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P14489

- BLO10_PSEAI

UniProt

P14489 - BLO10_PSEAI

Protein

Beta-lactamase OXA-10

Gene

bla

Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Hydrolyzes both carbenicillin and oxacillin.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei67 – 671Acyl-ester intermediate

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC
    2. penicillin binding Source: InterPro

    GO - Biological processi

    1. antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase OXA-10 (EC:3.5.2.6)
    Alternative name(s):
    Beta-lactamase PSE-2
    Gene namesi
    Name:bla
    Synonyms:oxa10, pse2
    Encoded oniPlasmid pMON2340 Publication
    OrganismiPseudomonas aeruginosa
    Taxonomic identifieri287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Add
    BLAST
    Chaini21 – 266246Beta-lactamase OXA-10PRO_0000017030Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi44 ↔ 51
    Modified residuei70 – 701N6-carboxylysine1 Publication

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Structurei

    Secondary structure

    1
    266
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi22 – 243
    Helixi26 – 283
    Helixi29 – 335
    Turni34 – 363
    Beta strandi39 – 479
    Beta strandi50 – 545
    Helixi56 – 594
    Helixi66 – 694
    Helixi70 – 7910
    Beta strandi81 – 833
    Helixi100 – 1023
    Helixi108 – 1136
    Helixi117 – 12711
    Helixi129 – 13810
    Turni150 – 1523
    Helixi153 – 1564
    Helixi163 – 17412
    Beta strandi178 – 1803
    Helixi182 – 19110
    Beta strandi193 – 1964
    Beta strandi198 – 20811
    Beta strandi214 – 2163
    Beta strandi218 – 22811
    Beta strandi231 – 24212
    Helixi244 – 2485
    Helixi249 – 26012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E3UX-ray1.66A/B/C/D21-266[»]
    1E4DX-ray1.80A/B/C/D21-266[»]
    1EWZX-ray2.40A/B/C/D21-266[»]
    1FOFX-ray2.00A/B20-265[»]
    1K4EX-ray2.00A/B20-266[»]
    1K4FX-ray1.60A/B20-266[»]
    1K54X-ray1.70A/B/C/D21-266[»]
    1K55X-ray1.39A/B/C/D21-266[»]
    1K56X-ray1.70A/B/C/D21-266[»]
    1K57X-ray1.90A/B/C/D21-266[»]
    1K6RX-ray2.30A/B20-266[»]
    1K6SX-ray2.03A/B20-266[»]
    2HP5X-ray2.70A/B/C/D20-266[»]
    2HP6X-ray2.20A/B20-266[»]
    2HP9X-ray2.50A/B20-266[»]
    2HPBX-ray2.05A/B20-266[»]
    2RL3X-ray1.90A/B20-266[»]
    2WGIX-ray2.85A/B21-266[»]
    2WGVX-ray1.80A/B20-266[»]
    2WGWX-ray1.80A/B20-266[»]
    2WKHX-ray1.79A/B20-266[»]
    2WKIX-ray2.10A/B20-266[»]
    2X01X-ray1.90A/B20-266[»]
    2X02X-ray1.35A/B20-266[»]
    3LCEX-ray2.00A/B/C/D21-266[»]
    ProteinModelPortaliP14489.
    SMRiP14489. Positions 21-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14489.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni205 – 2073Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-D beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR002137. Beta-lactam_class-D_AS.
    IPR001460. PCN-bd_Tpept.
    [Graphical view]
    PfamiPF00905. Transpeptidase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00337. BETA_LACTAMASE_D. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P14489-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTFAAYVII ACLSSTALAG SITENTSWNK EFSAEAVNGV FVLCKSSSKS    50
    CATNDLARAS KEYLPASTFK IPNAIIGLET GVIKNEHQVF KWDGKPRAMK 100
    QWERDLTLRG AIQVSAVPVF QQIAREVGEV RMQKYLKKFS YGNQNISGGI 150
    DKFWLEGQLR ISAVNQVEFL ESLYLNKLSA SKENQLIVKE ALVTEAAPEY 200
    LVHSKTGFSG VGTESNPGVA WWVGWVEKET EVYFFAFNMD IDNESKLPLR 250
    KSIPTKIMES EGIIGG 266
    Length:266
    Mass (Da):29,507
    Last modified:January 1, 1990 - v1
    Checksum:i9ABFF429D028F240
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37105 Genomic DNA. Translation: AAB60534.1.
    J03427 Genomic DNA. Translation: AAA25648.1.
    PIRiS06462.
    RefSeqiYP_008532606.1. NC_022344.1.

    Genome annotation databases

    GeneIDi16834474.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37105 Genomic DNA. Translation: AAB60534.1 .
    J03427 Genomic DNA. Translation: AAA25648.1 .
    PIRi S06462.
    RefSeqi YP_008532606.1. NC_022344.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E3U X-ray 1.66 A/B/C/D 21-266 [» ]
    1E4D X-ray 1.80 A/B/C/D 21-266 [» ]
    1EWZ X-ray 2.40 A/B/C/D 21-266 [» ]
    1FOF X-ray 2.00 A/B 20-265 [» ]
    1K4E X-ray 2.00 A/B 20-266 [» ]
    1K4F X-ray 1.60 A/B 20-266 [» ]
    1K54 X-ray 1.70 A/B/C/D 21-266 [» ]
    1K55 X-ray 1.39 A/B/C/D 21-266 [» ]
    1K56 X-ray 1.70 A/B/C/D 21-266 [» ]
    1K57 X-ray 1.90 A/B/C/D 21-266 [» ]
    1K6R X-ray 2.30 A/B 20-266 [» ]
    1K6S X-ray 2.03 A/B 20-266 [» ]
    2HP5 X-ray 2.70 A/B/C/D 20-266 [» ]
    2HP6 X-ray 2.20 A/B 20-266 [» ]
    2HP9 X-ray 2.50 A/B 20-266 [» ]
    2HPB X-ray 2.05 A/B 20-266 [» ]
    2RL3 X-ray 1.90 A/B 20-266 [» ]
    2WGI X-ray 2.85 A/B 21-266 [» ]
    2WGV X-ray 1.80 A/B 20-266 [» ]
    2WGW X-ray 1.80 A/B 20-266 [» ]
    2WKH X-ray 1.79 A/B 20-266 [» ]
    2WKI X-ray 2.10 A/B 20-266 [» ]
    2X01 X-ray 1.90 A/B 20-266 [» ]
    2X02 X-ray 1.35 A/B 20-266 [» ]
    3LCE X-ray 2.00 A/B/C/D 21-266 [» ]
    ProteinModelPortali P14489.
    SMRi P14489. Positions 21-266.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P14489.
    ChEMBLi CHEMBL5482.
    DrugBanki DB00760. Meropenem.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 16834474.

    Miscellaneous databases

    EvolutionaryTracei P14489.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR002137. Beta-lactam_class-D_AS.
    IPR001460. PCN-bd_Tpept.
    [Graphical view ]
    Pfami PF00905. Transpeptidase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00337. BETA_LACTAMASE_D. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Transposon: Tn1404.
    2. "Insights into class D beta-lactamases are revealed by the crystal structure of the OXA10 enzyme from Pseudomonas aeruginosa."
      Maveyraud L., Golemi D., Kotra L.P., Tranier S., Vakulenko S., Mobashery S., Samama J.-P.
      Structure 8:1289-1298(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.66 ANGSTROMS) OF 21-266, CARBAMYLATION AT LYS-70.

    Entry informationi

    Entry nameiBLO10_PSEAI
    AccessioniPrimary (citable) accession number: P14489
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Plasmid, Transposable element

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3