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P14448 (FIBA_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fibrinogen alpha chain

Cleaved into the following 2 chains:

  1. Fibrinopeptide A
  2. Fibrinogen alpha chain
Gene names
Name:FGA
OrganismGallus gallus (Chicken)
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Subunit structure

Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain By similarity.

Subcellular location

Secreted.

Domain

A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.

Post-translational modification

Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.

Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.

Sequence similarities

Contains 1 fibrinogen C-terminal domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P14448-1)

Also known as: Alpha-E;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P14448-2)

Also known as: Alpha;

The sequence of this isoform differs from the canonical sequence as follows:
     506-509: DCDD → GTQK
     510-741: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Ref.3
Peptide19 – 3315Fibrinopeptide A Ref.3
PRO_0000009046
Chain34 – 741708Fibrinogen alpha chain
PRO_0000009047

Regions

Domain498 – 739242Fibrinogen C-terminal
Coiled coil67 – 506440

Sites

Site33 – 342Cleavage; by thrombin; to release fibrinopeptide A

Amino acid modifications

Modified residue191Pyrrolidone carboxylic acid
Disulfide bond46Interchain (with alpha chain)
Disulfide bond55Interchain (with beta chain)
Disulfide bond64Interchain (with gamma chain)
Disulfide bond68Interchain (with beta chain)
Disulfide bond180Interchain (with gamma chain)
Disulfide bond184Interchain (with beta chain)
Disulfide bond310 ↔ 341 By similarity

Natural variations

Alternative sequence506 – 5094DCDD → GTQK in isoform 2.
VSP_001535
Alternative sequence510 – 741232Missing in isoform 2.
VSP_001536

Secondary structure

1 .............. 741
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Alpha-E) [UniParc].

Last modified October 1, 1996. Version 4.
Checksum: A09F5F4F186DE3A6

FASTA74182,438
        10         20         30         40         50         60 
MIPVTILCVL LCLNLAWAQD GKTTFEKEGG GGRGPRILEN MHESSCKYEK NWPICVDDDW 

        70         80         90        100        110        120 
GTKCPSCCRM QGIIDDTDQN YSQRIDNIRQ QLADSQNKYK TSNRVIVETI NILKPGLEGA 

       130        140        150        160        170        180 
QQLDENYGHV STELRRRIVT LKQRVATQVN RIKALQNSIQ EQVVEMKRLE VDIDIKIRAC 

       190        200        210        220        230        240 
KGSCARSFDY QVDKEGYDNI QKHLTQASSI DMHPDFQTTT LSTLKMRPLK DSNVPEHFKL 

       250        260        270        280        290        300 
KPSPEMQAMS AFNNIKQMQV VLERPETDHV AEARGDSSPS HTGKLITSSH RRESPSLVDK 

       310        320        330        340        350        360 
TSSASSVHRC TRTVTKKVIS GPDGPREEIV EKMVSSDGSD CSHLQGGREG STYHFSGTGD 

       370        380        390        400        410        420 
FHKLDRLLPD LESFFTHDSV STSSRHSIGS STSSHVTGAG SSHLGTGGKD KFTDLGEEEE 

       430        440        450        460        470        480 
DDFGGLQPSG FAAGSASHSK TVLTSSSSSF NKGGSTFETK SLKTRETSEQ LGGVQHDQSA 

       490        500        510        520        530        540 
EDTPDFKARS FRPAAMSTRR SYNGKDCDDI RQKHTSGAKS GIFKIKPEGS NKVLSVYCDQ 

       550        560        570        580        590        600 
ETTLGGWLLI QQRMDGSVNF NRTWQDYRRG FGSVDGKGQG ELWLGNENIH LLTQNDTLLR 

       610        620        630        640        650        660 
VELEDWDGNA AYAEYIVQVG TEAEGYALTV SSYEGTAGDA LVAGWLEEGS EYTSHAQMQF 

       670        680        690        700        710        720 
STFDRDQDHW EESCAEVYGG GWWYNSCQAA NLNGIYYPGG HYDPRYNVPY EIENGVVWIP 

       730        740 
FRASDYSLKV VRMKIRPLET L

« Hide

Isoform 2 (Alpha) [UniParc].

Checksum: F102655C269D290B
Show »

FASTA50956,174

References

[1]Greininger G.
Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 1-4.
[2]"Bipartite mRNA for chicken alpha-fibrinogen potentially encodes an amino acid sequence homologous to beta- and gamma-fibrinogens."
Weissbach L., Grieninger G.
Proc. Natl. Acad. Sci. U.S.A. 87:5198-5202(1990) [PubMed: 2367530] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-741.
[3]"Amino acid sequence of chicken fibrinopeptide A."
Takagi T., Finlayson J.S., Iwanaga S.
Biochim. Biophys. Acta 534:161-164(1978) [PubMed: 656462] [Abstract]
Cited for: PROTEIN SEQUENCE OF 19-33.
[4]"Crystal structure of native chicken fibrinogen at 5.5-A resolution."
Yang Z., Mochalkin I., Veerapandian L., Riley M., Doolittle R.F.
Proc. Natl. Acad. Sci. U.S.A. 97:3907-3912(2000) [PubMed: 10737772] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF 19-509.
[5]"Crystal structure of native chicken fibrinogen at 2.7 A resolution."
Yang Z., Kollman J.M., Pandi L., Doolittle R.F.
Biochemistry 40:12515-12523(2001) [PubMed: 11601975] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 19-509.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U20803 expand/collapse EMBL AC list , U20799, U20800, U20801, U20802 Genomic DNA. Translation: AAB60686.1.
U20803 expand/collapse EMBL AC list , U20799, U20800, U20801, U20802 Genomic DNA. Translation: AAB60685.1.
M34096 mRNA. Translation: AAA99306.1.
M34096 mRNA. Translation: AAA99307.1.
IPIIPI00571323.
IPI00589899.
UniGeneGga.34280.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EI3X-ray5.50A/D19-505[»]
1M1JX-ray2.70A/D19-505[»]
ProteinModelPortalP14448.
SMRP14448. Positions 45-238, 545-740.
DisProtDP00233.
ModBaseSearch...

Protein-protein interaction databases

STRINGP14448.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGveNOG06112.
GeneTreeENSGT00600000084399.
HOGENOMHBG716848.
HOVERGENHBG005668.
InParanoidP14448.
OrthoDBEOG4XPQFB.

Family and domain databases

InterProIPR002181. Fibrinogen_a/b/g_C.
IPR014716. Fibrinogen_a/b/g_C_1.
IPR014715. Fibrinogen_a/b/g_C_2.
IPR012290. Fibrinogen_a/b/g_coil_dom.
IPR021996. Fibrinogen_aC.
IPR020837. Fibrinogen_CS.
[Graphical view]
Gene3DG3DSA:3.90.215.10. Fibrinogen_a/b/g_C_1. 1 hit.
G3DSA:4.10.530.10. Fibrinogen_a/b/g_C_2. 1 hit.
G3DSA:1.20.5.50. Fibrinogen_a/b/g_coil. 1 hit.
PfamPF08702. Fib_alpha. 1 hit.
PF12160. Fibrinogen_aC. 1 hit.
PF00147. Fibrinogen_C. 1 hit.
[Graphical view]
SMARTSM00186. FBG. 1 hit.
[Graphical view]
SUPFAMSSF56496. Fibrinogen_a/b/g_C. 1 hit.
PROSITEPS00514. FIBRINOGEN_C_1. 1 hit.
PS51406. FIBRINOGEN_C_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFIBA_CHICK
AccessionPrimary (citable) accession number: P14448
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 1, 1996
Last modified: September 21, 2011
This is version 95 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents