P14545 (COX2_LEITA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Cytochrome c oxidase subunit 2 EC=1.9.3.1 Alternative name(s): Cytochrome c oxidase polypeptide II
Encoded on	Mitochondrion
Organism	Leishmania tarentolae (Sauroleishmania tarentolae)
Taxonomic identifier	5689 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmaniinae › Leishmania › lizard Leishmania

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Cofactor	Copper A.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 2 family.
RNA editing	Modified position: not applicable. Some positions are modified by RNA editing via nucleotide insertion. Ref.3

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	RNA editing
Domain	Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Cytochrome c oxidase subunit 2		PRO_0000183616
Regions
	Topological domain	1 – 20	20	Mitochondrial intermembrane Potential
	Transmembrane	21 – 42	22	Helical; Potential
	Topological domain	43 – 60	18	Mitochondrial matrix Potential
	Transmembrane	61 – 86	26	Helical; Potential
	Topological domain	87 – 210	124	Mitochondrial intermembrane Potential
Sites
	Metal binding	157	1	Copper A Probable
	Metal binding	192	1	Copper A Probable
	Metal binding	196	1	Copper A Probable
	Metal binding	200	1	Copper A Probable

Sequences

	Sequence		Length	Mass (Da)
	P14545 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: B3A0544E258047C2	FASTA	210	23,977
	10 20 30 40 50 60 MAFILSFWMI FLLDSVIVLL SFVCFVCVWI CALLFSTVLL VSKLNNIYCT WDFTASKFID 70 80 90 100 110 120 VYWFTIGGMF SLGLLLRLCL LLYFGHLNFV SFDLCKVVGF QWYWVYFIFG ETTIFSNLIL 130 140 150 160 170 180 ESDYMIGDLR LLQCNHVLTL LSLVIYKLWL SAVDVIHSFA ISSLGVKVDC IPGRCNEIVL 190 200 210 FSSNNATVYG QCSELCGVLH GFMPIVICFI

References

[1]	"Sequences of six genes and several open reading frames in the kinetoplast maxicircle DNA of Leishmania tarentolae." de la Cruz V.F., Neckelmann N., Simpson L. J. Biol. Chem. 259:15136-15147(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Mapping and 5' end determination of kinetoplast maxicircle gene transcripts from Leishmania tarentolae." Simpson A.M., Necklemann N., la Cruz V.F., Muhich M.L., Simpson L. Nucleic Acids Res. 13:5977-5993(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Internal frameshifts within the mitochondrial genes for cytochrome oxidase subunit II and maxicircle unidentified reading frame 3 of Leishmania tarentolae are corrected by RNA editing: evidence for translation of the edited cytochrome oxidase subunit II mRNA." Shaw J., Campbell D., Simpson L. Proc. Natl. Acad. Sci. U.S.A. 86:6220-6224(1989) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L07544 mRNA. Translation: AAA31876.1. M10126 Genomic DNA. No translation available.
PIR	I22848.
3D structure databases
ProteinModelPortal	P14545.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.40.420. 1 hit.
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR002429. Cyt_c_oxidase_su2_C. [Graphical view]
Pfam	PF00116. COX2. 1 hit. [Graphical view]
SUPFAM	SSF49503. Cupredoxin. 1 hit.
PROSITE	PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX2_LEITA

Accession

Primary (citable) accession number: P14545

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)