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P14576

- SRP54_MOUSE

UniProt

P14576 - SRP54_MOUSE

Protein

Signal recognition particle 54 kDa protein

Gene

Srp54

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 2 (16 Aug 2005)
      Previous versions | rss
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    Functioni

    Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi108 – 1158GTPBy similarity
    Nucleotide bindingi190 – 1945GTPBy similarity
    Nucleotide bindingi248 – 2514GTPBy similarity

    GO - Molecular functioni

    1. 7S RNA binding Source: InterPro
    2. GTPase activity Source: InterPro
    3. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. SRP-dependent cotranslational protein targeting to membrane Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding, RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_196445. SRP-dependent cotranslational protein targeting to membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal recognition particle 54 kDa protein
    Short name:
    SRP54
    Gene namesi
    Name:Srp54
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1346087. Srp54.

    Subcellular locationi

    Nucleus speckle By similarity. Cytoplasm

    GO - Cellular componenti

    1. nuclear speck Source: UniProtKB-SubCell
    2. signal recognition particle, endoplasmic reticulum targeting Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cytoplasm, Nucleus, Signal recognition particle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Signal recognition particle 54 kDa proteinPRO_0000101194Add
    BLAST

    Proteomic databases

    MaxQBiP14576.
    PaxDbiP14576.
    PRIDEiP14576.

    PTM databases

    PhosphoSiteiP14576.

    Expressioni

    Gene expression databases

    BgeeiP14576.
    GenevestigatoriP14576.

    Interactioni

    Subunit structurei

    Interacts with RNPS1 By similarity. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.By similarity

    Protein-protein interaction databases

    BioGridi204879. 1 interaction.
    IntActiP14576. 2 interactions.
    MINTiMINT-247596.
    STRINGi10090.ENSMUSP00000021407.

    Structurei

    Secondary structure

    1
    504
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 1915
    Helixi25 – 4016
    Helixi46 – 5712
    Helixi69 – 8517

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1RY1electron microscopy12.00W326-434[»]
    1WGWNMR-A4-92[»]
    ProteinModelPortaliP14576.
    SMRiP14576. Positions 8-488.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14576.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 295295G-domainAdd
    BLAST
    Regioni296 – 504209M-domainAdd
    BLAST

    Domaini

    Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.

    Sequence similaritiesi

    Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0541.
    GeneTreeiENSGT00550000074824.
    HOGENOMiHOG000036165.
    HOVERGENiHBG061072.
    InParanoidiP14576.
    KOiK03106.
    OMAiLKQHKQM.
    OrthoDBiEOG747PHN.
    PhylomeDBiP14576.
    TreeFamiTF106249.

    Family and domain databases

    Gene3Di1.10.260.30. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00306. SRP54.
    InterProiIPR003593. AAA+_ATPase.
    IPR027417. P-loop_NTPase.
    IPR013822. Signal_recog_particl_SRP54_hlx.
    IPR004125. Signal_recog_particle_SRP54_M.
    IPR022941. SRP54.
    IPR006325. SRP54_euk.
    IPR000897. SRP54_GTPase_dom.
    [Graphical view]
    PfamiPF00448. SRP54. 1 hit.
    PF02881. SRP54_N. 1 hit.
    PF02978. SRP_SPB. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00962. SRP54. 1 hit.
    SM00963. SRP54_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF47446. SSF47446. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
    PROSITEiPS00300. SRP54. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P14576-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK    50
    QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK 100
    QNVIMFVGLQ GSGKTTTCSK LAYYYQRKGW KTCLICADTF RAGAFDQLKQ 150
    NATKARIPFY GSYTEMDPVI IASEGVEKFK NENFEIIIVD TSGRHKQEDS 200
    LFEEMLQVSN AIQPDNIVYV MDASIGQACE AQAKAFKDKV DVASVIVTKL 250
    DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI 300
    EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG 350
    MIPGFGTDFM SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP 400
    GRIQRVARGS GVSTRDVQEL LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV 450
    SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL QSMMRQFQQG AAGNMKGMMG 500
    FNNM 504
    Length:504
    Mass (Da):55,721
    Last modified:August 16, 2005 - v2
    Checksum:i79AD58BE6D1E89CA
    GO
    Isoform 2 (identifier: P14576-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         86-103: LVDPGVKAWTPTKGKQNV → VKVYQHYESINSVLLSPF
         104-504: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:103
    Mass (Da):11,569
    Checksum:i5B84FCFFD2ABEE15
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti205 – 2051M → R in CAA34386. (PubMed:2502718)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei86 – 10318LVDPG…GKQNV → VKVYQHYESINSVLLSPF in isoform 2. 1 PublicationVSP_014953Add
    BLAST
    Alternative sequencei104 – 504401Missing in isoform 2. 1 PublicationVSP_014954Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16319 mRNA. Translation: CAA34386.1.
    AK010716 mRNA. Translation: BAB27138.1.
    AK011928 mRNA. Translation: BAB27921.1.
    AK136033 mRNA. Translation: BAE22786.1.
    BC019683 mRNA. Translation: AAH19683.1.
    CCDSiCCDS36449.1. [P14576-1]
    CCDS49065.1. [P14576-1]
    PIRiS05198.
    RefSeqiNP_001093579.1. NM_001100109.1. [P14576-1]
    NP_036029.2. NM_011899.4. [P14576-1]
    UniGeneiMm.12848.
    Mm.471968.

    Genome annotation databases

    EnsembliENSMUST00000021407; ENSMUSP00000021407; ENSMUSG00000073079. [P14576-1]
    ENSMUST00000110708; ENSMUSP00000106336; ENSMUSG00000079108. [P14576-1]
    GeneIDi24067.
    665155.
    KEGGimmu:24067.
    mmu:665155.
    UCSCiuc007nof.1. mouse. [P14576-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16319 mRNA. Translation: CAA34386.1 .
    AK010716 mRNA. Translation: BAB27138.1 .
    AK011928 mRNA. Translation: BAB27921.1 .
    AK136033 mRNA. Translation: BAE22786.1 .
    BC019683 mRNA. Translation: AAH19683.1 .
    CCDSi CCDS36449.1. [P14576-1 ]
    CCDS49065.1. [P14576-1 ]
    PIRi S05198.
    RefSeqi NP_001093579.1. NM_001100109.1. [P14576-1 ]
    NP_036029.2. NM_011899.4. [P14576-1 ]
    UniGenei Mm.12848.
    Mm.471968.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1RY1 electron microscopy 12.00 W 326-434 [» ]
    1WGW NMR - A 4-92 [» ]
    ProteinModelPortali P14576.
    SMRi P14576. Positions 8-488.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204879. 1 interaction.
    IntActi P14576. 2 interactions.
    MINTi MINT-247596.
    STRINGi 10090.ENSMUSP00000021407.

    PTM databases

    PhosphoSitei P14576.

    Proteomic databases

    MaxQBi P14576.
    PaxDbi P14576.
    PRIDEi P14576.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021407 ; ENSMUSP00000021407 ; ENSMUSG00000073079 . [P14576-1 ]
    ENSMUST00000110708 ; ENSMUSP00000106336 ; ENSMUSG00000079108 . [P14576-1 ]
    GeneIDi 24067.
    665155.
    KEGGi mmu:24067.
    mmu:665155.
    UCSCi uc007nof.1. mouse. [P14576-1 ]

    Organism-specific databases

    CTDi 24067.
    665155.
    MGIi MGI:1346087. Srp54.

    Phylogenomic databases

    eggNOGi COG0541.
    GeneTreei ENSGT00550000074824.
    HOGENOMi HOG000036165.
    HOVERGENi HBG061072.
    InParanoidi P14576.
    KOi K03106.
    OMAi LKQHKQM.
    OrthoDBi EOG747PHN.
    PhylomeDBi P14576.
    TreeFami TF106249.

    Enzyme and pathway databases

    Reactomei REACT_196445. SRP-dependent cotranslational protein targeting to membrane.

    Miscellaneous databases

    EvolutionaryTracei P14576.
    NextBioi 304041.
    PROi P14576.
    SOURCEi Search...

    Gene expression databases

    Bgeei P14576.
    Genevestigatori P14576.

    Family and domain databases

    Gene3Di 1.10.260.30. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00306. SRP54.
    InterProi IPR003593. AAA+_ATPase.
    IPR027417. P-loop_NTPase.
    IPR013822. Signal_recog_particl_SRP54_hlx.
    IPR004125. Signal_recog_particle_SRP54_M.
    IPR022941. SRP54.
    IPR006325. SRP54_euk.
    IPR000897. SRP54_GTPase_dom.
    [Graphical view ]
    Pfami PF00448. SRP54. 1 hit.
    PF02881. SRP54_N. 1 hit.
    PF02978. SRP_SPB. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00962. SRP54. 1 hit.
    SM00963. SRP54_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47446. SSF47446. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR01425. SRP54_euk. 1 hit.
    PROSITEi PS00300. SRP54. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle."
      Bernstein H.D., Poritz M.A., Strub K., Hoben P.J., Brenner S., Walter P.
      Nature 340:482-486(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Egg, Embryo and Embryonic stem cell.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. "The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence."
      Zopf D., Bernstein H.D., Johnson A.E., Walter P.
      EMBO J. 9:4511-4517(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: M-DOMAIN RNA-BINDING.
    5. "Structure of the signal recognition particle interacting with the elongation-arrested ribosome."
      Halic M., Becker T., Pool M.R., Spahn C.M.T., Grassucci R.A., Frank J., Beckmann R.
      Nature 427:808-814(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS) OF 326-434 IN COMPLEX WITH 80S RIBOSOME.
    6. "Solution structure of the N-terminal domain of mouse putative signal recoginition particle 54 (SRP54)."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 4-89.

    Entry informationi

    Entry nameiSRP54_MOUSE
    AccessioniPrimary (citable) accession number: P14576
    Secondary accession number(s): Q3UWX8, Q9CWH7, Q9D008
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: August 16, 2005
    Last modified: October 1, 2014
    This is version 147 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3