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Protein

Beta-lactamase OXA-10

Gene

bla

Organism
Pseudomonas aeruginosa
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes both carbenicillin and oxacillin.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei67Acyl-ester intermediate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BRENDAi3.5.2.6. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase OXA-10 (EC:3.5.2.6)
Alternative name(s):
Beta-lactamase PSE-2
Gene namesi
Name:bla
Synonyms:oxa10, pse2
Encoded oniPlasmid pMON2340 Publication
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5482.
DrugBankiDB00760. Meropenem.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Add BLAST20
ChainiPRO_000001703021 – 266Beta-lactamase OXA-10Add BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi44 ↔ 51
Modified residuei70N6-carboxylysine1 Publication1

Keywords - PTMi

Disulfide bond

Interactioni

Chemistry databases

BindingDBiP14489.

Structurei

Secondary structure

1266
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 24Combined sources3
Helixi26 – 28Combined sources3
Helixi29 – 33Combined sources5
Turni34 – 36Combined sources3
Beta strandi39 – 47Combined sources9
Beta strandi50 – 54Combined sources5
Helixi56 – 59Combined sources4
Helixi66 – 69Combined sources4
Helixi70 – 79Combined sources10
Beta strandi81 – 83Combined sources3
Helixi100 – 102Combined sources3
Helixi108 – 113Combined sources6
Helixi117 – 127Combined sources11
Helixi129 – 138Combined sources10
Turni150 – 152Combined sources3
Helixi153 – 156Combined sources4
Helixi163 – 174Combined sources12
Beta strandi178 – 180Combined sources3
Helixi182 – 191Combined sources10
Beta strandi193 – 196Combined sources4
Beta strandi198 – 208Combined sources11
Beta strandi214 – 216Combined sources3
Beta strandi218 – 228Combined sources11
Beta strandi231 – 242Combined sources12
Helixi244 – 248Combined sources5
Helixi249 – 260Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3UX-ray1.66A/B/C/D21-266[»]
1E4DX-ray1.80A/B/C/D21-266[»]
1EWZX-ray2.40A/B/C/D21-266[»]
1FOFX-ray2.00A/B20-265[»]
1K4EX-ray2.00A/B20-266[»]
1K4FX-ray1.60A/B20-266[»]
1K54X-ray1.70A/B/C/D21-266[»]
1K55X-ray1.39A/B/C/D21-266[»]
1K56X-ray1.70A/B/C/D21-266[»]
1K57X-ray1.90A/B/C/D21-266[»]
1K6RX-ray2.30A/B20-266[»]
1K6SX-ray2.03A/B20-266[»]
2HP5X-ray2.70A/B/C/D20-266[»]
2HP6X-ray2.20A/B20-266[»]
2HP9X-ray2.50A/B20-266[»]
2HPBX-ray2.05A/B20-266[»]
2RL3X-ray1.90A/B20-266[»]
2WGIX-ray2.85A/B21-266[»]
2WGVX-ray1.80A/B20-266[»]
2WGWX-ray1.80A/B20-266[»]
2WKHX-ray1.79A/B20-266[»]
2WKIX-ray2.10A/B20-266[»]
2X01X-ray1.90A/B20-266[»]
2X02X-ray1.35A/B20-266[»]
3LCEX-ray2.00A/B/C/D21-266[»]
4S2OX-ray1.70A/B20-265[»]
4WZ5X-ray1.60A/B/C/D20-266[»]
5FQ9X-ray1.50A/B20-266[»]
ProteinModelPortaliP14489.
SMRiP14489.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14489.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 207Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-D beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

KOiK18792.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14489-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTFAAYVII ACLSSTALAG SITENTSWNK EFSAEAVNGV FVLCKSSSKS
60 70 80 90 100
CATNDLARAS KEYLPASTFK IPNAIIGLET GVIKNEHQVF KWDGKPRAMK
110 120 130 140 150
QWERDLTLRG AIQVSAVPVF QQIAREVGEV RMQKYLKKFS YGNQNISGGI
160 170 180 190 200
DKFWLEGQLR ISAVNQVEFL ESLYLNKLSA SKENQLIVKE ALVTEAAPEY
210 220 230 240 250
LVHSKTGFSG VGTESNPGVA WWVGWVEKET EVYFFAFNMD IDNESKLPLR
260
KSIPTKIMES EGIIGG
Length:266
Mass (Da):29,507
Last modified:January 1, 1990 - v1
Checksum:i9ABFF429D028F240
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37105 Genomic DNA. Translation: AAB60534.1.
J03427 Genomic DNA. Translation: AAA25648.1.
PIRiS06462.
RefSeqiWP_000846390.1. NZ_LODH01000074.1.

Genome annotation databases

KEGGiag:AAB60534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37105 Genomic DNA. Translation: AAB60534.1.
J03427 Genomic DNA. Translation: AAA25648.1.
PIRiS06462.
RefSeqiWP_000846390.1. NZ_LODH01000074.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3UX-ray1.66A/B/C/D21-266[»]
1E4DX-ray1.80A/B/C/D21-266[»]
1EWZX-ray2.40A/B/C/D21-266[»]
1FOFX-ray2.00A/B20-265[»]
1K4EX-ray2.00A/B20-266[»]
1K4FX-ray1.60A/B20-266[»]
1K54X-ray1.70A/B/C/D21-266[»]
1K55X-ray1.39A/B/C/D21-266[»]
1K56X-ray1.70A/B/C/D21-266[»]
1K57X-ray1.90A/B/C/D21-266[»]
1K6RX-ray2.30A/B20-266[»]
1K6SX-ray2.03A/B20-266[»]
2HP5X-ray2.70A/B/C/D20-266[»]
2HP6X-ray2.20A/B20-266[»]
2HP9X-ray2.50A/B20-266[»]
2HPBX-ray2.05A/B20-266[»]
2RL3X-ray1.90A/B20-266[»]
2WGIX-ray2.85A/B21-266[»]
2WGVX-ray1.80A/B20-266[»]
2WGWX-ray1.80A/B20-266[»]
2WKHX-ray1.79A/B20-266[»]
2WKIX-ray2.10A/B20-266[»]
2X01X-ray1.90A/B20-266[»]
2X02X-ray1.35A/B20-266[»]
3LCEX-ray2.00A/B/C/D21-266[»]
4S2OX-ray1.70A/B20-265[»]
4WZ5X-ray1.60A/B/C/D20-266[»]
5FQ9X-ray1.50A/B20-266[»]
ProteinModelPortaliP14489.
SMRiP14489.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP14489.
ChEMBLiCHEMBL5482.
DrugBankiDB00760. Meropenem.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAB60534.

Phylogenomic databases

KOiK18792.

Enzyme and pathway databases

BRENDAi3.5.2.6. 5087.

Miscellaneous databases

EvolutionaryTraceiP14489.
PROiP14489.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR002137. Beta-lactam_class-D_AS.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00337. BETA_LACTAMASE_D. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLO10_PSEAI
AccessioniPrimary (citable) accession number: P14489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid, Transposable element

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.