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Protein

Fructose-bisphosphate aldolase

Gene

FBA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PGI1)
  3. ATP-dependent 6-phosphofructokinase subunit beta (PFK2), ATP-dependent 6-phosphofructokinase subunit alpha (PFK1)
  4. Fructose-bisphosphate aldolase (FBA1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631Glyceraldehyde 3-phosphateBy similarity
Active sitei110 – 1101Proton donorBy similarity
Metal bindingi111 – 1111Zinc 1; catalyticBy similarity
Metal bindingi145 – 1451Zinc 2By similarity
Metal bindingi175 – 1751Zinc 2By similarity
Metal bindingi227 – 2271Zinc 1; catalyticBy similarity
Binding sitei228 – 2281Dihydroxyacetone phosphate; via amide nitrogenBy similarity
Metal bindingi265 – 2651Zinc 1; catalyticBy similarity

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: SGD
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • gluconeogenesis Source: SGD
  • glycolytic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YKL060C-MONOMER.
BRENDAi4.1.2.13. 984.
SABIO-RKP14540.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:FBA1
Ordered Locus Names:YKL060C
ORF Names:YKL320
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL060C.
SGDiS000001543. FBA1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • fungal-type cell wall Source: GO_Central
  • mitochondrion Source: SGD
  • plasma membrane Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 359358Fructose-bisphosphate aldolasePRO_0000178762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphothreonineCombined sources
Cross-linki27 – 27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei56 – 561PhosphoserineCombined sources
Modified residuei63 – 631PhosphoserineCombined sources
Cross-linki73 – 73Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei76 – 761PhosphoserineCombined sources
Modified residuei83 – 831PhosphoserineCombined sources
Cross-linki85 – 85Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei96 – 961PhosphoserineCombined sources
Modified residuei147 – 1471PhosphoserineCombined sources
Modified residuei150 – 1501PhosphothreonineCombined sources
Modified residuei179 – 1791PhosphothreonineCombined sources
Modified residuei268 – 2681PhosphoserineCombined sources
Modified residuei290 – 2901PhosphothreonineCombined sources
Cross-linki308 – 308Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei310 – 3101PhosphotyrosineCombined sources
Modified residuei313 – 3131PhosphoserineCombined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP14540.
TopDownProteomicsiP14540.

2D gel databases

COMPLUYEAST-2DPAGEP14540.
SWISS-2DPAGEP14540.
UCD-2DPAGEP14540.

PTM databases

iPTMnetiP14540.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi34073. 95 interactions.
DIPiDIP-4702N.
IntActiP14540. 40 interactions.
MINTiMINT-8285308.

Structurei

3D structure databases

ProteinModelPortaliP14540.
SMRiP14540. Positions 10-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni266 – 2683Dihydroxyacetone phosphate bindingBy similarity
Regioni287 – 2904Dihydroxyacetone phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000227794.
InParanoidiP14540.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG092C385G.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVEQILKRK TGVIVGEDVH NLFTYAKEHK FAIPAINVTS SSTAVAALEA
60 70 80 90 100
ARDSKSPIIL QTSNGGAAYF AGKGISNEGQ NASIKGAIAA AHYIRSIAPA
110 120 130 140 150
YGIPVVLHSD HCAKKLLPWF DGMLEADEAY FKEHGEPLFS SHMLDLSEET
160 170 180 190 200
DEENISTCVK YFKRMAAMDQ WLEMEIGITG GEEDGVNNEN ADKEDLYTKP
210 220 230 240 250
EQVYNVYKAL HPISPNFSIA AAFGNCHGLY AGDIALRPEI LAEHQKYTRE
260 270 280 290 300
QVGCKEEKPL FLVFHGGSGS TVQEFHTGID NGVVKVNLDT DCQYAYLTGI
310 320 330 340 350
RDYVLNKKDY IMSPVGNPEG PEKPNKKFFD PRVWVREGEK TMGAKITKSL

ETFRTTNTL
Length:359
Mass (Da):39,621
Last modified:January 23, 2007 - v3
Checksum:iC67E61BA5C7E8E4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15003 Genomic DNA. Translation: CAA33111.1.
Z28060 Genomic DNA. Translation: CAA81897.1.
X75781 Genomic DNA. Translation: CAA53412.1.
M32026 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09097.1.
PIRiS07855. ADBY2.
RefSeqiNP_012863.1. NM_001179626.1.

Genome annotation databases

EnsemblFungiiYKL060C; YKL060C; YKL060C.
GeneIDi853805.
KEGGisce:YKL060C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15003 Genomic DNA. Translation: CAA33111.1.
Z28060 Genomic DNA. Translation: CAA81897.1.
X75781 Genomic DNA. Translation: CAA53412.1.
M32026 Genomic DNA. No translation available.
BK006944 Genomic DNA. Translation: DAA09097.1.
PIRiS07855. ADBY2.
RefSeqiNP_012863.1. NM_001179626.1.

3D structure databases

ProteinModelPortaliP14540.
SMRiP14540. Positions 10-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34073. 95 interactions.
DIPiDIP-4702N.
IntActiP14540. 40 interactions.
MINTiMINT-8285308.

PTM databases

iPTMnetiP14540.

2D gel databases

COMPLUYEAST-2DPAGEP14540.
SWISS-2DPAGEP14540.
UCD-2DPAGEP14540.

Proteomic databases

MaxQBiP14540.
TopDownProteomicsiP14540.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL060C; YKL060C; YKL060C.
GeneIDi853805.
KEGGisce:YKL060C.

Organism-specific databases

EuPathDBiFungiDB:YKL060C.
SGDiS000001543. FBA1.

Phylogenomic databases

HOGENOMiHOG000227794.
InParanoidiP14540.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG092C385G.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciYEAST:YKL060C-MONOMER.
BRENDAi4.1.2.13. 984.
SABIO-RKP14540.

Miscellaneous databases

PROiP14540.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_YEAST
AccessioniPrimary (citable) accession number: P14540
Secondary accession number(s): D6VXM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 164 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1020000 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.