Reviewed, UniProtKB/Swiss-Prot P14560 (BLA2_STRCI)

Names and origin

Protein names

Recommended name:
    Beta-lactamase 2
    EC=3.5.2.6
Alternative name(s):
    Penicillinase

Gene names

Name:

blaU

Organism

Streptomyces cacaoi

Taxonomic identifier

1898 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26
	Chain	27 – 314	288	Beta-lactamase 2		PRO_0000017017
Regions
	Region	258 – 260	3	Substrate binding By similarity
Sites
	Active site	96	1	Acyl-ester intermediate

Sequences

	Sequence		Length	Mass (Da)
	P14560-1 [UniParc]. Last modified April 1, 1990. Version 2. Checksum: F895674E3B22ACD3	FASTA	314	34,023
	10 20 30 40 50 60 MLHTRIRRAT LGAVAALSLV PVMACGQESS ADAAEPAGSA PSSSAAAHKP GEVEPYAAEL 70 80 90 100 110 120 KALEDEFDVR LGVYAVDTGS GREVAYRDGE RFPYNSTFKA LECGAVLDKH TDREMDRVVK 130 140 150 160 170 180 YSEDDLVDNS PVTEKHVEDG MTLTALCDAA VRYSDNTAAN LLFETVGGPK GLDKTLEGLG 190 200 210 220 230 240 DHVTRMERVE PFLSRWEPGS KRDTSTPRAF AKDLRAYVLG DVLAEGDRKQ LTTWLRNNTT 250 260 270 280 290 300 GDGLIRAGVR QGWVVGDKTG TGSYYGARND MAVVWRPDGR PLVLNVMVHG HTKDAELDSE 310 LIARATEVVA DRLG

References

[1]	"Transcriptional induction of Streptomyces cacaoi beta-lactamase by a beta-lactam compound." Forsman M., Lindgren L., Haeggstroem B., Jaurin B. Mol. Microbiol. 3:1425-1432(1989) [PubMed: 2559297] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 40057.
[2]	"Two different beta-lactamase genes are present in Streptomyces cacaoi." Magdalena J., Forsman M., Lenzini M.V., Brans A., Dusart J. FEMS Microbiol. Lett. 78:101-105(1992) [PubMed: 1468613] [Abstract] Cited for: COMPARISON OF BLAU AND BLAL.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15708 Genomic DNA. Translation: CAA33739.1.
PIR	S06967.
3D structure databases
HSSP	HSSP built from PDB template 1I2S based on UniProtKB P00808.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 19049.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA2_STRCI

Accession

Primary (citable) accession number: P14560

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	April 1, 1990
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)