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Protein

H-2 class II histocompatibility antigen, A-B alpha chain

Gene

H2-Aa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • peptide antigen binding Source: MGI

GO - Biological processi

  • antigen processing and presentation Source: MGI
  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: MGI
  • antigen processing and presentation of peptide antigen Source: MGI
  • positive regulation of T cell differentiation Source: MGI
  • response to interferon-gamma Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Immunity

Enzyme and pathway databases

ReactomeiREACT_280649. Downstream TCR signaling.
REACT_309115. PD-1 signaling.
REACT_316383. MHC class II antigen presentation.
REACT_334692. Generation of second messenger molecules.
REACT_345130. Translocation of ZAP-70 to Immunological synapse.
REACT_345471. Phosphorylation of CD3 and TCR zeta chains.
REACT_349056. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
H-2 class II histocompatibility antigen, A-B alpha chain
Short name:
IAalpha
Gene namesi
Name:H2-Aa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:95895. H2-Aa.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 218195ExtracellularSequence AnalysisAdd
BLAST
Transmembranei219 – 24426HelicalSequence AnalysisAdd
BLAST
Topological domaini245 – 25612CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • lysosome Source: MGI
  • membrane Source: MGI
  • MHC class II protein complex Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, MHC II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 256233H-2 class II histocompatibility antigen, A-B alpha chainPRO_0000018975Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi134 ↔ 190PROSITE-ProRule annotation1 Publication
Glycosylationi145 – 1451N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP14434.
PaxDbiP14434.
PRIDEiP14434.

Expressioni

Gene expression databases

BgeeiP14434.
CleanExiMM_H2-AA.
ExpressionAtlasiP14434. baseline and differential.
GenevisibleiP14434. MM.

Interactioni

Protein-protein interaction databases

BioGridi200146. 1 interaction.
IntActiP14434. 1 interaction.
MINTiMINT-257614.
STRINGi10090.ENSMUSP00000046105.

Structurei

Secondary structure

1
256
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 4213Combined sources
Turni43 – 453Combined sources
Beta strandi46 – 538Combined sources
Beta strandi56 – 627Combined sources
Turni63 – 664Combined sources
Beta strandi67 – 726Combined sources
Helixi73 – 764Combined sources
Helixi83 – 10321Combined sources
Turni104 – 1063Combined sources
Beta strandi115 – 1228Combined sources
Beta strandi126 – 1283Combined sources
Beta strandi130 – 13910Combined sources
Beta strandi145 – 15410Combined sources
Beta strandi157 – 1615Combined sources
Beta strandi165 – 1673Combined sources
Turni168 – 1703Combined sources
Beta strandi172 – 1809Combined sources
Beta strandi188 – 1936Combined sources
Beta strandi197 – 1993Combined sources
Beta strandi201 – 2055Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LNUX-ray2.50A/C/E/G27-208[»]
1MUJX-ray2.15A24-205[»]
3C5ZX-ray2.55C/G27-208[»]
3C60X-ray3.05C/G27-208[»]
3C6LX-ray3.40C/G27-208[»]
3RDTX-ray2.70C27-208[»]
4P23X-ray2.25C27-205[»]
4P46X-ray2.85C27-205[»]
4P5TX-ray3.26C/G27-208[»]
ProteinModelPortaliP14434.
SMRiP14434. Positions 26-205.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14434.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 20693Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 11188Alpha-1Add
BLAST
Regioni112 – 20594Alpha-2Add
BLAST
Regioni206 – 21813Connecting peptideAdd
BLAST

Sequence similaritiesi

Belongs to the MHC class II family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG26577.
GeneTreeiENSGT00760000118970.
HOGENOMiHOG000112076.
HOVERGENiHBG006862.
KOiK06752.
OMAiSYGVNVY.
OrthoDBiEOG7C2R26.
PhylomeDBiP14434.
TreeFamiTF333797.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]
SMARTiSM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRSRALILG VLALTTMLSL CGGEDDIEAD HVGTYGISVY QSPGDIGQYT
60 70 80 90 100
FEFDGDELFY VDLDKKETVW MLPEFGQLAS FDPQGGLQNI AVVKHNLGVL
110 120 130 140 150
TKRSNSTPAT NEAPQATVFP KSPVLLGQPN TLICFVDNIF PPVINITWLR
160 170 180 190 200
NSKSVADGVY ETSFFVNRDY SFHKLSYLTF IPSDDDIYDC KVEHWGLEEP
210 220 230 240 250
VLKHWEPEIP APMSELTETV VCALGLSVGL VGIVVGTIFI IQGLRSGGTS

RHPGPL
Length:256
Mass (Da):28,093
Last modified:May 30, 2000 - v2
Checksum:iC9DD084F6179B41F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027865 Genomic DNA. Translation: AAB81529.1.
AF050157 Genomic DNA. Translation: AAC05285.1.
BC019721 mRNA. Translation: AAH19721.1.
BC031711 mRNA. Translation: AAH31711.1.
K01922 mRNA. Translation: AAA39614.1.
CCDSiCCDS28645.1.
RefSeqiNP_034508.2. NM_010378.2.
UniGeneiMm.235338.

Genome annotation databases

EnsembliENSMUST00000040655; ENSMUSP00000046105; ENSMUSG00000036594.
GeneIDi14960.
KEGGimmu:14960.
UCSCiuc008ccd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027865 Genomic DNA. Translation: AAB81529.1.
AF050157 Genomic DNA. Translation: AAC05285.1.
BC019721 mRNA. Translation: AAH19721.1.
BC031711 mRNA. Translation: AAH31711.1.
K01922 mRNA. Translation: AAA39614.1.
CCDSiCCDS28645.1.
RefSeqiNP_034508.2. NM_010378.2.
UniGeneiMm.235338.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LNUX-ray2.50A/C/E/G27-208[»]
1MUJX-ray2.15A24-205[»]
3C5ZX-ray2.55C/G27-208[»]
3C60X-ray3.05C/G27-208[»]
3C6LX-ray3.40C/G27-208[»]
3RDTX-ray2.70C27-208[»]
4P23X-ray2.25C27-205[»]
4P46X-ray2.85C27-205[»]
4P5TX-ray3.26C/G27-208[»]
ProteinModelPortaliP14434.
SMRiP14434. Positions 26-205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200146. 1 interaction.
IntActiP14434. 1 interaction.
MINTiMINT-257614.
STRINGi10090.ENSMUSP00000046105.

Proteomic databases

MaxQBiP14434.
PaxDbiP14434.
PRIDEiP14434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040655; ENSMUSP00000046105; ENSMUSG00000036594.
GeneIDi14960.
KEGGimmu:14960.
UCSCiuc008ccd.1. mouse.

Organism-specific databases

CTDi14960.
MGIiMGI:95895. H2-Aa.

Phylogenomic databases

eggNOGiNOG26577.
GeneTreeiENSGT00760000118970.
HOGENOMiHOG000112076.
HOVERGENiHBG006862.
KOiK06752.
OMAiSYGVNVY.
OrthoDBiEOG7C2R26.
PhylomeDBiP14434.
TreeFamiTF333797.

Enzyme and pathway databases

ReactomeiREACT_280649. Downstream TCR signaling.
REACT_309115. PD-1 signaling.
REACT_316383. MHC class II antigen presentation.
REACT_334692. Generation of second messenger molecules.
REACT_345130. Translocation of ZAP-70 to Immunological synapse.
REACT_345471. Phosphorylation of CD3 and TCR zeta chains.
REACT_349056. Interferon gamma signaling.

Miscellaneous databases

EvolutionaryTraceiP14434.
NextBioi287306.
SOURCEiSearch...

Gene expression databases

BgeeiP14434.
CleanExiMM_H2-AA.
ExpressionAtlasiP14434. baseline and differential.
GenevisibleiP14434. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.10.320.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011162. MHC_I/II-like_Ag-recog.
IPR014745. MHC_II_a/b_N.
IPR001003. MHC_II_a_N.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00993. MHC_II_alpha. 1 hit.
[Graphical view]
SMARTiSM00407. IGc1. 1 hit.
SM00920. MHC_II_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the mouse major histocompatibility locus class II region."
    Rowen L., Qin S., Ahearn M.E., Loretz C., Faust J., Lasky S., Mahairas G., Hood L.E.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Sequence of the mouse major histocompatibility class II region."
    Rowen L., Qin S., Loretz C., Mix L., Lasky S., Madan A., Hood L.E.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  4. "Regions of allelic hypervariability in the murine A alpha immune response gene."
    Benoist C.O., Mathis D.J., Kanter M.R., Williams V.E., McDevitt H.O.
    Cell 34:169-177(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 9-256.
  5. "Crystal structure of MHC class II I-Ab in complex with a human CLIP peptide: prediction of an I-Ab peptide-binding motif."
    Zhu Y., Rudensky A.Y., Corper A.L., Teyton L., Wilson I.A.
    J. Mol. Biol. 326:1157-1174(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 24-205 IN COMPLEX WITH HUMAN CLIP PEPTIDE, GLYCOSYLATION AT ASN-145, DISULFIDE BONDS.

Entry informationi

Entry nameiHA2B_MOUSE
AccessioniPrimary (citable) accession number: P14434
Secondary accession number(s): O78195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: May 30, 2000
Last modified: June 24, 2015
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.