Reviewed, UniProtKB/Swiss-Prot P14568 (ASSY_BOVIN)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	ASS1
Synonyms:	ASS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
Subunit structure	Homotetramer.
Involvement in disease	Defects in ASS1 are the cause of a bovine form of citrullinemia.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Urea cycle
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW urea cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW argininosuccinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Argininosuccinate synthase		PRO_0000148553
Regions
	Nucleotide binding	10 – 18	9	ATP By similarity
	Nucleotide binding	115 – 123	9	ATP By similarity
Sites
	Binding site	36	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	87	1	Citrulline By similarity
	Binding site	92	1	Citrulline By similarity
	Binding site	119	1	Aspartate By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	123	1	Citrulline By similarity
	Binding site	124	1	Aspartate By similarity
	Binding site	127	1	Citrulline By similarity
	Binding site	180	1	Citrulline By similarity
	Binding site	189	1	Citrulline By similarity
	Binding site	270	1	Citrulline By similarity
	Binding site	282	1	Citrulline By similarity
Amino acid modifications
	Modified residue	165	1	N6-acetyllysine By similarity
	Modified residue	352	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14568-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 6F74C7F445EE0D86	FASTA	412	46,417
	10 20 30 40 50 60 MSGKGSVVLA YSGGLDTSCI LVWLKEQGYD VIAYLANIGQ KEDFEEARKK ALKLGAKKVF 70 80 90 100 110 120 IEDISKEFVE EFIWPAIQSS ALYEDRYLLG TSLARPCIAR KQVEIAQREG AKYVSHGATG 130 140 150 160 170 180 KGNDQIRFEL TCYSLAPQIK VIAPWRMPEF YNRFQGRNDL MEYAKQHGIP VPVTPKNPWS 190 200 210 220 230 240 MDENLMHISY EAGILENPKN QAPPGLYTKT QDPAKAPNSP DMLEIEFKKG VPVKVTNVGD 250 260 270 280 290 300 GTTHSTALEL FLYLNEVAGK HGVGRIDIVE NRFIGMKSRG IYETPAGTIL YHAHLDIEAF 310 320 330 340 350 360 TMDREVRKIK QGLGLKFAEL VYTGFWHSPE CEFVRHCIAK SQERVEGKVQ VSVFKGQVYI 370 380 390 400 410 LGRESPLSLY NEELVSMNVQ GDYEPVDATG FININSLRLK EYHRLQNKVT AK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular definition of bovine argininosuccinate synthetase deficiency." Dennis J.A., Healy P.J., Beaudet A.L., O'Brien W.E. Proc. Natl. Acad. Sci. U.S.A. 86:7947-7951(1989) [PubMed: 2813370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Ileum.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26198 mRNA. Translation: AAA30388.1. BC102474 mRNA. Translation: AAI02475.1.
IPI	IPI00713543.
PIR	AJBORS. A33986.
RefSeq	NP_776317.1.
UniGene	Bt.1370
3D structure databases
HSSP	HSSP built from PDB template 1KP2 based on UniProtKB P22767.
SMR	P14568. Positions 4-407.
ModBase	Search...
Protein-protein interaction databases
STRING	P14568.
Proteomic databases
PRIDE	P14568.
Genome annotation databases
Ensembl	ENSBTAT00000027649; ENSBTAP00000027649; ENSBTAG00000020747; Bos taurus. [Genome view]
GeneID	280726.
KEGG	bta:280726.
Organism-specific databases
CTD	280726.
Phylogenomic databases
HOVERGEN	P14568.
OMA	YVFPMFR.
Enzyme and pathway databases
BRENDA	6.3.4.5. 251.
Family and domain databases
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. [Graphical view]
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_BOVIN

Accession

Primary (citable) accession number: P14568
Secondary accession number(s): Q3T0A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	November 3, 2009
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)