P14434 (HA2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
H-2 class II histocompatibility antigen, A-B alpha chain
Short name=IAalpha

Gene names

Name:

H2-Aa

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the MHC class II family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Immunity
Cellular component	MHC II Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II Inferred from direct assay PubMed 11027433PubMed 11069069PubMed 11714799PubMed 7679955PubMed 8598040PubMed 8598041PubMed 9432982PubMed 9492004. Source: MGI positive regulation of T cell differentiation Inferred from direct assay PubMed 8598041. Source: MGI response to interferon-gamma Inferred from direct assay PubMed 11465104. Source: BHF-UCL
Cellular_component	MHC class II protein complex Inferred from direct assay PubMed 11148202PubMed 7679955PubMed 7985028PubMed 8598040PubMed 8598041PubMed 8638109PubMed 9492004. Source: MGI external side of plasma membrane Inferred from direct assay PubMed 11148202PubMed 7679955PubMed 8598040PubMed 8598041PubMed 8638109PubMed 9432982PubMed 9492004. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosome Inferred from direct assay PubMed 11854359. Source: MGI
Molecular_function	peptide antigen binding Inferred from direct assay PubMed 11069069PubMed 9432982. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

Potential

Chain

24 – 256

233

H-2 class II histocompatibility antigen, A-B alpha chain

PRO_0000018975

Regions

Topological domain

24 – 218

195

Extracellular Potential

Transmembrane

219 – 244

26

Helical; Potential

Topological domain

245 – 256

12

Cytoplasmic Potential

Domain

114 – 206

93

Ig-like C1-type

Region

24 – 111

88

Alpha-1

Region

112 – 205

94

Alpha-2

Region

206 – 218

13

Connecting peptide

Amino acid modifications

Glycosylation

145

1

N-linked (GlcNAc...) Ref.5

Disulfide bond

134 ↔ 190

Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

256

Helix Strand Turn

Details...

Beta strand

30 – 42

13

Turn

43 – 45

3

Beta strand

46 – 53

8

Beta strand

56 – 62

7

Turn

63 – 66

4

Beta strand

67 – 72

6

Helix

73 – 76

4

Helix

83 – 103

21

Turn

104 – 106

3

Beta strand

115 – 122

8

Beta strand

126 – 128

3

Beta strand

130 – 139

10

Beta strand

145 – 154

10

Beta strand

157 – 161

5

Beta strand

165 – 167

3

Turn

168 – 170

3

Beta strand

172 – 180

9

Beta strand

188 – 193

6

Beta strand

197 – 199

3

Beta strand

201 – 205

5

Sequences

	Sequence		Length	Mass (Da)
	P14434 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: C9DD084F6179B41F	FASTA	256	28,093
	10 20 30 40 50 60 MPRSRALILG VLALTTMLSL CGGEDDIEAD HVGTYGISVY QSPGDIGQYT FEFDGDELFY 70 80 90 100 110 120 VDLDKKETVW MLPEFGQLAS FDPQGGLQNI AVVKHNLGVL TKRSNSTPAT NEAPQATVFP 130 140 150 160 170 180 KSPVLLGQPN TLICFVDNIF PPVINITWLR NSKSVADGVY ETSFFVNRDY SFHKLSYLTF 190 200 210 220 230 240 IPSDDDIYDC KVEHWGLEEP VLKHWEPEIP APMSELTETV VCALGLSVGL VGIVVGTIFI 250 IQGLRSGGTS RHPGPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the mouse major histocompatibility locus class II region." Rowen L., Qin S., Ahearn M.E., Loretz C., Faust J., Lasky S., Mahairas G., Hood L.E. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Sequence of the mouse major histocompatibility class II region." Rowen L., Qin S., Loretz C., Mix L., Lasky S., Madan A., Hood L.E. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[4]	"Regions of allelic hypervariability in the murine A alpha immune response gene." Benoist C.O., Mathis D.J., Kanter M.R., Williams V.E., McDevitt H.O. Cell 34:169-177(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-256.
[5]	"Crystal structure of MHC class II I-Ab in complex with a human CLIP peptide: prediction of an I-Ab peptide-binding motif." Zhu Y., Rudensky A.Y., Corper A.L., Teyton L., Wilson I.A. J. Mol. Biol. 326:1157-1174(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 24-205 IN COMPLEX WITH HUMAN CLIP PEPTIDE, GLYCOSYLATION AT ASN-145, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF027865 Genomic DNA. Translation: AAB81529.1.
AF050157 Genomic DNA. Translation: AAC05285.1.
BC019721 mRNA. Translation: AAH19721.1.
BC031711 mRNA. Translation: AAH31711.1.
K01922 mRNA. Translation: AAA39614.1.

IPI

IPI00126346.

RefSeq

NP_034508.2. NM_010378.2.

UniGene

Mm.235338.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LNU	X-ray	2.50	A/C/E/G	27-208	[»]
1MUJ	X-ray	2.15	A	24-205	[»]
3C5Z	X-ray	2.55	C/G	27-208	[»]
3C60	X-ray	3.05	C/G	27-208	[»]
3C6L	X-ray	3.40	C/G	27-208	[»]
3RDT	X-ray	2.70	C	27-208	[»]

ProteinModelPortal

P14434.

SMR

P14434. Positions 26-205.

ModBase

Search...

Protein-protein interaction databases

IntAct

P14434. 1 interaction.

MINT

MINT-257614.

Proteomic databases

PaxDb

P14434.

PRIDE

P14434.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000040655; ENSMUSP00000046105; ENSMUSG00000036594.

GeneID

14960.

KEGG

mmu:14960.

Organism-specific databases

CTD

14960.

MGI

MGI:95895. H2-Aa.

Phylogenomic databases

eggNOG

NOG26577.

GeneTree

ENSGT00560000076735.

HOGENOM

HOG000112076.

HOVERGEN

HBG006862.

InParanoid

P14434.

KO

K06752.

OMA

TVFSKFP.

OrthoDB

EOG4KWJTP.

Gene expression databases

ArrayExpress

P14434.

Bgee

P14434.

CleanEx

MM_H2-AA.

Genevestigator

P14434.

GermOnline

ENSMUSG00000036594. Mus musculus.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
3.10.320.10. 1 hit.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]

Pfam

PF07654. C1-set. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]

SMART

SM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]

SUPFAM

SSF54452. MHC_I/II-like_Ag-recog. 1 hit.

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14434.

NextBio

287306.

SOURCE

Search...

Entry information

Entry name

HA2B_MOUSE

Accession

Primary (citable) accession number: P14434
Secondary accession number(s): O78195

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	May 30, 2000
Last modified:	April 3, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program