P14576 (SRP54_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal recognition particle 54 kDa protein
Short name=SRP54

Gene names

Name:

Srp54

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).
Subunit structure	Interacts with RNPS1 By similarity. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.
Subcellular location	Nucleus speckle By similarity. Cytoplasm.
Domain	Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence. Ref.4
Sequence similarities	Belongs to the GTP-binding SRP family. SRP54 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Signal recognition particle
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding RNA-binding
Molecular function	Ribonucleoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC SRP-dependent cotranslational protein targeting to membrane Inferred from electronic annotation. Source: InterPro
Cellular_component	nuclear speck Inferred from electronic annotation. Source: UniProtKB-SubCell signal recognition particle, endoplasmic reticulum targeting Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	7S RNA binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P14576-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P14576-2)

The sequence of this isoform differs from the canonical sequence as follows:
     86-103: LVDPGVKAWTPTKGKQNV → VKVYQHYESINSVLLSPF
     104-504: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 504

504

Signal recognition particle 54 kDa protein

PRO_0000101194

Regions

Nucleotide binding

108 – 115

8

GTP By similarity

Nucleotide binding

190 – 194

5

GTP By similarity

Nucleotide binding

248 – 251

4

GTP By similarity

Region

1 – 295

295

G-domain

Region

296 – 504

209

M-domain

Natural variations

Alternative sequence

86 – 103

18

LVDPG…GKQNV → VKVYQHYESINSVLLSPF in isoform 2.

VSP_014953

Alternative sequence

104 – 504

401

Missing in isoform 2.

VSP_014954

Experimental info

Sequence conflict

205

1

M → R in CAA34386. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

504

Helix Strand Turn

Details...

Helix

5 – 19

15

Helix

25 – 40

16

Helix

46 – 57

12

Helix

69 – 85

17

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 79AD58BE6D1E89CA	FASTA	504	55,721
	10 20 30 40 50 60 MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK QLRENVKSAI 70 80 90 100 110 120 DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK QNVIMFVGLQ GSGKTTTCSK 130 140 150 160 170 180 LAYYYQRKGW KTCLICADTF RAGAFDQLKQ NATKARIPFY GSYTEMDPVI IASEGVEKFK 190 200 210 220 230 240 NENFEIIIVD TSGRHKQEDS LFEEMLQVSN AIQPDNIVYV MDASIGQACE AQAKAFKDKV 250 260 270 280 290 300 DVASVIVTKL DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI 310 320 330 340 350 360 EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG MIPGFGTDFM 370 380 390 400 410 420 SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP GRIQRVARGS GVSTRDVQEL 430 440 450 460 470 480 LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL 490 500 QSMMRQFQQG AAGNMKGMMG FNNM
	Isoform 2 [UniParc]. Checksum: 5B84FCFFD2ABEE15	FASTA	103	11,569
	10 20 30 40 50 60 MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK QLRENVKSAI 70 80 90 100 DLEEMASGLN KRKMIQHAVF KELVKVKVYQ HYESINSVLL SPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle." Bernstein H.D., Poritz M.A., Strub K., Hoben P.J., Brenner S., Walter P. Nature 340:482-486(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Egg, Embryo and Embryonic stem cell.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: FVB/N. Tissue: Mammary tumor.
[4]	"The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence." Zopf D., Bernstein H.D., Johnson A.E., Walter P. EMBO J. 9:4511-4517(1990) [PubMed] [Europe PMC] [Abstract] Cited for: M-DOMAIN RNA-BINDING.
[5]	"Structure of the signal recognition particle interacting with the elongation-arrested ribosome." Halic M., Becker T., Pool M.R., Spahn C.M.T., Grassucci R.A., Frank J., Beckmann R. Nature 427:808-814(2004) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS) OF 326-434 IN COMPLEX WITH 80S RIBOSOME.
[6]	"Solution structure of the N-terminal domain of mouse putative signal recoginition particle 54 (SRP54)." RIKEN structural genomics initiative (RSGI) Submitted (NOV-2004) to the PDB data bank Cited for: STRUCTURE BY NMR OF 4-89.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X16319 mRNA. Translation: CAA34386.1.
AK010716 mRNA. Translation: BAB27138.1.
AK011928 mRNA. Translation: BAB27921.1.
AK136033 mRNA. Translation: BAE22786.1.
BC019683 mRNA. Translation: AAH19683.1.

IPI

IPI00387377.
IPI00461270.

PIR

S05198.

RefSeq

NP_001093579.1. NM_001100109.1.
NP_036029.2. NM_011899.4.

UniGene

Mm.12848.
Mm.471968.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RY1	electron microscopy	12.00	W	326-434	[»]
1WGW	NMR	-	A	4-85	[»]

ProteinModelPortal

P14576.

SMR

P14576. Positions 8-488.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-247596.

STRING

10090.ENSMUSP00000021407.

PTM databases

PhosphoSite

P14576.

Proteomic databases

PaxDb

P14576.

PRIDE

P14576.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000021407; ENSMUSP00000021407; ENSMUSG00000073079.
ENSMUST00000110708; ENSMUSP00000106336; ENSMUSG00000079108.

GeneID

24067.
665155.

KEGG

mmu:24067.
mmu:665155.

UCSC

uc007nof.1. mouse.

Organism-specific databases

CTD

24067.
665155.

MGI

MGI:1346087. Srp54.

Phylogenomic databases

eggNOG

COG0541.

GeneTree

ENSGT00550000074824.

HOGENOM

HOG000036165.

HOVERGEN

HBG061072.

InParanoid

P14576.

KO

K03106.

OMA

TAGRHKE.

OrthoDB

EOG45MN54.

Gene expression databases

Bgee

P14576.

Genevestigator

P14576.

GermOnline

ENSMUSG00000021020. Mus musculus.

Family and domain databases

Gene3D

1.10.260.30. 1 hit.

InterPro

IPR003593. AAA+_ATPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]

Pfam

PF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]

SMART

SM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]

SUPFAM

SSF47446. Signal_recog_particle_SRP54_M. 1 hit.
SSF47364. SRP54. 1 hit.

TIGRFAMs

TIGR01425. SRP54_euk. 1 hit.

PROSITE

PS00300. SRP54. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14576.

NextBio

304041.

SOURCE

Search...

Entry information

Entry name

SRP54_MOUSE

Accession

Primary (citable) accession number: P14576
Secondary accession number(s): Q3UWX8, Q9CWH7, Q9D008

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	August 16, 2005
Last modified:	April 3, 2013
This is version 134 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program