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Protein

Fructose-bisphosphate aldolase

Gene

FBA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PGI1)
  3. ATP-dependent 6-phosphofructokinase subunit beta (PFK2), ATP-dependent 6-phosphofructokinase subunit alpha (PFK1)
  4. Fructose-bisphosphate aldolase (FBA1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63Glyceraldehyde 3-phosphateBy similarity1
Active sitei110Proton donorBy similarity1
Metal bindingi111Zinc 1; catalyticBy similarity1
Metal bindingi145Zinc 2By similarity1
Metal bindingi175Zinc 2By similarity1
Metal bindingi227Zinc 1; catalyticBy similarity1
Binding sitei228Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi265Zinc 1; catalyticBy similarity1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: SGD
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • gluconeogenesis Source: SGD
  • glycolytic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YKL060C-MONOMER.
BRENDAi4.1.2.13. 984.
SABIO-RKP14540.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:FBA1
Ordered Locus Names:YKL060C
ORF Names:YKL320
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL060C.
SGDiS000001543. FBA1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • fungal-type cell wall Source: GO_Central
  • mitochondrion Source: SGD
  • plasma membrane Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001787622 – 359Fructose-bisphosphate aldolaseAdd BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphothreonineCombined sources1
Cross-linki27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei56PhosphoserineCombined sources1
Modified residuei63PhosphoserineCombined sources1
Cross-linki73Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei76PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Cross-linki85Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei96PhosphoserineCombined sources1
Modified residuei147PhosphoserineCombined sources1
Modified residuei150PhosphothreonineCombined sources1
Modified residuei179PhosphothreonineCombined sources1
Modified residuei268PhosphoserineCombined sources1
Modified residuei290PhosphothreonineCombined sources1
Cross-linki308Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei310PhosphotyrosineCombined sources1
Modified residuei313PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP14540.
PRIDEiP14540.
TopDownProteomicsiP14540.

2D gel databases

COMPLUYEAST-2DPAGEP14540.
SWISS-2DPAGEP14540.
UCD-2DPAGEP14540.

PTM databases

iPTMnetiP14540.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34073. 95 interactors.
DIPiDIP-4702N.
IntActiP14540. 40 interactors.
MINTiMINT-8285308.

Structurei

3D structure databases

ProteinModelPortaliP14540.
SMRiP14540.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni266 – 268Dihydroxyacetone phosphate bindingBy similarity3
Regioni287 – 290Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000227794.
InParanoidiP14540.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG092C385G.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVEQILKRK TGVIVGEDVH NLFTYAKEHK FAIPAINVTS SSTAVAALEA
60 70 80 90 100
ARDSKSPIIL QTSNGGAAYF AGKGISNEGQ NASIKGAIAA AHYIRSIAPA
110 120 130 140 150
YGIPVVLHSD HCAKKLLPWF DGMLEADEAY FKEHGEPLFS SHMLDLSEET
160 170 180 190 200
DEENISTCVK YFKRMAAMDQ WLEMEIGITG GEEDGVNNEN ADKEDLYTKP
210 220 230 240 250
EQVYNVYKAL HPISPNFSIA AAFGNCHGLY AGDIALRPEI LAEHQKYTRE
260 270 280 290 300
QVGCKEEKPL FLVFHGGSGS TVQEFHTGID NGVVKVNLDT DCQYAYLTGI
310 320 330 340 350
RDYVLNKKDY IMSPVGNPEG PEKPNKKFFD PRVWVREGEK TMGAKITKSL

ETFRTTNTL
Length:359
Mass (Da):39,621
Last modified:January 23, 2007 - v3
Checksum:iC67E61BA5C7E8E4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15003 Genomic DNA. Translation: CAA33111.1.
Z28060 Genomic DNA. Translation: CAA81897.1.
X75781 Genomic DNA. Translation: CAA53412.1.
M32026 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09097.1.
PIRiS07855. ADBY2.
RefSeqiNP_012863.1. NM_001179626.1.

Genome annotation databases

EnsemblFungiiYKL060C; YKL060C; YKL060C.
GeneIDi853805.
KEGGisce:YKL060C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15003 Genomic DNA. Translation: CAA33111.1.
Z28060 Genomic DNA. Translation: CAA81897.1.
X75781 Genomic DNA. Translation: CAA53412.1.
M32026 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09097.1.
PIRiS07855. ADBY2.
RefSeqiNP_012863.1. NM_001179626.1.

3D structure databases

ProteinModelPortaliP14540.
SMRiP14540.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34073. 95 interactors.
DIPiDIP-4702N.
IntActiP14540. 40 interactors.
MINTiMINT-8285308.

PTM databases

iPTMnetiP14540.

2D gel databases

COMPLUYEAST-2DPAGEP14540.
SWISS-2DPAGEP14540.
UCD-2DPAGEP14540.

Proteomic databases

MaxQBiP14540.
PRIDEiP14540.
TopDownProteomicsiP14540.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL060C; YKL060C; YKL060C.
GeneIDi853805.
KEGGisce:YKL060C.

Organism-specific databases

EuPathDBiFungiDB:YKL060C.
SGDiS000001543. FBA1.

Phylogenomic databases

HOGENOMiHOG000227794.
InParanoidiP14540.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG092C385G.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciYEAST:YKL060C-MONOMER.
BRENDAi4.1.2.13. 984.
SABIO-RKP14540.

Miscellaneous databases

PROiP14540.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_YEAST
AccessioniPrimary (citable) accession number: P14540
Secondary accession number(s): D6VXM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 167 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1020000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.