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P14576 (SRP54_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal recognition particle 54 kDa protein

Short name=SRP54
Gene names
Name:Srp54
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). HAMAP-Rule MF_00306

Subunit structure

Interacts with RNPS1 By similarity. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9. HAMAP-Rule MF_00306

Subcellular location

Nucleus speckle By similarity. Cytoplasm HAMAP-Rule MF_00306.

Domain

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence. Ref.4

Sequence similarities

Belongs to the GTP-binding SRP family. SRP54 subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P14576-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P14576-2)

The sequence of this isoform differs from the canonical sequence as follows:
     86-103: LVDPGVKAWTPTKGKQNV → VKVYQHYESINSVLLSPF
     104-504: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Signal recognition particle 54 kDa protein HAMAP-Rule MF_00306
PRO_0000101194

Regions

Nucleotide binding108 – 1158GTP By similarity
Nucleotide binding190 – 1945GTP By similarity
Nucleotide binding248 – 2514GTP By similarity
Region1 – 295295G-domain HAMAP-Rule MF_00306
Region296 – 504209M-domain HAMAP-Rule MF_00306

Natural variations

Alternative sequence86 – 10318LVDPG…GKQNV → VKVYQHYESINSVLLSPF in isoform 2.
VSP_014953
Alternative sequence104 – 504401Missing in isoform 2.
VSP_014954

Experimental info

Sequence conflict2051M → R in CAA34386. Ref.1

Secondary structure

......... 504
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 16, 2005. Version 2.
Checksum: 79AD58BE6D1E89CA

FASTA50455,721
        10         20         30         40         50         60 
MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK QLRENVKSAI 

        70         80         90        100        110        120 
DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK QNVIMFVGLQ GSGKTTTCSK 

       130        140        150        160        170        180 
LAYYYQRKGW KTCLICADTF RAGAFDQLKQ NATKARIPFY GSYTEMDPVI IASEGVEKFK 

       190        200        210        220        230        240 
NENFEIIIVD TSGRHKQEDS LFEEMLQVSN AIQPDNIVYV MDASIGQACE AQAKAFKDKV 

       250        260        270        280        290        300 
DVASVIVTKL DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI 

       310        320        330        340        350        360 
EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG MIPGFGTDFM 

       370        380        390        400        410        420 
SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP GRIQRVARGS GVSTRDVQEL 

       430        440        450        460        470        480 
LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL 

       490        500 
QSMMRQFQQG AAGNMKGMMG FNNM 

« Hide

Isoform 2 [UniParc].

Checksum: 5B84FCFFD2ABEE15
Show »

FASTA10311,569

References

« Hide 'large scale' references
[1]"Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle."
Bernstein H.D., Poritz M.A., Strub K., Hoben P.J., Brenner S., Walter P.
Nature 340:482-486(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Egg, Embryo and Embryonic stem cell.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
[4]"The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence."
Zopf D., Bernstein H.D., Johnson A.E., Walter P.
EMBO J. 9:4511-4517(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: M-DOMAIN RNA-BINDING.
[5]"Structure of the signal recognition particle interacting with the elongation-arrested ribosome."
Halic M., Becker T., Pool M.R., Spahn C.M.T., Grassucci R.A., Frank J., Beckmann R.
Nature 427:808-814(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS) OF 326-434 IN COMPLEX WITH 80S RIBOSOME.
[6]"Solution structure of the N-terminal domain of mouse putative signal recoginition particle 54 (SRP54)."
RIKEN structural genomics initiative (RSGI)
Submitted (NOV-2004) to the PDB data bank
Cited for: STRUCTURE BY NMR OF 4-89.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16319 mRNA. Translation: CAA34386.1.
AK010716 mRNA. Translation: BAB27138.1.
AK011928 mRNA. Translation: BAB27921.1.
AK136033 mRNA. Translation: BAE22786.1.
BC019683 mRNA. Translation: AAH19683.1.
CCDSCCDS36449.1. [P14576-1]
CCDS49065.1. [P14576-1]
PIRS05198.
RefSeqNP_001093579.1. NM_001100109.1. [P14576-1]
NP_036029.2. NM_011899.4. [P14576-1]
UniGeneMm.12848.
Mm.471968.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1RY1electron microscopy12.00W326-434[»]
1WGWNMR-A4-92[»]
ProteinModelPortalP14576.
SMRP14576. Positions 8-488.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204879. 1 interaction.
IntActP14576. 2 interactions.
MINTMINT-247596.
STRING10090.ENSMUSP00000021407.

PTM databases

PhosphoSiteP14576.

Proteomic databases

MaxQBP14576.
PaxDbP14576.
PRIDEP14576.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021407; ENSMUSP00000021407; ENSMUSG00000073079. [P14576-1]
ENSMUST00000110708; ENSMUSP00000106336; ENSMUSG00000079108. [P14576-1]
GeneID24067.
665155.
KEGGmmu:24067.
mmu:665155.
UCSCuc007nof.1. mouse. [P14576-1]

Organism-specific databases

CTD24067.
665155.
MGIMGI:1346087. Srp54.

Phylogenomic databases

eggNOGCOG0541.
GeneTreeENSGT00550000074824.
HOGENOMHOG000036165.
HOVERGENHBG061072.
InParanoidP14576.
KOK03106.
OMALKQHKQM.
OrthoDBEOG747PHN.
PhylomeDBP14576.
TreeFamTF106249.

Gene expression databases

BgeeP14576.
GenevestigatorP14576.

Family and domain databases

Gene3D1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00306. SRP54.
InterProIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMSSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR01425. SRP54_euk. 1 hit.
PROSITEPS00300. SRP54. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP14576.
NextBio304041.
PROP14576.
SOURCESearch...

Entry information

Entry nameSRP54_MOUSE
AccessionPrimary (citable) accession number: P14576
Secondary accession number(s): Q3UWX8, Q9CWH7, Q9D008
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: August 16, 2005
Last modified: July 9, 2014
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot