P14518 (BROM2_ANACO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Stem bromelain EC=3.4.22.32
Organism	Ananas comosus (Pineapple) (Ananas ananas)
Taxonomic identifier	4615 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Bromeliaceae › Ananas

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Broad specificity for cleavage of proteins, but strong preference for Z-Arg-Arg-|-NHMec among small molecule substrates.
Miscellaneous	The geometry and the reactivity of the catalytic site are different from those of other cysteine proteinases.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Stem bromelain		PRO_0000050555
Sites
	Active site	26	1	By similarity
	Active site	158	1	By similarity
Amino acid modifications
	Glycosylation	117	1	N-linked (GlcNAc...)		CAR_000134
	Disulfide bond	23 ↔ 63		By similarity
	Disulfide bond	57 ↔ 96		By similarity
	Disulfide bond	152 ↔ 199		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14518 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 94EBADB72AAFA556	FASTA	212	22,831
	10 20 30 40 50 60 AVPQSIDWRD YGAVTSVKNQ NPCGACWAFA AIATVESIYK IKKGILEPLS EQQVLDCAKG 70 80 90 100 110 120 YGCKGGWEFR AFEFIISNKG VASGAIYPYK AAKGTCKTDG VPNSAYITGY ARVPRNNESS 130 140 150 160 170 180 MMYAVSKQPI TVAVDANANF QYYKSGVFNG PCGTSLNHAV TAIGYGQDSI IYPKKWGAKW 190 200 210 GEAGYIRMAR DVSSSSGICG IAIDPLYPTL EE

References

[1]	"Stem bromelain: amino acid sequence and implications for weak binding of cystatin." Ritonja A., Rowan A.D., Buttle D.J., Rawlings N.D., Turk V., Barrett A.J. FEBS Lett. 247:419-424(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"A 500-MHz 1H-NMR study on the N-linked carbohydrate chain of bromelain. 1H-NMR structural-reporter-groups of fucose alpha(1-3)-linked to asparagine-bound N-acetylglucosamine." van Kuik J.A., Hoffmann R.A., Mutsaers J.H.G.M., van Halbeek H., Kamerling J.P., Vliegenthart J.F.G. Glycoconj. J. 3:27-34(1986) Cited for: STRUCTURE OF CARBOHYDRATE.

Cross-references

Sequence databases

PIR

S03964.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W0Q	model	-	B	2-211	[»]

ProteinModelPortal

P14518.

SMR

P14518. Positions 2-210.

ModBase

Search...

Protein family/group databases

Allergome

3076. Ana c 2.0101.

MEROPS

C01.005.

PTM databases

GlycoSuiteDB

P14518.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

3.4.22.32. 333.

Family and domain databases

InterPro

IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
[Graphical view]

PANTHER

PTHR12411. PTHR12411. 1 hit.

Pfam

PF00112. Peptidase_C1. 1 hit.
[Graphical view]

PRINTS

PR00705. PAPAIN.

SMART

SM00645. Pept_C1. 1 hit.
[Graphical view]

PROSITE

PS00640. THIOL_PROTEASE_ASN. False negative.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

BROM2_ANACO

Accession

Primary (citable) accession number: P14518

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program