P14483 (HB2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
H-2 class II histocompatibility antigen, A beta chain

Gene names

Name:	H2-Ab1
Synonyms:	H2-iabeta

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Post-translational modification	Ubiquitinated in immature dendritic cells leading to down-regulation of MHC class II. Ref.3 Ref.4
Sequence similarities	Belongs to the MHC class II family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Immunity
Cellular component	MHC II Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell affinity maturation Inferred from direct assay PubMed 1378867. Source: BHF-UCL antigen processing and presentation of exogenous peptide antigen via MHC class II Inferred from direct assay PubMed 11027433PubMed 11069069PubMed 11714799PubMed 7679955PubMed 8598040PubMed 8598041PubMed 9432982PubMed 9492004. Source: MGI cellular response to interferon-gamma Inferred from direct assay PubMed 1845803PubMed 2493384PubMed 2502420PubMed 7691725PubMed 9244347. Source: BHF-UCL positive regulation of T-helper 1 type immune response Inferred from direct assay PubMed 14607905. Source: BHF-UCL positive regulation of alpha-beta T cell activation Inferred from direct assay PubMed 14607905. Source: BHF-UCL positive regulation of antigen processing and presentation Inferred from direct assay PubMed 14607905. Source: BHF-UCL
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 7679955. Source: MGI MHC class II protein complex Inferred from direct assay PubMed 11148202PubMed 7679955PubMed 7985028PubMed 8598040PubMed 8598041PubMed 8638109PubMed 9432982PubMed 9492004. Source: MGI early endosome Inferred from direct assay PubMed 11854359. Source: MGI external side of plasma membrane Inferred from direct assay PubMed 11148202PubMed 12801066PubMed 14643301PubMed 7679955PubMed 8598040PubMed 8598041PubMed 8638109PubMed 9432982PubMed 9492004. Source: MGI integral to membrane Inferred from direct assay PubMed 7584146. Source: MGI multivesicular body Inferred from direct assay PubMed 11854359. Source: MGI
Molecular_function	peptide antigen binding Inferred from direct assay PubMed 11069069PubMed 9432982. Source: MGI toxic substance binding Inferred from physical interaction PubMed 2159298. Source: BHF-UCL transcription factor binding Inferred from direct assay PubMed 8611149. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Chain

28 – 265

238

H-2 class II histocompatibility antigen, A beta chain

PRO_0000018993

Regions

Topological domain

28 – 226

199

Extracellular Potential

Transmembrane

227 – 247

21

Helical; Potential

Topological domain

248 – 265

18

Cytoplasmic Potential

Domain

125 – 213

89

Ig-like C1-type

Region

28 – 122

95

Beta-1

Region

123 – 216

94

Beta-2

Region

217 – 226

10

Connecting peptide

Amino acid modifications

Glycosylation

46

1

N-linked (GlcNAc...) Potential

Disulfide bond

42 ↔ 106

By similarity

Disulfide bond

145 ↔ 201

By similarity

Secondary structure

1

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265

Helix Strand Turn

Details...

Beta strand

34 – 45

12

Turn

46 – 49

4

Beta strand

50 – 59

10

Beta strand

62 – 68

7

Turn

69 – 71

3

Beta strand

73 – 75

3

Helix

79 – 81

3

Helix

82 – 89

8

Helix

92 – 104

13

Helix

106 – 111

6

Helix

113 – 116

4

Helix

118 – 120

3

Beta strand

126 – 132

7

Turn

137 – 139

3

Beta strand

142 – 153

12

Beta strand

156 – 161

6

Beta strand

164 – 166

3

Beta strand

170 – 172

3

Beta strand

179 – 181

3

Beta strand

183 – 190

8

Beta strand

199 – 204

6

Beta strand

212 – 216

5

Sequences

	Sequence		Length	Mass (Da)
	P14483 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: DA5118187D254BD6	FASTA	265	30,128
	10 20 30 40 50 60 MALQIPSLLL SAAVVVLMVL SSPGTEGGDS ERHFVYQFMG ECYFTNGTQR IRYVTRYIYN 70 80 90 100 110 120 REEYVRYDSD VGEHRAVTEL GRPDAEYWNS QPEILERTRA ELDTVCRHNY EGPETHTSLR 130 140 150 160 170 180 RLEQPNVVIS LSRTEALNHH NTLVCSVTDF YPAKIKVRWF RNGQEETVGV SSTQLIRNGD 190 200 210 220 230 240 WTFQVLVMLE MTPRRGEVYT CHVEHPSLKS PITVEWRAQS ESAWSKMLSG IGGCVLGVIF 250 260 LGLGLFIRHR SQKGPRGPPP AGLLQ

References

[1]	"Structure of the murine immune response I-A beta locus: sequence of the I-A beta gene and an adjacent beta-chain second domain exon." Larhammar D., Hammerling U., Denaro M., Lund T., Flavell R.A., Rask L., Peterson P.A. Cell 34:179-188(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Rowen L., Qin S., Ahearn M.E., Loretz C., Faust J., Lasky S., Mahairas G., Hood L.E. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Dendritic cells regulate exposure of MHC class II at their plasma membrane by oligoubiquitination." van Niel G., Wubbolts R., Ten Broeke T., Buschow S.I., Ossendorp F.A., Melief C.J., Raposo G., van Balkom B.W., Stoorvogel W. Immunity 25:885-894(2006) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION.
[4]	"Surface expression of MHC class II in dendritic cells is controlled by regulated ubiquitination." Shin J.S., Ebersold M., Pypaert M., Delamarre L., Hartley A., Mellman I. Nature 444:115-118(2006) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V01527 Genomic DNA. Translation: CAA24768.1.
AF027865 Genomic DNA. Translation: AAB81531.1.

IPI

IPI00126585.

PIR

I48656.

RefSeq

NP_996988.2. NM_207105.3.

UniGene

Mm.254067.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LNU	X-ray	2.50	B/D/F/H	30-216	[»]
1MUJ	X-ray	2.15	B	28-216	[»]
3C5Z	X-ray	2.55	D/H	30-216	[»]
3C60	X-ray	3.05	D/H	30-216	[»]
3C6L	X-ray	3.40	D/H	30-216	[»]
3RDT	X-ray	2.70	D	27-218	[»]

ProteinModelPortal

P14483.

SMR

P14483. Positions 33-218.

ModBase

Search...

Protein-protein interaction databases

IntAct

P14483. 1 interaction.

MINT

MINT-257639.

Proteomic databases

PaxDb

P14483.

PRIDE

P14483.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000040828; ENSMUSP00000041008; ENSMUSG00000073421.

GeneID

14961.

KEGG

mmu:14961.

UCSC

uc008ccb.2. mouse.

Organism-specific databases

CTD

14961.

MGI

MGI:103070. H2-Ab1.

Phylogenomic databases

eggNOG

NOG68200.

HOGENOM

HOG000100909.

HOVERGEN

HBG012730.

InParanoid

P14483.

KO

K06752.

OMA

IRQRSQK.

OrthoDB

EOG461454.

Gene expression databases

ArrayExpress

P14483.

Bgee

P14483.

CleanEx

MM_H2-AB1.

Genevestigator

P14483.

GermOnline

ENSMUSG00000073421. Mus musculus.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
3.10.320.10. 1 hit.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR000353. MHC_II_b_N.
[Graphical view]

Pfam

PF07654. C1-set. 1 hit.
PF00969. MHC_II_beta. 1 hit.
[Graphical view]

ProDom

PD000328. MHC_II_b_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00407. IGc1. 1 hit.
SM00921. MHC_II_beta. 1 hit.
[Graphical view]

SUPFAM

SSF54452. MHC_I/II-like_Ag-recog. 1 hit.

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14483.

NextBio

287310.

SOURCE

Search...

Entry information

Entry name

HB2A_MOUSE

Accession

Primary (citable) accession number: P14483

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 29, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program