P14426 (HA13_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
H-2 class I histocompatibility antigen, D-K alpha chain
Short name=H-2D(K)

Gene names

Name:

H2-D1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	polyubiquitinated in case of infection by murid herpesvirus 4, by the viral E3 ligase K3 (mK3), leading to target the protein for rapid degradation by the endoplasmic reticulum-associated degradation (ERAD) system. Ubiquitination takes place on lysine, as well as serine and threonine residues present in the cytoplasmic tail. Hydroxylated serine and threonine residues in the cytoplasmic tail are subject to ubiquitination via ester bonds instead of the classical isopeptide linkage Probable. Hydroxylation of residues in the cytoplasmic tail By similarity.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Immunity
Cellular component	MHC I Membrane
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Hydroxylation Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of peptide antigen via MHC class I Inferred from electronic annotation. Source: UniProtKB-KW immune response Inferred from electronic annotation. Source: InterPro
Cellular_component	MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW integral to lumenal side of endoplasmic reticulum membrane Traceable author statement. Source: Reactome phagocytic vesicle membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	By similarity
	Chain	25 – 362	338	H-2 class I histocompatibility antigen, D-K alpha chain		PRO_0000018925
Regions
	Topological domain	25 – 306	282	Extracellular Potential
	Transmembrane	307 – 333	27	Helical; Potential
	Topological domain	334 – 362	29	Cytoplasmic Potential
	Domain	209 – 297	89	Ig-like C1-type
	Region	25 – 114	90	Alpha-1
	Region	115 – 206	92	Alpha-2
	Region	207 – 298	92	Alpha-3
	Region	299 – 306	8	Connecting peptide
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	200	1	N-linked (GlcNAc...) Ref.3
	Glycosylation	280	1	N-linked (GlcNAc...) Potential
	Disulfide bond	125 ↔ 188		By similarity
	Disulfide bond	227 ↔ 283		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14426 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: FF071720457D084D	FASTA	362	40,620
	10 20 30 40 50 60 MGAMVPRTLL LLLAAALAPA QTRAGPHSLR YFETVVSRPG LGEPRFISVG YVDNTEFVRF 70 80 90 100 110 120 DSDAENPRDE PRVRWMEQEG PEYWERETQI AKGNEQSFRV DLRTLLRYYN QSEGGSHTIQ 130 140 150 160 170 180 RLSGCDVGSD WRLLRGYEQF AYDGCDYIAL NEDLKTWTAA DMAALITKHK WEQAGAAERD 190 200 210 220 230 240 RAYLEGTCVE WLRRYLELGN ATLLHTDSPK AHVTHHPRSK VEVTLRCWAL GFYPADITLT 250 260 270 280 290 300 WQLNGEELTQ DMELVETRPA GDGTFQKWAS VVVPLGKEQN YTCHVYHEGL PEPLTLRWEP 310 320 330 340 350 360 PPSTDSYMVI VAVLGVLGAV AIIGAVVAFV MMMRRNTGGK GGDYTLTPGS QSSEMSLPDC KA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence analysis of the C3H H-2Kk and H-2Dk loci. Evolutionary relationships to H-2 genes from four other mouse strains." Watts S., Vogel J.M., Harriman W.D., Itoh T., Stauss H.J., Goodenow R.S. J. Immunol. 139:3878-3885(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C3H.
[2]	"MHC class I ubiquitination by a viral PHD/LAP finger protein." Boname J.M., Stevenson P.G. Immunity 15:627-636(2001) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION.
[3]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-110 AND ASN-200, MASS SPECTROMETRY. Tissue: Myoblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M18524 Genomic DNA. Translation: AAA53201.1.
IPI	IPI00126300.
PIR	I71998.
UniGene	Mm.422886. Mm.439675.
3D structure databases
ProteinModelPortal	P14426.
SMR	P14426. Positions 25-302.
ModBase	Search...
PTM databases
PhosphoSite	P14426.
Proteomic databases
PRIDE	P14426.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:95896. H2-D1.
Phylogenomic databases
HOVERGEN	HBG016709.
Enzyme and pathway databases
Reactome	REACT_102124. Immune System. REACT_107772. Immune System.
Gene expression databases
CleanEx	MM_H2-D1.
Genevestigator	P14426.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.30.500.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR003597. Ig_C1-set. IPR011161. MHC_I-like_Ag-recog. IPR011162. MHC_I/II-like_Ag-recog. IPR001039. MHC_I_a_a1/a2. IPR010579. MHC_I_a_C. [Graphical view]
Pfam	PF07654. C1-set. 1 hit. PF00129. MHC_I. 1 hit. PF06623. MHC_I_C. 1 hit. [Graphical view]
PRINTS	PR01638. MHCCLASSI.
SMART	SM00407. IGc1. 1 hit. [Graphical view]
SUPFAM	SSF54452. MHC_I/II-like_Ag-recog. 1 hit.
PROSITE	PS50835. IG_LIKE. 1 hit. PS00290. IG_MHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

HA13_MOUSE

Accession

Primary (citable) accession number: P14426

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program