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Protein

Argininosuccinate synthase

Gene

ASS1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Is indirectly involved in the control of blood pressure.By similarity

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei87 – 871CitrullineBy similarity
Binding sitei92 – 921CitrullineBy similarity
Binding sitei119 – 1191AspartateBy similarity
Binding sitei123 – 1231AspartateBy similarity
Binding sitei123 – 1231CitrullineBy similarity
Binding sitei124 – 1241AspartateBy similarity
Binding sitei127 – 1271CitrullineBy similarity
Binding sitei180 – 1801CitrullineBy similarity
Binding sitei189 – 1891CitrullineBy similarity
Binding sitei270 – 2701CitrullineBy similarity
Binding sitei282 – 2821CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPBy similarity
Nucleotide bindingi115 – 1239ATPBy similarity

GO - Molecular functioni

  1. amino acid binding Source: Ensembl
  2. argininosuccinate synthase activity Source: GO_Central
  3. ATP binding Source: UniProtKB-KW
  4. poly(A) RNA binding Source: Ensembl

GO - Biological processi

  1. arginine biosynthetic process Source: GO_Central
  2. argininosuccinate metabolic process Source: GO_Central
  3. aspartate metabolic process Source: Ensembl
  4. cellular response to laminar fluid shear stress Source: Ensembl
  5. citrulline metabolic process Source: Ensembl
  6. negative regulation of leukocyte cell-cell adhesion Source: Ensembl
  7. positive regulation of nitric oxide biosynthetic process Source: Ensembl
  8. urea cycle Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_208559. Urea cycle.
SABIO-RKP14568.
UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:ASS1
Synonyms:ASS
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 11

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Pathology & Biotechi

Involvement in diseasei

Defects in ASS1 are the cause of a bovine form of citrullinemia.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 412412Argininosuccinate synthasePRO_0000148553Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei113 – 1131PhosphotyrosineBy similarity
Modified residuei180 – 1801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP14568.

Expressioni

Gene expression databases

ExpressionAtlasiP14568. baseline.

Interactioni

Subunit structurei

Homotetramer. Interacts with NMRAL1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP14568.
SMRiP14568. Positions 4-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
GeneTreeiENSGT00390000004524.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiP14568.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG7PVWPB.
TreeFamiTF300736.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14568-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK
60 70 80 90 100
ALKLGAKKVF IEDISKEFVE EFIWPAIQSS ALYEDRYLLG TSLARPCIAR
110 120 130 140 150
KQVEIAQREG AKYVSHGATG KGNDQIRFEL TCYSLAPQIK VIAPWRMPEF
160 170 180 190 200
YNRFQGRNDL MEYAKQHGIP VPVTPKNPWS MDENLMHISY EAGILENPKN
210 220 230 240 250
QAPPGLYTKT QDPAKAPNSP DMLEIEFKKG VPVKVTNVGD GTTHSTALEL
260 270 280 290 300
FLYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF
310 320 330 340 350
TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ
360 370 380 390 400
VSVFKGQVYI LGRESPLSLY NEELVSMNVQ GDYEPVDATG FININSLRLK
410
EYHRLQNKVT AK
Length:412
Mass (Da):46,417
Last modified:January 1, 1990 - v1
Checksum:i6F74C7F445EE0D86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26198 mRNA. Translation: AAA30388.1.
BC102474 mRNA. Translation: AAI02475.1.
PIRiA33986. AJBORS.
RefSeqiNP_776317.1. NM_173892.4.
UniGeneiBt.1370.

Genome annotation databases

EnsembliENSBTAT00000027649; ENSBTAP00000027649; ENSBTAG00000020747.
GeneIDi280726.
KEGGibta:280726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26198 mRNA. Translation: AAA30388.1.
BC102474 mRNA. Translation: AAI02475.1.
PIRiA33986. AJBORS.
RefSeqiNP_776317.1. NM_173892.4.
UniGeneiBt.1370.

3D structure databases

ProteinModelPortaliP14568.
SMRiP14568. Positions 4-407.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP14568.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000027649; ENSBTAP00000027649; ENSBTAG00000020747.
GeneIDi280726.
KEGGibta:280726.

Organism-specific databases

CTDi445.

Phylogenomic databases

eggNOGiCOG0137.
GeneTreeiENSGT00390000004524.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiP14568.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG7PVWPB.
TreeFamiTF300736.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.
ReactomeiREACT_208559. Urea cycle.
SABIO-RKP14568.

Miscellaneous databases

NextBioi20804902.

Gene expression databases

ExpressionAtlasiP14568. baseline.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular definition of bovine argininosuccinate synthetase deficiency."
    Dennis J.A., Healy P.J., Beaudet A.L., O'Brien W.E.
    Proc. Natl. Acad. Sci. U.S.A. 86:7947-7951(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Ileum.

Entry informationi

Entry nameiASSY_BOVIN
AccessioniPrimary (citable) accession number: P14568
Secondary accession number(s): Q3T0A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: March 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.