Reviewed, UniProtKB/Swiss-Prot P14579 (CP4A5_RABIT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 4A5
Alternative name(s):
    CYPIVA5
    Lauric acid omega-hydroxylase
    EC=1.14.15.3

Gene names

Name:

CYP4A5

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	Octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alkane 1-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 4	4	Probable		PRO_0000003573
	Chain	5 – 511	507	Cytochrome P450 4A5		PRO_0000003574
Regions
	Transmembrane	11 – 31	21	Potential
Sites
	Metal binding	458	1	Iron (heme axial ligand) By similarity
	Binding site	322	1	Heme (covalent; via 1 link) By similarity
Experimental info
	Sequence conflict	435	1	G → S in CAA40493. Ref.2
	Sequence conflict	477	1	V → L in CAA40493. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P14579-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 11D174BFC8BFA268	FASTA	511	58,357
	10 20 30 40 50 60 MSVSALSPTR LPGSLSGLLQ VAALLGLLLL LLKAAQLYLR RQWLLRALQQ FPCPPFHWLL 70 80 90 100 110 120 GHSREFQMNQ ELQQILKWVE KFPRACPHWI GGNKVRVQLY DPDYMKVILG RSDPKSRGSY 130 140 150 160 170 180 TFVAPWIGYG LLLLNGQPWF QHRRMLTPAF HYDILKPYVG LMVDSVQIML DKWEQLVSQD 190 200 210 220 230 240 SSLEVFQDIS LMTLDTIMKC AFSYQGSVQL DSRNSQSYIQ AVGDLNNLVF ARVRNIFHQS 250 260 270 280 290 300 DTIYRLSPEG RLSHRACQLA HEHTDRVIQQ RKAQLQQEGE LEKVRRKRRL DFLDVLLFAK 310 320 330 340 350 360 MENGSSLSDQ DLRAEVDTFM FEGHDTTASG VSWIFYALAT HPEHQHRCRE EIQGLLGDGA 370 380 390 400 410 420 SITWEHLDQM PYTTMCIKEA MRLYPPVPAI SRDLSSPVTF PDGRSLPKGF TVTLSIYGLH 430 440 450 460 470 480 HNPNVWPNPE VFDPGRFTPG SARHSHAFLP FSGGARNCIG KQFAMNELKV AVALTLVRFE 490 500 510 LLPDPTRIPK PTARLVLKSN NGIHLRLRKL Q

References

[1]	"Cloning and expression of three rabbit kidney cDNAs encoding lauric acid omega-hydroxylases." Johnson E.F., Walker D.L., Griffin K.J., Clark J.E., Okita R.T., Meurhoff A.S., Masters B.S.S. Biochemistry 29:873-879(1990) [PubMed: 2340280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"cDNA cloning and expression of the mRNA for cytochrome P-450kd which shows a fatty acid omega-hydroxylating activity." Yokotani N., Kusunose E., Sogawa K., Kawashima H., Kinosaki M., Kusunose M., Fujii-Kuriyama Y. Eur. J. Biochem. 196:531-536(1991) [PubMed: 2013275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M28655 mRNA. Translation: AAA31229.1. X57209 mRNA. Translation: CAA40493.1.
PIR	A34260.
3D structure databases
HSSP	HSSP built from PDB template 1JPZ based on UniProtKB P14779.
ModBase	Search...
Genome annotation databases
Ensembl	ENSOCUG00000015041. Oryctolagus cuniculus. [Contig view]
Phylogenomic databases
HOVERGEN	P14579.
Enzyme and pathway databases
BRENDA	1.14.15.3. 255.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP4A5_RABIT

Accession

Primary (citable) accession number: P14579

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)