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P14540

- ALF_YEAST

UniProt

P14540 - ALF_YEAST

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Protein
Fructose-bisphosphate aldolase
Gene
FBA1, YKL060C, YKL320
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis By similarity.

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631Glyceraldehyde 3-phosphate By similarity
Active sitei110 – 1101Proton donor By similarity
Metal bindingi111 – 1111Zinc 1; catalytic By similarity
Metal bindingi145 – 1451Zinc 2 By similarity
Metal bindingi175 – 1751Zinc 2 By similarity
Metal bindingi227 – 2271Zinc 1; catalytic By similarity
Binding sitei228 – 2281Dihydroxyacetone phosphate; via amide nitrogen By similarity
Metal bindingi265 – 2651Zinc 1; catalytic By similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: SGD
  2. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. gluconeogenesis Source: SGD
  2. glycolytic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YKL060C-MONOMER.
SABIO-RKP14540.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:FBA1
Ordered Locus Names:YKL060C
ORF Names:YKL320
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

SGDiS000001543. FBA1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: SGD
  2. mitochondrion Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 359358Fructose-bisphosphate aldolase
PRO_0000178762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111Phosphothreonine2 Publications
Modified residuei56 – 561Phosphoserine1 Publication
Modified residuei63 – 631Phosphoserine1 Publication
Modified residuei76 – 761Phosphoserine2 Publications
Modified residuei83 – 831Phosphoserine1 Publication
Modified residuei96 – 961Phosphoserine1 Publication
Modified residuei147 – 1471Phosphoserine2 Publications
Modified residuei150 – 1501Phosphothreonine2 Publications
Modified residuei179 – 1791Phosphothreonine1 Publication
Modified residuei268 – 2681Phosphoserine1 Publication
Modified residuei290 – 2901Phosphothreonine2 Publications
Modified residuei310 – 3101Phosphotyrosine1 Publication
Modified residuei313 – 3131Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP14540.
PaxDbiP14540.
PeptideAtlasiP14540.

2D gel databases

COMPLUYEAST-2DPAGEP14540.
SWISS-2DPAGEP14540.
UCD-2DPAGEP14540.

Expressioni

Gene expression databases

GenevestigatoriP14540.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34073. 96 interactions.
DIPiDIP-4702N.
IntActiP14540. 36 interactions.
MINTiMINT-8285308.
STRINGi4932.YKL060C.

Structurei

3D structure databases

ProteinModelPortaliP14540.
SMRiP14540. Positions 10-359.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni266 – 2683Dihydroxyacetone phosphate binding By similarity
Regioni287 – 2904Dihydroxyacetone phosphate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
HOGENOMiHOG000227794.
KOiK01624.
OMAiCNDLHSA.
OrthoDBiEOG7HTHSN.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14540-1 [UniParc]FASTAAdd to Basket

« Hide

MGVEQILKRK TGVIVGEDVH NLFTYAKEHK FAIPAINVTS SSTAVAALEA    50
ARDSKSPIIL QTSNGGAAYF AGKGISNEGQ NASIKGAIAA AHYIRSIAPA 100
YGIPVVLHSD HCAKKLLPWF DGMLEADEAY FKEHGEPLFS SHMLDLSEET 150
DEENISTCVK YFKRMAAMDQ WLEMEIGITG GEEDGVNNEN ADKEDLYTKP 200
EQVYNVYKAL HPISPNFSIA AAFGNCHGLY AGDIALRPEI LAEHQKYTRE 250
QVGCKEEKPL FLVFHGGSGS TVQEFHTGID NGVVKVNLDT DCQYAYLTGI 300
RDYVLNKKDY IMSPVGNPEG PEKPNKKFFD PRVWVREGEK TMGAKITKSL 350
ETFRTTNTL 359
Length:359
Mass (Da):39,621
Last modified:January 23, 2007 - v3
Checksum:iC67E61BA5C7E8E4C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15003 Genomic DNA. Translation: CAA33111.1.
Z28060 Genomic DNA. Translation: CAA81897.1.
X75781 Genomic DNA. Translation: CAA53412.1.
M32026 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09097.1.
PIRiS07855. ADBY2.
RefSeqiNP_012863.1. NM_001179626.1.

Genome annotation databases

EnsemblFungiiYKL060C; YKL060C; YKL060C.
GeneIDi853805.
KEGGisce:YKL060C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15003 Genomic DNA. Translation: CAA33111.1 .
Z28060 Genomic DNA. Translation: CAA81897.1 .
X75781 Genomic DNA. Translation: CAA53412.1 .
M32026 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09097.1 .
PIRi S07855. ADBY2.
RefSeqi NP_012863.1. NM_001179626.1.

3D structure databases

ProteinModelPortali P14540.
SMRi P14540. Positions 10-359.
ModBasei Search...

Protein-protein interaction databases

BioGridi 34073. 96 interactions.
DIPi DIP-4702N.
IntActi P14540. 36 interactions.
MINTi MINT-8285308.
STRINGi 4932.YKL060C.

2D gel databases

COMPLUYEAST-2DPAGE P14540.
SWISS-2DPAGE P14540.
UCD-2DPAGE P14540.

Proteomic databases

MaxQBi P14540.
PaxDbi P14540.
PeptideAtlasi P14540.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL060C ; YKL060C ; YKL060C .
GeneIDi 853805.
KEGGi sce:YKL060C.

Organism-specific databases

SGDi S000001543. FBA1.

Phylogenomic databases

eggNOGi COG0191.
HOGENOMi HOG000227794.
KOi K01624.
OMAi CNDLHSA.
OrthoDBi EOG7HTHSN.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00183 .
BioCyci YEAST:YKL060C-MONOMER.
SABIO-RK P14540.

Miscellaneous databases

NextBioi 974960.
PROi P14540.

Gene expression databases

Genevestigatori P14540.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view ]
Pfami PF01116. F_bP_aldolase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsi TIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEi PS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, primary structure and disruption of the structural gene of aldolase from Saccharomyces cerevisiae."
    Schwelberger H.G., Kohlwein S.D., Paltauf F.
    Eur. J. Biochem. 180:301-308(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Sequence of a 28.6 kb region of yeast chromosome XI includes the FBA1 and TOA2 genes, an open reading frame (ORF) similar to a translationally controlled tumour protein, one ORF containing motifs also found in plant storage proteins and 13 ORFs with weak or no homology to known proteins."
    Rasmussen S.W.
    Yeast 10:S63-S68(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Cloning of Saccharomyces cerevisiae promoters using a probe vector based on phleomycin resistance."
    Gatignol A., Dassain M., Tiraby G.
    Gene 91:35-41(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96.
  6. Jack R.S.
    Thesis (1973), University of Cambridge, United Kingdom
    Cited for: PRELIMINARY PROTEIN SEQUENCE OF 2-40.
  7. Cited for: PROTEIN SEQUENCE OF 333-339.
    Strain: ATCC 204508 / S288c.
  8. "Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae."
    Norbeck J., Blomberg A.
    FEMS Microbiol. Lett. 137:1-8(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
    Strain: ATCC 38531 / Y41.
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11; THR-150; TYR-310 AND SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-11; SER-147; THR-179 AND SER-268, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76; SER-96; SER-147; THR-150; THR-290 AND SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-56; SER-63; SER-76; SER-83; THR-290 AND SER-313, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALF_YEAST
AccessioniPrimary (citable) accession number: P14540
Secondary accession number(s): D6VXM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1020000 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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