide bondi469 ↔ 480By similarity
Disulfide bondi482 ↔ 491By similarity
Disulfide bondi507 ↔ 518By similarity
Disulfide bondi512 ↔ 529By similarity
Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi531 ↔ 540By similarity
Disulfide bondi547 ↔ 558By similarity
Disulfide bondi552 ↔ 567By similarity
Disulfide bondi569 ↔ 578By similarity
Disulfide bondi586 ↔ 597By similarity
Disulfide bondi591 ↔ 607By similarity
Disulfide bondi609 ↔ 618By similarity
Glycosylationi623 – 6231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi638 ↔ 661By similarity
Disulfide bondi643 ↔ 656By similarity
Disulfide bondi652 ↔ 668By similarity
Disulfide bondi678 ↔ 702By similarity
Disulfide bondi684 ↔ 697By similarity
Disulfide bondi693 ↔ 709By similarity
Disulfide bondi716 ↔ 742By similarity
Disulfide bondi724 ↔ 737By similarity
Disulfide bondi733 ↔ 749By similarity
Glycosylationi751 – 7511N-linked (GlcNAc...)Sequence Analysis
Glycosylationi754 – 7541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi900 – 9001N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP14585.

Interactioni

Subunit structurei

Interacts with sel-10.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
sel-10Q937943EBI-326049,EBI-323098

Protein-protein interaction databases

BioGridi41481. 19 interactions.
DIPiDIP-25208N.
IntActiP14585. 2 interactions.
MINTiMINT-117245.
STRINGi6239.R107.8.

Structurei

Secondary structure

1
1429
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi938 – 9414
Helixi1025 – 10317
Beta strandi1032 – 10343
Turni1042 – 10476
Helixi1056 – 10616
Helixi1072 – 108211
Helixi1097 – 11048
Helixi1107 – 11159
Helixi1130 – 11367
Helixi1140 – 11467
Helixi1150 – 11545
Helixi1166 – 11727
Helixi1178 – 118811
Helixi1197 – 11993
Beta strandi1201 – 12044
Helixi1211 – 12155
Helixi1220 – 122910
Helixi1244 – 12518
Helixi1254 – 12629
Beta strandi1272 – 12743
Helixi1277 – 12837
Helixi1287 – 12948

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FO1X-ray3.12E931-1301[»]
3BRDX-ray2.21D930-957[»]
3BRFX-ray2.47D938-950[»]
ProteinModelPortaliP14585.
SMRiP14585. Positions 20-807, 1015-1351.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14585.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini16 – 908893ExtracellularSequence AnalysisAdd
BLAST
Topological domaini932 – 1429498CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei909 – 93123HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 6142EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini114 – 15037EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini152 – 19039EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini201 – 24646EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini250 – 28536EGF-like 5PROSITE-ProRule annotationAdd
BLAST
Domaini287 – 32337EGF-like 6PROSITE-ProRule annotationAdd
BLAST
Domaini323 – 36341EGF-like 7PROSITE-ProRule annotationAdd
BLAST
Domaini365 – 40238EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini404 – 44138EGF-like 9PROSITE-ProRule annotationAdd
BLAST
Domaini449 – 49244EGF-like 10PROSITE-ProRule annotationAdd
BLAST
Domaini503 – 54139EGF-like 11PROSITE-ProRule annotationAdd
BLAST
Domaini543 – 57937EGF-like 12PROSITE-ProRule annotationAdd
BLAST
Domaini582 – 61938EGF-like 13PROSITE-ProRule annotationAdd
BLAST
Repeati638 – 67437LNR 1Add
BLAST
Repeati678 – 70932LNR 2Add
BLAST
Repeati716 – 75439LNR 3Add
BLAST
Repeati1093 – 112230ANK 1Add
BLAST
Repeati1126 – 115833ANK 2Add
BLAST
Repeati1162 – 119433ANK 3Add
BLAST
Repeati1206 – 123631ANK 4Add
BLAST
Repeati1240 – 126930ANK 5Add
BLAST

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation
Contains 13 EGF-like domains.PROSITE-ProRule annotation
Contains 3 LNR (Lin/Notch) repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00760000118786.
HOGENOMiHOG000112741.
InParanoidiP14585.
OMAiFNGGKCL.
OrthoDBiEOG7992RD.
PhylomeDBiP14585.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000800. Notch_dom.
IPR010660. Notch_NOD_dom.
IPR011656. Notch_NODP_dom.
[Graphical view]
PfamiPF00023. Ank. 3 hits.
PF00008. EGF. 5 hits.
PF07645. EGF_CA. 1 hit.
PF12661. hEGF. 2 hits.
PF06816. NOD. 1 hit.
PF07684. NODP. 1 hit.
PF00066. Notch. 3 hits.
[Graphical view]
PRINTSiPR01452. LNOTCHREPEAT.
SMARTiSM00248. ANK. 6 hits.
SM00181. EGF. 11 hits.
SM00179. EGF_CA. 2 hits.
SM00004. NL. 3 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF57184. SSF57184. 1 hit.
SSF90193. SSF90193. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 12 hits.
PS01186. EGF_2. 11 hits.
PS50026. EGF_3. 13 hits.
PS01187. EGF_CA. 2 hits.
PS50258. LNR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14585-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIPTICFLF LLISLSKSLH IGSCLGLICG RNGHCHAGPV NGTQTSYWCR
60 70 80 90 100
CDEGFGGEYC EQQCDVSKCG ADEKCVFDKD YRMETCVCKD CDINGNSLLK
110 120 130 140 150
PSCPSGYGGD DCKTQGWCYP SVCMNGGQCI GAGNRAKCAC PDGFKGERCE
160 170 180 190 200
LDVNECEENK NACGNRSTCM NTLGTYICVC PQGFLPPDCL KPGNTSTVEF
210 220 230 240 250
KQPVCFLEIS ADHPDGRSMY CQNGGFCDKA SSKCQCPPGY HGSTCELLEK
260 270 280 290 300
EDSCASNPCS HGVCISFSGG FQCICDDGYS GSYCQEGKDN CVNNKCEAGS
310 320 330 340 350
KCINGVNSYF CDCPPERTGP YCEKMDCSAI PDICNHGTCI DSPLSEKAFE
360 370 380 390 400
CQCEPGYEGI LCEQDKNECL SENMCLNNGT CVNLPGSFRC DCARGFGGKW
410 420 430 440 450
CDEPLNMCQD FHCENDGTCM HTSDHSPVCQ CKNGFIGKRC EKECPIGFGG
460 470 480 490 500
VRCDLRLEIG ICSRQGGKCF NGGKCLSGFC VCPPDFTGNQ CEVNRKNGKS
510 520 530 540 550
SLSENLCLSD PCMNNATCID VDAHIGYACI CKQGFEGDIC ERHKDLCLEN
560 570 580 590 600
PCSNGGVCHQ HRESFSCDCP PGFYGNGCEQ EKMFRCLKST CQNGGVCINE
610 620 630 640 650
EEKGRKCECS YGFSGARCEE KINLTGFTEK DSLLRSVCEK RKCSERANDG
660 670 680 690 700
NCDADCNYAA CKFDGGDCSG KREPFSKCRY GNMCADFFAN GVCNQACNNE
710 720 730 740 750
ECLYDGMDCL PAVVRCPVKI REHCASRFAN GICDPECNTN GCGFDGGDCD
760 770 780 790 800
NETNATIITN IRITVQMDPK EFQVTGGQSL MEISSALRVT VRIQRDEEGP
810 820 830 840 850
LVFQWNGESE MDRVKMNERQ LTEQHVLSTS ISRKIKRSAT NIGVVVYLEV
860 870 880 890 900
QENCDTGKCL YKDAQSVVDS ISARLAKKGI DSFGIPISEA LVAEPRKSGN
910 920 930 940 950
NTGFLSWNAL LLIGAGCLIV MVVLMLGALP GNRTRKRRMI NASVWMPPME
960 970 980 990 1000
NEEKNRKNHQ SITSSQHSLL EASYDGYIKR QRNELQHYSL YPNPQGYGNG
1010 1020 1030 1040 1050
NDFLGDFNHT NLQIPTEPEP ESPIKLHTEA AGSYAITEPI TRESVNIIDP
1060 1070 1080 1090 1100
RHNRTVLHWI ASNSSAEKSE DLIVHEAKEC IAAGADVNAM DCDENTPLML
1110 1120 1130 1140 1150
AVLARRRRLV AYLMKAGADP TIYNKSERSA LHQAAANRDF GMMVYMLNST
1160 1170 1180 1190 1200
KLKGDIEELD RNGMTALMIV AHNEGRDQVA SAKLLVEKGA KVDYDGAARK
1210 1220 1230 1240 1250
DSEKYKGRTA LHYAAQVSNM PIVKYLVGEK GSNKDKQDED GKTPIMLAAQ
1260 1270 1280 1290 1300
EGRIEVVMYL IQQGASVEAV DATDHTARQL AQANNHHNIV DIFDRCRPER
1310 1320 1330 1340 1350
EYSMDLHIQH THQPQPSRKV TRAPKKQTSR SKKESASNSR DSTHLTPPPS
1360 1370 1380 1390 1400
DGSTSTPSPQ HFMNTTHTTP TSLNYLSPEY QTEAGSSEAF QPQCGAFGNG
1410 1420
EMWYTRASTS YTQMQNEPMT RYSEPAHYF
Length:1,429
Mass (Da):157,116
Last modified:January 1, 1990 - v1
Checksum:i255EDD7A62C025DB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12069 Genomic DNA. Translation: AAA70191.1.
Z14092 Genomic DNA. Translation: CAA78474.1.
PIRiS06434.
RefSeqiNP_499007.1. NM_066606.3.
UniGeneiCel.10278.

Genome annotation databases

EnsemblMetazoaiR107.8; R107.8; WBGene00003001.
GeneIDi176282.
KEGGicel:CELE_R107.8.
UCSCiR107.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M12069 Genomic DNA. Translation: AAA70191.1 .
Z14092 Genomic DNA. Translation: CAA78474.1 .
PIRi S06434.
RefSeqi NP_499007.1. NM_066606.3.
UniGenei Cel.10278.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2FO1 X-ray 3.12 E 931-1301 [» ]
3BRD X-ray 2.21 D 930-957 [» ]
3BRF X-ray 2.47 D 938-950 [» ]
ProteinModelPortali P14585.
SMRi P14585. Positions 20-807, 1015-1351.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 41481. 19 interactions.
DIPi DIP-25208N.
IntActi P14585. 2 interactions.
MINTi MINT-117245.
STRINGi 6239.R107.8.

Proteomic databases

PaxDbi P14585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai R107.8 ; R107.8 ; WBGene00003001 .
GeneIDi 176282.
KEGGi cel:CELE_R107.8.
UCSCi R107.8. c. elegans.

Organism-specific databases

CTDi 176282.
WormBasei R107.8 ; CE00274 ; WBGene00003001 ; lin-12.

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00760000118786.
HOGENOMi HOG000112741.
InParanoidi P14585.
OMAi FNGGKCL.
OrthoDBi EOG7992RD.
PhylomeDBi P14585.

Enzyme and pathway databases

SignaLinki P14585.

Miscellaneous databases

EvolutionaryTracei P14585.
NextBioi 891914.
PROi P14585.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000800. Notch_dom.
IPR010660. Notch_NOD_dom.
IPR011656. Notch_NODP_dom.
[Graphical view ]
Pfami PF00023. Ank. 3 hits.
PF00008. EGF. 5 hits.
PF07645. EGF_CA. 1 hit.
PF12661. hEGF. 2 hits.
PF06816. NOD. 1 hit.
PF07684. NODP. 1 hit.
PF00066. Notch. 3 hits.
[Graphical view ]
PRINTSi PR01452. LNOTCHREPEAT.
SMARTi SM00248. ANK. 6 hits.
SM00181. EGF. 11 hits.
SM00179. EGF_CA. 2 hits.
SM00004. NL. 3 hits.
[Graphical view ]
SUPFAMi SSF48403. SSF48403. 1 hit.
SSF57184. SSF57184. 1 hit.
SSF90193. SSF90193. 3 hits.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 12 hits.
PS01186. EGF_2. 11 hits.
PS50026. EGF_3. 13 hits.
PS01187. EGF_CA. 2 hits.
PS50258. LNR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Caenorhabditis elegans lin-12 gene encodes a transmembrane protein with overall similarity to Drosophila Notch."
    Yochem J., Weston K., Greenwald I.
    Nature 335:547-550(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: Bristol N2.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  4. "lin-12, a nematode homeotic gene, is homologous to a set of mammalian proteins that includes epidermal growth factor."
    Greenwald I.
    Cell 43:583-590(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 173-712, FUNCTION.
  5. "sel-10, a negative regulator of lin-12 activity in Caenorhabditis elegans, encodes a member of the CDC4 family of proteins."
    Hubbard E.J.A., Wu G., Kitajewski J., Greenwald I.
    Genes Dev. 11:3182-3193(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SEL-10.

Entry informationi

Entry nameiLIN12_CAEEL
AccessioniPrimary (citable) accession number: P14585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3