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Protein

H-2 class II histocompatibility antigen, A beta chain

Gene

H2-Ab1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. peptide antigen binding Source: MGI
  2. repressing transcription factor binding Source: BHF-UCL
  3. toxic substance binding Source: BHF-UCL
  4. transcription factor binding Source: BHF-UCL
  5. ubiquitin protein ligase binding Source: BHF-UCL

GO - Biological processi

  1. antigen processing and presentation Source: MGI
  2. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: MGI
  3. antigen processing and presentation of peptide antigen Source: MGI
  4. B cell affinity maturation Source: BHF-UCL
  5. cellular response to interferon-gamma Source: BHF-UCL
  6. humoral immune response mediated by circulating immunoglobulin Source: MGI
  7. immune response Source: MGI
  8. immunoglobulin production involved in immunoglobulin mediated immune response Source: MGI
  9. positive regulation of alpha-beta T cell activation Source: BHF-UCL
  10. positive regulation of antigen processing and presentation Source: BHF-UCL
  11. positive regulation of T-helper 1 type immune response Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Immunity

Enzyme and pathway databases

ReactomeiREACT_280649. Downstream TCR signaling.
REACT_309115. PD-1 signaling.
REACT_316383. MHC class II antigen presentation.
REACT_334692. Generation of second messenger molecules.
REACT_345130. Translocation of ZAP-70 to Immunological synapse.
REACT_345471. Phosphorylation of CD3 and TCR zeta chains.
REACT_349056. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
H-2 class II histocompatibility antigen, A beta chain
Gene namesi
Name:H2-Ab1
Synonyms:H2-iabeta
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:103070. H2-Ab1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 226199ExtracellularSequence AnalysisAdd
BLAST
Transmembranei227 – 24721HelicalSequence AnalysisAdd
BLAST
Topological domaini248 – 26518CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. early endosome Source: MGI
  2. external side of plasma membrane Source: MGI
  3. Golgi apparatus Source: MGI
  4. integral component of membrane Source: MGI
  5. intracellular Source: MGI
  6. membrane Source: MGI
  7. MHC class II protein complex Source: MGI
  8. multivesicular body Source: MGI
  9. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, MHC II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Add
BLAST
Chaini28 – 265238H-2 class II histocompatibility antigen, A beta chainPRO_0000018993Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 106PROSITE-ProRule annotation
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi145 ↔ 201PROSITE-ProRule annotation

Post-translational modificationi

Ubiquitinated in immature dendritic cells leading to down-regulation of MHC class II.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

MaxQBiP14483.
PaxDbiP14483.
PRIDEiP14483.

Expressioni

Gene expression databases

BgeeiP14483.
CleanExiMM_H2-AB1.
ExpressionAtlasiP14483. baseline and differential.
GenevestigatoriP14483.

Interactioni

Protein-protein interaction databases

BioGridi200147. 1 interaction.
IntActiP14483. 1 interaction.
MINTiMINT-257639.

Structurei

Secondary structure

1
265
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 4512Combined sources
Turni46 – 494Combined sources
Beta strandi50 – 5910Combined sources
Beta strandi62 – 687Combined sources
Turni69 – 713Combined sources
Beta strandi73 – 753Combined sources
Helixi79 – 813Combined sources
Helixi82 – 898Combined sources
Helixi92 – 10413Combined sources
Helixi106 – 1116Combined sources
Helixi113 – 1164Combined sources
Helixi118 – 1203Combined sources
Beta strandi126 – 1327Combined sources
Turni137 – 1393Combined sources
Beta strandi142 – 15312Combined sources
Beta strandi156 – 1616Combined sources
Beta strandi164 – 1663Combined sources
Beta strandi170 – 1723Combined sources
Beta strandi179 – 1813Combined sources
Beta strandi183 – 1908Combined sources
Beta strandi199 – 2046Combined sources
Beta strandi212 – 2165Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LNUX-ray2.50B/D/F/H27-216[»]
1MUJX-ray2.15B28-216[»]
3C5ZX-ray2.55D/H30-216[»]
3C60X-ray3.05D/H30-216[»]
3C6LX-ray3.40D/H30-216[»]
3RDTX-ray2.70D30-218[»]
4P23X-ray2.25D31-219[»]
4P46X-ray2.85D31-219[»]
4P5TX-ray3.26D/H27-218[»]
ProteinModelPortaliP14483.
SMRiP14483. Positions 33-218.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14483.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 21389Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni28 – 12295Beta-1Add
BLAST
Regioni123 – 21694Beta-2Add
BLAST
Regioni217 – 22610Connecting peptide

Sequence similaritiesi

Belongs to the MHC class II family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG68200.
HOGENOMiHOG000100909.
HOVERGENiHBG012730.
KOiK06752.
OMAiEMTPRRG.
OrthoDBiEOG7PS1GV.
PhylomeDBiP14483.
TreeFamiTF336626.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR000353. MHC_II_b_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00969. MHC_II_beta. 1 hit.
[Graphical view]
ProDomiPD000328. MHC_II_b_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00407. IGc1. 1 hit.
SM00921. MHC_II_beta. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQIPSLLL SAAVVVLMVL SSPGTEGGDS ERHFVYQFMG ECYFTNGTQR
60 70 80 90 100
IRYVTRYIYN REEYVRYDSD VGEHRAVTEL GRPDAEYWNS QPEILERTRA
110 120 130 140 150
ELDTVCRHNY EGPETHTSLR RLEQPNVVIS LSRTEALNHH NTLVCSVTDF
160 170 180 190 200
YPAKIKVRWF RNGQEETVGV SSTQLIRNGD WTFQVLVMLE MTPRRGEVYT
210 220 230 240 250
CHVEHPSLKS PITVEWRAQS ESAWSKMLSG IGGCVLGVIF LGLGLFIRHR
260
SQKGPRGPPP AGLLQ
Length:265
Mass (Da):30,128
Last modified:January 1, 1990 - v1
Checksum:iDA5118187D254BD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01527 Genomic DNA. Translation: CAA24768.1.
AF027865 Genomic DNA. Translation: AAB81531.1.
CCDSiCCDS37583.1.
PIRiI48656.
RefSeqiNP_996988.2. NM_207105.3.
UniGeneiMm.254067.

Genome annotation databases

EnsembliENSMUST00000040828; ENSMUSP00000041008; ENSMUSG00000073421.
GeneIDi14961.
KEGGimmu:14961.
UCSCiuc008ccb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01527 Genomic DNA. Translation: CAA24768.1.
AF027865 Genomic DNA. Translation: AAB81531.1.
CCDSiCCDS37583.1.
PIRiI48656.
RefSeqiNP_996988.2. NM_207105.3.
UniGeneiMm.254067.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LNUX-ray2.50B/D/F/H27-216[»]
1MUJX-ray2.15B28-216[»]
3C5ZX-ray2.55D/H30-216[»]
3C60X-ray3.05D/H30-216[»]
3C6LX-ray3.40D/H30-216[»]
3RDTX-ray2.70D30-218[»]
4P23X-ray2.25D31-219[»]
4P46X-ray2.85D31-219[»]
4P5TX-ray3.26D/H27-218[»]
ProteinModelPortaliP14483.
SMRiP14483. Positions 33-218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200147. 1 interaction.
IntActiP14483. 1 interaction.
MINTiMINT-257639.

Proteomic databases

MaxQBiP14483.
PaxDbiP14483.
PRIDEiP14483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040828; ENSMUSP00000041008; ENSMUSG00000073421.
GeneIDi14961.
KEGGimmu:14961.
UCSCiuc008ccb.2. mouse.

Organism-specific databases

CTDi14961.
MGIiMGI:103070. H2-Ab1.

Phylogenomic databases

eggNOGiNOG68200.
HOGENOMiHOG000100909.
HOVERGENiHBG012730.
KOiK06752.
OMAiEMTPRRG.
OrthoDBiEOG7PS1GV.
PhylomeDBiP14483.
TreeFamiTF336626.

Enzyme and pathway databases

ReactomeiREACT_280649. Downstream TCR signaling.
REACT_309115. PD-1 signaling.
REACT_316383. MHC class II antigen presentation.
REACT_334692. Generation of second messenger molecules.
REACT_345130. Translocation of ZAP-70 to Immunological synapse.
REACT_345471. Phosphorylation of CD3 and TCR zeta chains.
REACT_349056. Interferon gamma signaling.

Miscellaneous databases

EvolutionaryTraceiP14483.
NextBioi287310.
PROiP14483.
SOURCEiSearch...

Gene expression databases

BgeeiP14483.
CleanExiMM_H2-AB1.
ExpressionAtlasiP14483. baseline and differential.
GenevestigatoriP14483.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR000353. MHC_II_b_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00969. MHC_II_beta. 1 hit.
[Graphical view]
ProDomiPD000328. MHC_II_b_N. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00407. IGc1. 1 hit.
SM00921. MHC_II_beta. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structure of the murine immune response I-A beta locus: sequence of the I-A beta gene and an adjacent beta-chain second domain exon."
    Larhammar D., Hammerling U., Denaro M., Lund T., Flavell R.A., Rask L., Peterson P.A.
    Cell 34:179-188(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Rowen L., Qin S., Ahearn M.E., Loretz C., Faust J., Lasky S., Mahairas G., Hood L.E.
    Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Dendritic cells regulate exposure of MHC class II at their plasma membrane by oligoubiquitination."
    van Niel G., Wubbolts R., Ten Broeke T., Buschow S.I., Ossendorp F.A., Melief C.J., Raposo G., van Balkom B.W., Stoorvogel W.
    Immunity 25:885-894(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.
  4. "Surface expression of MHC class II in dendritic cells is controlled by regulated ubiquitination."
    Shin J.S., Ebersold M., Pypaert M., Delamarre L., Hartley A., Mellman I.
    Nature 444:115-118(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.

Entry informationi

Entry nameiHB2A_MOUSE
AccessioniPrimary (citable) accession number: P14483
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 1, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.