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Protein

Cytochrome c551 peroxidase

Gene

ccpA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the peroxidative oxidation of azurin and cytochrome c551. Likely to provide protection against toxic peroxides.

Catalytic activityi

2 ferrocytochrome c + H2O2 = 2 ferricytochrome c + 2 H2O.

Cofactori

hemeNote: Binds 2 heme groups. Heme 1 is low-potential (-330 mV) with 2 His axial ligands and functions in the peroxidase reaction, while heme 2 is high potential (+320 mV) with His and Met axial ligands and functions to feed electrons from electron-shuttle proteins such as cytochrome c and azurin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74Heme 1 (covalent)1
Binding sitei77Heme 1 (covalent)1
Metal bindingi78Iron (heme 1 axial ligand)1
Binding sitei220Heme 2 (covalent)1
Binding sitei223Heme 2 (covalent)1
Metal bindingi224Iron (heme 2 axial ligand)1
Metal bindingi284Iron (heme 1 axial ligand)1
Metal bindingi298Iron (heme 2 axial ligand)1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17891.
BRENDAi1.11.1.5. 5087.

Protein family/group databases

PeroxiBasei3543. PaerDiHCcP.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c551 peroxidase (EC:1.11.1.5)
Short name:
CCP
Short name:
Cytochrome c peroxidase
Gene namesi
Name:ccpA
Ordered Locus Names:PA4587
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4587.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 231 PublicationAdd BLAST23
ChainiPRO_000000659824 – 346Cytochrome c551 peroxidase1 PublicationAdd BLAST323

Post-translational modificationi

Binds 2 heme groups per subunit.

Proteomic databases

PaxDbiP14532.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4587.

Structurei

Secondary structure

1346
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi25 – 33Combined sources9
Helixi51 – 61Combined sources11
Helixi64 – 66Combined sources3
Beta strandi67 – 70Combined sources4
Helixi74 – 77Combined sources4
Helixi80 – 82Combined sources3
Beta strandi88 – 90Combined sources3
Helixi94 – 96Combined sources3
Helixi109 – 111Combined sources3
Beta strandi112 – 116Combined sources5
Beta strandi121 – 123Combined sources3
Helixi124 – 127Combined sources4
Helixi129 – 133Combined sources5
Turni135 – 138Combined sources4
Helixi142 – 150Combined sources9
Helixi153 – 162Combined sources10
Beta strandi167 – 171Combined sources5
Helixi172 – 183Combined sources12
Helixi192 – 197Combined sources6
Helixi201 – 203Combined sources3
Helixi206 – 217Combined sources12
Helixi220 – 222Combined sources3
Turni227 – 229Combined sources3
Beta strandi230 – 232Combined sources3
Beta strandi234 – 236Combined sources3
Beta strandi239 – 241Combined sources3
Turni245 – 247Combined sources3
Turni253 – 255Combined sources3
Helixi256 – 259Combined sources4
Helixi262 – 264Combined sources3
Beta strandi267 – 269Combined sources3
Helixi276 – 278Combined sources3
Turni283 – 286Combined sources4
Helixi291 – 303Combined sources13
Helixi309 – 320Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EB7X-ray2.40A24-346[»]
2VHDX-ray2.30A/B24-346[»]
ProteinModelPortaliP14532.
SMRiP14532.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14532.

Family & Domainsi

Domaini

Organized into two domains, each containing a covalent c-heme in a structure reminiscent of class 1 cytochromes c. The domains are related by a quasi-twofold axis. The domain interface contains a calcium-binding site with an unusual set of ligands.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CA0. Bacteria.
COG1858. LUCA.
HOGENOMiHOG000173945.
InParanoidiP14532.
KOiK00428.
OMAiYKFANVG.
PhylomeDBiP14532.

Family and domain databases

Gene3Di1.10.760.10. 2 hits.
InterProiIPR009056. Cyt_c-like_dom.
IPR004852. Di-haem_cyt_c_peroxidsae.
IPR026259. MauG/Cytc_peroxidase.
[Graphical view]
PfamiPF03150. CCP_MauG. 1 hit.
PF00034. Cytochrom_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000294. Cytochrome-c_peroxidase. 1 hit.
SUPFAMiSSF46626. SSF46626. 2 hits.
PROSITEiPS51007. CYTC. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSSQLLPLG SLLLSFATPL AQADALHDQA SALFKPIPEQ VTELRGQPIS
60 70 80 90 100
EQQRELGKKL FFDPRLSRSH VLSCNTCHNV GTGGADNVPT SVGHGWQKGP
110 120 130 140 150
RNSPTVFNAV FNAAQFWDGR AKDLGEQAKG PIQNSVEMHS TPQLVEQTLG
160 170 180 190 200
SIPEYVDAFR KAFPKAGKPV SFDNMALAIE AYEATLVTPD SPFDLYLKGD
210 220 230 240 250
DKALDAQQKK GLKAFMDSGC SACHNGINLG GQAYFPFGLV KKPDASVLPS
260 270 280 290 300
GDKGRFAVTK TQSDEYVFRA APLRNVALTA PYFHSGQVWE LKDAVAIMGN
310 320 330 340
AQLGKQLAPD DVENIVAFLH SLSGKQPRVE YPLLPASTET TPRPAE
Length:346
Mass (Da):37,403
Last modified:November 1, 1997 - v2
Checksum:i8B402E5BA149EA44
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86D → G (PubMed:8543038).Curated1
Sequence conflicti93Missing (PubMed:8543038).Curated1
Sequence conflicti96W → G (PubMed:8543038).Curated1
Sequence conflicti102 – 120Missing (PubMed:8543038).CuratedAdd BLAST19
Sequence conflicti137E → Q (PubMed:8543038).Curated1
Sequence conflicti175M → V (PubMed:10984043).Curated1
Sequence conflicti228Missing (PubMed:8543038).Curated1
Sequence conflicti286 – 287GQ → QG (PubMed:8543038).Curated2
Sequence conflicti290E → Q (PubMed:8543038).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti81G → D.1
Natural varianti83G → D.1
Natural varianti152I → V.1
Natural varianti164P → A.1
Natural varianti198K → I.1
Natural varianti334L → W.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23766 Genomic DNA. Translation: AAC43378.1.
AE004091 Genomic DNA. Translation: AAG07975.1.
PIRiI53515.
RefSeqiNP_253277.1. NC_002516.2.
WP_003094815.1. NZ_ASJY01000737.1.

Genome annotation databases

EnsemblBacteriaiAAG07975; AAG07975; PA4587.
GeneIDi881021.
KEGGipae:PA4587.
PATRICi19843919. VBIPseAer58763_4801.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23766 Genomic DNA. Translation: AAC43378.1.
AE004091 Genomic DNA. Translation: AAG07975.1.
PIRiI53515.
RefSeqiNP_253277.1. NC_002516.2.
WP_003094815.1. NZ_ASJY01000737.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EB7X-ray2.40A24-346[»]
2VHDX-ray2.30A/B24-346[»]
ProteinModelPortaliP14532.
SMRiP14532.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4587.

Protein family/group databases

PeroxiBasei3543. PaerDiHCcP.

Proteomic databases

PaxDbiP14532.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG07975; AAG07975; PA4587.
GeneIDi881021.
KEGGipae:PA4587.
PATRICi19843919. VBIPseAer58763_4801.

Organism-specific databases

PseudoCAPiPA4587.

Phylogenomic databases

eggNOGiENOG4105CA0. Bacteria.
COG1858. LUCA.
HOGENOMiHOG000173945.
InParanoidiP14532.
KOiK00428.
OMAiYKFANVG.
PhylomeDBiP14532.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-17891.
BRENDAi1.11.1.5. 5087.

Miscellaneous databases

EvolutionaryTraceiP14532.

Family and domain databases

Gene3Di1.10.760.10. 2 hits.
InterProiIPR009056. Cyt_c-like_dom.
IPR004852. Di-haem_cyt_c_peroxidsae.
IPR026259. MauG/Cytc_peroxidase.
[Graphical view]
PfamiPF03150. CCP_MauG. 1 hit.
PF00034. Cytochrom_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000294. Cytochrome-c_peroxidase. 1 hit.
SUPFAMiSSF46626. SSF46626. 2 hits.
PROSITEiPS51007. CYTC. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCCPR_PSEAE
AccessioniPrimary (citable) accession number: P14532
Secondary accession number(s): Q51369
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.