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P14489

- BLO10_PSEAI

UniProt

P14489 - BLO10_PSEAI

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Protein

Beta-lactamase OXA-10

Gene

bla

Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes both carbenicillin and oxacillin.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Acyl-ester intermediate

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC
  2. penicillin binding Source: InterPro

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXA-10 (EC:3.5.2.6)
Alternative name(s):
Beta-lactamase PSE-2
Gene namesi
Name:bla
Synonyms:oxa10, pse2
Encoded oniPlasmid pMON2340 Publication
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 266246Beta-lactamase OXA-10PRO_0000017030Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 51
Modified residuei70 – 701N6-carboxylysine1 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Structurei

Secondary structure

1
266
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 243Combined sources
Helixi26 – 283Combined sources
Helixi29 – 335Combined sources
Turni34 – 363Combined sources
Beta strandi39 – 479Combined sources
Beta strandi50 – 545Combined sources
Helixi56 – 594Combined sources
Helixi66 – 694Combined sources
Helixi70 – 7910Combined sources
Beta strandi81 – 833Combined sources
Helixi100 – 1023Combined sources
Helixi108 – 1136Combined sources
Helixi117 – 12711Combined sources
Helixi129 – 13810Combined sources
Turni150 – 1523Combined sources
Helixi153 – 1564Combined sources
Helixi163 – 17412Combined sources
Beta strandi178 – 1803Combined sources
Helixi182 – 19110Combined sources
Beta strandi193 – 1964Combined sources
Beta strandi198 – 20811Combined sources
Beta strandi214 – 2163Combined sources
Beta strandi218 – 22811Combined sources
Beta strandi231 – 24212Combined sources
Helixi244 – 2485Combined sources
Helixi249 – 26012Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E3UX-ray1.66A/B/C/D21-266[»]
1E4DX-ray1.80A/B/C/D21-266[»]
1EWZX-ray2.40A/B/C/D21-266[»]
1FOFX-ray2.00A/B20-265[»]
1K4EX-ray2.00A/B20-266[»]
1K4FX-ray1.60A/B20-266[»]
1K54X-ray1.70A/B/C/D21-266[»]
1K55X-ray1.39A/B/C/D21-266[»]
1K56X-ray1.70A/B/C/D21-266[»]
1K57X-ray1.90A/B/C/D21-266[»]
1K6RX-ray2.30A/B20-266[»]
1K6SX-ray2.03A/B20-266[»]
2HP5X-ray2.70A/B/C/D20-266[»]
2HP6X-ray2.20A/B20-266[»]
2HP9X-ray2.50A/B20-266[»]
2HPBX-ray2.05A/B20-266[»]
2RL3X-ray1.90A/B20-266[»]
2WGIX-ray2.85A/B21-266[»]
2WGVX-ray1.80A/B20-266[»]
2WGWX-ray1.80A/B20-266[»]
2WKHX-ray1.79A/B20-266[»]
2WKIX-ray2.10A/B20-266[»]
2X01X-ray1.90A/B20-266[»]
2X02X-ray1.35A/B20-266[»]
3LCEX-ray2.00A/B/C/D21-266[»]
ProteinModelPortaliP14489.
SMRiP14489. Positions 21-266.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14489.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 2073Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-D beta-lactamase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14489-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTFAAYVII ACLSSTALAG SITENTSWNK EFSAEAVNGV FVLCKSSSKS
60 70 80 90 100
CATNDLARAS KEYLPASTFK IPNAIIGLET GVIKNEHQVF KWDGKPRAMK
110 120 130 140 150
QWERDLTLRG AIQVSAVPVF QQIAREVGEV RMQKYLKKFS YGNQNISGGI
160 170 180 190 200
DKFWLEGQLR ISAVNQVEFL ESLYLNKLSA SKENQLIVKE ALVTEAAPEY
210 220 230 240 250
LVHSKTGFSG VGTESNPGVA WWVGWVEKET EVYFFAFNMD IDNESKLPLR
260
KSIPTKIMES EGIIGG
Length:266
Mass (Da):29,507
Last modified:January 1, 1990 - v1
Checksum:i9ABFF429D028F240
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37105 Genomic DNA. Translation: AAB60534.1.
J03427 Genomic DNA. Translation: AAA25648.1.
PIRiS06462.
RefSeqiYP_008532606.1. NC_022344.1.

Genome annotation databases

GeneIDi16834474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37105 Genomic DNA. Translation: AAB60534.1 .
J03427 Genomic DNA. Translation: AAA25648.1 .
PIRi S06462.
RefSeqi YP_008532606.1. NC_022344.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E3U X-ray 1.66 A/B/C/D 21-266 [» ]
1E4D X-ray 1.80 A/B/C/D 21-266 [» ]
1EWZ X-ray 2.40 A/B/C/D 21-266 [» ]
1FOF X-ray 2.00 A/B 20-265 [» ]
1K4E X-ray 2.00 A/B 20-266 [» ]
1K4F X-ray 1.60 A/B 20-266 [» ]
1K54 X-ray 1.70 A/B/C/D 21-266 [» ]
1K55 X-ray 1.39 A/B/C/D 21-266 [» ]
1K56 X-ray 1.70 A/B/C/D 21-266 [» ]
1K57 X-ray 1.90 A/B/C/D 21-266 [» ]
1K6R X-ray 2.30 A/B 20-266 [» ]
1K6S X-ray 2.03 A/B 20-266 [» ]
2HP5 X-ray 2.70 A/B/C/D 20-266 [» ]
2HP6 X-ray 2.20 A/B 20-266 [» ]
2HP9 X-ray 2.50 A/B 20-266 [» ]
2HPB X-ray 2.05 A/B 20-266 [» ]
2RL3 X-ray 1.90 A/B 20-266 [» ]
2WGI X-ray 2.85 A/B 21-266 [» ]
2WGV X-ray 1.80 A/B 20-266 [» ]
2WGW X-ray 1.80 A/B 20-266 [» ]
2WKH X-ray 1.79 A/B 20-266 [» ]
2WKI X-ray 2.10 A/B 20-266 [» ]
2X01 X-ray 1.90 A/B 20-266 [» ]
2X02 X-ray 1.35 A/B 20-266 [» ]
3LCE X-ray 2.00 A/B/C/D 21-266 [» ]
ProteinModelPortali P14489.
SMRi P14489. Positions 21-266.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P14489.
ChEMBLi CHEMBL5482.
DrugBanki DB00760. Meropenem.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 16834474.

Miscellaneous databases

EvolutionaryTracei P14489.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn1404.
  2. "Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa."
    Maveyraud L., Golemi D., Kotra L.P., Tranier S., Vakulenko S., Mobashery S., Samama J.-P.
    Structure 8:1289-1298(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 21-266, CARBAMYLATION AT LYS-70.

Entry informationi

Entry nameiBLO10_PSEAI
AccessioniPrimary (citable) accession number: P14489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 26, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3