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P14489

- BLO10_PSEAI

UniProt

P14489 - BLO10_PSEAI

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Protein
Beta-lactamase OXA-10
Gene
bla, oxa10, pse2
Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes both carbenicillin and oxacillin.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Acyl-ester intermediate

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC
  2. penicillin binding Source: InterPro

GO - Biological processi

  1. antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXA-10 (EC:3.5.2.6)
Alternative name(s):
Beta-lactamase PSE-2
Gene namesi
Name:bla
Synonyms:oxa10, pse2
Encoded oniPlasmid pMON2340 Publication
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020
Add
BLAST
Chaini21 – 266246Beta-lactamase OXA-10
PRO_0000017030Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi44 ↔ 51
Modified residuei70 – 701N6-carboxylysine

Keywords - PTMi

Disulfide bond

Interactioni

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 243
Helixi26 – 283
Helixi29 – 335
Turni34 – 363
Beta strandi39 – 479
Beta strandi50 – 545
Helixi56 – 594
Helixi66 – 694
Helixi70 – 7910
Beta strandi81 – 833
Helixi100 – 1023
Helixi108 – 1136
Helixi117 – 12711
Helixi129 – 13810
Turni150 – 1523
Helixi153 – 1564
Helixi163 – 17412
Beta strandi178 – 1803
Helixi182 – 19110
Beta strandi193 – 1964
Beta strandi198 – 20811
Beta strandi214 – 2163
Beta strandi218 – 22811
Beta strandi231 – 24212
Helixi244 – 2485
Helixi249 – 26012

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E3UX-ray1.66A/B/C/D21-266[»]
1E4DX-ray1.80A/B/C/D21-266[»]
1EWZX-ray2.40A/B/C/D21-266[»]
1FOFX-ray2.00A/B20-265[»]
1K4EX-ray2.00A/B20-266[»]
1K4FX-ray1.60A/B20-266[»]
1K54X-ray1.70A/B/C/D21-266[»]
1K55X-ray1.39A/B/C/D21-266[»]
1K56X-ray1.70A/B/C/D21-266[»]
1K57X-ray1.90A/B/C/D21-266[»]
1K6RX-ray2.30A/B20-266[»]
1K6SX-ray2.03A/B20-266[»]
2HP5X-ray2.70A/B/C/D20-266[»]
2HP6X-ray2.20A/B20-266[»]
2HP9X-ray2.50A/B20-266[»]
2HPBX-ray2.05A/B20-266[»]
2RL3X-ray1.90A/B20-266[»]
2WGIX-ray2.85A/B21-266[»]
2WGVX-ray1.80A/B20-266[»]
2WGWX-ray1.80A/B20-266[»]
2WKHX-ray1.79A/B20-266[»]
2WKIX-ray2.10A/B20-266[»]
2X01X-ray1.90A/B20-266[»]
2X02X-ray1.35A/B20-266[»]
3LCEX-ray2.00A/B/C/D21-266[»]
ProteinModelPortaliP14489.
SMRiP14489. Positions 21-266.

Miscellaneous databases

EvolutionaryTraceiP14489.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 2073Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14489-1 [UniParc]FASTAAdd to Basket

« Hide

MKTFAAYVII ACLSSTALAG SITENTSWNK EFSAEAVNGV FVLCKSSSKS    50
CATNDLARAS KEYLPASTFK IPNAIIGLET GVIKNEHQVF KWDGKPRAMK 100
QWERDLTLRG AIQVSAVPVF QQIAREVGEV RMQKYLKKFS YGNQNISGGI 150
DKFWLEGQLR ISAVNQVEFL ESLYLNKLSA SKENQLIVKE ALVTEAAPEY 200
LVHSKTGFSG VGTESNPGVA WWVGWVEKET EVYFFAFNMD IDNESKLPLR 250
KSIPTKIMES EGIIGG 266
Length:266
Mass (Da):29,507
Last modified:January 1, 1990 - v1
Checksum:i9ABFF429D028F240
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37105 Genomic DNA. Translation: AAB60534.1.
J03427 Genomic DNA. Translation: AAA25648.1.
PIRiS06462.
RefSeqiYP_008532606.1. NC_022344.1.

Genome annotation databases

GeneIDi16834474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U37105 Genomic DNA. Translation: AAB60534.1 .
J03427 Genomic DNA. Translation: AAA25648.1 .
PIRi S06462.
RefSeqi YP_008532606.1. NC_022344.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E3U X-ray 1.66 A/B/C/D 21-266 [» ]
1E4D X-ray 1.80 A/B/C/D 21-266 [» ]
1EWZ X-ray 2.40 A/B/C/D 21-266 [» ]
1FOF X-ray 2.00 A/B 20-265 [» ]
1K4E X-ray 2.00 A/B 20-266 [» ]
1K4F X-ray 1.60 A/B 20-266 [» ]
1K54 X-ray 1.70 A/B/C/D 21-266 [» ]
1K55 X-ray 1.39 A/B/C/D 21-266 [» ]
1K56 X-ray 1.70 A/B/C/D 21-266 [» ]
1K57 X-ray 1.90 A/B/C/D 21-266 [» ]
1K6R X-ray 2.30 A/B 20-266 [» ]
1K6S X-ray 2.03 A/B 20-266 [» ]
2HP5 X-ray 2.70 A/B/C/D 20-266 [» ]
2HP6 X-ray 2.20 A/B 20-266 [» ]
2HP9 X-ray 2.50 A/B 20-266 [» ]
2HPB X-ray 2.05 A/B 20-266 [» ]
2RL3 X-ray 1.90 A/B 20-266 [» ]
2WGI X-ray 2.85 A/B 21-266 [» ]
2WGV X-ray 1.80 A/B 20-266 [» ]
2WGW X-ray 1.80 A/B 20-266 [» ]
2WKH X-ray 1.79 A/B 20-266 [» ]
2WKI X-ray 2.10 A/B 20-266 [» ]
2X01 X-ray 1.90 A/B 20-266 [» ]
2X02 X-ray 1.35 A/B 20-266 [» ]
3LCE X-ray 2.00 A/B/C/D 21-266 [» ]
ProteinModelPortali P14489.
SMRi P14489. Positions 21-266.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P14489.
ChEMBLi CHEMBL5482.
DrugBanki DB00760. Meropenem.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 16834474.

Miscellaneous databases

EvolutionaryTracei P14489.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view ]
Pfami PF00905. Transpeptidase. 1 hit.
[Graphical view ]
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Transposon: Tn1404.
  2. "Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa."
    Maveyraud L., Golemi D., Kotra L.P., Tranier S., Vakulenko S., Mobashery S., Samama J.-P.
    Structure 8:1289-1298(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 21-266, CARBAMYLATION AT LYS-70.

Entry informationi

Entry nameiBLO10_PSEAI
AccessioniPrimary (citable) accession number: P14489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 3, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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