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Protein

Protein lin-12

Gene

lin-12

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in several cell fate decisions that require cell-cell interactions. It is possible that lin-12 encodes a membrane-bound receptor for a signal that enables expression of the ventral uterine precursor cell fate. Activity in cell fate decisions and tumorigenesis is negatively regulated by sel-10.2 Publications

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • transmembrane signaling receptor activity Source: WormBase

GO - Biological processi

  • cell-cell signaling involved in cell fate commitment Source: WormBase
  • cell fate specification Source: WormBase
  • dauer exit Source: WormBase
  • nematode larval development Source: WormBase
  • Notch signaling pathway Source: WormBase
  • oviposition Source: WormBase
  • positive regulation of mesodermal cell fate specification Source: UniProtKB
  • vulval development Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation

Enzyme and pathway databases

SignaLinkiP14585.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein lin-12
Alternative name(s):
Abnormal cell lineage protein 12
Gene namesi
Name:lin-12
ORF Names:R107.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiR107.8; CE00274; WBGene00003001; lin-12.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini16 – 908ExtracellularSequence analysisAdd BLAST893
Transmembranei909 – 931HelicalSequence analysisAdd BLAST23
Topological domaini932 – 1429CytoplasmicSequence analysisAdd BLAST498

GO - Cellular componenti

  • apical plasma membrane Source: WormBase
  • integral component of membrane Source: UniProtKB-KW
  • nucleus Source: WormBase
  • plasma membrane Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
ChainiPRO_000000763416 – 1429Protein lin-12Add BLAST1414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24 ↔ 35By similarity
Disulfide bondi29 ↔ 49By similarity
Glycosylationi41N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi51 ↔ 60By similarity
Disulfide bondi118 ↔ 129By similarity
Disulfide bondi123 ↔ 138By similarity
Disulfide bondi140 ↔ 149By similarity
Disulfide bondi156 ↔ 169By similarity
Disulfide bondi163 ↔ 178By similarity
Glycosylationi165N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi180 ↔ 189By similarity
Glycosylationi194N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi205 ↔ 227By similarity
Disulfide bondi221 ↔ 234By similarity
Disulfide bondi236 ↔ 245By similarity
Disulfide bondi254 ↔ 264By similarity
Disulfide bondi259 ↔ 273By similarity
Disulfide bondi275 ↔ 284By similarity
Disulfide bondi291 ↔ 302By similarity
Disulfide bondi296 ↔ 311By similarity
Disulfide bondi313 ↔ 322By similarity
Disulfide bondi327 ↔ 339By similarity
Disulfide bondi334 ↔ 351By similarity
Disulfide bondi353 ↔ 362By similarity
Disulfide bondi369 ↔ 381By similarity
Disulfide bondi375 ↔ 390By similarity
Glycosylationi378N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi392 ↔ 401By similarity
Disulfide bondi408 ↔ 419By similarity
Disulfide bondi413 ↔ 429By similarity
Disulfide bondi431 ↔ 440By similarity
Disulfide bondi462 ↔ 475By similarity
Disulfide bondi469 ↔ 480By similarity
Disulfide bondi482 ↔ 491By similarity
Disulfide bondi507 ↔ 518By similarity
Disulfide bondi512 ↔ 529By similarity
Glycosylationi515N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi531 ↔ 540By similarity
Disulfide bondi547 ↔ 558By similarity
Disulfide bondi552 ↔ 567By similarity
Disulfide bondi569 ↔ 578By similarity
Disulfide bondi586 ↔ 597By similarity
Disulfide bondi591 ↔ 607By similarity
Disulfide bondi609 ↔ 618By similarity
Glycosylationi623N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi638 ↔ 661By similarity
Disulfide bondi643 ↔ 656By similarity
Disulfide bondi652 ↔ 668By similarity
Disulfide bondi678 ↔ 702By similarity
Disulfide bondi684 ↔ 697By similarity
Disulfide bondi693 ↔ 709By similarity
Disulfide bondi716 ↔ 742By similarity
Disulfide bondi724 ↔ 737By similarity
Disulfide bondi733 ↔ 749By similarity
Glycosylationi751N-linked (GlcNAc...)Sequence analysis1
Glycosylationi754N-linked (GlcNAc...)Sequence analysis1
Glycosylationi900N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP14585.

Expressioni

Gene expression databases

BgeeiWBGene00003001.

Interactioni

Subunit structurei

Interacts with sel-10.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
sel-10Q937943EBI-326049,EBI-323098

Protein-protein interaction databases

BioGridi41481. 19 interactors.
DIPiDIP-25208N.
IntActiP14585. 2 interactors.
MINTiMINT-117245.
STRINGi6239.R107.8.

Structurei

Secondary structure

11429
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi938 – 941Combined sources4
Helixi1025 – 1031Combined sources7
Beta strandi1032 – 1034Combined sources3
Turni1042 – 1047Combined sources6
Helixi1056 – 1061Combined sources6
Helixi1072 – 1082Combined sources11
Helixi1097 – 1104Combined sources8
Helixi1107 – 1115Combined sources9
Helixi1130 – 1136Combined sources7
Helixi1140 – 1146Combined sources7
Helixi1150 – 1154Combined sources5
Helixi1166 – 1172Combined sources7
Helixi1178 – 1188Combined sources11
Helixi1197 – 1199Combined sources3
Beta strandi1201 – 1204Combined sources4
Helixi1211 – 1215Combined sources5
Helixi1220 – 1229Combined sources10
Helixi1244 – 1251Combined sources8
Helixi1254 – 1262Combined sources9
Beta strandi1272 – 1274Combined sources3
Helixi1277 – 1283Combined sources7
Helixi1287 – 1294Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FO1X-ray3.12E931-1301[»]
3BRDX-ray2.21D930-957[»]
3BRFX-ray2.47D938-950[»]
ProteinModelPortaliP14585.
SMRiP14585.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14585.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 61EGF-like 1PROSITE-ProRule annotationAdd BLAST42
Domaini114 – 150EGF-like 2PROSITE-ProRule annotationAdd BLAST37
Domaini152 – 190EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini201 – 246EGF-like 4PROSITE-ProRule annotationAdd BLAST46
Domaini250 – 285EGF-like 5PROSITE-ProRule annotationAdd BLAST36
Domaini287 – 323EGF-like 6PROSITE-ProRule annotationAdd BLAST37
Domaini323 – 363EGF-like 7PROSITE-ProRule annotationAdd BLAST41
Domaini365 – 402EGF-like 8; calcium-bindingPROSITE-ProRule annotationAdd BLAST38
Domaini404 – 441EGF-like 9PROSITE-ProRule annotationAdd BLAST38
Domaini449 – 492EGF-like 10PROSITE-ProRule annotationAdd BLAST44
Domaini503 – 541EGF-like 11PROSITE-ProRule annotationAdd BLAST39
Domaini543 – 579EGF-like 12PROSITE-ProRule annotationAdd BLAST37
Domaini582 – 619EGF-like 13PROSITE-ProRule annotationAdd BLAST38
Repeati638 – 674LNR 1Add BLAST37
Repeati678 – 709LNR 2Add BLAST32
Repeati716 – 754LNR 3Add BLAST39
Repeati1093 – 1122ANK 1Add BLAST30
Repeati1126 – 1158ANK 2Add BLAST33
Repeati1162 – 1194ANK 3Add BLAST33
Repeati1206 – 1236ANK 4Add BLAST31
Repeati1240 – 1269ANK 5Add BLAST30

Sequence similaritiesi

Contains 5 ANK repeats.PROSITE-ProRule annotation
Contains 13 EGF-like domains.PROSITE-ProRule annotation
Contains 3 LNR (Lin/Notch) repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IR7G. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00810000125346.
HOGENOMiHOG000112741.
InParanoidiP14585.
OMAiRFANGIC.
OrthoDBiEOG091G01NU.
PhylomeDBiP14585.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
IPR000800. Notch_dom.
IPR010660. Notch_NOD_dom.
IPR011656. Notch_NODP_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
PF00008. EGF. 6 hits.
PF07645. EGF_CA. 1 hit.
PF12661. hEGF. 3 hits.
PF06816. NOD. 1 hit.
PF07684. NODP. 1 hit.
PF00066. Notch. 3 hits.
[Graphical view]
PRINTSiPR01452. LNOTCHREPEAT.
SMARTiSM00248. ANK. 6 hits.
SM00181. EGF. 13 hits.
SM00179. EGF_CA. 9 hits.
SM00004. NL. 3 hits.
SM01338. NOD. 1 hit.
SM01339. NODP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF57184. SSF57184. 1 hit.
SSF90193. SSF90193. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 12 hits.
PS01186. EGF_2. 11 hits.
PS50026. EGF_3. 13 hits.
PS01187. EGF_CA. 2 hits.
PS50258. LNR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14585-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIPTICFLF LLISLSKSLH IGSCLGLICG RNGHCHAGPV NGTQTSYWCR
60 70 80 90 100
CDEGFGGEYC EQQCDVSKCG ADEKCVFDKD YRMETCVCKD CDINGNSLLK
110 120 130 140 150
PSCPSGYGGD DCKTQGWCYP SVCMNGGQCI GAGNRAKCAC PDGFKGERCE
160 170 180 190 200
LDVNECEENK NACGNRSTCM NTLGTYICVC PQGFLPPDCL KPGNTSTVEF
210 220 230 240 250
KQPVCFLEIS ADHPDGRSMY CQNGGFCDKA SSKCQCPPGY HGSTCELLEK
260 270 280 290 300
EDSCASNPCS HGVCISFSGG FQCICDDGYS GSYCQEGKDN CVNNKCEAGS
310 320 330 340 350
KCINGVNSYF CDCPPERTGP YCEKMDCSAI PDICNHGTCI DSPLSEKAFE
360 370 380 390 400
CQCEPGYEGI LCEQDKNECL SENMCLNNGT CVNLPGSFRC DCARGFGGKW
410 420 430 440 450
CDEPLNMCQD FHCENDGTCM HTSDHSPVCQ CKNGFIGKRC EKECPIGFGG
460 470 480 490 500
VRCDLRLEIG ICSRQGGKCF NGGKCLSGFC VCPPDFTGNQ CEVNRKNGKS
510 520 530 540 550
SLSENLCLSD PCMNNATCID VDAHIGYACI CKQGFEGDIC ERHKDLCLEN
560 570 580 590 600
PCSNGGVCHQ HRESFSCDCP PGFYGNGCEQ EKMFRCLKST CQNGGVCINE
610 620 630 640 650
EEKGRKCECS YGFSGARCEE KINLTGFTEK DSLLRSVCEK RKCSERANDG
660 670 680 690 700
NCDADCNYAA CKFDGGDCSG KREPFSKCRY GNMCADFFAN GVCNQACNNE
710 720 730 740 750
ECLYDGMDCL PAVVRCPVKI REHCASRFAN GICDPECNTN GCGFDGGDCD
760 770 780 790 800
NETNATIITN IRITVQMDPK EFQVTGGQSL MEISSALRVT VRIQRDEEGP
810 820 830 840 850
LVFQWNGESE MDRVKMNERQ LTEQHVLSTS ISRKIKRSAT NIGVVVYLEV
860 870 880 890 900
QENCDTGKCL YKDAQSVVDS ISARLAKKGI DSFGIPISEA LVAEPRKSGN
910 920 930 940 950
NTGFLSWNAL LLIGAGCLIV MVVLMLGALP GNRTRKRRMI NASVWMPPME
960 970 980 990 1000
NEEKNRKNHQ SITSSQHSLL EASYDGYIKR QRNELQHYSL YPNPQGYGNG
1010 1020 1030 1040 1050
NDFLGDFNHT NLQIPTEPEP ESPIKLHTEA AGSYAITEPI TRESVNIIDP
1060 1070 1080 1090 1100
RHNRTVLHWI ASNSSAEKSE DLIVHEAKEC IAAGADVNAM DCDENTPLML
1110 1120 1130 1140 1150
AVLARRRRLV AYLMKAGADP TIYNKSERSA LHQAAANRDF GMMVYMLNST
1160 1170 1180 1190 1200
KLKGDIEELD RNGMTALMIV AHNEGRDQVA SAKLLVEKGA KVDYDGAARK
1210 1220 1230 1240 1250
DSEKYKGRTA LHYAAQVSNM PIVKYLVGEK GSNKDKQDED GKTPIMLAAQ
1260 1270 1280 1290 1300
EGRIEVVMYL IQQGASVEAV DATDHTARQL AQANNHHNIV DIFDRCRPER
1310 1320 1330 1340 1350
EYSMDLHIQH THQPQPSRKV TRAPKKQTSR SKKESASNSR DSTHLTPPPS
1360 1370 1380 1390 1400
DGSTSTPSPQ HFMNTTHTTP TSLNYLSPEY QTEAGSSEAF QPQCGAFGNG
1410 1420
EMWYTRASTS YTQMQNEPMT RYSEPAHYF
Length:1,429
Mass (Da):157,116
Last modified:January 1, 1990 - v1
Checksum:i255EDD7A62C025DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12069 Genomic DNA. Translation: AAA70191.1.
Z14092 Genomic DNA. Translation: CAA78474.1.
PIRiS06434.
RefSeqiNP_499007.1. NM_066606.3.
UniGeneiCel.10278.

Genome annotation databases

EnsemblMetazoaiR107.8; R107.8; WBGene00003001.
GeneIDi176282.
KEGGicel:CELE_R107.8.
UCSCiR107.8. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M12069 Genomic DNA. Translation: AAA70191.1.
Z14092 Genomic DNA. Translation: CAA78474.1.
PIRiS06434.
RefSeqiNP_499007.1. NM_066606.3.
UniGeneiCel.10278.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FO1X-ray3.12E931-1301[»]
3BRDX-ray2.21D930-957[»]
3BRFX-ray2.47D938-950[»]
ProteinModelPortaliP14585.
SMRiP14585.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi41481. 19 interactors.
DIPiDIP-25208N.
IntActiP14585. 2 interactors.
MINTiMINT-117245.
STRINGi6239.R107.8.

Proteomic databases

PaxDbiP14585.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiR107.8; R107.8; WBGene00003001.
GeneIDi176282.
KEGGicel:CELE_R107.8.
UCSCiR107.8. c. elegans.

Organism-specific databases

CTDi176282.
WormBaseiR107.8; CE00274; WBGene00003001; lin-12.

Phylogenomic databases

eggNOGiENOG410IR7G. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00810000125346.
HOGENOMiHOG000112741.
InParanoidiP14585.
OMAiRFANGIC.
OrthoDBiEOG091G01NU.
PhylomeDBiP14585.

Enzyme and pathway databases

SignaLinkiP14585.

Miscellaneous databases

EvolutionaryTraceiP14585.
PROiP14585.

Gene expression databases

BgeeiWBGene00003001.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
IPR000800. Notch_dom.
IPR010660. Notch_NOD_dom.
IPR011656. Notch_NODP_dom.
[Graphical view]
PfamiPF00023. Ank. 1 hit.
PF12796. Ank_2. 1 hit.
PF00008. EGF. 6 hits.
PF07645. EGF_CA. 1 hit.
PF12661. hEGF. 3 hits.
PF06816. NOD. 1 hit.
PF07684. NODP. 1 hit.
PF00066. Notch. 3 hits.
[Graphical view]
PRINTSiPR01452. LNOTCHREPEAT.
SMARTiSM00248. ANK. 6 hits.
SM00181. EGF. 13 hits.
SM00179. EGF_CA. 9 hits.
SM00004. NL. 3 hits.
SM01338. NOD. 1 hit.
SM01339. NODP. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF57184. SSF57184. 1 hit.
SSF90193. SSF90193. 3 hits.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
PS00010. ASX_HYDROXYL. 3 hits.
PS00022. EGF_1. 12 hits.
PS01186. EGF_2. 11 hits.
PS50026. EGF_3. 13 hits.
PS01187. EGF_CA. 2 hits.
PS50258. LNR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLIN12_CAEEL
AccessioniPrimary (citable) accession number: P14585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 166 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.