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P14568 (ASSY_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:ASS1
Synonyms:ASS
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Is indirectly involved in the control of blood pressure By similarity. HAMAP-Rule MF_00005

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1. HAMAP-Rule MF_00005

Subunit structure

Homotetramer. Interacts with NMRAL1 By similarity. HAMAP-Rule MF_00005

Involvement in disease

Defects in ASS1 are the cause of a bovine form of citrullinemia.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
Urea cycle
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

urea cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000148553

Regions

Nucleotide binding10 – 189ATP By similarity
Nucleotide binding115 – 1239ATP By similarity

Sites

Binding site361ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site871Citrulline By similarity
Binding site921Citrulline By similarity
Binding site1191Aspartate By similarity
Binding site1231Aspartate By similarity
Binding site1231Citrulline By similarity
Binding site1241Aspartate By similarity
Binding site1271Citrulline By similarity
Binding site1801Citrulline By similarity
Binding site1891Citrulline By similarity
Binding site2701Citrulline By similarity
Binding site2821Citrulline By similarity

Amino acid modifications

Modified residue1801Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P14568 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 6F74C7F445EE0D86

FASTA41246,417
        10         20         30         40         50         60 
MSGKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF 

        70         80         90        100        110        120 
IEDISKEFVE EFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG 

       130        140        150        160        170        180 
KGNDQIRFEL TCYSLAPQIK VIAPWRMPEF YNRFQGRNDL MEYAKQHGIP VPVTPKNPWS 

       190        200        210        220        230        240 
MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNSP DMLEIEFKKG VPVKVTNVGD 

       250        260        270        280        290        300 
GTTHSTALEL FLYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF 

       310        320        330        340        350        360 
TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVFKGQVYI 

       370        380        390        400        410 
LGRESPLSLY NEELVSMNVQ GDYEPVDATG FININSLRLK EYHRLQNKVT AK 

« Hide

References

« Hide 'large scale' references
[1]"Molecular definition of bovine argininosuccinate synthetase deficiency."
Dennis J.A., Healy P.J., Beaudet A.L., O'Brien W.E.
Proc. Natl. Acad. Sci. U.S.A. 86:7947-7951(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Ileum.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M26198 mRNA. Translation: AAA30388.1.
BC102474 mRNA. Translation: AAI02475.1.
PIRAJBORS. A33986.
RefSeqNP_776317.1. NM_173892.4.
UniGeneBt.1370.

3D structure databases

ProteinModelPortalP14568.
SMRP14568. Positions 4-407.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP14568.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000027649; ENSBTAP00000027649; ENSBTAG00000020747.
GeneID280726.
KEGGbta:280726.

Organism-specific databases

CTD445.

Phylogenomic databases

eggNOGCOG0137.
GeneTreeENSGT00390000004524.
HOGENOMHOG000230093.
HOVERGENHBG001717.
InParanoidP14568.
KOK01940.
OMAIYNGYWW.
OrthoDBEOG7PVWPB.
TreeFamTF300736.

Enzyme and pathway databases

SABIO-RKP14568.
UniPathwayUPA00068; UER00113.
UPA00158; UER00272.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20804902.

Entry information

Entry nameASSY_BOVIN
AccessionPrimary (citable) accession number: P14568
Secondary accession number(s): Q3T0A7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways