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P14576

- SRP54_MOUSE

UniProt

P14576 - SRP54_MOUSE

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Protein

Signal recognition particle 54 kDa protein

Gene
Srp54
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi108 – 1158GTP By similarity
Nucleotide bindingi190 – 1945GTP By similarity
Nucleotide bindingi248 – 2514GTP By similarity

GO - Molecular functioni

  1. 7S RNA binding Source: InterPro
  2. GTPase activity Source: InterPro
  3. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. SRP-dependent cotranslational protein targeting to membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_196445. SRP-dependent cotranslational protein targeting to membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal recognition particle 54 kDa protein
Short name:
SRP54
Gene namesi
Name:Srp54
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1346087. Srp54.

Subcellular locationi

Nucleus speckle By similarity. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. nuclear speck Source: UniProtKB-SubCell
  2. signal recognition particle, endoplasmic reticulum targeting Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Signal recognition particle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Signal recognition particle 54 kDa proteinUniRule annotationPRO_0000101194Add
BLAST

Proteomic databases

MaxQBiP14576.
PaxDbiP14576.
PRIDEiP14576.

PTM databases

PhosphoSiteiP14576.

Expressioni

Gene expression databases

BgeeiP14576.
GenevestigatoriP14576.

Interactioni

Subunit structurei

Interacts with RNPS1 By similarity. Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.UniRule annotation

Protein-protein interaction databases

BioGridi204879. 1 interaction.
IntActiP14576. 2 interactions.
MINTiMINT-247596.
STRINGi10090.ENSMUSP00000021407.

Structurei

Secondary structure

1
504
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1915
Helixi25 – 4016
Helixi46 – 5712
Helixi69 – 8517

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RY1electron microscopy12.00W326-434[»]
1WGWNMR-A4-92[»]
ProteinModelPortaliP14576.
SMRiP14576. Positions 8-488.

Miscellaneous databases

EvolutionaryTraceiP14576.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 295295G-domainUniRule annotationAdd
BLAST
Regioni296 – 504209M-domainUniRule annotationAdd
BLAST

Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0541.
GeneTreeiENSGT00550000074824.
HOGENOMiHOG000036165.
HOVERGENiHBG061072.
InParanoidiP14576.
KOiK03106.
OMAiLKQHKQM.
OrthoDBiEOG747PHN.
PhylomeDBiP14576.
TreeFamiTF106249.

Family and domain databases

Gene3Di1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00306. SRP54.
InterProiIPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view]
PfamiPF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view]
SUPFAMiSSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01425. SRP54_euk. 1 hit.
PROSITEiPS00300. SRP54. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P14576-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVLADLGRKI TSALRSLSNA TIINEEVLNA MLKEVCTALL EADVNIKLVK    50
QLRENVKSAI DLEEMASGLN KRKMIQHAVF KELVKLVDPG VKAWTPTKGK 100
QNVIMFVGLQ GSGKTTTCSK LAYYYQRKGW KTCLICADTF RAGAFDQLKQ 150
NATKARIPFY GSYTEMDPVI IASEGVEKFK NENFEIIIVD TSGRHKQEDS 200
LFEEMLQVSN AIQPDNIVYV MDASIGQACE AQAKAFKDKV DVASVIVTKL 250
DGHAKGGGAL SAVAATKSPI IFIGTGEHID DFEPFKTQPF ISKLLGMGDI 300
EGLIDKVNEL KLDDNEALIE KLKHGQFTLR DMYEQFQNIM KMGPFSQILG 350
MIPGFGTDFM SKGNEQESMA RLKKLMTIMD SMNDQELDST DGAKVFSKQP 400
GRIQRVARGS GVSTRDVQEL LTQYTKFAQM VKKMGGIKGL FKGGDMSKNV 450
SQSQMAKLNQ QMAKMMDPRV LHHMGGMAGL QSMMRQFQQG AAGNMKGMMG 500
FNNM 504
Length:504
Mass (Da):55,721
Last modified:August 16, 2005 - v2
Checksum:i79AD58BE6D1E89CA
GO
Isoform 2 (identifier: P14576-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     86-103: LVDPGVKAWTPTKGKQNV → VKVYQHYESINSVLLSPF
     104-504: Missing.

Note: No experimental confirmation available.

Show »
Length:103
Mass (Da):11,569
Checksum:i5B84FCFFD2ABEE15
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei86 – 10318LVDPG…GKQNV → VKVYQHYESINSVLLSPF in isoform 2. VSP_014953Add
BLAST
Alternative sequencei104 – 504401Missing in isoform 2. VSP_014954Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti205 – 2051M → R in CAA34386. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16319 mRNA. Translation: CAA34386.1.
AK010716 mRNA. Translation: BAB27138.1.
AK011928 mRNA. Translation: BAB27921.1.
AK136033 mRNA. Translation: BAE22786.1.
BC019683 mRNA. Translation: AAH19683.1.
CCDSiCCDS36449.1. [P14576-1]
CCDS49065.1. [P14576-1]
PIRiS05198.
RefSeqiNP_001093579.1. NM_001100109.1. [P14576-1]
NP_036029.2. NM_011899.4. [P14576-1]
UniGeneiMm.12848.
Mm.471968.

Genome annotation databases

EnsembliENSMUST00000021407; ENSMUSP00000021407; ENSMUSG00000073079. [P14576-1]
ENSMUST00000110708; ENSMUSP00000106336; ENSMUSG00000079108. [P14576-1]
GeneIDi24067.
665155.
KEGGimmu:24067.
mmu:665155.
UCSCiuc007nof.1. mouse. [P14576-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16319 mRNA. Translation: CAA34386.1 .
AK010716 mRNA. Translation: BAB27138.1 .
AK011928 mRNA. Translation: BAB27921.1 .
AK136033 mRNA. Translation: BAE22786.1 .
BC019683 mRNA. Translation: AAH19683.1 .
CCDSi CCDS36449.1. [P14576-1 ]
CCDS49065.1. [P14576-1 ]
PIRi S05198.
RefSeqi NP_001093579.1. NM_001100109.1. [P14576-1 ]
NP_036029.2. NM_011899.4. [P14576-1 ]
UniGenei Mm.12848.
Mm.471968.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RY1 electron microscopy 12.00 W 326-434 [» ]
1WGW NMR - A 4-92 [» ]
ProteinModelPortali P14576.
SMRi P14576. Positions 8-488.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204879. 1 interaction.
IntActi P14576. 2 interactions.
MINTi MINT-247596.
STRINGi 10090.ENSMUSP00000021407.

PTM databases

PhosphoSitei P14576.

Proteomic databases

MaxQBi P14576.
PaxDbi P14576.
PRIDEi P14576.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021407 ; ENSMUSP00000021407 ; ENSMUSG00000073079 . [P14576-1 ]
ENSMUST00000110708 ; ENSMUSP00000106336 ; ENSMUSG00000079108 . [P14576-1 ]
GeneIDi 24067.
665155.
KEGGi mmu:24067.
mmu:665155.
UCSCi uc007nof.1. mouse. [P14576-1 ]

Organism-specific databases

CTDi 24067.
665155.
MGIi MGI:1346087. Srp54.

Phylogenomic databases

eggNOGi COG0541.
GeneTreei ENSGT00550000074824.
HOGENOMi HOG000036165.
HOVERGENi HBG061072.
InParanoidi P14576.
KOi K03106.
OMAi LKQHKQM.
OrthoDBi EOG747PHN.
PhylomeDBi P14576.
TreeFami TF106249.

Enzyme and pathway databases

Reactomei REACT_196445. SRP-dependent cotranslational protein targeting to membrane.

Miscellaneous databases

EvolutionaryTracei P14576.
NextBioi 304041.
PROi P14576.
SOURCEi Search...

Gene expression databases

Bgeei P14576.
Genevestigatori P14576.

Family and domain databases

Gene3Di 1.10.260.30. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00306. SRP54.
InterProi IPR003593. AAA+_ATPase.
IPR027417. P-loop_NTPase.
IPR013822. Signal_recog_particl_SRP54_hlx.
IPR004125. Signal_recog_particle_SRP54_M.
IPR022941. SRP54.
IPR006325. SRP54_euk.
IPR000897. SRP54_GTPase_dom.
[Graphical view ]
Pfami PF00448. SRP54. 1 hit.
PF02881. SRP54_N. 1 hit.
PF02978. SRP_SPB. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
SM00962. SRP54. 1 hit.
SM00963. SRP54_N. 1 hit.
[Graphical view ]
SUPFAMi SSF47446. SSF47446. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01425. SRP54_euk. 1 hit.
PROSITEi PS00300. SRP54. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle."
    Bernstein H.D., Poritz M.A., Strub K., Hoben P.J., Brenner S., Walter P.
    Nature 340:482-486(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PARTIAL PROTEIN SEQUENCE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Egg, Embryo and Embryonic stem cell.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "The methionine-rich domain of the 54 kd protein subunit of the signal recognition particle contains an RNA binding site and can be crosslinked to a signal sequence."
    Zopf D., Bernstein H.D., Johnson A.E., Walter P.
    EMBO J. 9:4511-4517(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: M-DOMAIN RNA-BINDING.
  5. "Structure of the signal recognition particle interacting with the elongation-arrested ribosome."
    Halic M., Becker T., Pool M.R., Spahn C.M.T., Grassucci R.A., Frank J., Beckmann R.
    Nature 427:808-814(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (12.0 ANGSTROMS) OF 326-434 IN COMPLEX WITH 80S RIBOSOME.
  6. "Solution structure of the N-terminal domain of mouse putative signal recoginition particle 54 (SRP54)."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2004) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 4-89.

Entry informationi

Entry nameiSRP54_MOUSE
AccessioniPrimary (citable) accession number: P14576
Secondary accession number(s): Q3UWX8, Q9CWH7, Q9D008
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: August 16, 2005
Last modified: September 3, 2014
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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